UniProtKB - P69795 (PTQB_ECOLI)
Protein
PTS system N,N'-diacetylchitobiose-specific EIIB component
Gene
chbB
Organism
Escherichia coli (strain K12)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913117). Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117, PubMed:10913119).1 Publication2 Publications
Caution
Was originally (PubMed:2092358) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication
Catalytic activityi
- Nπ-phospho-L-histidyl-[protein] + N,N'-diacetylchitobiose(out) = diacetylchitobiose-6'-phosphate(in) + L-histidyl-[protein]2 PublicationsEC:2.7.1.1962 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 10 | Phosphocysteine intermediate5 Publications2 Publications | 1 |
GO - Molecular functioni
- kinase activity Source: UniProtKB-KW
- protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: InterPro
- protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity Source: EcoCyc
GO - Biological processi
- N,N'-diacetylchitobiose import Source: EcoCyc
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:CELA-MONOMER MetaCyc:CELA-MONOMER |
Protein family/group databases
TCDBi | 4.A.3.2.1, the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system N,N'-diacetylchitobiose-specific EIIB component1 PublicationAlternative name(s): EIIB-Chb1 Publication IVcel1 Publication N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component1 Publication (EC:2.7.1.1962 Publications) |
Gene namesi | Name:chbB1 Publication Synonyms:celA1 Publication Ordered Locus Names:b1738, JW1727 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | C → S: Unable to be phosphorylated by EIIA. 2 Publications | 1 |
Chemistry databases
DrugBanki | DB03544, S-Phosphocysteine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186489 | 1 – 106 | PTS system N,N'-diacetylchitobiose-specific EIIB componentAdd BLAST | 106 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | Phosphocysteine; by EIIAPROSITE-ProRule annotation1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P69795 |
PaxDbi | P69795 |
PRIDEi | P69795 |
PTM databases
iPTMneti | P69795 |
Expressioni
Inductioni
By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).1 Publication
Interactioni
Subunit structurei
Binary interactionsi
P69795
With | #Exp. | IntAct |
---|---|---|
chbA [P69791] | 2 | EBI-1128186,EBI-1121924 |
Protein-protein interaction databases
BioGRIDi | 4260316, 17 interactors |
ComplexPortali | CPX-4683, N,N'-diacetylchitobiose-specific enzyme II complex |
IntActi | P69795, 2 interactors |
STRINGi | 511145.b1738 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P69795 |
SMRi | P69795 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69795 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 106 | PTS EIIB type-3PROSITE-ProRule annotationAdd BLAST | 104 |
Domaini
The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | COG1440, Bacteria |
HOGENOMi | CLU_147323_2_0_6 |
InParanoidi | P69795 |
PhylomeDBi | P69795 |
Family and domain databases
InterProi | View protein in InterPro IPR036095, PTS_EIIB-like_sf IPR003501, PTS_EIIB_2/3 IPR013012, PTS_EIIB_3 |
Pfami | View protein in Pfam PF02302, PTS_IIB, 1 hit |
SUPFAMi | SSF52794, SSF52794, 1 hit |
TIGRFAMsi | TIGR00853, pts-lac, 1 hit |
PROSITEi | View protein in PROSITE PS51100, PTS_EIIB_TYPE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P69795-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKKHIYLFC SAGMSTSLLV SKMRAQAEKY EVPVIIEAFP ETLAGEKGQN
60 70 80 90 100
ADVVLLGPQI AYMLPEIQRL LPNKPVEVID SLLYGKVDGL GVLKAAVAAI
KKAAAN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52890 Genomic DNA Translation: CAA37069.1 U00096 Genomic DNA Translation: AAC74808.1 AP009048 Genomic DNA Translation: BAA15519.1 |
PIRi | S10870 |
RefSeqi | NP_416252.1, NC_000913.3 WP_000412169.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74808; AAC74808; b1738 BAA15519; BAA15519; BAA15519 |
GeneIDi | 52076592 945339 |
KEGGi | ecj:JW1727 eco:b1738 |
PATRICi | fig|1411691.4.peg.518 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52890 Genomic DNA Translation: CAA37069.1 U00096 Genomic DNA Translation: AAC74808.1 AP009048 Genomic DNA Translation: BAA15519.1 |
PIRi | S10870 |
RefSeqi | NP_416252.1, NC_000913.3 WP_000412169.1, NZ_STEB01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1E2B | NMR | - | A | 1-106 | [»] | |
1H9C | NMR | - | A | 1-106 | [»] | |
1IIB | X-ray | 1.80 | A/B | 1-106 | [»] | |
2WWV | NMR | - | D | 3-105 | [»] | |
2WY2 | NMR | - | D | 3-105 | [»] | |
BMRBi | P69795 | |||||
SMRi | P69795 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260316, 17 interactors |
ComplexPortali | CPX-4683, N,N'-diacetylchitobiose-specific enzyme II complex |
IntActi | P69795, 2 interactors |
STRINGi | 511145.b1738 |
Chemistry databases
DrugBanki | DB03544, S-Phosphocysteine |
Protein family/group databases
TCDBi | 4.A.3.2.1, the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family |
PTM databases
iPTMneti | P69795 |
Proteomic databases
jPOSTi | P69795 |
PaxDbi | P69795 |
PRIDEi | P69795 |
Genome annotation databases
EnsemblBacteriai | AAC74808; AAC74808; b1738 BAA15519; BAA15519; BAA15519 |
GeneIDi | 52076592 945339 |
KEGGi | ecj:JW1727 eco:b1738 |
PATRICi | fig|1411691.4.peg.518 |
Organism-specific databases
EchoBASEi | EB0138 |
Phylogenomic databases
eggNOGi | COG1440, Bacteria |
HOGENOMi | CLU_147323_2_0_6 |
InParanoidi | P69795 |
PhylomeDBi | P69795 |
Enzyme and pathway databases
BioCyci | EcoCyc:CELA-MONOMER MetaCyc:CELA-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P69795 |
PROi | PR:P69795 |
Family and domain databases
InterProi | View protein in InterPro IPR036095, PTS_EIIB-like_sf IPR003501, PTS_EIIB_2/3 IPR013012, PTS_EIIB_3 |
Pfami | View protein in Pfam PF02302, PTS_IIB, 1 hit |
SUPFAMi | SSF52794, SSF52794, 1 hit |
TIGRFAMsi | TIGR00853, pts-lac, 1 hit |
PROSITEi | View protein in PROSITE PS51100, PTS_EIIB_TYPE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTQB_ECOLI | |
Accessioni | P69795Primary (citable) accession number: P69795 Secondary accession number(s): P17409 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | May 10, 2005 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references