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Protein

PTS system N,N'-diacetylchitobiose-specific EIIB component

Gene

chbB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913117). Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117, PubMed:10913119).1 Publication2 Publications

Caution

Was originally (PubMed:2092358) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + N,N'-diacetylchitobiose(Side 1) = [protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate(Side 2).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei10Phosphocysteine intermediate5 Publications2 Publications1

GO - Molecular functioni

GO - Biological processi

  • N,N'-diacetylchitobiose import Source: EcoCyc
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CELA-MONOMER
MetaCyc:CELA-MONOMER

Protein family/group databases

TCDBi4.A.3.2.1 the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system N,N'-diacetylchitobiose-specific EIIB component1 Publication
Alternative name(s):
EIIB-Chb1 Publication
IVcel1 Publication
N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component1 Publication (EC:2.7.1.1962 Publications)
Gene namesi
Name:chbB1 Publication
Synonyms:celA1 Publication
Ordered Locus Names:b1738, JW1727
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10140 chbB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10C → S: Unable to be phosphorylated by EIIA. 2 Publications1

Chemistry databases

DrugBankiDB03544 S-Phosphocysteine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864891 – 106PTS system N,N'-diacetylchitobiose-specific EIIB componentAdd BLAST106

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10Phosphocysteine; by EIIAPROSITE-ProRule annotation1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69795
PRIDEiP69795

PTM databases

iPTMnetiP69795

Expressioni

Inductioni

By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).1 Publication

Interactioni

Subunit structurei

Forms a complex with ChbA (EIIA) (PubMed:10913119, PubMed:10913122, PubMed:19959833). ChbB is a monomer in both its unphosphorylated and phosphorylated forms (PubMed:10913122).3 Publications

Protein-protein interaction databases

BioGridi4260316, 17 interactors
IntActiP69795, 1 interactor
STRINGi316385.ECDH10B_1876

Structurei

Secondary structure

1106
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 11Combined sources8
Helixi13 – 29Combined sources17
Beta strandi34 – 40Combined sources7
Helixi41 – 43Combined sources3
Helixi44 – 48Combined sources5
Beta strandi52 – 56Combined sources5
Helixi58 – 63Combined sources6
Helixi64 – 70Combined sources7
Beta strandi71 – 74Combined sources4
Beta strandi76 – 78Combined sources3
Helixi81 – 85Combined sources5
Helixi89 – 104Combined sources16

3D structure databases

ProteinModelPortaliP69795
SMRiP69795
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69795

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 106PTS EIIB type-3PROSITE-ProRule annotationAdd BLAST104

Domaini

The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VZJ Bacteria
COG1440 LUCA
HOGENOMiHOG000224223
InParanoidiP69795
KOiK02760
OMAiVFMEKKH
PhylomeDBiP69795

Family and domain databases

InterProiView protein in InterPro
IPR036095 PTS_EIIB-like_sf
IPR003501 PTS_EIIB_2/3
IPR013012 PTS_EIIB_3
PfamiView protein in Pfam
PF02302 PTS_IIB, 1 hit
SUPFAMiSSF52794 SSF52794, 1 hit
TIGRFAMsiTIGR00853 pts-lac, 1 hit
PROSITEiView protein in PROSITE
PS51100 PTS_EIIB_TYPE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P69795-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKHIYLFC SAGMSTSLLV SKMRAQAEKY EVPVIIEAFP ETLAGEKGQN
60 70 80 90 100
ADVVLLGPQI AYMLPEIQRL LPNKPVEVID SLLYGKVDGL GVLKAAVAAI

KKAAAN
Length:106
Mass (Da):11,427
Last modified:May 10, 2005 - v1
Checksum:iDE4AADE300D18F53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA Translation: CAA37069.1
U00096 Genomic DNA Translation: AAC74808.1
AP009048 Genomic DNA Translation: BAA15519.1
PIRiS10870
RefSeqiNP_416252.1, NC_000913.3
WP_000412169.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74808; AAC74808; b1738
BAA15519; BAA15519; BAA15519
GeneIDi945339
KEGGiecj:JW1727
eco:b1738
PATRICifig|1411691.4.peg.518

Similar proteinsi

Entry informationi

Entry nameiPTQB_ECOLI
AccessioniPrimary (citable) accession number: P69795
Secondary accession number(s): P17409
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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