UniProtKB - P69791 (PTQA_ECOLI)
Protein
PTS system N,N'-diacetylchitobiose-specific EIIA component
Gene
chbA
Organism
Escherichia coli (strain K12)
Status
Functioni
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913118, PubMed:10913117). Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).1 Publication2 Publications
Caution
Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication
Cofactori
Mg2+1 Publication1 PublicationNote: Can also use copper and nickel with lower efficiency.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 89 | Tele-phosphohistidine intermediate4 Publications | 1 |
GO - Molecular functioni
- protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity Source: EcoCyc
GO - Biological processi
- N,N'-diacetylchitobiose import Source: EcoCyc
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc
Keywordsi
Molecular function | Transferase |
Biological process | Phosphotransferase system, Sugar transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:CELC-MONOMER MetaCyc:CELC-MONOMER |
Protein family/group databases
TCDBi | 4.A.3.2.1, the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family |
Names & Taxonomyi
Protein namesi | Recommended name: PTS system N,N'-diacetylchitobiose-specific EIIA component1 PublicationAlternative name(s): EIIA-Chb1 Publication EIII-Chb1 Publication IIIcel1 Publication N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component1 Publication |
Gene namesi | Name:chbA1 Publication Synonyms:celC1 Publication Ordered Locus Names:b1736, JW1725 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
- cytosol Source: EcoCyc
- protein-containing complex Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 92 | D → L: Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186494 | 1 – 116 | PTS system N,N'-diacetylchitobiose-specific EIIA componentAdd BLAST | 116 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 89 | Phosphohistidine; by HPrPROSITE-ProRule annotation3 Publications | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P69791 |
PRIDEi | P69791 |
PTM databases
iPTMneti | P69791 |
Expressioni
Inductioni
By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).1 Publication
Interactioni
Subunit structurei
Forms a complex with ChbB (EIIB) (PubMed:10913119, PubMed:10913122, PubMed:19959833). ChbA is a homotrimer (PubMed:10913122, PubMed:15654077, PubMed:19959833, PubMed:22593574). The interface of the homotrimer is stabilized by metal cations such as magnesium, copper or nickel (PubMed:15654077).
1 Publication5 PublicationsBinary interactionsi
P69791
With | #Exp. | IntAct |
---|---|---|
chbB [P69795] | 2 | EBI-1121924,EBI-1128186 |
Protein-protein interaction databases
BioGRIDi | 4259134, 12 interactors |
ComplexPortali | CPX-4683, N,N'-diacetylchitobiose-specific enzyme II complex |
DIPi | DIP-9265N |
IntActi | P69791, 12 interactors |
STRINGi | 511145.b1736 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P69791 |
SMRi | P69791 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69791 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 15 – 113 | PTS EIIA type-3PROSITE-ProRule annotationAdd BLAST | 99 |
Domaini
The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | COG1447, Bacteria |
HOGENOMi | CLU_152490_3_0_6 |
InParanoidi | P69791 |
PhylomeDBi | P69791 |
Family and domain databases
CDDi | cd00215, PTS_IIA_lac, 1 hit |
InterProi | View protein in InterPro IPR003188, PTS_IIA_lac/cel IPR036542, PTS_IIA_lac/cel_sf |
PANTHERi | PTHR34382, PTHR34382, 1 hit |
Pfami | View protein in Pfam PF02255, PTS_IIA, 1 hit |
PIRSFi | PIRSF000699, PTS_IILac_III, 1 hit |
SUPFAMi | SSF46973, SSF46973, 1 hit |
TIGRFAMsi | TIGR00823, EIIA-LAC, 1 hit |
PROSITEi | View protein in PROSITE PS51095, PTS_EIIA_TYPE_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P69791-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMDLDNIPDT QTEAEELEEV VMGLIINSGQ ARSLAYAALK QAKQGDFAAA
60 70 80 90 100
KAMMDQSRMA LNEAHLVQTK LIEGDAGEGK MKVSLVLVHA QDHLMTSMLA
110
RELITELIEL HEKLKA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 43 – 44 | KQ → NR in CAA37071 (PubMed:2179047).Curated | 2 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 14 | A → V in strain: ECOR 1. | 1 | |
Natural varianti | 52 | A → T in strain: ECOR 61. | 1 | |
Natural varianti | 59 | M → I in strain: ECOR 50. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52890 Genomic DNA Translation: CAA37071.1 M93570 Genomic DNA Translation: AAA23551.1 M93571 Genomic DNA Translation: AAA23553.1 M93572 Genomic DNA Translation: AAA23554.1 M93573 Genomic DNA Translation: AAA23559.1 M93574 Genomic DNA Translation: AAA23556.1 M93575 Genomic DNA Translation: AAA23552.1 M93576 Genomic DNA Translation: AAA23560.1 M93577 Genomic DNA Translation: AAA23557.1 M93591 Genomic DNA Translation: AAA23561.1 M93592 Genomic DNA Translation: AAA23555.1 M93593 Genomic DNA Translation: AAA23558.1 U00096 Genomic DNA Translation: AAC74806.1 AP009048 Genomic DNA Translation: BAA15517.1 |
PIRi | H64932 |
RefSeqi | NP_416250.1, NC_000913.3 WP_000968919.1, NZ_STEB01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC74806; AAC74806; b1736 BAA15517; BAA15517; BAA15517 |
GeneIDi | 52076594 946244 |
KEGGi | ecj:JW1725 eco:b1736 |
PATRICi | fig|1411691.4.peg.520 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52890 Genomic DNA Translation: CAA37071.1 M93570 Genomic DNA Translation: AAA23551.1 M93571 Genomic DNA Translation: AAA23553.1 M93572 Genomic DNA Translation: AAA23554.1 M93573 Genomic DNA Translation: AAA23559.1 M93574 Genomic DNA Translation: AAA23556.1 M93575 Genomic DNA Translation: AAA23552.1 M93576 Genomic DNA Translation: AAA23560.1 M93577 Genomic DNA Translation: AAA23557.1 M93591 Genomic DNA Translation: AAA23561.1 M93592 Genomic DNA Translation: AAA23555.1 M93593 Genomic DNA Translation: AAA23558.1 U00096 Genomic DNA Translation: AAC74806.1 AP009048 Genomic DNA Translation: BAA15517.1 |
PIRi | H64932 |
RefSeqi | NP_416250.1, NC_000913.3 WP_000968919.1, NZ_STEB01000009.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WCR | NMR | - | A/B/C | 14-116 | [»] | |
2LRK | NMR | - | A/B/C | 14-116 | [»] | |
2LRL | NMR | - | A/B/C | 14-116 | [»] | |
2WWV | NMR | - | A/B/C | 14-116 | [»] | |
2WY2 | NMR | - | A/B/C | 14-116 | [»] | |
BMRBi | P69791 | |||||
SMRi | P69791 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259134, 12 interactors |
ComplexPortali | CPX-4683, N,N'-diacetylchitobiose-specific enzyme II complex |
DIPi | DIP-9265N |
IntActi | P69791, 12 interactors |
STRINGi | 511145.b1736 |
Protein family/group databases
TCDBi | 4.A.3.2.1, the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family |
PTM databases
iPTMneti | P69791 |
Proteomic databases
PaxDbi | P69791 |
PRIDEi | P69791 |
Genome annotation databases
EnsemblBacteriai | AAC74806; AAC74806; b1736 BAA15517; BAA15517; BAA15517 |
GeneIDi | 52076594 946244 |
KEGGi | ecj:JW1725 eco:b1736 |
PATRICi | fig|1411691.4.peg.520 |
Organism-specific databases
EchoBASEi | EB0140 |
Phylogenomic databases
eggNOGi | COG1447, Bacteria |
HOGENOMi | CLU_152490_3_0_6 |
InParanoidi | P69791 |
PhylomeDBi | P69791 |
Enzyme and pathway databases
BioCyci | EcoCyc:CELC-MONOMER MetaCyc:CELC-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P69791 |
PROi | PR:P69791 |
Family and domain databases
CDDi | cd00215, PTS_IIA_lac, 1 hit |
InterProi | View protein in InterPro IPR003188, PTS_IIA_lac/cel IPR036542, PTS_IIA_lac/cel_sf |
PANTHERi | PTHR34382, PTHR34382, 1 hit |
Pfami | View protein in Pfam PF02255, PTS_IIA, 1 hit |
PIRSFi | PIRSF000699, PTS_IILac_III, 1 hit |
SUPFAMi | SSF46973, SSF46973, 1 hit |
TIGRFAMsi | TIGR00823, EIIA-LAC, 1 hit |
PROSITEi | View protein in PROSITE PS51095, PTS_EIIA_TYPE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PTQA_ECOLI | |
Accessioni | P69791Primary (citable) accession number: P69791 Secondary accession number(s): P17335 Q57128 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | May 10, 2005 | |
Last modified: | December 2, 2020 | |
This is version 120 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references