chbA

Functionⁱ

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913118, PubMed:10913117). Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).1 Publication2 Publications

Caution

Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

Cofactorⁱ

Mg²⁺1 Publication1 PublicationNote: Can also use copper and nickel with lower efficiency.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	89	Tele-phosphohistidine intermediate4 Publications		1

GO - Molecular functionⁱ

protein-phosphocysteine-N,N'-diacetylchitobiose phosphotransferase system transporter activity
Source: EcoCyc
Inferred from direct assayⁱ
- 10913117

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

N,N'-diacetylchitobiose import
Source: EcoCyc
Inferred from direct assayⁱ
- 10913117
phosphoenolpyruvate-dependent sugar phosphotransferase system
Source: EcoCyc
Inferred from direct assayⁱ
- 10913117
- 10913119
protein homooligomerization
Source: EcoCyc
Inferred from direct assayⁱ
- 10913122
- 15654077

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCycⁱ	EcoCyc:CELC-MONOMER MetaCyc:CELC-MONOMER

BioCycⁱ

EcoCyc:CELC-MONOMER
MetaCyc:CELC-MONOMER

Protein family/group databases

TCDBⁱ	4.A.3.2.1 the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family

TCDBⁱ

4.A.3.2.1 the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: PTS system N,N'-diacetylchitobiose-specific EIIA component1 Publication Alternative name(s): EIIA-Chb1 Publication EIII-Chb1 Publication IIIcel1 Publication N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component1 Publication
Gene namesⁱ	Name:chbA1 Publication Synonyms:celC1 Publication Ordered Locus Names:b1736, JW1725
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10142 chbA

EcoGeneⁱ

EG10142 chbA

Subcellular locationⁱ

Cytoplasm Curated

GO - Cellular componentⁱ

cytosol
Source: EcoCyc
Inferred from direct assayⁱ
- 10913122

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	92	D → L: Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000186494	1 – 116	PTS system N,N'-diacetylchitobiose-specific EIIA componentAdd BLAST		116

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	89	Phosphohistidine; by HPrPROSITE-ProRule annotation3 Publications		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	P69791
PRIDEⁱ	P69791

PTM databases

iPTMnetⁱ	P69791

iPTMnetⁱ

P69791

Expressionⁱ

Inductionⁱ

By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).1 Publication

Interactionⁱ

Subunit structureⁱ

Forms a complex with ChbB (EIIB) (PubMed:10913119, PubMed:10913122, PubMed:19959833). ChbA is a homotrimer (PubMed:10913122, PubMed:15654077, PubMed:19959833, PubMed:22593574). The interface of the homotrimer is stabilized by metal cations such as magnesium, copper or nickel (PubMed:15654077).1 Publication5 Publications

Protein-protein interaction databases

BioGridⁱ	4259134, 12 interactors
Database of interacting proteins More...DIPⁱ	DIP-9265N
IntActⁱ	P69791, 11 interactors
STRINGⁱ	316385.ECDH10B_1874

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	15 – 43	Combined sources	29
Helixⁱ	47 – 74	Combined sources	28
Beta strandⁱ	77 – 79	Combined sources	3
Helixⁱ	85 – 114	Combined sources	30

Show more details

3D structure databases

ProteinModelPortalⁱ	P69791
SMRⁱ	P69791
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P69791

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	15 – 113	PTS EIIA type-3PROSITE-ProRule annotationAdd BLAST		99

Domainⁱ

The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105VCP Bacteria COG1447 LUCA
HOGENOMⁱ	HOG000224338
InParanoidⁱ	P69791
KEGG Orthology (KO) More...KOⁱ	K02759
OMAⁱ	MGLIING
PhylomeDBⁱ	P69791

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00215 PTS_IIA_lac, 1 hit
InterProⁱ	View protein in InterPro IPR003188 PTS_IIA_lac/cel IPR036542 PTS_IIA_lac/cel_sf
PANTHERⁱ	PTHR34382 PTHR34382, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02255 PTS_IIA, 1 hit
PIRSFⁱ	PIRSF000699 PTS_IILac_III, 1 hit
SUPFAMⁱ	SSF46973 SSF46973, 1 hit
TIGRFAMsⁱ	TIGR00823 EIIA-LAC, 1 hit
PROSITEⁱ	View protein in PROSITE PS51095 PTS_EIIA_TYPE_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P69791-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MMDLDNIPDT QTEAEELEEV VMGLIINSGQ ARSLAYAALK QAKQGDFAAA 
        60         70         80         90        100
KAMMDQSRMA LNEAHLVQTK LIEGDAGEGK MKVSLVLVHA QDHLMTSMLA 
       110 
RELITELIEL HEKLKA

Length:116

Mass (Da):12,748

Last modified:May 10, 2005 - v1

Checksum:ⁱF2951DC6700FA8A9

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	43 – 44	KQ → NR in CAA37071 (PubMed:2179047).Curated		2

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱ	14	A → V in strain: ECOR 1.	1
Natural variantⁱ	52	A → T in strain: ECOR 61.	1
Natural variantⁱ	59	M → I in strain: ECOR 50.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X52890 Genomic DNA Translation: CAA37071.1 M93570 Genomic DNA Translation: AAA23551.1 M93571 Genomic DNA Translation: AAA23553.1 M93572 Genomic DNA Translation: AAA23554.1 M93573 Genomic DNA Translation: AAA23559.1 M93574 Genomic DNA Translation: AAA23556.1 M93575 Genomic DNA Translation: AAA23552.1 M93576 Genomic DNA Translation: AAA23560.1 M93577 Genomic DNA Translation: AAA23557.1 M93591 Genomic DNA Translation: AAA23561.1 M93592 Genomic DNA Translation: AAA23555.1 M93593 Genomic DNA Translation: AAA23558.1 U00096 Genomic DNA Translation: AAC74806.1 AP009048 Genomic DNA Translation: BAA15517.1
PIRⁱ	H64932
NCBI Reference Sequences More...RefSeqⁱ	NP_416250.1, NC_000913.3 WP_000968919.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74806; AAC74806; b1736 BAA15517; BAA15517; BAA15517
GeneIDⁱ	946244
KEGGⁱ	ecj:JW1725 eco:b1736
PATRICⁱ	fig\|1411691.4.peg.520

Protein	Similar proteins		Species	Score	Length	Source
P69791	PTS system N,N'-diacetylchitobiose-specific EIIA component		ECO57		116	UniRef100_P69793
PTS system N,N'-diacetylchitobiose-specific EIIA component		ECOL6		116
PTS system N,N'-diacetylchitobiose-specific EIIA component		SHIFL		116
PEP-dependent phosphotransferase enzyme III		SHIF8		151
N,N-diacetylchitobiose-specific phosphotransferase enzyme IIA component		SHIF2		151
+133

Protein	Similar proteins		Species	Score	Length	Source
P69791	PTS system N,N'-diacetylchitobiose-specific EIIA component		ECO57		116	UniRef90_P69793
PTS system N,N'-diacetylchitobiose-specific EIIA component		ECOL6		116
PTS system N,N'-diacetylchitobiose-specific EIIA component		SHIFL		116
PEP-dependent phosphotransferase enzyme III		SHIF8		151
N,N-diacetylchitobiose-specific phosphotransferase enzyme IIA component		SHIF2		151
+143

Protein	Similar proteins		Species	Score	Length	Source
P69791	PTS system N,N'-diacetylchitobiose-specific EIIA component		ECO57		116	UniRef50_P69793
PTS system N,N'-diacetylchitobiose-specific EIIA component		ECOL6		116
PTS system N,N'-diacetylchitobiose-specific EIIA component		SHIFL		116
PEP-dependent phosphotransferase enzyme III		SHIF8		151
N,N-diacetylchitobiose-specific phosphotransferase enzyme IIA component		SHIF2		151
+156

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X52890 Genomic DNA Translation: CAA37071.1 M93570 Genomic DNA Translation: AAA23551.1 M93571 Genomic DNA Translation: AAA23553.1 M93572 Genomic DNA Translation: AAA23554.1 M93573 Genomic DNA Translation: AAA23559.1 M93574 Genomic DNA Translation: AAA23556.1 M93575 Genomic DNA Translation: AAA23552.1 M93576 Genomic DNA Translation: AAA23560.1 M93577 Genomic DNA Translation: AAA23557.1 M93591 Genomic DNA Translation: AAA23561.1 M93592 Genomic DNA Translation: AAA23555.1 M93593 Genomic DNA Translation: AAA23558.1 U00096 Genomic DNA Translation: AAC74806.1 AP009048 Genomic DNA Translation: BAA15517.1
PIRⁱ	H64932
RefSeqⁱ	NP_416250.1, NC_000913.3 WP_000968919.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1WCR	NMR	-	A/B/C	14-116	[»]
	2LRK	NMR	-	A/B/C	14-116	[»]
	2LRL	NMR	-	A/B/C	14-116	[»]
	2WWV	NMR	-	A/B/C	14-116	[»]
	2WY2	NMR	-	A/B/C	14-116	[»]
ProteinModelPortalⁱ	P69791
SMRⁱ	P69791
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259134, 12 interactors
DIPⁱ	DIP-9265N
IntActⁱ	P69791, 11 interactors
STRINGⁱ	316385.ECDH10B_1874

Protein family/group databases

TCDBⁱ	4.A.3.2.1 the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family

TCDBⁱ

4.A.3.2.1 the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family

PTM databases

iPTMnetⁱ	P69791

iPTMnetⁱ

P69791

Proteomic databases

PaxDbⁱ	P69791
PRIDEⁱ	P69791

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74806; AAC74806; b1736 BAA15517; BAA15517; BAA15517
GeneIDⁱ	946244
KEGGⁱ	ecj:JW1725 eco:b1736
PATRICⁱ	fig\|1411691.4.peg.520

Organism-specific databases

EchoBASEⁱ	EB0140
EcoGeneⁱ	EG10142 chbA

Phylogenomic databases

eggNOGⁱ	ENOG4105VCP Bacteria COG1447 LUCA
HOGENOMⁱ	HOG000224338
InParanoidⁱ	P69791
KOⁱ	K02759
OMAⁱ	MGLIING
PhylomeDBⁱ	P69791

Enzyme and pathway databases

BioCycⁱ	EcoCyc:CELC-MONOMER MetaCyc:CELC-MONOMER

BioCycⁱ

EcoCyc:CELC-MONOMER
MetaCyc:CELC-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P69791
Protein Ontology More...PROⁱ	PR:P69791

Family and domain databases

CDDⁱ	cd00215 PTS_IIA_lac, 1 hit
InterProⁱ	View protein in InterPro IPR003188 PTS_IIA_lac/cel IPR036542 PTS_IIA_lac/cel_sf
PANTHERⁱ	PTHR34382 PTHR34382, 1 hit
Pfamⁱ	View protein in Pfam PF02255 PTS_IIA, 1 hit
PIRSFⁱ	PIRSF000699 PTS_IILac_III, 1 hit
SUPFAMⁱ	SSF46973 SSF46973, 1 hit
TIGRFAMsⁱ	TIGR00823 EIIA-LAC, 1 hit
PROSITEⁱ	View protein in PROSITE PS51095 PTS_EIIA_TYPE_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PTQA_ECOLI
Accessionⁱ	P69791Primary (citable) accession number: P69791 Secondary accession number(s): P17335 , Q47092, Q47093, Q47094, Q57128
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
	Last sequence update:	May 10, 2005
	Last modified:	March 28, 2018
	This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P69791 (PTQA_ECOLI)

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PTS system N,N'-diacetylchitobiose-specific EIIA component

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ