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Protein

PTS system N,N'-diacetylchitobiose-specific EIIA component

Gene

chbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II ChbABC PTS system is involved in the transport of the chitin disaccharide N,N'-diacetylchitobiose (GlcNAc2) (PubMed:10913118, PubMed:10913117). Also able to use N,N',N''-triacetyl chitotriose (GlcNAc3) (PubMed:10913117).1 Publication2 Publications

Caution

Was originally (PubMed:2179047) characterized as part of a cryptic cel operon for a cellobiose degradation system. The Cel+ phenotype is due to mutations making expression chitobiose-independent and altering the substrate specificity.1 Publication

Cofactori

Mg2+1 Publication1 PublicationNote: Can also use copper and nickel with lower efficiency.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Tele-phosphohistidine intermediate4 Publications1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:CELC-MONOMER
MetaCyc:CELC-MONOMER

Protein family/group databases

TCDBi4.A.3.2.1 the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system N,N'-diacetylchitobiose-specific EIIA component1 Publication
Alternative name(s):
EIIA-Chb1 Publication
EIII-Chb1 Publication
IIIcel1 Publication
N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component1 Publication
Gene namesi
Name:chbA1 Publication
Synonyms:celC1 Publication
Ordered Locus Names:b1736, JW1725
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10142 chbA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92D → L: Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864941 – 116PTS system N,N'-diacetylchitobiose-specific EIIA componentAdd BLAST116

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei89Phosphohistidine; by HPrPROSITE-ProRule annotation3 Publications1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69791
PRIDEiP69791

PTM databases

iPTMnetiP69791

Expressioni

Inductioni

By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-TCB).1 Publication

Interactioni

Subunit structurei

Forms a complex with ChbB (EIIB) (PubMed:10913119, PubMed:10913122, PubMed:19959833). ChbA is a homotrimer (PubMed:10913122, PubMed:15654077, PubMed:19959833, PubMed:22593574). The interface of the homotrimer is stabilized by metal cations such as magnesium, copper or nickel (PubMed:15654077).1 Publication5 Publications

Protein-protein interaction databases

BioGridi4259134, 12 interactors
DIPiDIP-9265N
IntActiP69791, 11 interactors
STRINGi316385.ECDH10B_1874

Structurei

Secondary structure

1116
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP69791
SMRiP69791
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69791

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 113PTS EIIA type-3PROSITE-ProRule annotationAdd BLAST99

Domaini

The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105VCP Bacteria
COG1447 LUCA
HOGENOMiHOG000224338
InParanoidiP69791
KOiK02759
OMAiMGLIING
PhylomeDBiP69791

Family and domain databases

CDDicd00215 PTS_IIA_lac, 1 hit
InterProiView protein in InterPro
IPR003188 PTS_IIA_lac/cel
IPR036542 PTS_IIA_lac/cel_sf
PANTHERiPTHR34382 PTHR34382, 1 hit
PfamiView protein in Pfam
PF02255 PTS_IIA, 1 hit
PIRSFiPIRSF000699 PTS_IILac_III, 1 hit
SUPFAMiSSF46973 SSF46973, 1 hit
TIGRFAMsiTIGR00823 EIIA-LAC, 1 hit
PROSITEiView protein in PROSITE
PS51095 PTS_EIIA_TYPE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P69791-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMDLDNIPDT QTEAEELEEV VMGLIINSGQ ARSLAYAALK QAKQGDFAAA
60 70 80 90 100
KAMMDQSRMA LNEAHLVQTK LIEGDAGEGK MKVSLVLVHA QDHLMTSMLA
110
RELITELIEL HEKLKA
Length:116
Mass (Da):12,748
Last modified:May 10, 2005 - v1
Checksum:iF2951DC6700FA8A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43 – 44KQ → NR in CAA37071 (PubMed:2179047).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti14A → V in strain: ECOR 1. 1
Natural varianti52A → T in strain: ECOR 61. 1
Natural varianti59M → I in strain: ECOR 50. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA Translation: CAA37071.1
M93570 Genomic DNA Translation: AAA23551.1
M93571 Genomic DNA Translation: AAA23553.1
M93572 Genomic DNA Translation: AAA23554.1
M93573 Genomic DNA Translation: AAA23559.1
M93574 Genomic DNA Translation: AAA23556.1
M93575 Genomic DNA Translation: AAA23552.1
M93576 Genomic DNA Translation: AAA23560.1
M93577 Genomic DNA Translation: AAA23557.1
M93591 Genomic DNA Translation: AAA23561.1
M93592 Genomic DNA Translation: AAA23555.1
M93593 Genomic DNA Translation: AAA23558.1
U00096 Genomic DNA Translation: AAC74806.1
AP009048 Genomic DNA Translation: BAA15517.1
PIRiH64932
RefSeqiNP_416250.1, NC_000913.3
WP_000968919.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74806; AAC74806; b1736
BAA15517; BAA15517; BAA15517
GeneIDi946244
KEGGiecj:JW1725
eco:b1736
PATRICifig|1411691.4.peg.520

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52890 Genomic DNA Translation: CAA37071.1
M93570 Genomic DNA Translation: AAA23551.1
M93571 Genomic DNA Translation: AAA23553.1
M93572 Genomic DNA Translation: AAA23554.1
M93573 Genomic DNA Translation: AAA23559.1
M93574 Genomic DNA Translation: AAA23556.1
M93575 Genomic DNA Translation: AAA23552.1
M93576 Genomic DNA Translation: AAA23560.1
M93577 Genomic DNA Translation: AAA23557.1
M93591 Genomic DNA Translation: AAA23561.1
M93592 Genomic DNA Translation: AAA23555.1
M93593 Genomic DNA Translation: AAA23558.1
U00096 Genomic DNA Translation: AAC74806.1
AP009048 Genomic DNA Translation: BAA15517.1
PIRiH64932
RefSeqiNP_416250.1, NC_000913.3
WP_000968919.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WCRNMR-A/B/C14-116[»]
2LRKNMR-A/B/C14-116[»]
2LRLNMR-A/B/C14-116[»]
2WWVNMR-A/B/C14-116[»]
2WY2NMR-A/B/C14-116[»]
ProteinModelPortaliP69791
SMRiP69791
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259134, 12 interactors
DIPiDIP-9265N
IntActiP69791, 11 interactors
STRINGi316385.ECDH10B_1874

Protein family/group databases

TCDBi4.A.3.2.1 the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family

PTM databases

iPTMnetiP69791

Proteomic databases

PaxDbiP69791
PRIDEiP69791

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74806; AAC74806; b1736
BAA15517; BAA15517; BAA15517
GeneIDi946244
KEGGiecj:JW1725
eco:b1736
PATRICifig|1411691.4.peg.520

Organism-specific databases

EchoBASEiEB0140
EcoGeneiEG10142 chbA

Phylogenomic databases

eggNOGiENOG4105VCP Bacteria
COG1447 LUCA
HOGENOMiHOG000224338
InParanoidiP69791
KOiK02759
OMAiMGLIING
PhylomeDBiP69791

Enzyme and pathway databases

BioCyciEcoCyc:CELC-MONOMER
MetaCyc:CELC-MONOMER

Miscellaneous databases

EvolutionaryTraceiP69791
PROiPR:P69791

Family and domain databases

CDDicd00215 PTS_IIA_lac, 1 hit
InterProiView protein in InterPro
IPR003188 PTS_IIA_lac/cel
IPR036542 PTS_IIA_lac/cel_sf
PANTHERiPTHR34382 PTHR34382, 1 hit
PfamiView protein in Pfam
PF02255 PTS_IIA, 1 hit
PIRSFiPIRSF000699 PTS_IILac_III, 1 hit
SUPFAMiSSF46973 SSF46973, 1 hit
TIGRFAMsiTIGR00823 EIIA-LAC, 1 hit
PROSITEiView protein in PROSITE
PS51095 PTS_EIIA_TYPE_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTQA_ECOLI
AccessioniPrimary (citable) accession number: P69791
Secondary accession number(s): P17335
, Q47092, Q47093, Q47094, Q57128
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: March 28, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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