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Protein

PTS system glucose-specific EIICB component

Gene

ptsG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of PtsG and Crr is involved in glucose transport (PubMed:3129430, PubMed:10562420). Also functions as a chemoreceptor monitoring the environment for changes in sugar concentration and an effector modulating the activity of the transcriptional repressor Mlc (PubMed:18319344).3 Publications

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2).1 Publication

Enzyme regulationi

Transporter activity may be inhibited by SgrT.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei421Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation3 Publications1

GO - Molecular functioni

GO - Biological processi

  • glucose transmembrane transport Source: CACAO
  • phosphoenolpyruvate-dependent sugar phosphotransferase system Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PTSG-MONOMER
MetaCyc:PTSG-MONOMER

Protein family/group databases

MoonProtiP69786
TCDBi4.A.1.1.1 the pts glucose-glucoside (glc) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucose-specific EIICB component1 Publication
Alternative name(s):
EIICB-Glc1 Publication
Short name:
EII-Glc1 Publication
Including the following 2 domains:
Glucose permease IIC component1 Publication
Alternative name(s):
PTS system glucose-specific EIIC component1 Publication
Glucose-specific phosphotransferase enzyme IIB component1 Publication (EC:2.7.1.1991 Publication)
Alternative name(s):
PTS system glucose-specific EIIB component1 Publication
Gene namesi
Name:ptsG
Synonyms:glcA, umg
Ordered Locus Names:b1101, JW1087
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10787 ptsG

Subcellular locationi

  • Cell inner membrane PROSITE-ProRule annotation1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicSequence analysisAdd BLAST14
Transmembranei15 – 35HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini36 – 50PeriplasmicSequence analysisAdd BLAST15
Transmembranei51 – 71HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini72 – 79CytoplasmicSequence analysis8
Transmembranei80 – 100HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini101 – 111PeriplasmicSequence analysisAdd BLAST11
Transmembranei112 – 132HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini133 – 151CytoplasmicSequence analysisAdd BLAST19
Transmembranei152 – 172HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini173 – 190PeriplasmicSequence analysisAdd BLAST18
Transmembranei191 – 211HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini212 – 249CytoplasmicSequence analysisAdd BLAST38
Transmembranei250 – 270HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini271 – 279PeriplasmicSequence analysis9
Transmembranei280 – 300HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini301 – 309CytoplasmicSequence analysis9
Transmembranei310 – 330HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini331 – 355PeriplasmicSequence analysisAdd BLAST25
Transmembranei356 – 376HelicalPROSITE-ProRule annotationAdd BLAST21
Topological domaini377 – 477CytoplasmicSequence analysisAdd BLAST101

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi204C → S: Destabilizes the protein structure; when associated with S-326. 1 Publication1
Mutagenesisi326C → S: Destabilizes the protein structure; when associated with S-204. 1 Publication1
Mutagenesisi421C → S: Unable to be phosphorylated and to catalyze the phosphoryl exchange between glucose and glucose 6-phosphate at equilibrium. 1 Publication1
Mutagenesisi449V → Q: Interaction with Mlc is hardly detectable. 1 Publication1
Mutagenesisi451A → F: The complex with mlc shows much weaker association than the wild-type. 1 Publication1
Mutagenesisi456Q → A: Interaction with Mlc is hardly detectable. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865281 – 477PTS system glucose-specific EIICB componentAdd BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei421Phosphocysteine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP69786
PaxDbiP69786
PRIDEiP69786

PTM databases

iPTMnetiP69786

Expressioni

Inductioni

The ptsG transcript is degraded under conditions of glucose-phosphate stress (due to intracellular accumulation of glucose-6-phosphate caused by disruption of glycolytic flux), or in the presence of (toxic) non-metabolizable glucose phosphate analogs due to hybridization with the small RNA sgrS (originally known as ryaA). This hybridization is sufficient to repress mRNA translation (PubMed:16549791). The hybrid is subsequently degraded by RNase E. This reduces the quantity of transporter and relieves glucose phosphate stress.2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mlcP504563EBI-903497,EBI-1116104

Protein-protein interaction databases

BioGridi4260940, 16 interactors
DIPiDIP-29833N
IntActiP69786, 3 interactors
STRINGi316385.ECDH10B_1173

Structurei

Secondary structure

1477
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi401 – 408Combined sources8
Helixi412 – 414Combined sources3
Beta strandi415 – 420Combined sources6
Beta strandi422 – 430Combined sources9
Helixi432 – 434Combined sources3
Helixi437 – 442Combined sources6
Beta strandi446 – 451Combined sources6
Beta strandi454 – 458Combined sources5
Helixi460 – 462Combined sources3
Helixi463 – 475Combined sources13

3D structure databases

ProteinModelPortaliP69786
SMRiP69786
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69786

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 388PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST388
Domaini399 – 477PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST79

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CI1 Bacteria
COG1263 LUCA
COG1264 LUCA
HOGENOMiHOG000250993
KOiK02778
K02779
OMAiAWAFNRF
PhylomeDBiP69786

Family and domain databases

CDDicd00212 PTS_IIB_glc, 1 hit
Gene3Di3.30.1360.60, 1 hit
InterProiView protein in InterPro
IPR036878 Glu_permease_IIB
IPR018113 PTrfase_EIIB_Cys
IPR003352 PTS_EIIC
IPR013013 PTS_EIIC_1
IPR001996 PTS_IIB_1
IPR011299 PTS_IIBC_glc
IPR004719 PTS_maltose/Glc_sub_IIC
PfamiView protein in Pfam
PF00367 PTS_EIIB, 1 hit
PF02378 PTS_EIIC, 1 hit
SUPFAMiSSF55604 SSF55604, 1 hit
TIGRFAMsiTIGR00826 EIIB_glc, 1 hit
TIGR00852 pts-Glc, 1 hit
TIGR02002 PTS-II-BC-glcB, 1 hit
PROSITEiView protein in PROSITE
PS51098 PTS_EIIB_TYPE_1, 1 hit
PS01035 PTS_EIIB_TYPE_1_CYS, 1 hit
PS51103 PTS_EIIC_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P69786-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKNAFANLQ KVGKSLMLPV SVLPIAGILL GVGSANFSWL PAVVSHVMAE
60 70 80 90 100
AGGSVFANMP LIFAIGVALG FTNNDGVSAL AAVVAYGIMV KTMAVVAPLV
110 120 130 140 150
LHLPAEEIAS KHLADTGVLG GIISGAIAAY MFNRFYRIKL PEYLGFFAGK
160 170 180 190 200
RFVPIISGLA AIFTGVVLSF IWPPIGSAIQ TFSQWAAYQN PVVAFGIYGF
210 220 230 240 250
IERCLVPFGL HHIWNVPFQM QIGEYTNAAG QVFHGDIPRY MAGDPTAGKL
260 270 280 290 300
SGGFLFKMYG LPAAAIAIWH SAKPENRAKV GGIMISAALT SFLTGITEPI
310 320 330 340 350
EFSFMFVAPI LYIIHAILAG LAFPICILLG MRDGTSFSHG LIDFIVLSGN
360 370 380 390 400
SSKLWLFPIV GIGYAIVYYT IFRVLIKALD LKTPGREDAT EDAKATGTSE
410 420 430 440 450
MAPALVAAFG GKENITNLDA CITRLRVSVA DVSKVDQAGL KKLGAAGVVV
460 470
AGSGVQAIFG TKSDNLKTEM DEYIRNH
Length:477
Mass (Da):50,677
Last modified:August 13, 1987 - v1
Checksum:iD97A80FD64B74F73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02618 Genomic DNA Translation: AAA24437.1
U00096 Genomic DNA Translation: AAC74185.1
AP009048 Genomic DNA Translation: BAA35908.1
PIRiA25336 WQEC2G
RefSeqiNP_415619.1, NC_000913.3
WP_000475719.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74185; AAC74185; b1101
BAA35908; BAA35908; BAA35908
GeneIDi945651
KEGGiecj:JW1087
eco:b1101
PATRICifig|1411691.4.peg.1167

Entry informationi

Entry nameiPTGCB_ECOLI
AccessioniPrimary (citable) accession number: P69786
Secondary accession number(s): P05053
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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