Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transient receptor potential cation channel subfamily V member 5

Gene

Trpv5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Constitutively active calcium selective cation channel thought to be involved in Ca2+ reabsorption in kidney and intestine (PubMed:12077127, PubMed:14679186). Required for normal Ca2+ reabsorption in the kidney distal convoluted tubules (PubMed:14679186). The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca2+-dependent feedback regulation includes fast channel inactivation and slow current decay (PubMed:12077127). Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating (By similarity).By similarity2 Publications

Activity regulationi

Activated by WNK3.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi535Calcium; shared with neighboring subunitsBy similarity1

GO - Molecular functioni

  • calcium channel activity Source: UniProtKB
  • calmodulin binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionCalcium channel, Calmodulin-binding, Ion channel
Biological processCalcium transport, Ion transport, Transport
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-3295583 TRP channels

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 5
Short name:
TrpV5
Alternative name(s):
Calcium transport protein 2
Short name:
CaT2
Epithelial calcium channel 1
Short name:
ECaC1
Osm-9-like TRP channel 3
Short name:
OTRPC3
Gene namesi
Name:Trpv5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2429764 Trpv5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 320CytoplasmicBy similarityAdd BLAST320
Transmembranei321 – 341HelicalBy similarityAdd BLAST21
Topological domaini342 – 378ExtracellularBy similarityAdd BLAST37
Transmembranei379 – 401HelicalBy similarityAdd BLAST23
Topological domaini402 – 412ExtracellularBy similarityAdd BLAST11
Transmembranei413 – 435HelicalBy similarityAdd BLAST23
Topological domaini436 – 441CytoplasmicBy similarity6
Transmembranei442 – 462HelicalBy similarityAdd BLAST21
Topological domaini463 – 485ExtracellularBy similarityAdd BLAST23
Transmembranei486 – 506HelicalBy similarityAdd BLAST21
Intramembranei517 – 537Pore-formingBy similarityAdd BLAST21
Transmembranei550 – 570HelicalBy similarityAdd BLAST21
Topological domaini571 – 723CytoplasmicBy similarityAdd BLAST153

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutant mice appear grossly normal and are fertile. They display polyuria and increased urinary excretion of Ca2+, due to defective Ca2+ reabsorption in the kidney distal convoluted tubules. Likewise, they display increased urinary excretion of phosphate. Besides, their urine has a lower pH. The polyuria and the urine acidification may be a response to the high urinary Ca2+ levels, preventing the formation of kidney stones (PubMed:14679186). Serum Ca2+ and phosphate levels are normal, probably due to increased expression of TRPV6 and increased Ca2+ absorption in the intestine. The increased expression of TRPV6 may be due to the increased serum levels of 1,25-dihydroxy-vitamin D3 that are observed in mutant mice (PubMed:14679186, PubMed:27102152). Age-related changes in trabecular and cortical bone mass are accelerated in male mutant mice, including reduced trabecular and cortical bone thickness. Still, this has no effect on bone strength (PubMed:27102152).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi403 – 406VGAS → LGVT: Induces faster Ca(2+)-dependent channel inactivation. 1 Publication4
Mutagenesisi593T → A: Abolishes interaction with S100A10, plasma membrane localization and channel activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153511 – 723Transient receptor potential cation channel subfamily V member 5Add BLAST723

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei678PhosphothreonineCombined sources1
Modified residuei682PhosphoserineCombined sources1

Post-translational modificationi

Glycosylated.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP69744
PRIDEiP69744

PTM databases

iPTMnetiP69744
PhosphoSitePlusiP69744

Expressioni

Tissue specificityi

Detected in kidney cortex (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000036899 Expressed in 22 organ(s), highest expression level in spinal cord
ExpressionAtlasiP69744 baseline and differential

Interactioni

Subunit structurei

Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6 (PubMed:12574114). Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane (PubMed:12660155). Interacts with calmodulin (PubMed:15123711). Interacts with BSPRY, which results in its inactivation (PubMed:16380433).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Wnk4Q80UE62EBI-538447,EBI-295378

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228781, 1 interactor
CORUMiP69744
IntActiP69744, 2 interactors
MINTiP69744
STRINGi10090.ENSMUSP00000031901

Structurei

3D structure databases

ProteinModelPortaliP69744
SMRiP69744
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati38 – 68ANK 1Sequence analysisAdd BLAST31
Repeati72 – 101ANK 2Sequence analysisAdd BLAST30
Repeati110 – 139ANK 3Sequence analysisAdd BLAST30
Repeati156 – 185ANK 4Sequence analysisAdd BLAST30
Repeati189 – 222ANK 5Sequence analysisAdd BLAST34
Repeati232 – 261ANK 6Sequence analysisAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni591 – 595Interaction with S100A101 Publication5
Regioni643 – 646Involved in Ca(2+)-dependent inactivationBy similarity4
Regioni693 – 723Involved in Ca(2+)-dependent inactivationBy similarityAdd BLAST31

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676 Eukaryota
ENOG4110DG4 LUCA
GeneTreeiENSGT00550000074425
HOGENOMiHOG000234397
HOVERGENiHBG061442
InParanoidiP69744
KOiK04974
OMAiYFGQTVL
OrthoDBiEOG091G0314
TreeFamiTF314711

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR005821 Ion_trans_dom
IPR004729 TRP_channel
IPR024862 TRPV
IPR008346 TRPV5
IPR008344 TRPV5/TRPV6
PANTHERiPTHR10582 PTHR10582, 1 hit
PTHR10582:SF11 PTHR10582:SF11, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00520 Ion_trans, 1 hit
PRINTSiPR01415 ANKYRIN
PR01765 ECACCHANNEL
PR01767 ECACCHANNEL2
SMARTiView protein in SMART
SM00248 ANK, 4 hits
SUPFAMiSSF48403 SSF48403, 1 hit
TIGRFAMsiTIGR00870 trp, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P69744-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGAKTPWIQL QKLLNWWVRD QDWNQHVDQL HMLQQKSIWE SPLLRAAKEN
60 70 80 90 100
DMCTLKKLQH DQNCDFRQRG ALGETALHVA ALYDNLDAAI MLMEAAPYLV
110 120 130 140 150
TESTLCEPFV GQTALHIAVM NQNVNLVRAL LARGASASAR ATGSAFHRSS
160 170 180 190 200
HNLIYYGEHP LSFAACVGSE EIVRLLIEHG ADIRAQDSLG NTVLHILVLQ
210 220 230 240 250
PNKTFACQMY NLLLSYDGGD HLKSLELVPN NQGLTPFKLA GVEGNTVMFQ
260 270 280 290 300
HLMQKRKRIQ WSFGPLTSSL YDLTEIDSWG EELSFLELVV SSKKKEARQI
310 320 330 340 350
LEQTPVKELV SLKWKKYGQP YFCLLGALYI FYMVCFTTCC VYRPLKFRDA
360 370 380 390 400
NRTHVRDNTI MEQKSLQEAY VTYQDKIRLV GELVTVIGAV IILLLEIPDI
410 420 430 440 450
FRVGASRYFG QTVLGGPFHV IIITYASLVL LTMAMRLTNV NGEVVPMSMA
460 470 480 490 500
LVLGWCSVMY FARGFQMLGP FTIMIQKMIF GDLLRFCWLM AMVILGFASA
510 520 530 540 550
FYIIFQTEDP DNLGEFSDYP TAMFSTFELF LTIIDGPANY RVDLPFMYSV
560 570 580 590 600
TYATFAIIAT LLMLNLFIAM MGDTHWRVAQ ERDELWRAQV VATTVMLERK
610 620 630 640 650
MPRFLWPRSG ICGCEYGLGD RWFLRVEHHQ EQNPYRVLRY VEAFKSSDKE
660 670 680 690 700
EVQEQLSEKQ PSGTETGTLA RGSVVLQTPP LSRTTSLSSN SHRGWEILRR
710 720
NTLGHLNLGL DPGEGDGEEI YQF
Length:723
Mass (Da):82,218
Last modified:July 27, 2011 - v2
Checksum:i6668C67C47CBB443
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YW69A0A0A6YW69_MOUSE
Transient receptor potential cation...
Trpv5
674Annotation score:

Sequence cautioni

The sequence AK085479 differs from that shown. Reason: Frameshift at position 579.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti302E → K in AK085479 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336378 Genomic DNA Translation: AAM53408.1
AK085479 mRNA No translation available.
BC110554 mRNA Translation: AAI10555.1
BC110555 mRNA Translation: AAI10556.1
CH466533 Genomic DNA Translation: EDL13503.1
CCDSiCCDS20053.1
RefSeqiNP_001007573.1, NM_001007572.2
XP_017176952.1, XM_017321463.1
UniGeneiMm.135734

Genome annotation databases

EnsembliENSMUST00000031901; ENSMUSP00000031901; ENSMUSG00000036899
GeneIDi194352
KEGGimmu:194352
UCSCiuc009bqd.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF336378 Genomic DNA Translation: AAM53408.1
AK085479 mRNA No translation available.
BC110554 mRNA Translation: AAI10555.1
BC110555 mRNA Translation: AAI10556.1
CH466533 Genomic DNA Translation: EDL13503.1
CCDSiCCDS20053.1
RefSeqiNP_001007573.1, NM_001007572.2
XP_017176952.1, XM_017321463.1
UniGeneiMm.135734

3D structure databases

ProteinModelPortaliP69744
SMRiP69744
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228781, 1 interactor
CORUMiP69744
IntActiP69744, 2 interactors
MINTiP69744
STRINGi10090.ENSMUSP00000031901

PTM databases

iPTMnetiP69744
PhosphoSitePlusiP69744

Proteomic databases

PaxDbiP69744
PRIDEiP69744

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031901; ENSMUSP00000031901; ENSMUSG00000036899
GeneIDi194352
KEGGimmu:194352
UCSCiuc009bqd.1 mouse

Organism-specific databases

CTDi56302
MGIiMGI:2429764 Trpv5

Phylogenomic databases

eggNOGiKOG3676 Eukaryota
ENOG4110DG4 LUCA
GeneTreeiENSGT00550000074425
HOGENOMiHOG000234397
HOVERGENiHBG061442
InParanoidiP69744
KOiK04974
OMAiYFGQTVL
OrthoDBiEOG091G0314
TreeFamiTF314711

Enzyme and pathway databases

ReactomeiR-MMU-3295583 TRP channels

Miscellaneous databases

PROiPR:P69744
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036899 Expressed in 22 organ(s), highest expression level in spinal cord
ExpressionAtlasiP69744 baseline and differential

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR005821 Ion_trans_dom
IPR004729 TRP_channel
IPR024862 TRPV
IPR008346 TRPV5
IPR008344 TRPV5/TRPV6
PANTHERiPTHR10582 PTHR10582, 1 hit
PTHR10582:SF11 PTHR10582:SF11, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 1 hit
PF00520 Ion_trans, 1 hit
PRINTSiPR01415 ANKYRIN
PR01765 ECACCHANNEL
PR01767 ECACCHANNEL2
SMARTiView protein in SMART
SM00248 ANK, 4 hits
SUPFAMiSSF48403 SSF48403, 1 hit
TIGRFAMsiTIGR00870 trp, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTRPV5_MOUSE
AccessioniPrimary (citable) accession number: P69744
Secondary accession number(s): Q2TB50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again