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Protein

Gag polyprotein

Gene

gag

Organism
Equine infectious anemia virus (strain Wyoming) (EIAV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Matrix protein p15 forms the outer shell of the core of the virus, lining the inner surface of the viral membrane.By similarity
Capsid protein p26 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p11 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).By similarity
p9 plays a role in budding of the assembled particle by interacting with PDCD6IP/AIP1.By similarity

Caution

The original EMBL accession numbers (M11337 and M14855) assigned to this isolate (isolate Wyoming) have been made secondary to M16575 which is from a different isolate (clone 1365).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri381 – 398CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri400 – 417CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionViral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Cleaved into the following 5 chains:
Gene namesi
Name:gag
OrganismiEquine infectious anemia virus (strain Wyoming) (EIAV)
Taxonomic identifieri11672 [NCBI]
Taxonomic lineageiVirusesOrterviralesRetroviridaeOrthoretrovirinaeLentivirus
Virus hostiEquus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000387801 – 124Matrix protein p15Add BLAST124
ChainiPRO_0000038781125 – 354Capsid protein p26Add BLAST230
PeptideiPRO_0000272315355 – 359p1Sequence analysis5
ChainiPRO_0000038782360 – 435Nucleocapsid protein p11Add BLAST76
ChainiPRO_0000038783436 – 486p9Add BLAST51

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi322 ↔ 342

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP69732

Interactioni

Subunit structurei

p9 interacts with human PDCD6IP/AIP1.By similarity

Protein-protein interaction databases

ELMiP69732

Structurei

Secondary structure

1486
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP69732
SMRiP69732
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69732

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi457 – 461LYPX(n)L motif5

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. p9 contains one L domain: a LYPX(n)L motif, which interacts with PDCD6IP/AIP1 (By similarity).By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri381 – 398CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri400 – 417CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

Gene3Di1.10.1200.30, 1 hit
1.10.150.90, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR014834 Gag_p15
IPR000721 Gag_p24
IPR012344 Matrix_HIV/RSV_N
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF08723 Gag_p15, 1 hit
PF00607 Gag_p24, 1 hit
PF00098 zf-CCHC, 2 hits
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69732-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDPLTWSKA LKKLEKVTVQ GSQKLTTGNC NWALSLVDLF HDTNFVKEKD
60 70 80 90 100
WQLRDVIPLL EDVTQTLSGQ EREAFERTWW AISAVKMGLQ INNVVDGKAS
110 120 130 140 150
FQLLRAKYEK KTANKKQSEP SEEYPIMIDG AGNRNFRPLT PRGYTTWVNT
160 170 180 190 200
IQTNGLLNEA SQNLFGILSV DCTSEEMNAF LDVVPGQAGQ KQILLDAIDK
210 220 230 240 250
IADDWDNRHP LPNAPLVAPP QGPIPMTARF IRGLGVPRER QMEPAFDQFR
260 270 280 290 300
QTYRQWIIEA MSEGIKVMIG KPKAQNIRQG AKEPYPEFVD RLLSQIKSEG
310 320 330 340 350
HPQEISKFLT DTLTIQNANE ECRNAMRHLR PEDTLEEKMY ACRDIGTTKQ
360 370 380 390 400
KMMLLAKALQ TGLAGPFKGG ALKGGPLKAA QTCYNCGKPG HLSSQCRAPK
410 420 430 440 450
VCFKCKQPGH FSKQCRSVPK NGKQGAQGRP QKQTFPIQQK SQHNKSVVQE
460 470 480
TPQTQNLYPD LSEIKKEYNV KEKDQVEDLN LDSLWE
Length:486
Mass (Da):54,809
Last modified:July 21, 1986 - v1
Checksum:i97137FA5933D1DDA
GO

Sequence databases

PIRiA03949 FOLJEV

Similar proteinsi

Cross-referencesi

Sequence databases

PIRiA03949 FOLJEV

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EIAX-ray2.70A140-346[»]
1HEKX-ray2.80A/B1-124[»]
2BL6NMR-A381-417[»]
2EIAX-ray2.70A/B141-346[»]
2K84NMR-A457-486[»]
2R03X-ray2.59B456-463[»]
4ZUTX-ray2.60C21-32[»]
4ZUUX-ray2.20C172-180[»]
4ZUWX-ray2.60C21-32[»]
ProteinModelPortaliP69732
SMRiP69732
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ELMiP69732

Proteomic databases

PRIDEiP69732

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP69732

Family and domain databases

Gene3Di1.10.1200.30, 1 hit
1.10.150.90, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR014834 Gag_p15
IPR000721 Gag_p24
IPR012344 Matrix_HIV/RSV_N
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF08723 Gag_p15, 1 hit
PF00607 Gag_p24, 1 hit
PF00098 zf-CCHC, 2 hits
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiGAG_EIAVY
AccessioniPrimary (citable) accession number: P69732
Secondary accession number(s): P03351
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 20, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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