UniProtKB - P69697 (TAT_HV1B1)
Protein Tat
tat
Functioni
Miscellaneous
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 11 | Essential for Tat translocation through the endosomal membraneUniRule annotation | 1 | |
Metal bindingi | 22 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 25 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 27 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 30 | Zinc 2UniRule annotation | 1 | |
Metal bindingi | 33 | Zinc 1; via pros nitrogenUniRule annotation | 1 | |
Metal bindingi | 34 | Zinc 1UniRule annotation | 1 | |
Metal bindingi | 37 | Zinc 1UniRule annotation | 1 |
GO - Molecular functioni
- actinin binding Source: UniProtKB-UniRule
- cyclin binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-UniRule
- protein domain specific binding Source: UniProtKB-UniRule
- RNA-binding transcription regulator activity Source: UniProtKB-UniRule
- trans-activation response element binding Source: UniProtKB-UniRule
GO - Biological processi
- modulation by virus of host chromatin organization Source: UniProtKB-UniRule
- modulation by virus of host PP1 activity Source: UniProtKB-UniRule
- negative regulation of peptidyl-threonine phosphorylation Source: UniProtKB-UniRule
- positive regulation of transcription elongation from RNA polymerase II promoter Source: UniProtKB-UniRule
- positive regulation of viral transcription Source: UniProtKB-UniRule
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-UniRule
- transcription, DNA-templated Source: UniProtKB-UniRule
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Protein TatUniRule annotationAlternative name(s): Transactivating regulatory proteinUniRule annotation |
Gene namesi | Name:tatUniRule annotation |
Organismi | Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1) |
Taxonomic identifieri | 11678 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Pararnavirae › Artverviricota › Revtraviricetes › Ortervirales › Retroviridae › Orthoretrovirinae › Lentivirus › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- host nucleolus UniRule annotation
- Host cytoplasm UniRule annotation
- Secreted UniRule annotation Note: Probably localizes to both nuclear and nucleolar compartments. Nuclear localization is mediated through the interaction of the nuclear localization signal with importin KPNB1. Secretion occurs through a Golgi-independent pathway. Tat is released from infected cells to the extracellular space where it remains associated to the cell membrane, or is secreted into the cerebrospinal fluid and sera. Extracellular Tat can be endocytosed by surrounding uninfected cells via binding to several receptors depending on the cell type.UniRule annotation
Keywords - Cellular componenti
Host cytoplasm, Host nucleus, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 11 | W → A: Unable to insert into membranes upon acidification. 40% loss of transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 11 | W → F: 85% loss of transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 11 | W → L: Unable to insert into membranes upon acidification. 95% loss of transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 11 | W → Y: When added outside the cell, hardly reaches the cytosol. 60% loss of transactivation activity. 1 Publication | 1 | |
Mutagenesisi | 52 | R → K: Decreases methylation by host PRMT6; almost abolishes methylation by host PRMT6; when associated with K-53. 1 Publication | 1 | |
Mutagenesisi | 53 | R → K: Decreases methylation by host PRMT6; almost abolishes methylation by host PRMT6; when associated with K-52. 1 Publication | 1 |
Keywords - Diseasei
AIDSPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085337 | 1 – 86 | Protein TatAdd BLAST | 86 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 28 | N6-acetyllysine; by host PCAFUniRule annotation | 1 | |
Modified residuei | 50 | N6-acetyllysine; by host EP300 and GCN5L2UniRule annotation | 1 | |
Modified residuei | 51 | N6-acetyllysine; by host EP300 and GCN5L2UniRule annotation | 1 | |
Modified residuei | 52 | Asymmetric dimethylarginine; by host PRMT6UniRule annotation1 Publication | 1 | |
Modified residuei | 53 | Asymmetric dimethylarginine; by host PRMT6UniRule annotation2 Publications | 1 | |
Cross-linki | 71 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)UniRule annotation |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationPTM databases
iPTMneti | P69697 |
SwissPalmi | P69697 |
Interactioni
Subunit structurei
Interacts with host CCNT1. Associates with the P-TEFb complex composed at least of Tat, P-TEFb (CDK9 and CCNT1), TAR RNA, RNA Pol II. Recruits the HATs CREBBP, TAF1/TFIID, EP300, PCAF and GCN5L2.
Interacts with host KAT5/Tip60; this interaction targets the latter to degradation.
Interacts with the host deacetylase SIRT1.
Interacts with host capping enzyme RNGTT; this interaction stimulates RNGTT. Binds to host KDR, and to the host integrins ITGAV/ITGB3 and ITGA5/ITGB1.
Interacts with host KPNB1/importin beta-1 without previous binding to KPNA1/importin alpha-1.
Interacts with EIF2AK2.
Interacts with host nucleosome assembly protein NAP1L1; this interaction may be required for the transport of Tat within the nucleus, since the two proteins interact at the nuclear rim.
Interacts with host C1QBP/SF2P32; this interaction involves lysine-acetylated Tat.
Interacts with the host chemokine receptors CCR2, CCR3 and CXCR4.
Interacts with host DPP4/CD26; this interaction may trigger an anti-proliferative effect.
Interacts with host LDLR.
Interacts with the host extracellular matrix metalloproteinase MMP1.
Interacts with host PRMT6; this interaction mediates Tat's methylation.
Interacts with, and is ubiquitinated by MDM2/Hdm2.
Interacts with host PSMC3 and HTATIP2.
Interacts with STAB1; this interaction may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T cells by binding to the same domain than HDAC1.
Interacts (when acetylated) with human CDK13, thereby increasing HIV-1 mRNA splicing and promoting the production of the doubly spliced HIV-1 protein Nef.
Interacts with host TBP; this interaction modulates the activity of transcriptional pre-initiation complex.
Interacts with host RELA.
UniRule annotationGO - Molecular functioni
- actinin binding Source: UniProtKB-UniRule
- cyclin binding Source: UniProtKB-UniRule
- protein domain specific binding Source: UniProtKB-UniRule
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 48 | TransactivationUniRule annotationAdd BLAST | 48 | |
Regioni | 1 – 24 | Interaction with human CREBBPUniRule annotationAdd BLAST | 24 | |
Regioni | 22 – 37 | Cysteine-richUniRule annotationAdd BLAST | 16 | |
Regioni | 38 – 48 | CoreUniRule annotationAdd BLAST | 11 | |
Regioni | 49 – 86 | Interaction with the host capping enzyme RNGTTUniRule annotationAdd BLAST | 38 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 49 – 57 | Nuclear localization signal, RNA-binding (TAR), and protein transductionUniRule annotation | 9 | |
Motifi | 78 – 80 | Cell attachment siteUniRule annotation | 3 |
Domaini
Sequence similaritiesi
Family and domain databases
Gene3Di | 4.10.20.10, 1 hit |
HAMAPi | MF_04079, HIV_TAT, 1 hit |
InterProi | View protein in InterPro IPR001831, IV_Tat IPR036963, Tat_dom_sf |
Pfami | View protein in Pfam PF00539, Tat, 1 hit |
PRINTSi | PR00055, HIVTATDOMAIN |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEPVDPRLEP WKHPGSQPKT ACTNCYCKKC CFHCQVCFIT KALGISYGRK
60 70 80
KRRQRRRPPQ GSQTHQVSLS KQPTSQSRGD PTGPKE
The sequence of this isoform differs from the canonical sequence as follows:
73-86: Missing.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 77 | S → P in strain: Isolate PV22. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_022298 | 73 – 86 | Missing in isoform Short. Add BLAST | 14 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15654 Genomic RNA Translation: AAA44199.1 K02083 Genomic DNA Translation: AAB59870.1 X01762 Genomic RNA No translation available. |
PIRi | S33982 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15654 Genomic RNA Translation: AAA44199.1 K02083 Genomic DNA Translation: AAB59870.1 X01762 Genomic RNA No translation available. |
PIRi | S33982 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5L1Z | X-ray | 5.90 | D | 1-57 | [»] | |
SMRi | P69697 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
PTM databases
iPTMneti | P69697 |
SwissPalmi | P69697 |
Family and domain databases
Gene3Di | 4.10.20.10, 1 hit |
HAMAPi | MF_04079, HIV_TAT, 1 hit |
InterProi | View protein in InterPro IPR001831, IV_Tat IPR036963, Tat_dom_sf |
Pfami | View protein in Pfam PF00539, Tat, 1 hit |
PRINTSi | PR00055, HIVTATDOMAIN |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TAT_HV1B1 | |
Accessioni | P69697Primary (citable) accession number: P69697 Secondary accession number(s): P04606, P04607 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | April 7, 2021 | |
This is version 97 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families