UniProtKB - P69681 (AMTB_ECOLI)

BLAST

>sp|P69681|AMTB_ECOLI Ammonia channel OS=Escherichia coli (strain K12) OX=83333 GN=amtB PE=1 SV=1
MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGLIRG
KNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINWLMLKNIELTAVMGSIYQY
IHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTLSYIPIAHMVWGGGLLASHGA
LDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPHNLPMVFTGTAILYIGWFGFNAG
SAGTANEIAALAFVNTVVATAAAILGWIFGEWALRGKPSLLGACSGAIAGLVGVTPACGY
IGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPCDVFGVHGVCGIVGCIMTGIFAASSLG
GVGFAEGVTMGHQLLVQLESIAITIVWSGVVAFIGYKLADLTVGLRVPEEQEREGLDVNS
HGENAYNA

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History
Entry version 115 (07 Nov 2018)
Sequence version 1 (29 Mar 2005)
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Protein

Ammonia channel

Gene

amtB

Organism

Escherichia coli (strain K12)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Involved in the uptake of ammonia.

GO - Molecular functionⁱ

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ammonium transport
Source: UniProtKB
Inferred from direct assayⁱ
- 19273840
carbon dioxide transport
Source: UniProtKB
Inferred from direct assayⁱ
- 19273840

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Ammonia transport, Transport

Biological process

Ammonia transport, Transport

Enzyme and pathway databases

BioCycⁱ	EcoCyc:AMTB-MONOMER MetaCyc:AMTB-MONOMER

BioCycⁱ

EcoCyc:AMTB-MONOMER
MetaCyc:AMTB-MONOMER

Protein family/group databases

TCDBⁱ	1.A.11.1.1 the ammonium transporter channel (amt) family

TCDBⁱ

1.A.11.1.1 the ammonium transporter channel (amt) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ammonia channel Alternative name(s): Ammonia transporter
Gene namesⁱ	Name:amtB Synonyms:ybaG Ordered Locus Names:b0451, JW0441
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11821 amtB

EcoGeneⁱ

EG11821 amtB

Subcellular locationⁱ

Cell inner membrane ; Multi-pass membrane protein

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	23 – 28	Periplasmic	6
Transmembraneⁱ	29 – 55	Helical; Name=1Add BLAST	27
Topological domainⁱ	56 – 59	Cytoplasmic	4
Transmembraneⁱ	60 – 90	Helical; Name=2Add BLAST	31
Topological domainⁱ	91 – 118	PeriplasmicAdd BLAST	28
Transmembraneⁱ	119 – 141	Helical; Name=3Add BLAST	23
Topological domainⁱ	142 – 146	Cytoplasmic	5
Transmembraneⁱ	147 – 170	Helical; Name=4Add BLAST	24
Topological domainⁱ	171 – 184	PeriplasmicAdd BLAST	14
Transmembraneⁱ	185 – 203	Helical; Name=5Add BLAST	19
Topological domainⁱ	204 – 215	CytoplasmicAdd BLAST	12
Transmembraneⁱ	216 – 242	Helical; Name=6Add BLAST	27
Topological domainⁱ	243 – 245	Periplasmic	3
Transmembraneⁱ	246 – 275	Helical; Name=7Add BLAST	30
Topological domainⁱ	276 – 279	Cytoplasmic	4
Transmembraneⁱ	280 – 294	Helical; Name=8Add BLAST	15
Topological domainⁱ	295 – 302	Periplasmic	8
Transmembraneⁱ	303 – 328	Helical; Name=9Add BLAST	26
Topological domainⁱ	329 – 332	Cytoplasmic	4
Transmembraneⁱ	333 – 354	Helical; Name=10Add BLAST	22
Topological domainⁱ	355 – 369	PeriplasmicAdd BLAST	15
Transmembraneⁱ	370 – 402	Helical; Name=11Add BLAST	33
Topological domainⁱ	403 – 428	CytoplasmicAdd BLAST	26

GO - Cellular componentⁱ

integral component of plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 16630265
plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 15919996
- 17309111

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB04147 Lauryl Dimethylamine-N-Oxide DB01828 Methylamine

DrugBankⁱ

DB04147 Lauryl Dimethylamine-N-Oxide
DB01828 Methylamine

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 22	1 PublicationAdd BLAST		22
ChainⁱPRO_0000001307	23 – 428	Ammonia channelAdd BLAST		406

Proteomic databases

PaxDbⁱ	P69681
PRIDEⁱ	P69681

Interactionⁱ

Subunit structureⁱ

Homotrimer. May interact with GlnK.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		4	EBI-9137905,EBI-9137905
glnK	P0AC55	3	EBI-9137905,EBI-559503

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 18362341
- 24899312

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4259856, 9 interactors
Database of interacting proteins More...DIPⁱ	DIP-29874N
IntActⁱ	P69681, 3 interactors
STRINGⁱ	316385.ECDH10B_0407

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	29 – 46	Combined sources	18
Helixⁱ	49 – 55	Combined sources	7
Helixⁱ	60 – 83	Combined sources	24
Helixⁱ	85 – 90	Combined sources	6
Beta strandⁱ	94 – 96	Combined sources	3
Beta strandⁱ	100 – 102	Combined sources	3
Helixⁱ	103 – 105	Combined sources	3
Helixⁱ	119 – 141	Combined sources	23
Helixⁱ	142 – 144	Combined sources	3
Helixⁱ	147 – 160	Combined sources	14
Helixⁱ	162 – 170	Combined sources	9
Beta strandⁱ	171 – 173	Combined sources	3
Helixⁱ	174 – 178	Combined sources	5
Turnⁱ	186 – 189	Combined sources	4
Helixⁱ	190 – 203	Combined sources	14
Turnⁱ	208 – 212	Combined sources	5
Helixⁱ	217 – 219	Combined sources	3
Helixⁱ	220 – 239	Combined sources	20
Helixⁱ	240 – 242	Combined sources	3
Beta strandⁱ	243 – 246	Combined sources	4
Helixⁱ	247 – 275	Combined sources	29
Helixⁱ	280 – 294	Combined sources	15
Turnⁱ	295 – 300	Combined sources	6
Helixⁱ	303 – 328	Combined sources	26
Helixⁱ	333 – 335	Combined sources	3
Helixⁱ	336 – 354	Combined sources	19
Helixⁱ	357 – 359	Combined sources	3
Helixⁱ	370 – 402	Combined sources	33
Helixⁱ	409 – 414	Combined sources	6
Helixⁱ	416 – 421	Combined sources	6

Show more details

3D structure databases

ProteinModelPortalⁱ	P69681
SMRⁱ	P69681
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P69681

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ammonia transporter channel (TC 1.A.11.2) family. [View classification]Curated

Keywords - Domainⁱ

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4105BZU Bacteria ENOG410XNMH LUCA
HOGENOMⁱ	HOG000017736
InParanoidⁱ	P69681
KEGG Orthology (KO) More...KOⁱ	K03320
PhylomeDBⁱ	P69681

Family and domain databases

Gene3Dⁱ	1.10.3430.10, 1 hit
InterProⁱ	View protein in InterPro IPR029020 Ammonium/urea_transptr IPR001905 Ammonium_transpt IPR018047 Ammonium_transpt_CS IPR024041 NH4_transpt_AmtB-like_dom
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00909 Ammonium_transp, 1 hit
TIGRFAMsⁱ	TIGR00836 amt, 1 hit
PROSITEⁱ	View protein in PROSITE PS01219 AMMONIUM_TRANSP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P69681-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKIATIKTGL ASLAMLPGLV MAAPAVADKA DNAFMMICTA LVLFMTIPGI 
        60         70         80         90        100
ALFYGGLIRG KNVLSMLTQV TVTFALVCIL WVVYGYSLAF GEGNNFFGNI 
       110        120        130        140        150
NWLMLKNIEL TAVMGSIYQY IHVAFQGSFA CITVGLIVGA LAERIRFSAV 
       160        170        180        190        200
LIFVVVWLTL SYIPIAHMVW GGGLLASHGA LDFAGGTVVH INAAIAGLVG 
       210        220        230        240        250
AYLIGKRVGF GKEAFKPHNL PMVFTGTAIL YIGWFGFNAG SAGTANEIAA 
       260        270        280        290        300
LAFVNTVVAT AAAILGWIFG EWALRGKPSL LGACSGAIAG LVGVTPACGY 
       310        320        330        340        350
IGVGGALIIG VVAGLAGLWG VTMLKRLLRV DDPCDVFGVH GVCGIVGCIM 
       360        370        380        390        400
TGIFAASSLG GVGFAEGVTM GHQLLVQLES IAITIVWSGV VAFIGYKLAD 
       410        420 
LTVGLRVPEE QEREGLDVNS HGENAYNA

Length:428

Mass (Da):44,515

Last modified:March 29, 2005 - v1

Checksum:ⁱB4AC96F0E5AE2B59

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	377 – 382	QLESIA → SWKASP in M63308 (PubMed:1645722).Curated		6

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U40429 Genomic DNA Translation: AAD14837.1 U82664 Genomic DNA Translation: AAB40207.1 U00096 Genomic DNA Translation: AAC73554.1 AP009048 Genomic DNA Translation: BAE76231.1 M63308 Genomic DNA No translation available.
PIRⁱ	A90692 C64775 E85542
NCBI Reference Sequences More...RefSeqⁱ	NP_414985.1, NC_000913.3 WP_000685029.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73554; AAC73554; b0451 BAE76231; BAE76231; BAE76231
GeneIDⁱ	945084
KEGGⁱ	ecj:JW0441 eco:b0451
PATRICⁱ	fig\|1411691.4.peg.1824

Protein	Similar proteins		Species	Score	Length	Source
P69681	Ammonia channel	)	ECO57		428	UniRef100_P69680
Ammonia channel		Shigella sp.		428
Ammonium transporter		Escherichia coli M8		428
Ammonium transporter		SHIBO		428
Ammonium transporter		ECOLX		428
+123

Protein	Similar proteins		Species	Score	Length	Source
P69681	Ammonia channel	)	ECO57		428	UniRef90_P69680
Ammonia channel		Shigella sp.		428
Ammonium transporter		Escherichia coli M8		428
Ammonium transporter		SHIBO		428
Ammonium transporter		ECOLX		428
+1055

Protein	Similar proteins		Species	Score	Length	Source
P69681	Ammonia channel	)	ECO57		428	UniRef50_P69680
Ammonia channel		Shigella sp.		428
Ammonium transporter		Escherichia coli M8		428
Ammonium transporter		SHIBO		428
Ammonium transporter		ECOLX		428
+4006

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U40429 Genomic DNA Translation: AAD14837.1 U82664 Genomic DNA Translation: AAB40207.1 U00096 Genomic DNA Translation: AAC73554.1 AP009048 Genomic DNA Translation: BAE76231.1 M63308 Genomic DNA No translation available.
PIRⁱ	A90692 C64775 E85542
RefSeqⁱ	NP_414985.1, NC_000913.3 WP_000685029.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1U77	X-ray	2.00	A	23-407	[»]
	1U7C	X-ray	1.85	A	23-407	[»]
	1U7G	X-ray	1.40	A	23-407	[»]
	1XQE	X-ray	2.10	A	23-428	[»]
	1XQF	X-ray	1.80	A	23-428	[»]
	2NMR	X-ray	2.10	A	23-428	[»]
	2NOP	X-ray	2.00	A	23-428	[»]
	2NOW	X-ray	2.20	A	23-428	[»]
	2NPC	X-ray	2.10	A	23-428	[»]
	2NPD	X-ray	2.10	A	23-428	[»]
	2NPE	X-ray	2.10	A	23-428	[»]
	2NPG	X-ray	2.00	A	23-428	[»]
	2NPJ	X-ray	2.00	A	23-428	[»]
	2NPK	X-ray	2.00	A	23-428	[»]
	2NS1	X-ray	1.96	A	23-428	[»]
	2NUU	X-ray	2.50	A/B/C/D/E/F	25-428	[»]
	3C1G	X-ray	2.30	A	23-428	[»]
	3C1H	X-ray	2.20	A	23-428	[»]
	3C1I	X-ray	2.30	A	23-428	[»]
	3C1J	X-ray	2.00	A	23-428	[»]
	6B21	X-ray	2.45	A	26-428	[»]
ProteinModelPortalⁱ	P69681
SMRⁱ	P69681
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4259856, 9 interactors
DIPⁱ	DIP-29874N
IntActⁱ	P69681, 3 interactors
STRINGⁱ	316385.ECDH10B_0407

Chemistry databases

DrugBankⁱ	DB04147 Lauryl Dimethylamine-N-Oxide DB01828 Methylamine

DrugBankⁱ

DB04147 Lauryl Dimethylamine-N-Oxide
DB01828 Methylamine

Protein family/group databases

TCDBⁱ	1.A.11.1.1 the ammonium transporter channel (amt) family

TCDBⁱ

1.A.11.1.1 the ammonium transporter channel (amt) family

Proteomic databases

PaxDbⁱ	P69681
PRIDEⁱ	P69681

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73554; AAC73554; b0451 BAE76231; BAE76231; BAE76231
GeneIDⁱ	945084
KEGGⁱ	ecj:JW0441 eco:b0451
PATRICⁱ	fig\|1411691.4.peg.1824

Organism-specific databases

EchoBASEⁱ	EB1768
EcoGeneⁱ	EG11821 amtB

Phylogenomic databases

eggNOGⁱ	ENOG4105BZU Bacteria ENOG410XNMH LUCA
HOGENOMⁱ	HOG000017736
InParanoidⁱ	P69681
KOⁱ	K03320
PhylomeDBⁱ	P69681

Enzyme and pathway databases

BioCycⁱ	EcoCyc:AMTB-MONOMER MetaCyc:AMTB-MONOMER

BioCycⁱ

EcoCyc:AMTB-MONOMER
MetaCyc:AMTB-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P69681
Protein Ontology More...PROⁱ	PR:P69681

Family and domain databases

Gene3Dⁱ	1.10.3430.10, 1 hit
InterProⁱ	View protein in InterPro IPR029020 Ammonium/urea_transptr IPR001905 Ammonium_transpt IPR018047 Ammonium_transpt_CS IPR024041 NH4_transpt_AmtB-like_dom
Pfamⁱ	View protein in Pfam PF00909 Ammonium_transp, 1 hit
TIGRFAMsⁱ	TIGR00836 amt, 1 hit
PROSITEⁱ	View protein in PROSITE PS01219 AMMONIUM_TRANSP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	AMTB_ECOLI
Accessionⁱ	P69681Primary (citable) accession number: P69681 Secondary accession number(s): P37905, Q2MBX5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
	Last sequence update:	March 29, 2005
	Last modified:	November 7, 2018
	This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P69681 (AMTB_ECOLI)

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Ammonia channel

amtB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ