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Protein

Ammonia channel

Gene

amtB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the uptake of ammonia.

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: UniProtKB
  • identical protein binding Source: IntAct
  • uniporter activity Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processAmmonia transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:AMTB-MONOMER
MetaCyc:AMTB-MONOMER

Protein family/group databases

TCDBi1.A.11.1.1 the ammonium transporter channel (amt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonia channel
Alternative name(s):
Ammonia transporter
Gene namesi
Name:amtB
Synonyms:ybaG
Ordered Locus Names:b0451, JW0441
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11821 amtB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 28Periplasmic6
Transmembranei29 – 55Helical; Name=1Add BLAST27
Topological domaini56 – 59Cytoplasmic4
Transmembranei60 – 90Helical; Name=2Add BLAST31
Topological domaini91 – 118PeriplasmicAdd BLAST28
Transmembranei119 – 141Helical; Name=3Add BLAST23
Topological domaini142 – 146Cytoplasmic5
Transmembranei147 – 170Helical; Name=4Add BLAST24
Topological domaini171 – 184PeriplasmicAdd BLAST14
Transmembranei185 – 203Helical; Name=5Add BLAST19
Topological domaini204 – 215CytoplasmicAdd BLAST12
Transmembranei216 – 242Helical; Name=6Add BLAST27
Topological domaini243 – 245Periplasmic3
Transmembranei246 – 275Helical; Name=7Add BLAST30
Topological domaini276 – 279Cytoplasmic4
Transmembranei280 – 294Helical; Name=8Add BLAST15
Topological domaini295 – 302Periplasmic8
Transmembranei303 – 328Helical; Name=9Add BLAST26
Topological domaini329 – 332Cytoplasmic4
Transmembranei333 – 354Helical; Name=10Add BLAST22
Topological domaini355 – 369PeriplasmicAdd BLAST15
Transmembranei370 – 402Helical; Name=11Add BLAST33
Topological domaini403 – 428CytoplasmicAdd BLAST26

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

DrugBankiDB04147 Lauryl Dimethylamine-N-Oxide
DB01828 Methylamine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000000130723 – 428Ammonia channelAdd BLAST406

Proteomic databases

PaxDbiP69681
PRIDEiP69681

Interactioni

Subunit structurei

Homotrimer. May interact with GlnK.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4259856, 9 interactors
DIPiDIP-29874N
IntActiP69681, 3 interactors
STRINGi316385.ECDH10B_0407

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP69681
SMRiP69681
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69681

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
ENOG410XNMH LUCA
HOGENOMiHOG000017736
InParanoidiP69681
KOiK03320
PhylomeDBiP69681

Family and domain databases

Gene3Di1.10.3430.10, 1 hit
InterProiView protein in InterPro
IPR029020 Ammonium/urea_transptr
IPR001905 Ammonium_transpt
IPR018047 Ammonium_transpt_CS
IPR024041 NH4_transpt_AmtB-like_dom
PfamiView protein in Pfam
PF00909 Ammonium_transp, 1 hit
TIGRFAMsiTIGR00836 amt, 1 hit
PROSITEiView protein in PROSITE
PS01219 AMMONIUM_TRANSP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69681-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKIATIKTGL ASLAMLPGLV MAAPAVADKA DNAFMMICTA LVLFMTIPGI
60 70 80 90 100
ALFYGGLIRG KNVLSMLTQV TVTFALVCIL WVVYGYSLAF GEGNNFFGNI
110 120 130 140 150
NWLMLKNIEL TAVMGSIYQY IHVAFQGSFA CITVGLIVGA LAERIRFSAV
160 170 180 190 200
LIFVVVWLTL SYIPIAHMVW GGGLLASHGA LDFAGGTVVH INAAIAGLVG
210 220 230 240 250
AYLIGKRVGF GKEAFKPHNL PMVFTGTAIL YIGWFGFNAG SAGTANEIAA
260 270 280 290 300
LAFVNTVVAT AAAILGWIFG EWALRGKPSL LGACSGAIAG LVGVTPACGY
310 320 330 340 350
IGVGGALIIG VVAGLAGLWG VTMLKRLLRV DDPCDVFGVH GVCGIVGCIM
360 370 380 390 400
TGIFAASSLG GVGFAEGVTM GHQLLVQLES IAITIVWSGV VAFIGYKLAD
410 420
LTVGLRVPEE QEREGLDVNS HGENAYNA
Length:428
Mass (Da):44,515
Last modified:March 29, 2005 - v1
Checksum:iB4AC96F0E5AE2B59
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti377 – 382QLESIA → SWKASP in M63308 (PubMed:1645722).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA Translation: AAD14837.1
U82664 Genomic DNA Translation: AAB40207.1
U00096 Genomic DNA Translation: AAC73554.1
AP009048 Genomic DNA Translation: BAE76231.1
M63308 Genomic DNA No translation available.
PIRiA90692
C64775
E85542
RefSeqiNP_414985.1, NC_000913.3
WP_000685029.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73554; AAC73554; b0451
BAE76231; BAE76231; BAE76231
GeneIDi945084
KEGGiecj:JW0441
eco:b0451
PATRICifig|1411691.4.peg.1824

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40429 Genomic DNA Translation: AAD14837.1
U82664 Genomic DNA Translation: AAB40207.1
U00096 Genomic DNA Translation: AAC73554.1
AP009048 Genomic DNA Translation: BAE76231.1
M63308 Genomic DNA No translation available.
PIRiA90692
C64775
E85542
RefSeqiNP_414985.1, NC_000913.3
WP_000685029.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U77X-ray2.00A23-407[»]
1U7CX-ray1.85A23-407[»]
1U7GX-ray1.40A23-407[»]
1XQEX-ray2.10A23-428[»]
1XQFX-ray1.80A23-428[»]
2NMRX-ray2.10A23-428[»]
2NOPX-ray2.00A23-428[»]
2NOWX-ray2.20A23-428[»]
2NPCX-ray2.10A23-428[»]
2NPDX-ray2.10A23-428[»]
2NPEX-ray2.10A23-428[»]
2NPGX-ray2.00A23-428[»]
2NPJX-ray2.00A23-428[»]
2NPKX-ray2.00A23-428[»]
2NS1X-ray1.96A23-428[»]
2NUUX-ray2.50A/B/C/D/E/F25-428[»]
3C1GX-ray2.30A23-428[»]
3C1HX-ray2.20A23-428[»]
3C1IX-ray2.30A23-428[»]
3C1JX-ray2.00A23-428[»]
6B21X-ray2.45A26-428[»]
ProteinModelPortaliP69681
SMRiP69681
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259856, 9 interactors
DIPiDIP-29874N
IntActiP69681, 3 interactors
STRINGi316385.ECDH10B_0407

Chemistry databases

DrugBankiDB04147 Lauryl Dimethylamine-N-Oxide
DB01828 Methylamine

Protein family/group databases

TCDBi1.A.11.1.1 the ammonium transporter channel (amt) family

Proteomic databases

PaxDbiP69681
PRIDEiP69681

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73554; AAC73554; b0451
BAE76231; BAE76231; BAE76231
GeneIDi945084
KEGGiecj:JW0441
eco:b0451
PATRICifig|1411691.4.peg.1824

Organism-specific databases

EchoBASEiEB1768
EcoGeneiEG11821 amtB

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
ENOG410XNMH LUCA
HOGENOMiHOG000017736
InParanoidiP69681
KOiK03320
PhylomeDBiP69681

Enzyme and pathway databases

BioCyciEcoCyc:AMTB-MONOMER
MetaCyc:AMTB-MONOMER

Miscellaneous databases

EvolutionaryTraceiP69681
PROiPR:P69681

Family and domain databases

Gene3Di1.10.3430.10, 1 hit
InterProiView protein in InterPro
IPR029020 Ammonium/urea_transptr
IPR001905 Ammonium_transpt
IPR018047 Ammonium_transpt_CS
IPR024041 NH4_transpt_AmtB-like_dom
PfamiView protein in Pfam
PF00909 Ammonium_transp, 1 hit
TIGRFAMsiTIGR00836 amt, 1 hit
PROSITEiView protein in PROSITE
PS01219 AMMONIUM_TRANSP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMTB_ECOLI
AccessioniPrimary (citable) accession number: P69681
Secondary accession number(s): P37905, Q2MBX5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 7, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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