UniProtKB - P69681 (AMTB_ECOLI)
Protein
Ammonia channel
Gene
amtB
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the uptake of ammonia.
GO - Molecular functioni
- ammonium transmembrane transporter activity Source: UniProtKB
- identical protein binding Source: IntAct
- uniporter activity Source: EcoCyc
GO - Biological processi
- ammonium transport Source: UniProtKB
- carbon dioxide transport Source: UniProtKB
- nitrogen utilization Source: GO_Central
Keywordsi
Biological process | Ammonia transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:AMTB-MONOMER MetaCyc:AMTB-MONOMER |
Protein family/group databases
TCDBi | 1.A.11.1.1, the ammonium transporter channel (amt) family |
Names & Taxonomyi
Protein namesi | Recommended name: Ammonia channelAlternative name(s): Ammonia transporter |
Gene namesi | Name:amtB Synonyms:ybaG Ordered Locus Names:b0451, JW0441 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 23 – 28 | Periplasmic | 6 | |
Transmembranei | 29 – 55 | Helical; Name=1Add BLAST | 27 | |
Topological domaini | 56 – 59 | Cytoplasmic | 4 | |
Transmembranei | 60 – 90 | Helical; Name=2Add BLAST | 31 | |
Topological domaini | 91 – 118 | PeriplasmicAdd BLAST | 28 | |
Transmembranei | 119 – 141 | Helical; Name=3Add BLAST | 23 | |
Topological domaini | 142 – 146 | Cytoplasmic | 5 | |
Transmembranei | 147 – 170 | Helical; Name=4Add BLAST | 24 | |
Topological domaini | 171 – 184 | PeriplasmicAdd BLAST | 14 | |
Transmembranei | 185 – 203 | Helical; Name=5Add BLAST | 19 | |
Topological domaini | 204 – 215 | CytoplasmicAdd BLAST | 12 | |
Transmembranei | 216 – 242 | Helical; Name=6Add BLAST | 27 | |
Topological domaini | 243 – 245 | Periplasmic | 3 | |
Transmembranei | 246 – 275 | Helical; Name=7Add BLAST | 30 | |
Topological domaini | 276 – 279 | Cytoplasmic | 4 | |
Transmembranei | 280 – 294 | Helical; Name=8Add BLAST | 15 | |
Topological domaini | 295 – 302 | Periplasmic | 8 | |
Transmembranei | 303 – 328 | Helical; Name=9Add BLAST | 26 | |
Topological domaini | 329 – 332 | Cytoplasmic | 4 | |
Transmembranei | 333 – 354 | Helical; Name=10Add BLAST | 22 | |
Topological domaini | 355 – 369 | PeriplasmicAdd BLAST | 15 | |
Transmembranei | 370 – 402 | Helical; Name=11Add BLAST | 33 | |
Topological domaini | 403 – 428 | CytoplasmicAdd BLAST | 26 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Chemistry databases
DrugBanki | DB04147, Dodecyldimethylamine N-oxide DB01828, Methylamine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | 1 PublicationAdd BLAST | 22 | |
ChainiPRO_0000001307 | 23 – 428 | Ammonia channelAdd BLAST | 406 |
Proteomic databases
jPOSTi | P69681 |
PaxDbi | P69681 |
PRIDEi | P69681 |
Interactioni
Subunit structurei
Homotrimer. May interact with GlnK.
1 PublicationBinary interactionsi
Hide detailsP69681
With | #Exp. | IntAct |
---|---|---|
itself | 4 | EBI-9137905,EBI-9137905 |
glnK [P0AC55] | 3 | EBI-9137905,EBI-559503 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259856, 9 interactors 849473, 3 interactors |
DIPi | DIP-29874N |
IntActi | P69681, 3 interactors |
STRINGi | 511145.b0451 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
PCDDBi | P69681 |
SMRi | P69681 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69681 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0004, Bacteria |
HOGENOMi | CLU_000445_33_0_6 |
InParanoidi | P69681 |
PhylomeDBi | P69681 |
Family and domain databases
Gene3Di | 1.10.3430.10, 1 hit |
InterProi | View protein in InterPro IPR029020, Ammonium/urea_transptr IPR001905, Ammonium_transpt IPR018047, Ammonium_transpt_CS IPR024041, NH4_transpt_AmtB-like_dom |
Pfami | View protein in Pfam PF00909, Ammonium_transp, 1 hit |
TIGRFAMsi | TIGR00836, amt, 1 hit |
PROSITEi | View protein in PROSITE PS01219, AMMONIUM_TRANSP, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P69681-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIATIKTGL ASLAMLPGLV MAAPAVADKA DNAFMMICTA LVLFMTIPGI
60 70 80 90 100
ALFYGGLIRG KNVLSMLTQV TVTFALVCIL WVVYGYSLAF GEGNNFFGNI
110 120 130 140 150
NWLMLKNIEL TAVMGSIYQY IHVAFQGSFA CITVGLIVGA LAERIRFSAV
160 170 180 190 200
LIFVVVWLTL SYIPIAHMVW GGGLLASHGA LDFAGGTVVH INAAIAGLVG
210 220 230 240 250
AYLIGKRVGF GKEAFKPHNL PMVFTGTAIL YIGWFGFNAG SAGTANEIAA
260 270 280 290 300
LAFVNTVVAT AAAILGWIFG EWALRGKPSL LGACSGAIAG LVGVTPACGY
310 320 330 340 350
IGVGGALIIG VVAGLAGLWG VTMLKRLLRV DDPCDVFGVH GVCGIVGCIM
360 370 380 390 400
TGIFAASSLG GVGFAEGVTM GHQLLVQLES IAITIVWSGV VAFIGYKLAD
410 420
LTVGLRVPEE QEREGLDVNS HGENAYNA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 377 – 382 | QLESIA → SWKASP in M63308 (PubMed:1645722).Curated | 6 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40429 Genomic DNA Translation: AAD14837.1 U82664 Genomic DNA Translation: AAB40207.1 U00096 Genomic DNA Translation: AAC73554.1 AP009048 Genomic DNA Translation: BAE76231.1 M63308 Genomic DNA No translation available. |
PIRi | A90692 C64775 E85542 |
RefSeqi | NP_414985.1, NC_000913.3 WP_000685029.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73554; AAC73554; b0451 BAE76231; BAE76231; BAE76231 |
GeneIDi | 52075792 945084 |
KEGGi | ecj:JW0441 eco:b0451 |
PATRICi | fig|1411691.4.peg.1824 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U40429 Genomic DNA Translation: AAD14837.1 U82664 Genomic DNA Translation: AAB40207.1 U00096 Genomic DNA Translation: AAC73554.1 AP009048 Genomic DNA Translation: BAE76231.1 M63308 Genomic DNA No translation available. |
PIRi | A90692 C64775 E85542 |
RefSeqi | NP_414985.1, NC_000913.3 WP_000685029.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1U77 | X-ray | 2.00 | A | 23-407 | [»] | |
1U7C | X-ray | 1.85 | A | 23-407 | [»] | |
1U7G | X-ray | 1.40 | A | 23-407 | [»] | |
1XQE | X-ray | 2.10 | A | 23-428 | [»] | |
1XQF | X-ray | 1.80 | A | 23-428 | [»] | |
2NMR | X-ray | 2.10 | A | 23-428 | [»] | |
2NOP | X-ray | 2.00 | A | 23-428 | [»] | |
2NOW | X-ray | 2.20 | A | 23-428 | [»] | |
2NPC | X-ray | 2.10 | A | 23-428 | [»] | |
2NPD | X-ray | 2.10 | A | 23-428 | [»] | |
2NPE | X-ray | 2.10 | A | 23-428 | [»] | |
2NPG | X-ray | 2.00 | A | 23-428 | [»] | |
2NPJ | X-ray | 2.00 | A | 23-428 | [»] | |
2NPK | X-ray | 2.00 | A | 23-428 | [»] | |
2NS1 | X-ray | 1.96 | A | 23-428 | [»] | |
2NUU | X-ray | 2.50 | A/B/C/D/E/F | 25-428 | [»] | |
3C1G | X-ray | 2.30 | A | 23-428 | [»] | |
3C1H | X-ray | 2.20 | A | 23-428 | [»] | |
3C1I | X-ray | 2.30 | A | 23-428 | [»] | |
3C1J | X-ray | 2.00 | A | 23-428 | [»] | |
6B21 | X-ray | 2.45 | A | 26-428 | [»] | |
PCDDBi | P69681 | |||||
SMRi | P69681 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259856, 9 interactors 849473, 3 interactors |
DIPi | DIP-29874N |
IntActi | P69681, 3 interactors |
STRINGi | 511145.b0451 |
Chemistry databases
DrugBanki | DB04147, Dodecyldimethylamine N-oxide DB01828, Methylamine |
Protein family/group databases
TCDBi | 1.A.11.1.1, the ammonium transporter channel (amt) family |
Proteomic databases
jPOSTi | P69681 |
PaxDbi | P69681 |
PRIDEi | P69681 |
Genome annotation databases
EnsemblBacteriai | AAC73554; AAC73554; b0451 BAE76231; BAE76231; BAE76231 |
GeneIDi | 52075792 945084 |
KEGGi | ecj:JW0441 eco:b0451 |
PATRICi | fig|1411691.4.peg.1824 |
Organism-specific databases
EchoBASEi | EB1768 |
Phylogenomic databases
eggNOGi | COG0004, Bacteria |
HOGENOMi | CLU_000445_33_0_6 |
InParanoidi | P69681 |
PhylomeDBi | P69681 |
Enzyme and pathway databases
BioCyci | EcoCyc:AMTB-MONOMER MetaCyc:AMTB-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P69681 |
PROi | PR:P69681 |
Family and domain databases
Gene3Di | 1.10.3430.10, 1 hit |
InterProi | View protein in InterPro IPR029020, Ammonium/urea_transptr IPR001905, Ammonium_transpt IPR018047, Ammonium_transpt_CS IPR024041, NH4_transpt_AmtB-like_dom |
Pfami | View protein in Pfam PF00909, Ammonium_transp, 1 hit |
TIGRFAMsi | TIGR00836, amt, 1 hit |
PROSITEi | View protein in PROSITE PS01219, AMMONIUM_TRANSP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMTB_ECOLI | |
Accessioni | P69681Primary (citable) accession number: P69681 Secondary accession number(s): P37905, Q2MBX5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | March 29, 2005 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families