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Protein

Protein ORF3

Gene

ORF3

Organism
Hepatitis E virus genotype 1 (isolate Human/Burma) (HEV-1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. Acts also as a viroporin and forms ion conductive pores allowing viral particle release. Impairs the generation of type I interferon by downregulating host TLR3 and TLR7 as well as their downstream signaling pathways.By similarity

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Inhibition of host innate immune response by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ORF3
Short name:
pORF3
Gene namesi
ORF Names:ORF3
OrganismiHepatitis E virus genotype 1 (isolate Human/Burma) (HEV-1)
Taxonomic identifieri31767 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageHepeviridaeOrthohepevirusOrthohepevirus A
Virus hostiBandicota bengalensis (lesser bandicoot rat) [TaxID: 69079]
Callithrix [TaxID: 9481]
Cercopithecus hamlyni (Owl-faced monkey) (Hamlyn's monkey) [TaxID: 9536]
Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Gallus gallus (Chicken) [TaxID: 9031]
Homo sapiens (Human) [TaxID: 9606]
Macaca (macaques) [TaxID: 9539]
Mus musculus (Mouse) [TaxID: 10090]
Pan troglodytes (Chimpanzee) [TaxID: 9598]
Saimiri (squirrel monkeys) [TaxID: 9520]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000007243 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host endoplasmic reticulum, Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001361 – 114Protein ORF3Add BLAST114

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71Phosphoserine; by hostBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Form homooligomers (By similarity). Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2 (via SH3 domain); binding does not activate the kinases. Interacts with host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts with host HPX/hemopexin. Interacts (when phosphorylated) with capsid protein ORF2. Interacts with host TSG101; this interaction plays a role in viral release from the host cell (By similarity).By similarity

Protein-protein interaction databases

MINTiP69616

Structurei

3D structure databases

SMRiP69616
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 22HydrophobicAdd BLAST17
Regioni28 – 68Interaction with host HPXBy similarityAdd BLAST41
Regioni33 – 53HydrophobicAdd BLAST21
Regioni48 – 72Interaction with the capsid proteinBy similarityAdd BLAST25
Regioni72 – 114Homodimerization, and interaction with host AMBP/bikuninBy similarityAdd BLAST43
Regioni95 – 104Interaction with host SRC, HCK, FYN, PIK3R3 and GRB2By similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 22Cys-richAdd BLAST17
Compositional biasi55 – 112Pro-richAdd BLAST58

Sequence similaritiesi

Belongs to the hepevirus ORF3 protein family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090001YP

Family and domain databases

InterProiView protein in InterPro
IPR003384 HEV_Orf2
PfamiView protein in Pfam
PF02444 HEV_ORF1, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006561 HEV_ORF2, 1 hit

Sequencei

Sequence statusi: Complete.

P69616-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGSRPCALGL FCCCSSCFCL CCPRHRPVSR LAAVVGGAAA VPAVVSGVTG
60 70 80 90 100
LILSPSQSPI FIQPTPSPPM SPLRPGLDLV FANPPDHSAP LGVTRPSAPP
110
LPHVVDLPQL GPRR
Length:114
Mass (Da):11,712
Last modified:May 20, 2008 - v2
Checksum:iC5AC40F482A1D7F6
GO

Sequence cautioni

The sequence AAA45735 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73218 Genomic RNA Translation: AAA45735.1 Different initiation.
PIRiB40778 VHWWHE

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73218 Genomic RNA Translation: AAA45735.1 Different initiation.
PIRiB40778 VHWWHE

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZZQX-ray3.81A61-114[»]
SMRiP69616
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiP69616

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090001YP

Family and domain databases

InterProiView protein in InterPro
IPR003384 HEV_Orf2
PfamiView protein in Pfam
PF02444 HEV_ORF1, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD006561 HEV_ORF2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiORF3_HEVBU
AccessioniPrimary (citable) accession number: P69616
Secondary accession number(s): P29325
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: May 20, 2008
Last modified: November 7, 2018
This is version 46 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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