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Protein

Transmembrane protease serine 9

Gene

Tmprss9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Serase-1 and serase-2 are serine proteases that hydrolyze the peptides N-t-Boc-Gln-Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC. In contrast, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Ala-Pro-Ala-AMC are not significantly hydrolyzed (By similarity).By similarity

Enzyme regulationi

Inhibited by serine protease inhibitors PMSF and 4-(2-aminoethyl)benzenesulfonyl fluoride, but not by EDTA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei245Charge relay systemBy similarity1
Active sitei294Charge relay systemBy similarity1
Active sitei389Charge relay systemBy similarity1
Active sitei546Charge relay systemBy similarity1
Active sitei594Charge relay systemBy similarity1
Active sitei689Charge relay systemBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.969

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protease serine 9 (EC:3.4.21.-)
Alternative name(s):
Polyserase-I
Polyserine protease 1
Short name:
Polyserase-1
Cleaved into the following 3 chains:
Gene namesi
Name:Tmprss9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1309581 Tmprss9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini3 – 31CytoplasmicSequence analysisAdd BLAST29
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Topological domaini53 – 1061ExtracellularSequence analysisAdd BLAST1009

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000278751 – 1061Transmembrane protease serine 9Sequence analysisAdd BLAST1061
ChainiPRO_0000027876205 – 505Serase-1Sequence analysisAdd BLAST301
ChainiPRO_0000027877506 – 829Serase-2Sequence analysisAdd BLAST324
ChainiPRO_0000027878804 – 1061Serase-3Sequence analysisAdd BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi156 ↔ 168By similarity
Disulfide bondi163 ↔ 182By similarity
Disulfide bondi176 ↔ 191By similarity
Disulfide bondi230 ↔ 246By similarity
Disulfide bondi328 ↔ 395By similarity
Disulfide bondi360 ↔ 374By similarity
Disulfide bondi385 ↔ 414By similarity
Glycosylationi469N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi531 ↔ 547By similarity
Glycosylationi549N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi628 ↔ 695By similarity
Glycosylationi640N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi660 ↔ 674By similarity
Glycosylationi665N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi685 ↔ 714By similarity
Glycosylationi791N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi856 ↔ 872By similarity
Disulfide bondi951 ↔ 1017By similarity
Disulfide bondi982 ↔ 996By similarity
Disulfide bondi1007 ↔ 1036By similarity

Post-translational modificationi

Proteolytically cleaved to generate 3 independent serine protease chains. The cleaved chains may remain attached to the membrane thanks to disulfide bonds. It is unclear whether cleavage always takes place (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei204 – 205CleavageSequence analysis2
Sitei505 – 506CleavageSequence analysis2
Sitei829 – 830CleavageSequence analysis2

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP69526
PRIDEiP69526

Expressioni

Gene expression databases

BgeeiENSRNOG00000032429
ExpressionAtlasiP69526 baseline and differential

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000041782

Structurei

3D structure databases

ProteinModelPortaliP69526
SMRiP69526
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini155 – 192LDL-receptor class APROSITE-ProRule annotationAdd BLAST38
Domaini205 – 438Peptidase S1 1PROSITE-ProRule annotationAdd BLAST234
Domaini506 – 738Peptidase S1 2PROSITE-ProRule annotationAdd BLAST233
Domaini830 – 1060Peptidase S1 3PROSITE-ProRule annotationAdd BLAST231

Domaini

The serine protease 1 and 2 domains are catalytically active, whereas the serine protease 3 domain lacks the essential Ser residue of the catalytic triad at position 1011 and is predicted to be inactive.By similarity

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118962
HOGENOMiHOG000154654
HOVERGENiHBG079270
InParanoidiP69526
KOiK09640
OMAiPDCGLAP
OrthoDBiEOG091G0DF7
PhylomeDBiP69526

Family and domain databases

CDDicd00112 LDLa, 1 hit
cd00190 Tryp_SPc, 3 hits
InterProiView protein in InterPro
IPR036055 LDL_receptor-like_sf
IPR002172 LDrepeatLR_classA_rpt
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR017324 Tmprss9
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00057 Ldl_recept_a, 1 hit
PF00089 Trypsin, 3 hits
PIRSFiPIRSF037931 TMPRSS9_polyserase-1, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00192 LDLa, 1 hit
SM00020 Tryp_SPc, 3 hits
SUPFAMiSSF50494 SSF50494, 3 hits
SSF57424 SSF57424, 1 hit
PROSITEiView protein in PROSITE
PS01209 LDLRA_1, 1 hit
PS50068 LDLRA_2, 1 hit
PS50240 TRYPSIN_DOM, 3 hits
PS00134 TRYPSIN_HIS, 3 hits
PS00135 TRYPSIN_SER, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPAAPDLQP VPEVTKGVPV PTPDSGCCRA AVTTVVAISV ASLTLGVLSA
60 70 80 90 100
FLSAQGVQVE HTAQLHGVRF TSLLQQENSD FYRLLTPALQ TLLHFLLRAL
110 120 130 140 150
QPLSLDQEAD ILQKGIQARL QGQGLSLAAY GTITSVELTG RCEGPVTERD
160 170 180 190 200
LKSGHCPGNA FSCQNSQCVS KENPECDDRV DCSDGSDEAQ CDCGWQPAWR
210 220 230 240 250
SAGRIVGGAE AAPGEFPWQV SLRENHEHFC GATIIGARWL VSAAHCFNEF
260 270 280 290 300
QDPAQWAAQA GSVHLSGSEA SAVRARVLRI AKHPAYNADT ADFDVAVLEL
310 320 330 340 350
ARPLPFGRYV QPACLPAATH VFPPRKKCLI SGWGYLKEDF LVKPEVLQKA
360 370 380 390 400
TVELLDQNLC SSLYGHSLTD RMVCAGYLDG KVDSCQGDSG GPLVCEEPSG
410 420 430 440 450
RFFLAGVVSW GIGCAEARRP GVYTRVTRLR DWILEVTSSA DTPVVPTEAP
460 470 480 490 500
APITPSTPWP TSPESRVPNT TAKPTVAPTP APLHPSTAAK PQECGARPAM
510 520 530 540 550
DKPTRIVGGI SAVSGEVPWQ ASLKEGSRHF CGATVVGDRW LLSAAHCFNH
560 570 580 590 600
TKLEQVQAHL GTVSLLGVGG SPVKLGLRSV ALHPRYNPGI LDFDVALLEL
610 620 630 640 650
AQPLVFNKYI QPVCLPLAIH KFPVGRKCMI SGWGNMQEGN ATKPDILQKA
660 670 680 690 700
SVGIIEQKMC GALYNFSLTD RMLCAGFLEG RVDSCQGDSG GPLACEETPG
710 720 730 740 750
VFYLAGIVSW GIGCAQAKKP GVYARITRLK DWILKAMSSD PSSTAHPHTS
760 770 780 790 800
STRLIPSQPP TTTAAGLIPE ASTGRPATLR ATIRVTTRPL NTTLSARSTT
810 820 830 840 850
TRRQTPAPGT TVFSHLPDCG LAPPGALTRI VGGSAASLGE WPWQVSLWLR
860 870 880 890 900
RREHRCGAVL VAERWLLSAA HCFDVYGDPM QWAAFLGTPF LSSTEGQLER
910 920 930 940 950
VARIYRHPFY NIYTLDYDVA LLELAGPVRR SRLVRPICLP GPTRPPEGAR
960 970 980 990 1000
CVITGWGSLR EGGSMARQLQ KAAVRVLSEQ TCRRFYPVQI SSRMLCAGFP
1010 1020 1030 1040 1050
QGGVDSCSGD AGGPLACREP SGQWVLTGVT SWGYGCGRPH FPGVYTRVAA
1060
VLGWIGQNIR E
Length:1,061
Mass (Da):113,890
Last modified:March 15, 2005 - v1
Checksum:i3319B65E62FF4CAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03056045 Genomic DNA No translation available.
RefSeqiNP_001040565.2, NM_001047100.2
UniGeneiRn.123548

Genome annotation databases

EnsembliENSRNOT00000049221; ENSRNOP00000041782; ENSRNOG00000032429
GeneIDi314636
KEGGirno:314636
UCSCiRGD:1309581 rat

Similar proteinsi

Entry informationi

Entry nameiTMPS9_RAT
AccessioniPrimary (citable) accession number: P69526
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 20, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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