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UniProtKB - P69525 (TMPS9_MOUSE)
Protein
Transmembrane protease serine 9
Gene
Tmprss9
Organism
Mus musculus (Mouse)
Status
Functioni
Serase-1 and serase-2 are serine proteases that hydrolyze the peptides N-t-Boc-Gln-Ala-Arg-AMC and N-t-Boc-Gln-Gly-Arg-AMC. In contrast, N-t-Boc-Ala-Phe-Lys-AMC and N-t-Boc-Ala-Pro-Ala-AMC are not significantly hydrolyzed (By similarity).
By similarityActivity regulationi
Inhibited by serine protease inhibitors PMSF and 4-(2-aminoethyl)benzenesulfonyl fluoride, but not by EDTA.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 245 | Charge relay systemBy similarity | 1 | |
Active sitei | 294 | Charge relay systemBy similarity | 1 | |
Active sitei | 389 | Charge relay systemBy similarity | 1 | |
Active sitei | 546 | Charge relay systemBy similarity | 1 | |
Active sitei | 594 | Charge relay systemBy similarity | 1 | |
Active sitei | 689 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
- serine-type peptidase activity Source: MGI
GO - Biological processi
- plasminogen activation Source: MGI
- proteolysis Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Tmprss9 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:3612246, Tmprss9 |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type II membrane protein By similarity
Plasma Membrane
- integral component of plasma membrane Source: InterPro
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 3 – 31 | CytoplasmicSequence analysisAdd BLAST | 29 | |
Transmembranei | 32 – 52 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 53 – 1065 | ExtracellularSequence analysisAdd BLAST | 1013 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000027871 | 1 – 1065 | Transmembrane protease serine 9Sequence analysisAdd BLAST | 1065 | |
ChainiPRO_0000027872 | 205 – 505 | Serase-1Sequence analysisAdd BLAST | 301 | |
ChainiPRO_0000027873 | 506 – 833 | Serase-2Sequence analysisAdd BLAST | 328 | |
ChainiPRO_0000027874 | 834 – 1065 | Serase-3Sequence analysisAdd BLAST | 232 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 156 ↔ 168 | By similarity | ||
Disulfide bondi | 163 ↔ 182 | By similarity | ||
Disulfide bondi | 176 ↔ 191 | By similarity | ||
Disulfide bondi | 230 ↔ 246 | By similarity | ||
Disulfide bondi | 328 ↔ 395 | By similarity | ||
Disulfide bondi | 360 ↔ 374 | By similarity | ||
Disulfide bondi | 385 ↔ 414 | By similarity | ||
Disulfide bondi | 531 ↔ 547 | By similarity | ||
Glycosylationi | 549 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 628 ↔ 695 | By similarity | ||
Glycosylationi | 640 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 660 ↔ 674 | By similarity | ||
Glycosylationi | 665 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 685 ↔ 714 | By similarity | ||
Glycosylationi | 792 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 860 ↔ 876 | By similarity | ||
Disulfide bondi | 955 ↔ 1021 | By similarity | ||
Disulfide bondi | 986 ↔ 1000 | By similarity | ||
Disulfide bondi | 1011 ↔ 1040 | By similarity |
Post-translational modificationi
Proteolytically cleaved to generate 3 independent serine protease chains. The cleaved chains may remain attached to the membrane thanks to disulfide bonds. It is unclear whether cleavage always takes place (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 204 – 205 | CleavageSequence analysis | 2 | |
Sitei | 505 – 506 | CleavageSequence analysis | 2 | |
Sitei | 833 – 834 | CleavageSequence analysis | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P69525 |
PRIDEi | P69525 |
ProteomicsDBi | 259268 |
PTM databases
GlyGeni | P69525, 4 sites |
iPTMneti | P69525 |
PhosphoSitePlusi | P69525 |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000100970 |
Miscellaneous databases
RNActi | P69525, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 155 – 192 | LDL-receptor class APROSITE-ProRule annotationAdd BLAST | 38 | |
Domaini | 205 – 438 | Peptidase S1 1PROSITE-ProRule annotationAdd BLAST | 234 | |
Domaini | 506 – 738 | Peptidase S1 2PROSITE-ProRule annotationAdd BLAST | 233 | |
Domaini | 834 – 1064 | Peptidase S1 3PROSITE-ProRule annotationAdd BLAST | 231 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 445 – 500 | DisorderedSequence analysisAdd BLAST | 56 | |
Regioni | 740 – 762 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 788 – 814 | DisorderedSequence analysisAdd BLAST | 27 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 448 – 462 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 742 – 758 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 788 – 813 | Polar residuesSequence analysisAdd BLAST | 26 |
Domaini
The serine protease 1 and 2 domains are catalytically active, whereas the serine protease 3 domain lacks the essential Ser residue of the catalytic triad at position 1015 and is predicted to be inactive.By similarity
Sequence similaritiesi
Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Keywords - Domaini
Repeat, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
InParanoidi | P69525 |
PhylomeDBi | P69525 |
Family and domain databases
CDDi | cd00112, LDLa, 1 hit cd00190, Tryp_SPc, 3 hits |
Gene3Di | 2.40.10.10, 4 hits 4.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR036055, LDL_receptor-like_sf IPR002172, LDrepeatLR_classA_rpt IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR017324, Tmprss9 IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00057, Ldl_recept_a, 1 hit PF00089, Trypsin, 3 hits |
PIRSFi | PIRSF037931, TMPRSS9_polyserase-1, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00192, LDLa, 1 hit SM00020, Tryp_SPc, 3 hits |
SUPFAMi | SSF50494, SSF50494, 3 hits SSF57424, SSF57424, 1 hit |
PROSITEi | View protein in PROSITE PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS50240, TRYPSIN_DOM, 3 hits PS00134, TRYPSIN_HIS, 3 hits PS00135, TRYPSIN_SER, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P69525-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPTAPDLQL VPEVTKAVSV SAPDPGPCRT AVIAVVGISM AIVTLGVLSA
60 70 80 90 100
FFSAQGAHVE HIAELHGIRF TSSLQQENSD FYRLLTHALQ TLLHFLLRAL
110 120 130 140 150
QPLSLKQEAD ILQKGIQARL QEQGLSLAAY GTIVSVELTG RCEGPVTERD
160 170 180 190 200
LKSGHCPGNV FSCQNGQCVS KENPECDDRV DCSDESDEAQ CDCGWQPAWR
210 220 230 240 250
SAGRIVGGVE AAPGEFPWQV SLRENHEHFC GATIIGARWL VSAAHCFNEF
260 270 280 290 300
QDPAQWAAQA GSVHLSGSEA SAVRTRVLRI AKHPAYDADT ADFDVAVLEL
310 320 330 340 350
ARPLPFGRYV QPACLPAATH VFPPGKKCLI SGWGYLKEDF LVKPEVLQKA
360 370 380 390 400
TVELLDQSLC SSLYGHSLTD RMVCAGYLDG KVDSCQGDSG GPLVCEEPSG
410 420 430 440 450
RFFLAGIVSW GIGCAEARRP GVYTRVTRLR DWILEVTSAA DMPVVPTATP
460 470 480 490 500
APATPSTPWP TSPESWAPNT FAKPTAAPSP VPLHPSTTAK PQECGARPAM
510 520 530 540 550
DKPTRIVGGI SAVSGEVPWQ ASLKEGPRHF CGATVVGDRW LLSAAHCFNH
560 570 580 590 600
TKVEQVQAHL GTVSLLGVGG SPVKLGLRRV ALHPRYNPGI LDFDVALLEL
610 620 630 640 650
AQPLVFNKYI QPVCLPLAIH KFPVGRKCMI SGWGNMQEGN ATKPDILQKA
660 670 680 690 700
SVGIIEQKMC GALYNFSLTD RMLCAGFLEG RVDSCQGDSG GPLACEETPG
710 720 730 740 750
VFYLAGIVSW GIGCAQAKKP GVYARITRLK DWILKAMSSD PSSMARPHTS
760 770 780 790 800
STRLIPSEPP KTTAAGLIIP EATTSRLATP RATIRVTTRP LNTTLSARST
810 820 830 840 850
TTRGQTAAPS APGTTIHSHL PDCGLAPPGA LTRIVGGSAA SLGEWPWQVS
860 870 880 890 900
LWLRRREHRC GAVLVAERWL LSAAHCFDIY GDPMQWAAFL GTPFLSSTEG
910 920 930 940 950
QLERVARIYR HPFYNIYTLD YDVALLELAG PVRRSRLVRP ICLPGPARPP
960 970 980 990 1000
DGARCVITGW GSLREGGSMA RQLQKAAVRV LSEQTCRRFY PVQISSRMLC
1010 1020 1030 1040 1050
AGFPQGGVDS CSGDAGGPLA CREPSGQWVL TGVTSWGYGC GRPHFPGVYT
1060
RVAAVLGWIG QNIQE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3YTR8 | D3YTR8_MOUSE | Transmembrane protease serine 9 | Tmprss9 | 1,099 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC152413 Genomic DNA No translation available. AC166937 Genomic DNA No translation available. |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC152413 Genomic DNA No translation available. AC166937 Genomic DNA No translation available. |
3D structure databases
SMRi | P69525 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000100970 |
PTM databases
GlyGeni | P69525, 4 sites |
iPTMneti | P69525 |
PhosphoSitePlusi | P69525 |
Proteomic databases
PaxDbi | P69525 |
PRIDEi | P69525 |
ProteomicsDBi | 259268 |
Organism-specific databases
MGIi | MGI:3612246, Tmprss9 |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
InParanoidi | P69525 |
PhylomeDBi | P69525 |
Miscellaneous databases
ChiTaRSi | Tmprss9, mouse |
PROi | PR:P69525 |
RNActi | P69525, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd00112, LDLa, 1 hit cd00190, Tryp_SPc, 3 hits |
Gene3Di | 2.40.10.10, 4 hits 4.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR036055, LDL_receptor-like_sf IPR002172, LDrepeatLR_classA_rpt IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR017324, Tmprss9 IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00057, Ldl_recept_a, 1 hit PF00089, Trypsin, 3 hits |
PIRSFi | PIRSF037931, TMPRSS9_polyserase-1, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00192, LDLa, 1 hit SM00020, Tryp_SPc, 3 hits |
SUPFAMi | SSF50494, SSF50494, 3 hits SSF57424, SSF57424, 1 hit |
PROSITEi | View protein in PROSITE PS01209, LDLRA_1, 1 hit PS50068, LDLRA_2, 1 hit PS50240, TRYPSIN_DOM, 3 hits PS00134, TRYPSIN_HIS, 3 hits PS00135, TRYPSIN_SER, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | TMPS9_MOUSE | |
Accessioni | P69525Primary (citable) accession number: P69525 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | March 15, 2005 | |
Last modified: | September 29, 2021 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families