UniProtKB - P69490 (CCME_ECOLI)
Protein
Cytochrome c-type biogenesis protein CcmE
Gene
ccmE
Organism
Escherichia coli (strain K12)
Status
Functioni
Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 130 | Heme; covalent, via pros nitrogen | 1 | |
Metal bindingi | 134 | Iron (heme axial ligand) | 1 |
GO - Molecular functioni
- heme binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- cytochrome c-heme linkage Source: EcoCyc
- heme transport Source: EcoCyc
Keywordsi
Biological process | Cytochrome c-type biogenesis |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:CCME-MONOMER MetaCyc:CCME-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome c-type biogenesis protein CcmEUniRule annotationAlternative name(s): Cytochrome c maturation protein EUniRule annotation Heme chaperone CcmEUniRule annotation |
Gene namesi | Name:ccmEUniRule annotation Synonyms:yejS Ordered Locus Names:b2197, JW2185 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation; Single-pass type II membrane protein UniRule annotation; Periplasmic side UniRule annotation
Note: Stabilized by CcmD in the membrane.
Plasma Membrane
- intrinsic component of periplasmic side of plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicUniRule annotation | 8 | |
Transmembranei | 9 – 29 | Helical; Signal-anchor for type II membrane proteinUniRule annotationAdd BLAST | 21 | |
Topological domaini | 30 – 159 | PeriplasmicUniRule annotationAdd BLAST | 130 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 130 | H → A: Abolishes heme binding. 1 Publication | 1 | |
Mutagenesisi | 130 | H → C: Can still form a covalent bond with heme, but blocks heme release transfer to cytochrome c. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201575 | 1 – 159 | Cytochrome c-type biogenesis protein CcmEAdd BLAST | 159 |
Proteomic databases
jPOSTi | P69490 |
PaxDbi | P69490 |
PRIDEi | P69490 |
Interactioni
Subunit structurei
Forms a ternary complex with CcmC and CcmD.
Interacts with CcmF. Shuttles between CcmC and CcmF for heme delivery.
3 PublicationsBinary interactionsi
P69490
With | #Exp. | IntAct |
---|---|---|
ccmC [P0ABM1] | 7 | EBI-1128007,EBI-2123469 |
Protein-protein interaction databases
BioGRIDi | 4260483, 134 interactors |
ComplexPortali | CPX-3568, CcmABCDE system I cytochrome c biogenesis complex |
DIPi | DIP-9255N |
IntActi | P69490, 8 interactors |
MINTi | P69490 |
STRINGi | 511145.b2197 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P69490 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69490 |
Family & Domainsi
Sequence similaritiesi
Belongs to the CcmE/CycJ family.UniRule annotation
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2332, Bacteria |
HOGENOMi | CLU_079503_1_0_6 |
InParanoidi | P69490 |
PhylomeDBi | P69490 |
Family and domain databases
DisProti | DP01729 |
HAMAPi | MF_01959, CcmE, 1 hit |
InterProi | View protein in InterPro IPR004329, CcmE IPR036127, CcmE-like_sf |
PANTHERi | PTHR34128, PTHR34128, 1 hit |
Pfami | View protein in Pfam PF03100, CcmE, 1 hit |
SUPFAMi | SSF82093, SSF82093, 1 hit |
i Sequence
Sequence statusi: Complete.
P69490-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNIRRKNRLW IACAVLAGLA LTIGLVLYAL RSNIDLFYTP GEILYGKRET
60 70 80 90 100
QQMPEVGQRL RVGGMVMPGS VQRDPNSLKV TFTIYDAEGS VDVSYEGILP
110 120 130 140 150
DLFREGQGVV VQGELEKGNH ILAKEVLAKH DENYTPPEVE KAMEANHRRP
ASVYKDPAS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16389.1 U00096 Genomic DNA Translation: AAC75257.1 AP009048 Genomic DNA Translation: BAE76660.1 |
PIRi | C64989 |
RefSeqi | NP_416701.1, NC_000913.3 WP_001026418.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75257; AAC75257; b2197 BAE76660; BAE76660; BAE76660 |
GeneIDi | 58462687 946697 |
KEGGi | ecj:JW2185 eco:b2197 |
PATRICi | fig|1411691.4.peg.39 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00008 Genomic DNA Translation: AAA16389.1 U00096 Genomic DNA Translation: AAC75257.1 AP009048 Genomic DNA Translation: BAE76660.1 |
PIRi | C64989 |
RefSeqi | NP_416701.1, NC_000913.3 WP_001026418.1, NZ_STEB01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SR3 | NMR | - | A | 30-159 | [»] | |
SMRi | P69490 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260483, 134 interactors |
ComplexPortali | CPX-3568, CcmABCDE system I cytochrome c biogenesis complex |
DIPi | DIP-9255N |
IntActi | P69490, 8 interactors |
MINTi | P69490 |
STRINGi | 511145.b2197 |
Proteomic databases
jPOSTi | P69490 |
PaxDbi | P69490 |
PRIDEi | P69490 |
Genome annotation databases
EnsemblBacteriai | AAC75257; AAC75257; b2197 BAE76660; BAE76660; BAE76660 |
GeneIDi | 58462687 946697 |
KEGGi | ecj:JW2185 eco:b2197 |
PATRICi | fig|1411691.4.peg.39 |
Organism-specific databases
EchoBASEi | EB1986 |
Phylogenomic databases
eggNOGi | COG2332, Bacteria |
HOGENOMi | CLU_079503_1_0_6 |
InParanoidi | P69490 |
PhylomeDBi | P69490 |
Enzyme and pathway databases
BioCyci | EcoCyc:CCME-MONOMER MetaCyc:CCME-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P69490 |
PROi | PR:P69490 |
Family and domain databases
DisProti | DP01729 |
HAMAPi | MF_01959, CcmE, 1 hit |
InterProi | View protein in InterPro IPR004329, CcmE IPR036127, CcmE-like_sf |
PANTHERi | PTHR34128, PTHR34128, 1 hit |
Pfami | View protein in Pfam PF03100, CcmE, 1 hit |
SUPFAMi | SSF82093, SSF82093, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCME_ECOLI | |
Accessioni | P69490Primary (citable) accession number: P69490 Secondary accession number(s): P33928, Q2MAP6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | March 15, 2005 | |
Last modified: | April 7, 2021 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families