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UniProtKB - P69452 (LCFA_ECOL6)
Protein
Long-chain-fatty-acid--CoA ligase
Gene
fadD
Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Functioni
Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids.
By similarityMiscellaneous
Activity is the highest with fatty acid substrates of > 10 carbon atoms.By similarity
Catalytic activityi
- EC:6.2.1.3By similarity
Cofactori
Mg2+By similarity
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 213 – 224 | ATPCuratedAdd BLAST | 12 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- long-chain fatty acid-CoA ligase activity Source: UniProtKB-EC
GO - Biological processi
- fatty acid beta-oxidation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Ligase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | Recommended name: Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3By similarity)Alternative name(s): Long-chain acyl-CoA synthetase Short name: Acyl-CoA synthetase |
Gene namesi | Name:fadD Ordered Locus Names:c2209 |
Organismi | Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) |
Taxonomic identifieri | 199310 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Membrane By similarity; Peripheral membrane protein By similarity
Note: Partially membrane-associated.By similarity
Other locations
- membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193126 | 1 – 561 | Long-chain-fatty-acid--CoA ligaseAdd BLAST | 561 |
Family & Domainsi
Sequence similaritiesi
Belongs to the ATP-dependent AMP-binding enzyme family.Curated
Phylogenomic databases
eggNOGi | COG0318, Bacteria |
HOGENOMi | CLU_000022_59_9_6 |
OMAi | KQSDMKA |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
P69452-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKVWLNRYP ADVPTEINPD RYQSLVDMFE QSVARYADQP AFVNMGEVMT
60 70 80 90 100
FRKLEERSRA FAAYLQQGLG LKKGDRVALM MPNLLQYPVA LFGILRAGMI
110 120 130 140 150
VVNVNPLYTP RELEHQLNDS GASAIVIVSN FAHTLEKVVD KTAVQHVILT
160 170 180 190 200
RMGDQLSTAK GTVVNFVVKY IKRLVPKYHL PDAISFRSAL HNGYRMQYVK
210 220 230 240 250
PELVPEDLAF LQYTGGTTGV AKGAMLTHRN MLANLEQVNA TYGPLLHPGK
260 270 280 290 300
ELVVTALPLY HIFALTINCL LFIELGGQNL LITNPRDIPG LVKELAKYPF
310 320 330 340 350
TAITGVNTLF NALLNNKEFQ QLDFSSLHLS AGGGMPVQQV VAERWVKLTG
360 370 380 390 400
QYLLEGYGLT ECAPLVSVNP YDIDYHSGSI GLPVPSTEAK LVDDDDNEVP
410 420 430 440 450
PGQPGELCVK GPQVMLGYWQ RPDATDEIIK NGWLHTGDIA VMDEEGFLRI
460 470 480 490 500
VDRKKDMILV SGFNVYPNEI EDVVMQHPGV QEVAAVGVPS GSSGEAVKIF
510 520 530 540 550
VVKKDPSLTE ESLVTFCRRQ LTGYKVPKLV EFRDELPKSN VGKILRRELR
560
DEARGKVDNK A
Sequence cautioni
The sequence AAN80668 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014075 Genomic DNA Translation: AAN80668.1 Different initiation. |
PIRi | E64941, S41589 |
RefSeqi | WP_000758422.1, NC_004431.1 |
Genome annotation databases
EnsemblBacteriai | AAN80668; AAN80668; c2209 |
GeneIDi | 66674306 |
KEGGi | ecc:c2209 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014075 Genomic DNA Translation: AAN80668.1 Different initiation. |
PIRi | E64941, S41589 |
RefSeqi | WP_000758422.1, NC_004431.1 |
3D structure databases
SMRi | P69452 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 199310.c2209 |
Genome annotation databases
EnsemblBacteriai | AAN80668; AAN80668; c2209 |
GeneIDi | 66674306 |
KEGGi | ecc:c2209 |
Phylogenomic databases
eggNOGi | COG0318, Bacteria |
HOGENOMi | CLU_000022_59_9_6 |
OMAi | KQSDMKA |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
Family and domain databases
Gene3Di | 3.30.300.30, 1 hit 3.40.50.12780, 1 hit |
InterProi | View protein in InterPro IPR025110, AMP-bd_C IPR045851, AMP-bd_C_sf IPR020845, AMP-binding_CS IPR000873, AMP-dep_Synth/Lig IPR042099, ANL_N_sf |
Pfami | View protein in Pfam PF00501, AMP-binding, 1 hit PF13193, AMP-binding_C, 1 hit |
PROSITEi | View protein in PROSITE PS00455, AMP_BINDING, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LCFA_ECOL6 | |
Accessioni | P69452Primary (citable) accession number: P69452 Secondary accession number(s): P29212 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | March 1, 2005 | |
Last modified: | February 23, 2022 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families