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UniProtKB - P69432 (PGAD_ECOLI)
Protein
Biofilm PGA synthesis protein PgaD
Gene
pgaD
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for the synthesis of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA.
3 PublicationsGO - Biological processi
- cell adhesion involved in biofilm formation Source: ComplexPortal
- cell adhesion involved in single-species biofilm formation Source: InterPro
Enzyme and pathway databases
BioCyci | EcoCyc:G6528-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Biofilm PGA synthesis protein PgaD |
Gene namesi | Name:pgaD Synonyms:ycdP Ordered Locus Names:b1021, JW1006 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 23 | CytoplasmicSequence analysisAdd BLAST | 23 | |
Transmembranei | 24 – 44 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 45 – 53 | PeriplasmicSequence analysis | 9 | |
Transmembranei | 54 – 74 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 75 – 137 | CytoplasmicSequence analysisAdd BLAST | 63 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Cells lacking this gene do not synthesize PGA.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058349 | 1 – 137 | Biofilm PGA synthesis protein PgaDAdd BLAST | 137 |
Proteomic databases
PaxDbi | P69432 |
PRIDEi | P69432 |
Expressioni
Inductioni
Levels of this protein are positively controlled by the second messenger c-di-GMP (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to increased levels of PgaD.1 Publication
Interactioni
Binary interactionsi
Protein-protein interaction databases
BioGRIDi | 4260055, 21 interactors 851820, 7 interactors |
ComplexPortali | CPX-3743, pgaCD beta-glycosyltransferase complex |
DIPi | DIP-48114N |
IntActi | P69432, 7 interactors |
MINTi | P69432 |
STRINGi | 511145.b1021 |
Structurei
3D structure databases
AlphaFoldDBi | P69432 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Family & Domainsi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3658, Bacteria ENOG50338G7, Bacteria |
HOGENOMi | CLU_131509_0_0_6 |
OMAi | VFWESEA |
Family and domain databases
InterProi | View protein in InterPro IPR023829, PGA_PgaD |
Pfami | View protein in Pfam PF13994, PgaD, 1 hit |
TIGRFAMsi | TIGR03940, PGA_PgaD, 1 hit |
i Sequence
Sequence statusi: Complete.
P69432-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNNLIITTRQ SPVRLLVDYV ATTILWTLFA LFIFLFAMDL LTGYYWQSEA
60 70 80 90 100
RSRLQFYFLL AVANAVVLIV WALYNKLRFQ KQQHHAAYQY TPQEYAESLA
110 120 130
IPDELYQQLQ KSHRMSVHFT SQGQIKMVVS EKALVRA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74106.1 AP009048 Genomic DNA Translation: BAA35802.1 |
PIRi | C64844 |
RefSeqi | NP_415540.1, NC_000913.3 WP_001061095.1, NZ_STEB01000006.1 |
Genome annotation databases
EnsemblBacteriai | AAC74106; AAC74106; b1021 BAA35802; BAA35802; BAA35802 |
GeneIDi | 58391625 947503 |
KEGGi | ecj:JW1006 eco:b1021 |
PATRICi | fig|1411691.4.peg.1248 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74106.1 AP009048 Genomic DNA Translation: BAA35802.1 |
PIRi | C64844 |
RefSeqi | NP_415540.1, NC_000913.3 WP_001061095.1, NZ_STEB01000006.1 |
3D structure databases
AlphaFoldDBi | P69432 |
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260055, 21 interactors 851820, 7 interactors |
ComplexPortali | CPX-3743, pgaCD beta-glycosyltransferase complex |
DIPi | DIP-48114N |
IntActi | P69432, 7 interactors |
MINTi | P69432 |
STRINGi | 511145.b1021 |
Proteomic databases
PaxDbi | P69432 |
PRIDEi | P69432 |
Protocols and materials databases
DNASUi | 947503 |
Genome annotation databases
EnsemblBacteriai | AAC74106; AAC74106; b1021 BAA35802; BAA35802; BAA35802 |
GeneIDi | 58391625 947503 |
KEGGi | ecj:JW1006 eco:b1021 |
PATRICi | fig|1411691.4.peg.1248 |
Organism-specific databases
EchoBASEi | EB3622 |
Phylogenomic databases
eggNOGi | COG3658, Bacteria ENOG50338G7, Bacteria |
HOGENOMi | CLU_131509_0_0_6 |
OMAi | VFWESEA |
Enzyme and pathway databases
BioCyci | EcoCyc:G6528-MONOMER |
Miscellaneous databases
PROi | PR:P69432 |
Family and domain databases
InterProi | View protein in InterPro IPR023829, PGA_PgaD |
Pfami | View protein in Pfam PF13994, PgaD, 1 hit |
TIGRFAMsi | TIGR03940, PGA_PgaD, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PGAD_ECOLI | |
Accessioni | P69432Primary (citable) accession number: P69432 Secondary accession number(s): P75904 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | March 1, 2005 | |
Last modified: | May 25, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |