fieF

Functionⁱ

Iron-efflux transporter responsible for iron detoxification. Also able to transport Zn²⁺ in a proton-dependent manner.1 Publication

GO - Molecular functionⁱ

cadmium ion transmembrane transporter activity
Source: EcoCyc
Inferred from direct assayⁱ
- 16049012
ferrous iron transmembrane transporter activity
Source: EcoCyc
Inferred from direct assayⁱ
- 15549269
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 19749753
zinc efflux active transmembrane transporter activity
Source: EcoCyc
Inferred from direct assayⁱ
- 15549269

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cadmium ion transport
Source: EcoCyc
Inferred from direct assayⁱ
- 16049012
cellular cadmium ion homeostasis
Source: EcoCyc
Inferred from direct assayⁱ
- 16049012
cellular zinc ion homeostasis
Source: EcoCyc
Inferred from direct assayⁱ
- 15549269
ferrous iron transport
Source: EcoCyc
Inferred from direct assayⁱ
- 15549269
iron ion homeostasis Source: UniProtKB-KW
zinc ion transport
Source: EcoCyc
Inferred from direct assayⁱ
- 15549269

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Ion transport, Iron transport, Transport
Ligand	Iron

Enzyme and pathway databases

BioCycⁱ	EcoCyc:YIIP-MONOMER MetaCyc:YIIP-MONOMER

BioCycⁱ

EcoCyc:YIIP-MONOMER
MetaCyc:YIIP-MONOMER

Protein family/group databases

TCDBⁱ	2.A.4.7.1 the cation diffusion facilitator (cdf) family

TCDBⁱ

2.A.4.7.1 the cation diffusion facilitator (cdf) family

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ferrous-iron efflux pump FieF
Gene namesⁱ	Name:fieF Synonyms:yiiP Ordered Locus Names:b3915, JW3886
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11873 fieF

EcoGeneⁱ

EG11873 fieF

Subcellular locationⁱ

Cell inner membrane ; Multi-pass membrane protein

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 11	CytoplasmicSequence analysisAdd BLAST	11
Transmembraneⁱ	12 – 34	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	35 – 37	PeriplasmicSequence analysis	3
Transmembraneⁱ	38 – 60	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	61 – 79	CytoplasmicSequence analysisAdd BLAST	19
Transmembraneⁱ	80 – 102	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	103 – 116	PeriplasmicSequence analysisAdd BLAST	14
Transmembraneⁱ	117 – 139	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	140 – 157	CytoplasmicSequence analysisAdd BLAST	18
Transmembraneⁱ	158 – 175	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	176 – 179	PeriplasmicSequence analysis	4
Transmembraneⁱ	180 – 199	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	200 – 300	CytoplasmicSequence analysisAdd BLAST	101

GO - Cellular componentⁱ

integral component of membrane Source: UniProtKB-KW
plasma membrane
Source: EcoCyc
Inferred from direct assayⁱ
- 15919996

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000206124	1 – 300	Ferrous-iron efflux pump FieFAdd BLAST		300

Proteomic databases

PaxDbⁱ	P69380
PRIDEⁱ	P69380

Interactionⁱ

Subunit structureⁱ

Homodimer.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-15802920,EBI-15802920

With

Entry

#Exp.

IntAct

Notes

itself

2

EBI-15802920,EBI-15802920

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- 19749753

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4261076, 7 interactors
Database of interacting proteins More...DIPⁱ	DIP-48564N
STRINGⁱ	316385.ECDH10B_4104

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	12 – 21	Combined sources	10
Helixⁱ	24 – 29	Combined sources	6
Beta strandⁱ	33 – 37	Combined sources	5
Turnⁱ	42 – 44	Combined sources	3
Helixⁱ	45 – 63	Combined sources	19
Helixⁱ	78 – 103	Combined sources	26
Beta strandⁱ	105 – 108	Combined sources	4
Helixⁱ	119 – 141	Combined sources	23
Helixⁱ	145 – 158	Combined sources	14
Beta strandⁱ	166 – 168	Combined sources	3
Helixⁱ	180 – 205	Combined sources	26
Helixⁱ	212 – 224	Combined sources	13
Beta strandⁱ	225 – 227	Combined sources	3
Beta strandⁱ	229 – 239	Combined sources	11
Beta strandⁱ	242 – 250	Combined sources	9
Helixⁱ	257 – 274	Combined sources	18
Beta strandⁱ	279 – 285	Combined sources	7

3D structure databases

ProteinModelPortalⁱ	P69380
SMRⁱ	P69380
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P69380

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. FieF subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG41076HS Bacteria COG0053 LUCA
HOGENOMⁱ	HOG000069673
InParanoidⁱ	P69380
KEGG Orthology (KO) More...KOⁱ	K13283
OMAⁱ	NCHDIAS
PhylomeDBⁱ	P69380

Family and domain databases

Gene3Dⁱ	1.20.1510.10, 1 hit 3.30.70.1350, 1 hit
HAMAPⁱ	MF_01425 Cation_efflux_FieF, 1 hit
InterProⁱ	View protein in InterPro IPR002524 Cation_efflux IPR027470 Cation_efflux_CTD IPR036837 Cation_efflux_CTD_sf IPR023783 Cation_efflux_FieF IPR027469 Cation_efflux_TMD_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01545 Cation_efflux, 1 hit PF16916 ZT_dimer, 1 hit
SUPFAMⁱ	SSF160240 SSF160240, 1 hit SSF161111 SSF161111, 1 hit
TIGRFAMsⁱ	TIGR01297 CDF, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P69380-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNQSYGRLVS RAAIAATAMA SLLLLIKIFA WWYTGSVSIL AALVDSLVDI 
        60         70         80         90        100
GASLTNLLVV RYSLQPADDN HSFGHGKAES LAALAQSMFI SGSALFLFLT 
       110        120        130        140        150
GIQHLISPTP MTDPGVGVIV TIVALICTII LVSFQRWVVR RTQSQAVRAD 
       160        170        180        190        200
MLHYQSDVMM NGAILLALGL SWYGWHRADA LFALGIGIYI LYSALRMGYE 
       210        220        230        240        250
AVQSLLDRAL PDEERQEIID IVTSWPGVSG AHDLRTRQSG PTRFIQIHLE 
       260        270        280        290        300
MEDSLPLVQA HMVADQVEQA ILRRFPGSDV IIHQDPCSVV PREGKRSMLS

Length:300

Mass (Da):32,927

Last modified:March 1, 2005 - v1

Checksum:ⁱA9ECC4D6ED9A750F

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L19201 Genomic DNA Translation: AAB03047.1 U00096 Genomic DNA Translation: AAC76897.1 AP009048 Genomic DNA Translation: BAE77395.1
PIRⁱ	S40858
NCBI Reference Sequences More...RefSeqⁱ	NP_418350.1, NC_000913.3 WP_001076742.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC76897; AAC76897; b3915 BAE77395; BAE77395; BAE77395
GeneIDⁱ	948413
KEGGⁱ	ecj:JW3886 eco:b3915
PATRICⁱ	fig\|1411691.4.peg.2790

Protein	Similar proteins		Species	Score	Length
P69380	Ferrous-iron efflux pump FieF		ECO24		300
Ferrous-iron efflux pump FieF		ECO55		300
Ferrous-iron efflux pump FieF		ECO57		300
Ferrous-iron efflux pump FieF		ECO5E		300
Ferrous-iron efflux pump FieF		ECO8A		300
+114

Protein	Similar proteins		Species	Score	Length
P69380	Cation-efflux pump FieF		SALTI		298
CDF family cation-efflux pump FieF		Lelliottia sp. WB101		301
Cation-efflux pump FieF		Cedecea lapagei		301
Ferrous-iron efflux pump FieF		ECO45		300
Ferrous-iron efflux pump FieF		ECO7I		300
+593

Protein	Similar proteins		Species	Score	Length
P69380	Cation-efflux pump FieF		SALTI		298
CDF family cation-efflux pump FieF		Lelliottia sp. WB101		301
Cation-efflux pump FieF		Cedecea lapagei		301
UPI0009AFF0E3		SALCE		300
Cation-efflux pump FieF		Citrobacter sp. MGH106		300
+1559

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	L19201 Genomic DNA Translation: AAB03047.1 U00096 Genomic DNA Translation: AAC76897.1 AP009048 Genomic DNA Translation: BAE77395.1
PIRⁱ	S40858
NCBI Reference Sequences More...RefSeqⁱ	NP_418350.1, NC_000913.3 WP_001076742.1, NZ_LN832404.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2QFI	X-ray	3.80	A/B	1-300	[»]
	3H90	X-ray	2.90	A/B/C/D	8-290	[»]
ProteinModelPortalⁱ	P69380
SMRⁱ	P69380
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261076, 7 interactors
Database of interacting proteins More...DIPⁱ	DIP-48564N
STRINGⁱ	316385.ECDH10B_4104

Protein family/group databases

TCDBⁱ	2.A.4.7.1 the cation diffusion facilitator (cdf) family

TCDBⁱ

2.A.4.7.1 the cation diffusion facilitator (cdf) family

Proteomic databases

PaxDbⁱ	P69380
PRIDEⁱ	P69380

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76897; AAC76897; b3915 BAE77395; BAE77395; BAE77395
GeneIDⁱ	948413
KEGGⁱ	ecj:JW3886 eco:b3915
PATRICⁱ	fig\|1411691.4.peg.2790

Organism-specific databases

EchoBASEⁱ	EB1819
EcoGeneⁱ	EG11873 fieF

Phylogenomic databases

eggNOGⁱ	ENOG41076HS Bacteria COG0053 LUCA
HOGENOMⁱ	HOG000069673
InParanoidⁱ	P69380
KEGG Orthology (KO) More...KOⁱ	K13283
OMAⁱ	NCHDIAS
PhylomeDBⁱ	P69380

Enzyme and pathway databases

BioCycⁱ	EcoCyc:YIIP-MONOMER MetaCyc:YIIP-MONOMER

BioCycⁱ

EcoCyc:YIIP-MONOMER
MetaCyc:YIIP-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P69380
Protein Ontology More...PROⁱ	PR:P69380

Family and domain databases

Gene3Dⁱ	1.20.1510.10, 1 hit 3.30.70.1350, 1 hit
HAMAPⁱ	MF_01425 Cation_efflux_FieF, 1 hit
InterProⁱ	View protein in InterPro IPR002524 Cation_efflux IPR027470 Cation_efflux_CTD IPR036837 Cation_efflux_CTD_sf IPR023783 Cation_efflux_FieF IPR027469 Cation_efflux_TMD_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01545 Cation_efflux, 1 hit PF16916 ZT_dimer, 1 hit
SUPFAMⁱ	SSF160240 SSF160240, 1 hit SSF161111 SSF161111, 1 hit
TIGRFAMsⁱ	TIGR01297 CDF, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	FIEF_ECOLI
Accessionⁱ	P69380Primary (citable) accession number: P69380 Secondary accession number(s): P32159, Q2M8L1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
	Last sequence update:	March 1, 2005
	Last modified:	May 23, 2018
	This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P69380 (FIEF_ECOLI)

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Ferrous-iron efflux pump FieF

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ