Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ferrous-iron efflux pump FieF

Gene

fieF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Iron-efflux transporter responsible for iron detoxification. Also able to transport Zn2+ in a proton-dependent manner.1 Publication

GO - Molecular functioni

  • cadmium ion transmembrane transporter activity Source: EcoCyc
  • ferrous iron transmembrane transporter activity Source: EcoCyc
  • identical protein binding Source: IntAct
  • zinc efflux active transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cadmium ion transport Source: EcoCyc
  • cellular cadmium ion homeostasis Source: EcoCyc
  • cellular zinc ion homeostasis Source: EcoCyc
  • ferrous iron transport Source: EcoCyc
  • iron ion homeostasis Source: UniProtKB-KW
  • zinc ion transport Source: EcoCyc

Keywordsi

Biological processIon transport, Iron transport, Transport
LigandIron

Enzyme and pathway databases

BioCyciEcoCyc:YIIP-MONOMER
MetaCyc:YIIP-MONOMER

Protein family/group databases

TCDBi2.A.4.7.1 the cation diffusion facilitator (cdf) family

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrous-iron efflux pump FieF
Gene namesi
Name:fieF
Synonyms:yiiP
Ordered Locus Names:b3915, JW3886
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11873 fieF

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 34HelicalSequence analysisAdd BLAST23
Topological domaini35 – 37PeriplasmicSequence analysis3
Transmembranei38 – 60HelicalSequence analysisAdd BLAST23
Topological domaini61 – 79CytoplasmicSequence analysisAdd BLAST19
Transmembranei80 – 102HelicalSequence analysisAdd BLAST23
Topological domaini103 – 116PeriplasmicSequence analysisAdd BLAST14
Transmembranei117 – 139HelicalSequence analysisAdd BLAST23
Topological domaini140 – 157CytoplasmicSequence analysisAdd BLAST18
Transmembranei158 – 175HelicalSequence analysisAdd BLAST18
Topological domaini176 – 179PeriplasmicSequence analysis4
Transmembranei180 – 199HelicalSequence analysisAdd BLAST20
Topological domaini200 – 300CytoplasmicSequence analysisAdd BLAST101

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002061241 – 300Ferrous-iron efflux pump FieFAdd BLAST300

Proteomic databases

PaxDbiP69380
PRIDEiP69380

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-15802920,EBI-15802920

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4261076, 7 interactors
DIPiDIP-48564N
STRINGi316385.ECDH10B_4104

Structurei

Secondary structure

1300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 21Combined sources10
Helixi24 – 29Combined sources6
Beta strandi33 – 37Combined sources5
Turni42 – 44Combined sources3
Helixi45 – 63Combined sources19
Helixi78 – 103Combined sources26
Beta strandi105 – 108Combined sources4
Helixi119 – 141Combined sources23
Helixi145 – 158Combined sources14
Beta strandi166 – 168Combined sources3
Helixi180 – 205Combined sources26
Helixi212 – 224Combined sources13
Beta strandi225 – 227Combined sources3
Beta strandi229 – 239Combined sources11
Beta strandi242 – 250Combined sources9
Helixi257 – 274Combined sources18
Beta strandi279 – 285Combined sources7

3D structure databases

ProteinModelPortaliP69380
SMRiP69380
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69380

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41076HS Bacteria
COG0053 LUCA
HOGENOMiHOG000069673
InParanoidiP69380
KOiK13283
OMAiNCHDIAS
PhylomeDBiP69380

Family and domain databases

Gene3Di1.20.1510.10, 1 hit
3.30.70.1350, 1 hit
HAMAPiMF_01425 Cation_efflux_FieF, 1 hit
InterProiView protein in InterPro
IPR002524 Cation_efflux
IPR027470 Cation_efflux_CTD
IPR036837 Cation_efflux_CTD_sf
IPR023783 Cation_efflux_FieF
IPR027469 Cation_efflux_TMD_sf
PfamiView protein in Pfam
PF01545 Cation_efflux, 1 hit
PF16916 ZT_dimer, 1 hit
SUPFAMiSSF160240 SSF160240, 1 hit
SSF161111 SSF161111, 1 hit
TIGRFAMsiTIGR01297 CDF, 1 hit

Sequencei

Sequence statusi: Complete.

P69380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQSYGRLVS RAAIAATAMA SLLLLIKIFA WWYTGSVSIL AALVDSLVDI
60 70 80 90 100
GASLTNLLVV RYSLQPADDN HSFGHGKAES LAALAQSMFI SGSALFLFLT
110 120 130 140 150
GIQHLISPTP MTDPGVGVIV TIVALICTII LVSFQRWVVR RTQSQAVRAD
160 170 180 190 200
MLHYQSDVMM NGAILLALGL SWYGWHRADA LFALGIGIYI LYSALRMGYE
210 220 230 240 250
AVQSLLDRAL PDEERQEIID IVTSWPGVSG AHDLRTRQSG PTRFIQIHLE
260 270 280 290 300
MEDSLPLVQA HMVADQVEQA ILRRFPGSDV IIHQDPCSVV PREGKRSMLS
Length:300
Mass (Da):32,927
Last modified:March 1, 2005 - v1
Checksum:iA9ECC4D6ED9A750F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA Translation: AAB03047.1
U00096 Genomic DNA Translation: AAC76897.1
AP009048 Genomic DNA Translation: BAE77395.1
PIRiS40858
RefSeqiNP_418350.1, NC_000913.3
WP_001076742.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76897; AAC76897; b3915
BAE77395; BAE77395; BAE77395
GeneIDi948413
KEGGiecj:JW3886
eco:b3915
PATRICifig|1411691.4.peg.2790

Similar proteinsi

Entry informationi

Entry nameiFIEF_ECOLI
AccessioniPrimary (citable) accession number: P69380
Secondary accession number(s): P32159, Q2M8L1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 23, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health