UniProtKB - P69380 (FIEF_ECOLI)
Protein
Ferrous-iron efflux pump FieF
Gene
fieF
Organism
Escherichia coli (strain K12)
Status
Functioni
Iron-efflux transporter responsible for iron detoxification. Also able to transport Zn2+ in a proton-dependent manner.1 Publication
GO - Molecular functioni
- cadmium ion transmembrane transporter activity Source: EcoCyc
- cation transmembrane transporter activity Source: GO_Central
- ferrous iron transmembrane transporter activity Source: EcoCyc
- identical protein binding Source: IntAct
- zinc efflux active transmembrane transporter activity Source: EcoCyc
GO - Biological processi
- cadmium ion transport Source: EcoCyc
- cellular cadmium ion homeostasis Source: EcoCyc
- cellular iron ion homeostasis Source: GO_Central
- cellular zinc ion homeostasis Source: EcoCyc
- iron ion transport Source: EcoCyc
- zinc ion transport Source: EcoCyc
Keywordsi
Biological process | Ion transport, Iron transport, Transport |
Ligand | Iron |
Enzyme and pathway databases
BioCyci | EcoCyc:YIIP-MONOMER MetaCyc:YIIP-MONOMER |
Protein family/group databases
TCDBi | 2.A.4.7.1, the cation diffusion facilitator (cdf) family |
Names & Taxonomyi
Protein namesi | Recommended name: Ferrous-iron efflux pump FieF |
Gene namesi | Name:fieF Synonyms:yiiP Ordered Locus Names:b3915, JW3886 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 12 – 34 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 35 – 37 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 38 – 60 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 61 – 79 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 80 – 102 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 103 – 116 | PeriplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 117 – 139 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 140 – 157 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 158 – 175 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 176 – 179 | PeriplasmicSequence analysis | 4 | |
Transmembranei | 180 – 199 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 200 – 300 | CytoplasmicSequence analysisAdd BLAST | 101 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206124 | 1 – 300 | Ferrous-iron efflux pump FieFAdd BLAST | 300 |
Proteomic databases
PaxDbi | P69380 |
PRIDEi | P69380 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationBinary interactionsi
P69380
With | #Exp. | IntAct |
---|---|---|
itself | 2 | EBI-15802920,EBI-15802920 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261076, 7 interactors |
DIPi | DIP-48564N |
STRINGi | 511145.b3915 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P69380 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69380 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. FieF subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0053, Bacteria |
HOGENOMi | CLU_013430_3_0_6 |
InParanoidi | P69380 |
PhylomeDBi | P69380 |
Family and domain databases
Gene3Di | 1.20.1510.10, 1 hit 3.30.70.1350, 1 hit |
HAMAPi | MF_01425, Cation_efflux_FieF, 1 hit |
InterProi | View protein in InterPro IPR002524, Cation_efflux IPR027470, Cation_efflux_CTD IPR036837, Cation_efflux_CTD_sf IPR023783, Cation_efflux_FieF IPR027469, Cation_efflux_TMD_sf |
Pfami | View protein in Pfam PF01545, Cation_efflux, 1 hit PF16916, ZT_dimer, 1 hit |
SUPFAMi | SSF160240, SSF160240, 1 hit SSF161111, SSF161111, 1 hit |
TIGRFAMsi | TIGR01297, CDF, 1 hit |
i Sequence
Sequence statusi: Complete.
P69380-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNQSYGRLVS RAAIAATAMA SLLLLIKIFA WWYTGSVSIL AALVDSLVDI
60 70 80 90 100
GASLTNLLVV RYSLQPADDN HSFGHGKAES LAALAQSMFI SGSALFLFLT
110 120 130 140 150
GIQHLISPTP MTDPGVGVIV TIVALICTII LVSFQRWVVR RTQSQAVRAD
160 170 180 190 200
MLHYQSDVMM NGAILLALGL SWYGWHRADA LFALGIGIYI LYSALRMGYE
210 220 230 240 250
AVQSLLDRAL PDEERQEIID IVTSWPGVSG AHDLRTRQSG PTRFIQIHLE
260 270 280 290 300
MEDSLPLVQA HMVADQVEQA ILRRFPGSDV IIHQDPCSVV PREGKRSMLS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19201 Genomic DNA Translation: AAB03047.1 U00096 Genomic DNA Translation: AAC76897.1 AP009048 Genomic DNA Translation: BAE77395.1 |
PIRi | S40858 |
RefSeqi | NP_418350.1, NC_000913.3 WP_001076742.1, NZ_SSZK01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC76897; AAC76897; b3915 BAE77395; BAE77395; BAE77395 |
GeneIDi | 57732244 948413 |
KEGGi | ecj:JW3886 eco:b3915 |
PATRICi | fig|1411691.4.peg.2790 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19201 Genomic DNA Translation: AAB03047.1 U00096 Genomic DNA Translation: AAC76897.1 AP009048 Genomic DNA Translation: BAE77395.1 |
PIRi | S40858 |
RefSeqi | NP_418350.1, NC_000913.3 WP_001076742.1, NZ_SSZK01000014.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2QFI | X-ray | 3.80 | A/B | 1-300 | [»] | |
3H90 | X-ray | 2.90 | A/B/C/D | 8-290 | [»] | |
SMRi | P69380 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261076, 7 interactors |
DIPi | DIP-48564N |
STRINGi | 511145.b3915 |
Protein family/group databases
TCDBi | 2.A.4.7.1, the cation diffusion facilitator (cdf) family |
Proteomic databases
PaxDbi | P69380 |
PRIDEi | P69380 |
Genome annotation databases
EnsemblBacteriai | AAC76897; AAC76897; b3915 BAE77395; BAE77395; BAE77395 |
GeneIDi | 57732244 948413 |
KEGGi | ecj:JW3886 eco:b3915 |
PATRICi | fig|1411691.4.peg.2790 |
Organism-specific databases
EchoBASEi | EB1819 |
Phylogenomic databases
eggNOGi | COG0053, Bacteria |
HOGENOMi | CLU_013430_3_0_6 |
InParanoidi | P69380 |
PhylomeDBi | P69380 |
Enzyme and pathway databases
BioCyci | EcoCyc:YIIP-MONOMER MetaCyc:YIIP-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P69380 |
PROi | PR:P69380 |
Family and domain databases
Gene3Di | 1.20.1510.10, 1 hit 3.30.70.1350, 1 hit |
HAMAPi | MF_01425, Cation_efflux_FieF, 1 hit |
InterProi | View protein in InterPro IPR002524, Cation_efflux IPR027470, Cation_efflux_CTD IPR036837, Cation_efflux_CTD_sf IPR023783, Cation_efflux_FieF IPR027469, Cation_efflux_TMD_sf |
Pfami | View protein in Pfam PF01545, Cation_efflux, 1 hit PF16916, ZT_dimer, 1 hit |
SUPFAMi | SSF160240, SSF160240, 1 hit SSF161111, SSF161111, 1 hit |
TIGRFAMsi | TIGR01297, CDF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FIEF_ECOLI | |
Accessioni | P69380Primary (citable) accession number: P69380 Secondary accession number(s): P32159, Q2M8L1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | March 1, 2005 | |
Last modified: | April 7, 2021 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families