UniProtKB - P69291 (NCAP_I89A2)
Protein
Nucleoprotein
Gene
NP
Organism
Influenza A virus (strain A/Beijing/353/1989 H3N2)
Status
Functioni
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.UniRule annotation
GO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
- structural molecule activity Source: UniProtKB-UniRule
GO - Biological processi
- viral entry into host cell Source: UniProtKB-KW
- viral penetration into host nucleus Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding, Viral nucleoprotein |
Biological process | Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell |
Names & Taxonomyi
Protein namesi | Recommended name: NucleoproteinUniRule annotationAlternative name(s): Nucleocapsid proteinUniRule annotation Short name: Protein NUniRule annotation |
Gene namesi | Name:NPUniRule annotation |
Organismi | Influenza A virus (strain A/Beijing/353/1989 H3N2) |
Taxonomic identifieri | 380949 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Insthoviricetes › Articulavirales › Orthomyxoviridae › Alphainfluenzavirus › |
Virus hosti | Aves [TaxID: 8782] Cetacea (whales) [TaxID: 9721] Homo sapiens (Human) [TaxID: 9606] Phocidae (true seals) [TaxID: 9709] Sus scrofa (Pig) [TaxID: 9823] |
Proteomesi |
|
Subcellular locationi
- Virion UniRule annotation
- Host nucleus UniRule annotation
GO - Cellular componenti
- helical viral capsid Source: UniProtKB-UniRule
- host cell nucleus Source: UniProtKB-SubCell
- viral nucleocapsid Source: UniProtKB-UniRule
Keywords - Cellular componenti
Capsid protein, Helical capsid protein, Host nucleus, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079025 | 1 – 498 | NucleoproteinAdd BLAST | 498 |
Post-translational modificationi
Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.UniRule annotation
Interactioni
Subunit structurei
Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.
UniRule annotationFamily & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1 – 18 | Unconventional nuclear localization signalUniRule annotationAdd BLAST | 18 | |
Motifi | 198 – 216 | Bipartite nuclear localization signalUniRule annotationAdd BLAST | 19 |
Sequence similaritiesi
Belongs to the influenza viruses nucleoprotein family.UniRule annotation
Family and domain databases
HAMAPi | MF_04070, INFV_NCAP, 1 hit |
InterProi | View protein in InterPro IPR002141, Flu_NP |
Pfami | View protein in Pfam PF00506, Flu_NP, 1 hit |
i Sequence
Sequence statusi: Complete.
P69291-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASQGTKRSY EQMETDGERQ NATEIRASVG KMIDGIGRFY IQMCTELKLS
60 70 80 90 100
DYEGRLIQNS LTVERMVLSA FDERRNRYLE EHPSAGKDPK KTGGPIYKRV
110 120 130 140 150
GGRWMRELVL YDKEEIRRIW RQANNGDDAT RGLTHMMIWH SNLNDTTYQR
160 170 180 190 200
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGIGTMV MELIRMIKRG
210 220 230 240 250
INDRNFWRGE NGRKTRSAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN
260 270 280 290 300
AEIEDLIFSA RSALILRGSV AHKSCLPACV YGPAVSSGYD FEKEGYSLVG
310 320 330 340 350
IDPFKLLQNS QVYSLIRPNE NPAHKSQLVW MACHSAAFED LRLLSFIRGT
360 370 380 390 400
KVSPRGKLST RGVQIASNEN MDNMESSTLE LRSRYWAIRT RSGGNTNQQR
410 420 430 440 450
ASAGQISVQP TFSVQRNLPF EKSTVMAAFT GNTEGRTSDM RAEIIRMMEG
460 470 480 490
AKPEEVSFRG RGVFELSDEK ATNPIVPSFD MSNEGSYFFG DNAEEYDN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07354 Genomic RNA Translation: AAA51484.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L07354 Genomic RNA Translation: AAA51484.1 |
3D structure databases
SMRi | P69291 |
ModBasei | Search... |
Family and domain databases
HAMAPi | MF_04070, INFV_NCAP, 1 hit |
InterProi | View protein in InterPro IPR002141, Flu_NP |
Pfami | View protein in Pfam PF00506, Flu_NP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCAP_I89A2 | |
Accessioni | P69291Primary (citable) accession number: P69291 Secondary accession number(s): Q07539, Q08029 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | March 1, 2005 | |
Last modified: | December 2, 2020 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |