UniProtKB - P69249 (RBS_TOBAC)
Ribulose bisphosphate carboxylase small subunit, chloroplastic
RBCS
Functioni
RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate (PubMed:3681999).
Both reactions occur simultaneously and in competition at the same active site (Probable). Involved in antiviral defenses (By similarity).
By similarityCurated1 PublicationMiscellaneous
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- photorespiration Source: UniProtKB-KW
- reductive pentose-phosphate cycle Source: UniProtKB-KW
Keywordsi
Biological process | Antiviral defense, Calvin cycle, Carbon dioxide fixation, Photorespiration, Photosynthesis |
Enzyme and pathway databases
BRENDAi | 4.1.1.39, 3645 |
SABIO-RKi | P69249 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribulose bisphosphate carboxylase small subunit, chloroplastic1 PublicationUniRule annotationShort name: RuBisCO small subunit1 PublicationUniRule annotation Alternative name(s): TSSU3-81 Publication |
Gene namesi | Name:RBCS1 PublicationUniRule annotation |
Organismi | Nicotiana tabacum (Common tobacco) |
Taxonomic identifieri | 4097 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana |
Proteomesi |
|
Subcellular locationi
Chloroplast
- chloroplast UniRule annotationCurated
Other locations
- plasmodesma By similarity
Note: (Microbial infection) May be present in virus replication complexes (VRCs) of tobamovirus infected cells.By similarity
Chloroplast
- chloroplast Source: UniProtKB-SubCell
Other locations
- plasmodesma Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 57 | Chloroplast1 PublicationAdd BLAST | 57 | |
Transit peptidei | 1 – 56 | ChloroplastUniRule annotationAdd BLAST | 56 | |
ChainiPRO_0000031560 | 57 – 180 | Ribulose bisphosphate carboxylase small subunit, chloroplasticUniRule annotationAdd BLAST | 124 |
Proteomic databases
PRIDEi | P69249 |
Interactioni
Subunit structurei
Heterohexadecamer of 8 large and 8 small subunits.
UniRule annotation2 Publications(Microbial infection) Binds to tobamovirus movement protein; this interaction seems required for viral systemic movement.
By similarityCuratedBinary interactionsi
P69249
With | #Exp. | IntAct |
---|---|---|
TOC159 [O81283] from Arabidopsis thaliana. | 5 | EBI-1766821,EBI-639063 |
TOC34 [Q38906] from Arabidopsis thaliana. | 6 | EBI-1766821,EBI-1766808 |
Protein-protein interaction databases
DIPi | DIP-40635N |
IntActi | P69249, 4 interactors |
MINTi | P69249 |
STRINGi | 4097.P69249 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P69249 |
SMRi | P69249 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P69249 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
OMAi | KQCQVLS |
OrthoDBi | 1258997at2759 |
PhylomeDBi | P69249 |
Family and domain databases
CDDi | cd03527, RuBisCO_small, 1 hit |
Gene3Di | 3.30.190.10, 1 hit |
HAMAPi | MF_00859, RuBisCO_S_bact, 1 hit |
InterProi | View protein in InterPro IPR024681, RuBisCO_ssu IPR000894, RuBisCO_ssu_dom IPR024680, RuBisCO_ssu_N IPR036385, RuBisCO_ssu_sf |
PANTHERi | PTHR31262, PTHR31262, 1 hit |
Pfami | View protein in Pfam PF12338, RbcS, 1 hit PF00101, RuBisCO_small, 1 hit |
PRINTSi | PR00152, RUBISCOSMALL |
SMARTi | View protein in SMART SM00961, RuBisCO_small, 1 hit |
SUPFAMi | SSF55239, SSF55239, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MASSVLSSAA VATRSNVAQA NMVAPFTGLK SAASFPVSRK QNLDITSIAS
60 70 80 90 100
NGGRVQCMQV WPPINKKKYE TLSYLPDLSQ EQLLSEVEYL LKNGWVPCLE
110 120 130 140 150
FETEHGFVYR ENNKSPGYYD GRYWTMWKLP MFGCTDATQV LAEVEEAKKA
160 170 180
YPQAWIRIIG FDNVRQVQCI SFIAYKPEGY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 87 – 88 | VE → PD AA sequence (Ref. 2) Curated | 2 | |
Sequence conflicti | 93 | N → D AA sequence (Ref. 2) Curated | 1 | |
Sequence conflicti | 153 | Q → E AA sequence (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02353 Genomic DNA Translation: CAA26208.1 M32419 Genomic DNA Translation: AAA34116.1 |
PIRi | A22934, RKNTSP |
RefSeqi | XP_016440853.1, XM_016585367.1 XP_016481946.1, XM_016626460.1 |
Genome annotation databases
GeneIDi | 107766567 107802883 |
KEGGi | nta:107766567 nta:107802883 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X02353 Genomic DNA Translation: CAA26208.1 M32419 Genomic DNA Translation: AAA34116.1 |
PIRi | A22934, RKNTSP |
RefSeqi | XP_016440853.1, XM_016585367.1 XP_016481946.1, XM_016626460.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EJ7 | X-ray | 2.45 | S | 58-180 | [»] | |
1RLC | X-ray | 2.70 | S | 58-180 | [»] | |
1RLD | X-ray | 2.50 | S/T | 58-180 | [»] | |
3RUB | X-ray | 2.00 | S | 58-180 | [»] | |
4RUB | X-ray | 2.70 | S/T/U/V | 58-180 | [»] | |
AlphaFoldDBi | P69249 | |||||
SMRi | P69249 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-40635N |
IntActi | P69249, 4 interactors |
MINTi | P69249 |
STRINGi | 4097.P69249 |
Proteomic databases
PRIDEi | P69249 |
Genome annotation databases
GeneIDi | 107766567 107802883 |
KEGGi | nta:107766567 nta:107802883 |
Phylogenomic databases
OMAi | KQCQVLS |
OrthoDBi | 1258997at2759 |
PhylomeDBi | P69249 |
Enzyme and pathway databases
BRENDAi | 4.1.1.39, 3645 |
SABIO-RKi | P69249 |
Miscellaneous databases
EvolutionaryTracei | P69249 |
Family and domain databases
CDDi | cd03527, RuBisCO_small, 1 hit |
Gene3Di | 3.30.190.10, 1 hit |
HAMAPi | MF_00859, RuBisCO_S_bact, 1 hit |
InterProi | View protein in InterPro IPR024681, RuBisCO_ssu IPR000894, RuBisCO_ssu_dom IPR024680, RuBisCO_ssu_N IPR036385, RuBisCO_ssu_sf |
PANTHERi | PTHR31262, PTHR31262, 1 hit |
Pfami | View protein in Pfam PF12338, RbcS, 1 hit PF00101, RuBisCO_small, 1 hit |
PRINTSi | PR00152, RUBISCOSMALL |
SMARTi | View protein in SMART SM00961, RuBisCO_small, 1 hit |
SUPFAMi | SSF55239, SSF55239, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RBS_TOBAC | |
Accessioni | P69249Primary (citable) accession number: P69249 Secondary accession number(s): P00866, P00867, P26666 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | May 25, 2022 | |
This is version 91 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families