UniProtKB - P68767 (AMPA_ECOLI)
Protein
Cytosol aminopeptidase
Gene
pepA
Organism
Escherichia coli (strain K12)
Status
Functioni
Probably involved in the processing and regular turnover of intracellular proteins (PubMed:20067529). Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Required for plasmid ColE1 site-specific recombination but not in its aminopeptidase activity. Could act as a structural component of the putative nucleoprotein complex in which the Xer recombination reaction takes place (PubMed:8057849).1 Publication1 Publication
Caution
The ligation for manganese is based on the ligation for zinc, an inhibitor, in the crystallographic structure reported in PubMed:10449417. The ligation for manganese in the active form of the enzyme may differ.Curated
Catalytic activityi
- Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. EC:3.4.11.1
- Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. EC:3.4.11.10
Cofactori
Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
Activity regulationi
Inhibited by zinc and EDTA.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 270 | Manganese 2Curated | 1 | |
Metal bindingi | 275 | Manganese 1Curated | 1 | |
Metal bindingi | 275 | Manganese 2Curated | 1 | |
Active sitei | 282 | Sequence analysis | 1 | |
Metal bindingi | 293 | Manganese 2Curated | 1 | |
Metal bindingi | 352 | Manganese 1Curated | 1 | |
Metal bindingi | 354 | Manganese 1Curated | 1 | |
Metal bindingi | 354 | Manganese 2Curated | 1 | |
Active sitei | 356 | Sequence analysis | 1 |
GO - Molecular functioni
- aminopeptidase activity Source: EcoliWiki
- DNA binding Source: EcoliWiki
- manganese ion binding Source: UniProtKB-UniRule
- metalloaminopeptidase activity Source: InterPro
- transcription antitermination factor activity, DNA binding Source: EcoliWiki
GO - Biological processi
- peptide catabolic process Source: EcoliWiki
- plasmid maintenance Source: EcoliWiki
- plasmid recombination Source: EcoliWiki
- transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Protease |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10694-MONOMER MetaCyc:EG10694-MONOMER |
Protein family/group databases
MEROPSi | M17.003 |
MoonProti | P68767 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pepA Synonyms:carP, xerB Ordered Locus Names:b4260, JW4217 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
A quadruple peptidase disruption (pepA, pepB, pepD and pepN) does not grow in M9 minimal medium, grows better when supplemented with casamino acids (PubMed:20067529).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 354 | E → A: Loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000165750 | 1 – 503 | Cytosol aminopeptidaseAdd BLAST | 503 |
Proteomic databases
jPOSTi | P68767 |
PaxDbi | P68767 |
PRIDEi | P68767 |
Interactioni
Subunit structurei
Homohexamer.
Protein-protein interaction databases
BioGRIDi | 4262726, 44 interactors |
DIPi | DIP-47860N |
IntActi | P68767, 5 interactors |
STRINGi | 511145.b4260 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P68767 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P68767 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase M17 family.Curated
Phylogenomic databases
eggNOGi | COG0260, Bacteria |
HOGENOMi | CLU_013734_2_2_6 |
InParanoidi | P68767 |
PhylomeDBi | P68767 |
Family and domain databases
CDDi | cd00433, Peptidase_M17, 1 hit |
Gene3Di | 3.40.220.10, 1 hit |
HAMAPi | MF_00181, Cytosol_peptidase_M17, 1 hit |
InterProi | View protein in InterPro IPR011356, Leucine_aapep/pepB IPR043472, Macro_dom-like IPR000819, Peptidase_M17_C IPR023042, Peptidase_M17_leu_NH2_pept IPR008283, Peptidase_M17_N |
PANTHERi | PTHR11963, PTHR11963, 1 hit |
Pfami | View protein in Pfam PF00883, Peptidase_M17, 1 hit PF02789, Peptidase_M17_N, 1 hit |
PRINTSi | PR00481, LAMNOPPTDASE |
SUPFAMi | SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS00631, CYTOSOL_AP, 1 hit |
i Sequence
Sequence statusi: Complete.
P68767-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEFSVKSGSP EKQRSACIVV GVFEPRRLSP IAEQLDKISD GYISALLRRG
60 70 80 90 100
ELEGKPGQTL LLHHVPNVLS ERILLIGCGK ERELDERQYK QVIQKTINTL
110 120 130 140 150
NDTGSMEAVC FLTELHVKGR NNYWKVRQAV ETAKETLYSF DQLKTNKSEP
160 170 180 190 200
RRPLRKMVFN VPTRRELTSG ERAIQHGLAI AAGIKAAKDL GNMPPNICNA
210 220 230 240 250
AYLASQARQL ADSYSKNVIT RVIGEQQMKE LGMHSYLAVG QGSQNESLMS
260 270 280 290 300
VIEYKGNASE DARPIVLVGK GLTFDSGGIS IKPSEGMDEM KYDMCGAAAV
310 320 330 340 350
YGVMRMVAEL QLPINVIGVL AGCENMPGGR AYRPGDVLTT MSGQTVEVLN
360 370 380 390 400
TDAEGRLVLC DVLTYVERFE PEAVIDVATL TGACVIALGH HITGLMANHN
410 420 430 440 450
PLAHELIAAS EQSGDRAWRL PLGDEYQEQL ESNFADMANI GGRPGGAITA
460 470 480 490 500
GCFLSRFTRK YNWAHLDIAG TAWRSGKAKG ATGRPVALLA QFLLNRAGFN
GEE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15130 Genomic DNA Translation: CAA33225.1 X86443 Genomic DNA Translation: CAA60164.1 U14003 Genomic DNA Translation: AAA97157.1 U00096 Genomic DNA Translation: AAC77217.1 AP009048 Genomic DNA Translation: BAE78257.1 |
PIRi | S04462, APECA |
RefSeqi | NP_418681.1, NC_000913.3 WP_000397144.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77217; AAC77217; b4260 BAE78257; BAE78257; BAE78257 |
GeneIDi | 58391074 948791 |
KEGGi | ecj:JW4217 eco:b4260 |
PATRICi | fig|1411691.4.peg.2444 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15130 Genomic DNA Translation: CAA33225.1 X86443 Genomic DNA Translation: CAA60164.1 U14003 Genomic DNA Translation: AAA97157.1 U00096 Genomic DNA Translation: AAC77217.1 AP009048 Genomic DNA Translation: BAE78257.1 |
PIRi | S04462, APECA |
RefSeqi | NP_418681.1, NC_000913.3 WP_000397144.1, NZ_STEB01000013.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GYT | X-ray | 2.50 | A/B/C/D/E/F/G/H/I/J/K/L | 1-503 | [»] | |
SMRi | P68767 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262726, 44 interactors |
DIPi | DIP-47860N |
IntActi | P68767, 5 interactors |
STRINGi | 511145.b4260 |
Protein family/group databases
MEROPSi | M17.003 |
MoonProti | P68767 |
Proteomic databases
jPOSTi | P68767 |
PaxDbi | P68767 |
PRIDEi | P68767 |
Genome annotation databases
EnsemblBacteriai | AAC77217; AAC77217; b4260 BAE78257; BAE78257; BAE78257 |
GeneIDi | 58391074 948791 |
KEGGi | ecj:JW4217 eco:b4260 |
PATRICi | fig|1411691.4.peg.2444 |
Organism-specific databases
EchoBASEi | EB0688 |
Phylogenomic databases
eggNOGi | COG0260, Bacteria |
HOGENOMi | CLU_013734_2_2_6 |
InParanoidi | P68767 |
PhylomeDBi | P68767 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10694-MONOMER MetaCyc:EG10694-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P68767 |
PROi | PR:P68767 |
Family and domain databases
CDDi | cd00433, Peptidase_M17, 1 hit |
Gene3Di | 3.40.220.10, 1 hit |
HAMAPi | MF_00181, Cytosol_peptidase_M17, 1 hit |
InterProi | View protein in InterPro IPR011356, Leucine_aapep/pepB IPR043472, Macro_dom-like IPR000819, Peptidase_M17_C IPR023042, Peptidase_M17_leu_NH2_pept IPR008283, Peptidase_M17_N |
PANTHERi | PTHR11963, PTHR11963, 1 hit |
Pfami | View protein in Pfam PF00883, Peptidase_M17, 1 hit PF02789, Peptidase_M17_N, 1 hit |
PRINTSi | PR00481, LAMNOPPTDASE |
SUPFAMi | SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS00631, CYTOSOL_AP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPA_ECOLI | |
Accessioni | P68767Primary (citable) accession number: P68767 Secondary accession number(s): P11648, Q2M649 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | December 21, 2004 | |
Last modified: | April 7, 2021 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families