UniProtKB - P68688 (GLRX1_ECOLI)
Protein
Glutaredoxin 1
Gene
grxA
Organism
Escherichia coli (strain K12)
Status
Functioni
The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing some disulfide bonds in a coupled system with glutathione reductase.
GO - Molecular functioni
- electron transfer activity Source: InterPro
- nucleotide binding Source: UniProtKB-KW
- protein disulfide oxidoreductase activity Source: InterPro
GO - Biological processi
- cell redox homeostasis Source: InterPro
- deoxyribonucleotide biosynthetic process Source: UniProtKB-KW
Keywordsi
Biological process | Deoxyribonucleotide synthesis, Electron transport, Transport |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:RED-GLUTAREDOXIN MetaCyc:RED-GLUTAREDOXIN |
SABIO-RKi | P68688 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutaredoxin 1Short name: Grx1 |
Gene namesi | Name:grxA Synonyms:grx Ordered Locus Names:b0849, JW0833 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | G → A: No defect in AMPylation by YdiU. 1 Publication | 1 | |
Mutagenesisi | 11 | C → A: Abolishes AMPylation by YdiU. 1 Publication | 1 | |
Mutagenesisi | 12 | P → A: Abolishes AMPylation by YdiU. 1 Publication | 1 | |
Mutagenesisi | 13 | Y → F, S or T: Abolishes AMPylation by YdiU. 1 Publication | 1 | |
Mutagenesisi | 14 | C → A: Partially abolishes AMPylation by YdiU. 1 Publication | 1 | |
Mutagenesisi | 15 | V → A: Partially abolishes AMPylation by YdiU. 1 Publication | 1 | |
Mutagenesisi | 16 | R → A: Partially abolishes AMPylation by YdiU. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000141581 | 1 – 85 | Glutaredoxin 1Add BLAST | 85 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 11 ↔ 14 | Redox-active | ||
Modified residuei | 13 | O-AMP-tyrosine; by YdiU1 Publication | 1 |
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
jPOSTi | P68688 |
PaxDbi | P68688 |
PRIDEi | P68688 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4259990, 8 interactors 849853, 5 interactors |
IntActi | P68688, 16 interactors |
STRINGi | 511145.b0849 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P68688 |
SMRi | P68688 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P68688 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 85 | GlutaredoxinPROSITE-ProRule annotationAdd BLAST | 85 |
Sequence similaritiesi
Belongs to the glutaredoxin family.Curated
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG0695, Bacteria |
InParanoidi | P68688 |
PhylomeDBi | P68688 |
Family and domain databases
DisProti | DP01740 |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR011767, GLR_AS IPR002109, Glutaredoxin IPR014025, Glutaredoxin_subgr IPR011902, GRXA IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00462, Glutaredoxin, 1 hit |
PRINTSi | PR00160, GLUTAREDOXIN |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR02183, GRXA, 1 hit |
PROSITEi | View protein in PROSITE PS00195, GLUTAREDOXIN_1, 1 hit PS51354, GLUTAREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P68688-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQTVIFGRSG CPYCVRAKDL AEKLSNERDD FQYQYVDIRA EGITKEDLQQ
60 70 80
KAGKPVETVP QIFVDQQHIG GYTDFAAWVK ENLDA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13449 Genomic DNA Translation: AAA23936.1 U18655 Genomic DNA Translation: AAC43449.1 U00096 Genomic DNA Translation: AAC73936.1 AP009048 Genomic DNA Translation: BAA35552.1 |
PIRi | A00283, GDEC |
RefSeqi | NP_415370.1, NC_000913.3 WP_001195240.1, NZ_STEB01000019.1 |
Genome annotation databases
EnsemblBacteriai | AAC73936; AAC73936; b0849 BAA35552; BAA35552; BAA35552 |
GeneIDi | 58388701 945479 |
KEGGi | ecj:JW0833 eco:b0849 |
PATRICi | fig|1411691.4.peg.1429 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13449 Genomic DNA Translation: AAA23936.1 U18655 Genomic DNA Translation: AAC43449.1 U00096 Genomic DNA Translation: AAC73936.1 AP009048 Genomic DNA Translation: BAA35552.1 |
PIRi | A00283, GDEC |
RefSeqi | NP_415370.1, NC_000913.3 WP_001195240.1, NZ_STEB01000019.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EGO | NMR | - | A | 1-85 | [»] | |
1EGR | NMR | - | A | 1-85 | [»] | |
1GRX | NMR | - | A | 1-85 | [»] | |
1QFN | NMR | - | A | 1-85 | [»] | |
1UPY | model | - | G | 1-85 | [»] | |
1UPZ | model | - | G | 1-85 | [»] | |
1UQ0 | model | - | G | 1-85 | [»] | |
1UQ1 | model | - | G | 1-85 | [»] | |
1UQ2 | model | - | G | 1-85 | [»] | |
1UQ3 | model | - | G | 1-85 | [»] | |
1UQ6 | model | - | G | 1-85 | [»] | |
1UQ7 | model | - | G | 1-85 | [»] | |
1UQH | model | - | G | 1-85 | [»] | |
1UQN | model | - | G | 1-85 | [»] | |
BMRBi | P68688 | |||||
SMRi | P68688 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259990, 8 interactors 849853, 5 interactors |
IntActi | P68688, 16 interactors |
STRINGi | 511145.b0849 |
Proteomic databases
jPOSTi | P68688 |
PaxDbi | P68688 |
PRIDEi | P68688 |
Genome annotation databases
EnsemblBacteriai | AAC73936; AAC73936; b0849 BAA35552; BAA35552; BAA35552 |
GeneIDi | 58388701 945479 |
KEGGi | ecj:JW0833 eco:b0849 |
PATRICi | fig|1411691.4.peg.1429 |
Organism-specific databases
EchoBASEi | EB0412 |
Phylogenomic databases
eggNOGi | COG0695, Bacteria |
InParanoidi | P68688 |
PhylomeDBi | P68688 |
Enzyme and pathway databases
BioCyci | EcoCyc:RED-GLUTAREDOXIN MetaCyc:RED-GLUTAREDOXIN |
SABIO-RKi | P68688 |
Miscellaneous databases
EvolutionaryTracei | P68688 |
PROi | PR:P68688 |
Family and domain databases
DisProti | DP01740 |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR011767, GLR_AS IPR002109, Glutaredoxin IPR014025, Glutaredoxin_subgr IPR011902, GRXA IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF00462, Glutaredoxin, 1 hit |
PRINTSi | PR00160, GLUTAREDOXIN |
SUPFAMi | SSF52833, SSF52833, 1 hit |
TIGRFAMsi | TIGR02183, GRXA, 1 hit |
PROSITEi | View protein in PROSITE PS00195, GLUTAREDOXIN_1, 1 hit PS51354, GLUTAREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLRX1_ECOLI | |
Accessioni | P68688Primary (citable) accession number: P68688 Secondary accession number(s): P00277 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families