We will be switching to the new UniProt website soon. Please explore and share your feedback.
Take me to the new website.
UniProtKB - P68398 (TADA_ECOLI)
Protein
tRNA-specific adenosine deaminase
Gene
tadA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2). Essential for cell viability.
UniRule annotation2 PublicationsCatalytic activityi
- EC:3.5.4.33UniRule annotation2 Publications
Cofactori
Zn2+UniRule annotation1 PublicationNote: Binds 1 zinc ion per subunit.UniRule annotation1 Publication
Kineticsi
kcat is 13 min(-1).1 Publication
- KM=0.83 µM for tRNA(Arg2)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 57 | Zinc; via pros nitrogen; catalyticUniRule annotation1 Publication | 1 | |
Active sitei | 59 | Proton donor1 Publication | 1 | |
Metal bindingi | 87 | Zinc; catalyticUniRule annotation1 Publication | 1 | |
Metal bindingi | 90 | Zinc; catalyticUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- tRNA-specific adenosine-34 deaminase activity Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- adenosine to inosine editing Source: EcoCyc
- tRNA wobble adenosine to inosine editing Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Biological process | tRNA processing |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11372-MONOMER |
BRENDAi | 3.5.4.33, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA-specific adenosine deaminaseUniRule annotation (EC:3.5.4.33UniRule annotation) |
Gene namesi | Name:tadAUniRule annotation Synonyms:yfhC Ordered Locus Names:b2559, JW2543 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Mutation makes E.coli resistant to the toxic proteins encoded by the gef gene family.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 53 | D → E: Resistance to the cell-killing function encoded by the gef gene family. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171733 | 1 – 167 | tRNA-specific adenosine deaminaseAdd BLAST | 167 |
Proteomic databases
jPOSTi | P68398 |
PaxDbi | P68398 |
PRIDEi | P68398 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261245, 59 interactors |
IntActi | P68398, 4 interactors |
STRINGi | 511145.b2559 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P68398 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P68398 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 117 | CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST | 112 |
Sequence similaritiesi
Belongs to the cytidine and deoxycytidylate deaminase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0590, Bacteria |
HOGENOMi | CLU_025810_3_0_6 |
InParanoidi | P68398 |
PhylomeDBi | P68398 |
Family and domain databases
HAMAPi | MF_00972, tRNA_aden_deaminase, 1 hit |
InterProi | View protein in InterPro IPR016192, APOBEC/CMP_deaminase_Zn-bd IPR002125, CMP_dCMP_dom IPR016193, Cytidine_deaminase-like IPR028883, tRNA_aden_deaminase |
Pfami | View protein in Pfam PF14437, MafB19-deam, 1 hit |
SUPFAMi | SSF53927, SSF53927, 1 hit |
PROSITEi | View protein in PROSITE PS00903, CYT_DCMP_DEAMINASES_1, 1 hit PS51747, CYT_DCMP_DEAMINASES_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P68398-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEVEFSHEY WMRHALTLAK RAWDEREVPV GAVLVHNNRV IGEGWNRPIG
60 70 80 90 100
RHDPTAHAEI MALRQGGLVM QNYRLIDATL YVTLEPCVMC AGAMIHSRIG
110 120 130 140 150
RVVFGARDAK TGAAGSLMDV LHHPGMNHRV EITEGILADE CAALLSDFFR
160
MRRQEIKAQK KAQSSTD
Sequence cautioni
The sequence AAA79821 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAA10909 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAE76735 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence CAA51064 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72336 Genomic DNA Translation: CAA51064.1 Different initiation. D64044 Genomic DNA Translation: BAA10909.1 Different initiation. U36841 Genomic DNA Translation: AAA79821.1 Different initiation. U00096 Genomic DNA Translation: AAC75612.2 AP009048 Genomic DNA Translation: BAE76735.1 Different initiation. |
PIRi | F65033 |
RefSeqi | NP_417054.2, NC_000913.3 WP_001297409.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75612; AAC75612; b2559 BAE76735; BAE76735; BAE76735 |
GeneIDi | 66673553 947027 |
KEGGi | ecj:JW2543 eco:b2559 |
PATRICi | fig|1411691.4.peg.4175 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X72336 Genomic DNA Translation: CAA51064.1 Different initiation. D64044 Genomic DNA Translation: BAA10909.1 Different initiation. U36841 Genomic DNA Translation: AAA79821.1 Different initiation. U00096 Genomic DNA Translation: AAC75612.2 AP009048 Genomic DNA Translation: BAE76735.1 Different initiation. |
PIRi | F65033 |
RefSeqi | NP_417054.2, NC_000913.3 WP_001297409.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Z3A | X-ray | 2.03 | A/B | 2-167 | [»] | |
6VPC | electron microscopy | 3.20 | E/F | 1-167 | [»] | |
SMRi | P68398 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261245, 59 interactors |
IntActi | P68398, 4 interactors |
STRINGi | 511145.b2559 |
Proteomic databases
jPOSTi | P68398 |
PaxDbi | P68398 |
PRIDEi | P68398 |
Genome annotation databases
EnsemblBacteriai | AAC75612; AAC75612; b2559 BAE76735; BAE76735; BAE76735 |
GeneIDi | 66673553 947027 |
KEGGi | ecj:JW2543 eco:b2559 |
PATRICi | fig|1411691.4.peg.4175 |
Organism-specific databases
EchoBASEi | EB1346 |
Phylogenomic databases
eggNOGi | COG0590, Bacteria |
HOGENOMi | CLU_025810_3_0_6 |
InParanoidi | P68398 |
PhylomeDBi | P68398 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11372-MONOMER |
BRENDAi | 3.5.4.33, 2026 |
Miscellaneous databases
EvolutionaryTracei | P68398 |
PROi | PR:P68398 |
Family and domain databases
HAMAPi | MF_00972, tRNA_aden_deaminase, 1 hit |
InterProi | View protein in InterPro IPR016192, APOBEC/CMP_deaminase_Zn-bd IPR002125, CMP_dCMP_dom IPR016193, Cytidine_deaminase-like IPR028883, tRNA_aden_deaminase |
Pfami | View protein in Pfam PF14437, MafB19-deam, 1 hit |
SUPFAMi | SSF53927, SSF53927, 1 hit |
PROSITEi | View protein in PROSITE PS00903, CYT_DCMP_DEAMINASES_1, 1 hit PS51747, CYT_DCMP_DEAMINASES_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TADA_ECOLI | |
Accessioni | P68398Primary (citable) accession number: P68398 Secondary accession number(s): P30134, Q2MAH1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 23, 2004 |
Last sequence update: | June 26, 2007 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families