UniProtKB - P68368 (TBA4A_MOUSE)
Tubulin alpha-4A chain
Tuba4a
Functioni
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Miscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 142 – 148 | GTPSequence analysis | 7 |
GO - Molecular functioni
- enzyme binding Source: UniProtKB
- GTPase activity Source: InterPro
- GTP binding Source: GO_Central
- protein kinase binding Source: MGI
- structural constituent of cytoskeleton Source: GO_Central
GO - Biological processi
- microtubule cytoskeleton organization Source: GO_Central
- mitotic cell cycle Source: GO_Central
Keywordsi
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-190840, Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane R-MMU-2132295, MHC class II antigen presentation R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-437239, Recycling pathway of L1 R-MMU-5610787, Hedgehog 'off' state R-MMU-5617833, Cilium Assembly R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-5620924, Intraflagellar transport R-MMU-5663220, RHO GTPases Activate Formins R-MMU-6807878, COPI-mediated anterograde transport R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-6811436, COPI-independent Golgi-to-ER retrograde traffic R-MMU-68877, Mitotic Prometaphase R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854518, AURKA Activation by TPX2 R-MMU-8955332, Carboxyterminal post-translational modifications of tubulin R-MMU-9646399, Aggrephagy R-MMU-9648025, EML4 and NUDC in mitotic spindle formation R-MMU-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III R-MMU-983189, Kinesins |
Names & Taxonomyi
Protein namesi | Recommended name: Tubulin alpha-4A chainAlternative name(s): Alpha-tubulin 4 Alpha-tubulin isotype M-alpha-4 Tubulin alpha-4 chain |
Gene namesi | Name:Tuba4a Synonyms:Tuba4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1095410, Tuba4a |
VEuPathDBi | HostDB:ENSMUSG00000026202 |
Subcellular locationi
Cytoskeleton
Cytoskeleton
- cytoskeleton Source: MGI
- microtubule Source: MGI
- microtubule cytoskeleton Source: MGI
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, MicrotubulePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048123 | 1 – 448 | Tubulin alpha-4A chainAdd BLAST | 448 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 40 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 48 | PhosphoserineBy similarity | 1 | |
Modified residuei | 83 | 3'-nitrotyrosineCombined sources | 1 | |
Modified residuei | 432 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 439 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, Nitration, PhosphoproteinProteomic databases
EPDi | P68368 |
jPOSTi | P68368 |
MaxQBi | P68368 |
PaxDbi | P68368 |
PeptideAtlasi | P68368 |
PRIDEi | P68368 |
ProteomicsDBi | 262937 |
PTM databases
CarbonylDBi | P68368 |
iPTMneti | P68368 |
PhosphoSitePlusi | P68368 |
SwissPalmi | P68368 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026202, Expressed in brain and 312 other tissues |
Genevisiblei | P68368, MM |
Interactioni
Subunit structurei
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Interacts with CFAP157 (PubMed:27965440).
1 PublicationGO - Molecular functioni
- enzyme binding Source: UniProtKB
- protein kinase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204375, 47 interactors |
IntActi | P68368, 17 interactors |
MINTi | P68368 |
STRINGi | 10090.ENSMUSP00000140657 |
Miscellaneous databases
RNActi | P68368, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1 – 4 | MREC motifBy similarity | 4 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1376, Eukaryota |
GeneTreei | ENSGT00950000183165 |
HOGENOMi | CLU_015718_0_0_1 |
InParanoidi | P68368 |
OMAi | ICHLHIG |
OrthoDBi | 514396at2759 |
PhylomeDBi | P68368 |
TreeFami | TF300314 |
Family and domain databases
Gene3Di | 1.10.287.600, 1 hit 3.30.1330.20, 1 hit 3.40.50.1440, 1 hit |
InterProi | View protein in InterPro IPR002452, Alpha_tubulin IPR008280, Tub_FtsZ_C IPR000217, Tubulin IPR037103, Tubulin/FtsZ-like_C IPR018316, Tubulin/FtsZ_2-layer-sand-dom IPR036525, Tubulin/FtsZ_GTPase_sf IPR023123, Tubulin_C IPR017975, Tubulin_CS IPR003008, Tubulin_FtsZ_GTPase |
PANTHERi | PTHR11588, PTHR11588, 1 hit |
Pfami | View protein in Pfam PF00091, Tubulin, 1 hit PF03953, Tubulin_C, 1 hit |
PRINTSi | PR01162, ALPHATUBULIN PR01161, TUBULIN |
SMARTi | View protein in SMART SM00864, Tubulin, 1 hit SM00865, Tubulin_C, 1 hit |
SUPFAMi | SSF52490, SSF52490, 1 hit SSF55307, SSF55307, 1 hit |
PROSITEi | View protein in PROSITE PS00227, TUBULIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MRECISVHVG QAGVQMGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFT
60 70 80 90 100
TFFCETGAGK HVPRAVFVDL EPTVIDEIRN GPYRQLFHPE QLITGKEDAA
110 120 130 140 150
NNYARGHYTI GKEIIDPVLD RIRKLSDQCT GLQGFLVFHS FGGGTGSGFT
160 170 180 190 200
SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC
210 220 230 240 250
AFMVDNEAIY DICRRNLDIE RPTYTNLNRL ISQIVSSITA SLRFDGALNV
260 270 280 290 300
DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN
310 320 330 340 350
QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIAAIKTKRS IQFVDWCPTG
360 370 380 390 400
FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA
410 420 430 440
KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGIDSY EDEDEGEE
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MQA5 | A0A0A0MQA5_MOUSE | Tubulin alpha chain | Tuba4a | 477 | Annotation score: | ||
A0A087WRB4 | A0A087WRB4_MOUSE | Tubulin alpha-4A chain | Tuba4a | 100 | Annotation score: | ||
A0A087WSB0 | A0A087WSB0_MOUSE | Tubulin alpha-4A chain | Tuba4a | 81 | Annotation score: | ||
A0A087WSL5 | A0A087WSL5_MOUSE | Tubulin alpha-4A chain | Tuba4a | 89 | Annotation score: | ||
A0A087WS35 | A0A087WS35_MOUSE | Tubulin alpha-4A chain | Tuba4a | 75 | Annotation score: | ||
A0A087WQS4 | A0A087WQS4_MOUSE | Tubulin alpha-4A chain | Tuba4a | 125 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 | M → G in BAE34732 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13444 mRNA Translation: AAA40502.1 AK002427 mRNA Translation: BAB22094.1 AK078355 mRNA Translation: BAC37234.1 AK158934 mRNA Translation: BAE34732.1 Different initiation. BC019959 mRNA Translation: AAH19959.1 |
CCDSi | CCDS35623.1 |
PIRi | I77427 |
RefSeqi | NP_033473.1, NM_009447.4 |
Genome annotation databases
Ensembli | ENSMUST00000186213; ENSMUSP00000140657; ENSMUSG00000026202 |
GeneIDi | 22145 |
KEGGi | mmu:22145 |
UCSCi | uc007bok.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M13444 mRNA Translation: AAA40502.1 AK002427 mRNA Translation: BAB22094.1 AK078355 mRNA Translation: BAC37234.1 AK158934 mRNA Translation: BAE34732.1 Different initiation. BC019959 mRNA Translation: AAH19959.1 |
CCDSi | CCDS35623.1 |
PIRi | I77427 |
RefSeqi | NP_033473.1, NM_009447.4 |
3D structure databases
AlphaFoldDBi | P68368 |
SMRi | P68368 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204375, 47 interactors |
IntActi | P68368, 17 interactors |
MINTi | P68368 |
STRINGi | 10090.ENSMUSP00000140657 |
PTM databases
CarbonylDBi | P68368 |
iPTMneti | P68368 |
PhosphoSitePlusi | P68368 |
SwissPalmi | P68368 |
Proteomic databases
EPDi | P68368 |
jPOSTi | P68368 |
MaxQBi | P68368 |
PaxDbi | P68368 |
PeptideAtlasi | P68368 |
PRIDEi | P68368 |
ProteomicsDBi | 262937 |
Protocols and materials databases
Antibodypediai | 3138, 503 antibodies from 38 providers |
DNASUi | 22145 |
Genome annotation databases
Ensembli | ENSMUST00000186213; ENSMUSP00000140657; ENSMUSG00000026202 |
GeneIDi | 22145 |
KEGGi | mmu:22145 |
UCSCi | uc007bok.1, mouse |
Organism-specific databases
CTDi | 7277 |
MGIi | MGI:1095410, Tuba4a |
VEuPathDBi | HostDB:ENSMUSG00000026202 |
Phylogenomic databases
eggNOGi | KOG1376, Eukaryota |
GeneTreei | ENSGT00950000183165 |
HOGENOMi | CLU_015718_0_0_1 |
InParanoidi | P68368 |
OMAi | ICHLHIG |
OrthoDBi | 514396at2759 |
PhylomeDBi | P68368 |
TreeFami | TF300314 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-190840, Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane R-MMU-2132295, MHC class II antigen presentation R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-3371497, HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-437239, Recycling pathway of L1 R-MMU-5610787, Hedgehog 'off' state R-MMU-5617833, Cilium Assembly R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-5620924, Intraflagellar transport R-MMU-5663220, RHO GTPases Activate Formins R-MMU-6807878, COPI-mediated anterograde transport R-MMU-6811434, COPI-dependent Golgi-to-ER retrograde traffic R-MMU-6811436, COPI-independent Golgi-to-ER retrograde traffic R-MMU-68877, Mitotic Prometaphase R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854518, AURKA Activation by TPX2 R-MMU-8955332, Carboxyterminal post-translational modifications of tubulin R-MMU-9646399, Aggrephagy R-MMU-9648025, EML4 and NUDC in mitotic spindle formation R-MMU-9668328, Sealing of the nuclear envelope (NE) by ESCRT-III R-MMU-983189, Kinesins |
Miscellaneous databases
BioGRID-ORCSi | 22145, 2 hits in 73 CRISPR screens |
ChiTaRSi | Tuba4a, mouse |
PROi | PR:P68368 |
RNActi | P68368, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026202, Expressed in brain and 312 other tissues |
Genevisiblei | P68368, MM |
Family and domain databases
Gene3Di | 1.10.287.600, 1 hit 3.30.1330.20, 1 hit 3.40.50.1440, 1 hit |
InterProi | View protein in InterPro IPR002452, Alpha_tubulin IPR008280, Tub_FtsZ_C IPR000217, Tubulin IPR037103, Tubulin/FtsZ-like_C IPR018316, Tubulin/FtsZ_2-layer-sand-dom IPR036525, Tubulin/FtsZ_GTPase_sf IPR023123, Tubulin_C IPR017975, Tubulin_CS IPR003008, Tubulin_FtsZ_GTPase |
PANTHERi | PTHR11588, PTHR11588, 1 hit |
Pfami | View protein in Pfam PF00091, Tubulin, 1 hit PF03953, Tubulin_C, 1 hit |
PRINTSi | PR01162, ALPHATUBULIN PR01161, TUBULIN |
SMARTi | View protein in SMART SM00864, Tubulin, 1 hit SM00865, Tubulin_C, 1 hit |
SUPFAMi | SSF52490, SSF52490, 1 hit SSF55307, SSF55307, 1 hit |
PROSITEi | View protein in PROSITE PS00227, TUBULIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TBA4A_MOUSE | |
Accessioni | P68368Primary (citable) accession number: P68368 Secondary accession number(s): P05215, Q3TY31 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | May 25, 2022 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families