UniProtKB - P68315 (RP07_VACCC)
DNA-directed RNA polymerase 7 kDa subunit
RPO7
Functioni
Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of D6 and A7, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase.
Catalytic activityi
- EC:2.7.7.6
GO - Molecular functioni
- DNA binding Source: InterPro
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Transcription |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RPO7 ORF Names:G5.5R |
Organismi | Vaccinia virus (strain Copenhagen) (VACV) |
Taxonomic identifieri | 10249 [NCBI] |
Taxonomic lineagei | Viruses › Varidnaviria › Bamfordvirae › Nucleocytoviricota › Pokkesviricetes › Chitovirales › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Proteomesi |
|
Subcellular locationi
- Virion Curated Note: All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome. This is necessary because viral early mRNAs are synthesized within minutes after virus entry into the cell and are extruded through pores in the core particle.
Keywords - Cellular componenti
DNA-directed RNA polymerase, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000099156 | 1 – 63 | DNA-directed RNA polymerase 7 kDa subunitAdd BLAST | 63 |
Expressioni
Inductioni
Keywords - Developmental stagei
Early proteinInteractioni
Subunit structurei
The DNA-dependent RNA polymerase used for intermediate and late genes expression consists of eight subunits 147 kDa, 133 kDa, 35 kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500 kDa in mass. The same holoenzyme, with the addition of the transcription-specificity factor RAP94, is used for early gene expression (By similarity).
By similarityFamily & Domainsi
Sequence similaritiesi
Family and domain databases
InterProi | View protein in InterPro IPR008448, RNA_pol_7kDa_chordopoxvir |
Pfami | View protein in Pfam PF05864, Chordopox_RPO7, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVFQLVCSTC GKDISHERYK LIIRKKSLKD VLVSVKNECC RLKLSTQIEP
60
QRNLTVQPLL DIN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35027 Genomic DNA Translation: AAB59816.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M35027 Genomic DNA Translation: AAB59816.1 |
3D structure databases
SMRi | P68315 |
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR008448, RNA_pol_7kDa_chordopoxvir |
Pfami | View protein in Pfam PF05864, Chordopox_RPO7, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RP07_VACCC | |
Accessioni | P68315Primary (citable) accession number: P68315 Secondary accession number(s): Q89560 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 9, 2004 |
Last sequence update: | November 9, 2004 | |
Last modified: | September 29, 2021 | |
This is version 54 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families