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Protein

14-3-3 protein theta

Gene

Ywhaq

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • negative regulation of ion transmembrane transport Source: MGI
  • negative regulation of transcription, DNA-templated Source: MGI
  • protein targeting Source: MGI
  • signal transduction Source: MGI
  • small GTPase mediated signal transduction Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-111447 Activation of BAD and translocation to mitochondria
R-MMU-1445148 Translocation of GLUT4 to the plasma membrane
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein theta
Alternative name(s):
14-3-3 protein tau
Gene namesi
Name:Ywhaq
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:891963 Ywhaq

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586371 – 24514-3-3 protein thetaAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3N6-acetyllysineBy similarity1
Modified residuei49N6-acetyllysine; alternateBy similarity1
Cross-linki49Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei68N6-acetyllysineBy similarity1
Modified residuei82Nitrated tyrosineBy similarity1
Modified residuei92PhosphoserineCombined sources1
Modified residuei104Nitrated tyrosineBy similarity1
Modified residuei115N6-acetyllysineBy similarity1
Modified residuei232Phosphoserine; by CK1By similarityCurated1

Keywords - PTMi

Acetylation, Isopeptide bond, Nitration, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP68254
PaxDbiP68254
PeptideAtlasiP68254
PRIDEiP68254

2D gel databases

REPRODUCTION-2DPAGEiP68254

PTM databases

iPTMnetiP68254
PhosphoSitePlusiP68254
SwissPalmiP68254

Expressioni

Gene expression databases

BgeeiENSMUSG00000076432
ExpressionAtlasiP68254 baseline and differential
GenevisibleiP68254 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with SSH1 (By similarity). Interacts with GAB2. Interacts with RGS7 (phosphorylated form) (By similarity). Interacts with CDK16 (PubMed:9197417). Interacts with the 'Ser-241' phosphorylated form of PDPK1 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with SLITRK1 (By similarity). Interacts with RIPOR2 (By similarity). Interacts with INAVA; the interaction increases upon PRR (pattern recognition receptor) stimulation and is required for cellular signaling pathway activation and cytokine secretion (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei56Interaction with phosphoserine on interacting proteinBy similarity1
Sitei127Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204622, 15 interactors
CORUMiP68254
ELMiP68254
IntActiP68254, 23 interactors
MINTiP68254
STRINGi10090.ENSMUSP00000100067

Structurei

3D structure databases

ProteinModelPortaliP68254
SMRiP68254
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
HOVERGENiHBG050423
InParanoidiP68254
KOiK16197
PhylomeDBiP68254
TreeFamiTF102002

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P68254-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKTELIQKA KLAEQAERYD DMATCMKAVT EQGAELSNEE RNLLSVAYKN
60 70 80 90 100
VVGGRRSAWR VISSIEQKTD TSDKKLQLIK DYREKVESEL RSICTTVLEL
110 120 130 140 150
LDKYLIANAT NPESKVFYLK MKGDYFRYLA EVACGDDRKQ TIENSQGAYQ
160 170 180 190 200
EAFDISKKEM QPTHPIRLGL ALNFSVFYYE ILNNPELACT LAKTAFDEAI
210 220 230 240
AELDTLNEDS YKDSTLIMQL LRDNLTLWTS DSAGEECDAA EGAEN
Length:245
Mass (Da):27,778
Last modified:October 25, 2004 - v1
Checksum:iE30471260E8B366E
GO
Isoform 2 (identifier: P68254-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     227-245: LWTSDSAGEECDAAEGAEN → FTCVELETVSVCFSLLS

Note: No experimental confirmation available.
Show »
Length:243
Mass (Da):27,701
Checksum:iE03CA8AFF03BA947
GO

Sequence cautioni

The sequence AAH80802 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH85299 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26M → I in BAE35397 (PubMed:16141072).Curated1
Sequence conflicti227L → S in BAE27120 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016340227 – 245LWTSD…EGAEN → FTCVELETVSVCFSLLS in isoform 2. 1 PublicationAdd BLAST19

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57312 mRNA Translation: AAC53257.1
U56243 mRNA Translation: AAB72023.1
D87662 mRNA Translation: BAA13423.1
AK145568 mRNA Translation: BAE26517.1
AK146371 mRNA Translation: BAE27120.1
AK150850 mRNA Translation: BAE29907.1
AK151437 mRNA Translation: BAE30400.1
AK151715 mRNA Translation: BAE30634.1
AK151864 mRNA Translation: BAE30752.1
AK159403 mRNA Translation: BAE35055.1
AK159817 mRNA Translation: BAE35397.1
AK163237 mRNA Translation: BAE37249.1
AK167397 mRNA Translation: BAE39486.1
AL929409 Genomic DNA No translation available.
BC080802 mRNA Translation: AAH80802.1 Different initiation.
BC085299 mRNA Translation: AAH85299.1 Different initiation.
BC090838 mRNA Translation: AAH90838.1
BC106164 mRNA Translation: AAI06165.1
CCDSiCCDS25837.1 [P68254-1]
RefSeqiNP_035869.1, NM_011739.3 [P68254-1]
UniGeneiMm.289630

Genome annotation databases

EnsembliENSMUST00000049531; ENSMUSP00000106602; ENSMUSG00000076432 [P68254-1]
ENSMUST00000103002; ENSMUSP00000100067; ENSMUSG00000076432 [P68254-1]
GeneIDi22630
KEGGimmu:22630
UCSCiuc007ndw.1 mouse [P68254-1]
uc007ndx.1 mouse [P68254-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei1433T_MOUSE
AccessioniPrimary (citable) accession number: P68254
Secondary accession number(s): P35216
, Q3TW69, Q3UJN5, Q5SP76, Q5U423, Q66JR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: June 20, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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