YWHAB

Functionⁱ

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.By similarity1 Publication

GO - Molecular functionⁱ

protein domain specific binding Source: AgBase

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

negative regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
protein targeting Source: AgBase

View the complete GO annotation on QuickGO ...

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 14-3-3 protein beta/alpha Alternative name(s): Protein kinase C inhibitor protein 1 Short name: KCIP-1 Cleaved into the following chain: 14-3-3 protein beta/alpha, N-terminally processed
Gene namesⁱ	Name:YWHAB
Organismⁱ	Bos taurus (Bovine)
Taxonomic identifierⁱ	9913 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos
Proteomesⁱ	UP000009136 Componentⁱ: Unplaced

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Length
ChainⁱPRO_0000367899	1 – 246	14-3-3 protein beta/alphaAdd BLAST	246
Initiator methionineⁱ		Removed; alternateBy similarity1 Publication
ChainⁱPRO_0000000001	2 – 246	14-3-3 protein beta/alpha, N-terminally processedAdd BLAST	245

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	1	N-acetylmethionineBy similarity	1
Modified residueⁱ	2	N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processedBy similarity	1
Modified residueⁱ	2	PhosphothreonineBy similarity	1
Modified residueⁱ	5	N6-acetyllysineBy similarity	1
Modified residueⁱ	51	N6-acetyllysine; alternateBy similarity	1
Cross-linkⁱ	51	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residueⁱ	60	PhosphoserineBy similarity	1
Modified residueⁱ	70	N6-acetyllysineBy similarity	1
Modified residueⁱ	84	Nitrated tyrosineBy similarity	1
Modified residueⁱ	106	Nitrated tyrosineBy similarity	1
Modified residueⁱ	117	N6-acetyllysineBy similarity	1
Modified residueⁱ	186	PhosphoserineBy similarity	1
Modified residueⁱ	232	PhosphoserineBy similarity	1
Isoform Short (identifier: P68250-2)
Modified residueⁱ	1	N-acetylmethionineBy similarity	1

Post-translational modificationⁱ

The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.By similarity

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Nitration, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbⁱ	P68250
PeptideAtlas More...PeptideAtlasⁱ	P68250
PRIDEⁱ	P68250

PTM databases

iPTMnetⁱ	P68250

iPTMnetⁱ

P68250

Interactionⁱ

Subunit structureⁱ

Homodimer (By similarity). Interacts with SAMSN1 and PRKCE (By similarity). Interacts with AKAP13. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2. Interacts with YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with PKA-phosphorylated AANAT. Interacts with MYO1C. Interacts with SIRT2 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity). Interacts (via phosphorylated form) with YWHAB; this interaction occurs in a protein kinase AKT1-dependent manner (By similarity). Interacts with SLITRK1. Interacts with SYNPO2 (phosphorylated form); YWHAB competes with ACTN2 for interaction with SYNPO2 (By similarity). Interacts with RIPOR2 (via phosphorylated form); this interaction occurs in a chemokine-dependent manner and does not compete for binding of RIPOR2 with RHOA nor blocks inhibition of RIPOR2-mediated RHOA activity (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	58	Interaction with phosphoserine on interacting proteinBy similarity		1
Siteⁱ	129	Interaction with phosphoserine on interacting proteinBy similarity		1

GO - Molecular functionⁱ

protein domain specific binding Source: AgBase

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	9913.ENSBTAP00000022411

STRINGⁱ

9913.ENSBTAP00000022411

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P68250
SMRⁱ	P68250
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0841 Eukaryota COG5040 LUCA
HOGENOMⁱ	HOG000240379
HOVERGENⁱ	HBG050423
InParanoidⁱ	P68250
KEGG Orthology (KO) More...KOⁱ	K16197
TreeFamⁱ	TF102003

Family and domain databases

Gene3Dⁱ	1.20.190.20, 1 hit
InterProⁱ	View protein in InterPro IPR000308 14-3-3 IPR023409 14-3-3_CS IPR036815 14-3-3_dom_sf IPR023410 14-3-3_domain
PANTHERⁱ	PTHR18860 PTHR18860, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00244 14-3-3, 1 hit
PIRSFⁱ	PIRSF000868 14-3-3, 1 hit
PRINTSⁱ	PR00305 1433ZETA
SMARTⁱ	View protein in SMART SM00101 14_3_3, 1 hit
SUPFAMⁱ	SSF48445 SSF48445, 1 hit
PROSITEⁱ	View protein in PROSITE PS00796 1433_1, 1 hit PS00797 1433_2, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative initiation. Align Add to basket Added to basket

Note: It is uncertain whether isoform Short is produced by alternative initiation or another biological event.

Isoform Long (identifier: P68250-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY 
        60         70         80         90        100
KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL 
       110        120        130        140        150
QLLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA 
       160        170        180        190        200
YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE 
       210        220        230        240 
AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN

Length:246

Mass (Da):28,081

Last modified:January 23, 2007 - v2

Checksum:ⁱCABA32314D86800D

GO

Isoform Short (identifier: P68250-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-2: Missing.

« Hide

        10         20         30         40         50
MDKSELVQKA KLAEQAERYD DMAAAMKAVT EQGHELSNEE RNLLSVAYKN 
        60         70         80         90        100
VVGARRSSWR VISSIEQKTE RNEKKQQMGK EYREKIEAEL QDICNDVLQL 
       110        120        130        140        150
LDKYLIPNAT QPESKVFYLK MKGDYFRYLS EVASGDNKQT TVSNSQQAYQ 
       160        170        180        190        200
EAFEISKKEM QPTHPIRLGL ALNFSVFYYE ILNSPEKACS LAKTAFDEAI 
       210        220        230        240 
AELDTLNEES YKDSTLIMQL LRDNLTLWTS ENQGDEGDAG EGEN

Show »

Length:244

Mass (Da):27,849

Checksum:ⁱ6F51C23156F3FE35

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	101	Q → E in AAI20113 (Ref. 1) Curated		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_018631	1 – 2	Missing in isoform Short. 1 Publication		2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC120112 mRNA Translation: AAI20113.1 AF043736 mRNA Translation: AAC02090.1
PIRⁱ	S13467
NCBI Reference Sequences More...RefSeqⁱ	NP_777219.2, NM_174794.2
UniGeneⁱ	Bt.5535

Genome annotation databases

GeneIDⁱ	286863
KEGGⁱ	bta:286863

Keywords - Coding sequence diversityⁱ

Alternative initiation

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
P68250	14-3-3 protein beta/alpha		RAT		246
14-3-3 protein beta/alpha		MYOBR		304
UPI000D721A30		ALLSI		279
14-3-3 protein beta/alpha		TUPCH		270
UPI000A32B1E9		HUMAN		249
+97

Protein	Similar proteins		Species	Score	Length
P68250	14-3-3 protein theta	)	HUMAN		245
14-3-3 protein theta		BOVIN		245
14-3-3 protein theta		PONAB		245
14-3-3 protein theta		CALJA		245
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide		PANTR		245
+311

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BC120112 mRNA Translation: AAI20113.1 AF043736 mRNA Translation: AAC02090.1
PIRⁱ	S13467
NCBI Reference Sequences More...RefSeqⁱ	NP_777219.2, NM_174794.2
UniGeneⁱ	Bt.5535

3D structure databases

ProteinModelPortalⁱ	P68250
SMRⁱ	P68250
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	9913.ENSBTAP00000022411

STRINGⁱ

9913.ENSBTAP00000022411

PTM databases

iPTMnetⁱ	P68250

iPTMnetⁱ

P68250

Proteomic databases

PaxDbⁱ	P68250
PeptideAtlas More...PeptideAtlasⁱ	P68250
PRIDEⁱ	P68250

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	286863
KEGGⁱ	bta:286863

Organism-specific databases

CTDⁱ	7529

CTDⁱ

7529

Phylogenomic databases

eggNOGⁱ	KOG0841 Eukaryota COG5040 LUCA
HOGENOMⁱ	HOG000240379
HOVERGENⁱ	HBG050423
InParanoidⁱ	P68250
KEGG Orthology (KO) More...KOⁱ	K16197
TreeFamⁱ	TF102003

Family and domain databases

Gene3Dⁱ	1.20.190.20, 1 hit
InterProⁱ	View protein in InterPro IPR000308 14-3-3 IPR023409 14-3-3_CS IPR036815 14-3-3_dom_sf IPR023410 14-3-3_domain
PANTHERⁱ	PTHR18860 PTHR18860, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00244 14-3-3, 1 hit
PIRSFⁱ	PIRSF000868 14-3-3, 1 hit
PRINTSⁱ	PR00305 1433ZETA
SMARTⁱ	View protein in SMART SM00101 14_3_3, 1 hit
SUPFAMⁱ	SSF48445 SSF48445, 1 hit
PROSITEⁱ	View protein in PROSITE PS00796 1433_1, 1 hit PS00797 1433_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	1433B_BOVIN
Accessionⁱ	P68250Primary (citable) accession number: P68250 Secondary accession number(s): P29358, Q0VCL1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
	Last sequence update:	January 23, 2007
	Last modified:	October 25, 2017
	This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - P68250 (1433B_BOVIN)

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14-3-3 protein beta/alpha

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Alternative sequence

Sequence databases

Genome annotation databases

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Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ