UniProtKB - P68250 (1433B_BOVIN)
14-3-3 protein beta/alpha
YWHAB
Functioni
GO - Molecular functioni
- protein domain specific binding Source: AgBase
- protein kinase inhibitor activity Source: UniProtKB
GO - Biological processi
- negative regulation of G protein-coupled receptor signaling pathway Source: UniProtKB
- protein targeting Source: AgBase
Names & Taxonomyi
Protein namesi | Recommended name: 14-3-3 protein beta/alphaAlternative name(s): Protein kinase C inhibitor protein 1 Short name: KCIP-1 Cleaved into the following chain: |
Gene namesi | Name:YWHAB |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Subcellular locationi
Other locations
- Cytoplasm By similarity
- Melanosome By similarity
Other locations
- cytoplasm Source: AgBase
- melanosome Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: AgBase
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000367899 | 1 – 246 | 14-3-3 protein beta/alphaAdd BLAST | 246 | |
Initiator methioninei | Removed; alternateBy similarity1 Publication | |||
ChainiPRO_0000000001 | 2 – 246 | 14-3-3 protein beta/alpha, N-terminally processedAdd BLAST | 245 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | ||
Modified residuei | 2 | N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processedBy similarity | 1 | ||
Modified residuei | 2 | PhosphothreonineBy similarity | 1 | ||
Modified residuei | 5 | N6-acetyllysineBy similarity | 1 | ||
Modified residuei | 51 | N6-acetyllysine; alternateBy similarity | 1 | ||
Cross-linki | 51 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | |||
Modified residuei | 60 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 70 | N6-acetyllysineBy similarity | 1 | ||
Modified residuei | 84 | 3'-nitrotyrosineBy similarity | 1 | ||
Modified residuei | 106 | 3'-nitrotyrosineBy similarity | 1 | ||
Modified residuei | 117 | N6-acetyllysineBy similarity | 1 | ||
Modified residuei | 186 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 232 | PhosphoserineBy similarity | 1 | ||
Isoform Short (identifier: P68250-2) | |||||
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Nitration, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P68250 |
PeptideAtlasi | P68250 |
PRIDEi | P68250 |
PTM databases
iPTMneti | P68250 |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with SAMSN1 and PRKCE (By similarity).
Interacts with AKAP13.
Interacts with SSH1 and TORC2/CRTC2.
Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis.
Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues.
Interacts with GAB2.
Interacts with YAP1 (phosphorylated form).
Interacts with the phosphorylated (by AKT1) form of SRPK2.
Interacts with PKA-phosphorylated AANAT.
Interacts with MYO1C.
Interacts with SIRT2 (By similarity).
Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity).
Interacts with PI4KB, TBC1D22A and TBC1D22B.
Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity).
Interacts (via phosphorylated form) with YWHAB; this interaction occurs in a protein kinase AKT1-dependent manner (By similarity).
Interacts with SLITRK1.
Interacts with SYNPO2 (phosphorylated form); YWHAB competes with ACTN2 for interaction with SYNPO2 (By similarity).
Interacts with RIPOR2 (via phosphorylated form); this interaction occurs in a chemokine-dependent manner and does not compete for binding of RIPOR2 with RHOA nor blocks inhibition of RIPOR2-mediated RHOA activity (By similarity).
Interacts with MARK2 and MARK3 (By similarity).
Interacts with TESK1; the interaction is dependent on the phosphorylation of TESK1 'Ser-439' and inhibits TESK1 kinase activity (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 58 | Interaction with phosphoserine on interacting proteinBy similarity | 1 | |
Sitei | 129 | Interaction with phosphoserine on interacting proteinBy similarity | 1 |
GO - Molecular functioni
- protein domain specific binding Source: AgBase
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000022411 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0841, Eukaryota |
HOGENOMi | CLU_058290_1_0_1 |
InParanoidi | P68250 |
OrthoDBi | 1176818at2759 |
TreeFami | TF102003 |
Family and domain databases
Gene3Di | 1.20.190.20, 1 hit |
InterProi | View protein in InterPro IPR000308, 14-3-3 IPR023409, 14-3-3_CS IPR036815, 14-3-3_dom_sf IPR023410, 14-3-3_domain |
PANTHERi | PTHR18860, PTHR18860, 1 hit |
Pfami | View protein in Pfam PF00244, 14-3-3, 1 hit |
PIRSFi | PIRSF000868, 14-3-3, 1 hit |
PRINTSi | PR00305, 1433ZETA |
SMARTi | View protein in SMART SM00101, 14_3_3, 1 hit |
SUPFAMi | SSF48445, SSF48445, 1 hit |
PROSITEi | View protein in PROSITE PS00796, 1433_1, 1 hit PS00797, 1433_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY
60 70 80 90 100
KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL
110 120 130 140 150
QLLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA
160 170 180 190 200
YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE
210 220 230 240
AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
The sequence of this isoform differs from the canonical sequence as follows:
1-2: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 101 | Q → E in AAI20113 (Ref. 1) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018631 | 1 – 2 | Missing in isoform Short. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC120112 mRNA Translation: AAI20113.1 AF043736 mRNA Translation: AAC02090.1 |
PIRi | S13467 |
RefSeqi | NP_777219.2, NM_174794.2 |
Genome annotation databases
GeneIDi | 286863 |
KEGGi | bta:286863 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC120112 mRNA Translation: AAI20113.1 AF043736 mRNA Translation: AAC02090.1 |
PIRi | S13467 |
RefSeqi | NP_777219.2, NM_174794.2 |
3D structure databases
SMRi | P68250 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000022411 |
PTM databases
iPTMneti | P68250 |
Proteomic databases
PaxDbi | P68250 |
PeptideAtlasi | P68250 |
PRIDEi | P68250 |
Genome annotation databases
GeneIDi | 286863 |
KEGGi | bta:286863 |
Organism-specific databases
CTDi | 7529 |
Phylogenomic databases
eggNOGi | KOG0841, Eukaryota |
HOGENOMi | CLU_058290_1_0_1 |
InParanoidi | P68250 |
OrthoDBi | 1176818at2759 |
TreeFami | TF102003 |
Family and domain databases
Gene3Di | 1.20.190.20, 1 hit |
InterProi | View protein in InterPro IPR000308, 14-3-3 IPR023409, 14-3-3_CS IPR036815, 14-3-3_dom_sf IPR023410, 14-3-3_domain |
PANTHERi | PTHR18860, PTHR18860, 1 hit |
Pfami | View protein in Pfam PF00244, 14-3-3, 1 hit |
PIRSFi | PIRSF000868, 14-3-3, 1 hit |
PRINTSi | PR00305, 1433ZETA |
SMARTi | View protein in SMART SM00101, 14_3_3, 1 hit |
SUPFAMi | SSF48445, SSF48445, 1 hit |
PROSITEi | View protein in PROSITE PS00796, 1433_1, 1 hit PS00797, 1433_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 1433B_BOVIN | |
Accessioni | P68250Primary (citable) accession number: P68250 Secondary accession number(s): P29358, Q0VCL1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 118 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families