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Protein

Interleukin-12 subunit beta

Gene

IL12B

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.By similarity
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis (By similarity).By similarity

GO - Molecular functioni

  • cytokine activity Source: UniProtKB-KW
  • cytokine receptor activity Source: InterPro
  • interleukin-12 alpha subunit binding Source: AgBase
  • protein heterodimerization activity Source: AgBase

GO - Biological processi

  • interferon-gamma secretion Source: AgBase
  • positive regulation of mononuclear cell proliferation Source: AgBase

Keywordsi

Molecular functionCytokine

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 subunit beta
Short name:
IL-12B
Alternative name(s):
Cytotoxic lymphocyte maturation factor 40 kDa subunit
Short name:
CLMF p40
IL-12 subunit p40
Gene namesi
Name:IL12B
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001093623 – 327Interleukin-12 subunit betaAdd BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 90PROSITE-ProRule annotation
Disulfide bondi200Interchain (with C-98 in IL12A)PROSITE-ProRule annotation
Glycosylationi223N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Heterodimer with IL12A; disulfide-linked. The heterodimer is known as interleukin IL-12. Heterodimer with IL23A; disulfide-linked. The heterodimer is known as interleukin IL-23. Also secreted as a monomer (By similarity).By similarity

GO - Molecular functioni

  • cytokine activity Source: UniProtKB-KW
  • interleukin-12 alpha subunit binding Source: AgBase
  • protein heterodimerization activity Source: AgBase

Structurei

3D structure databases

ProteinModelPortaliP68220
SMRiP68220
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 106Ig-like C2-typeAdd BLAST84
Domaini238 – 327Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST90

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

HOVERGENiHBG052094
KOiK05425

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 3 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR003530 Hematopoietin_rcpt_L_F3_CS
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003598 Ig_sub2
IPR015528 IL-12_beta
IPR019482 IL-12_beta_cen-dom
PANTHERiPTHR44982 PTHR44982, 1 hit
PfamiView protein in Pfam
PF10420 IL12p40_C, 1 hit
PIRSFiPIRSF038007 IL_12_beta, 1 hit
PRINTSiPR01928 INTRLEUKN12B
SMARTiView protein in SMART
SM00408 IGc2, 1 hit
SUPFAMiSSF48726 SSF48726, 1 hit
SSF49265 SSF49265, 2 hits
PROSITEiView protein in PROSITE
PS50853 FN3, 1 hit
PS01354 HEMATOPO_REC_L_F3, 1 hit
PS50835 IG_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPQQLVVSW FSLVLLASPI VAIWELEKNV YVVELDWYPN APGETVVLTC
60 70 80 90 100
DTPEEDGITW TSDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEVLSRS
110 120 130 140 150
LLLLHKKEDG IWSTDILKDQ KEPKAKSFLK CEAKDYSGHF TCSWLTAIST
160 170 180 190 200
NLKFSVKSSR GSSDPRGVTC GAASLSAEKV SMDHREYNKY TVECQEGSAC
210 220 230 240 250
PAAEESLPIE VVMEAVHKLK YENYTSSFFI RDIIKPDPPK NLQLRPLKNS
260 270 280 290 300
RQVEVSWEYP DTWSTPHSYF SLTFCVQVQG KNKREKKLFT DQTSAKVTCH
310 320
KDANIRVQAR DRYYSSFWSE WASVSCS
Length:327
Mass (Da):36,937
Last modified:October 25, 2004 - v1
Checksum:iDDCD983A10143A96
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti290T → A in AAF20151 (PubMed:10888112).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004024 mRNA Translation: AAB61368.2
AF209435 mRNA Translation: AAF20151.1
RefSeqiNP_001009438.1, NM_001009438.1
UniGeneiOar.721

Genome annotation databases

GeneIDi443472
KEGGioas:443472

Similar proteinsi

Entry informationi

Entry nameiIL12B_SHEEP
AccessioniPrimary (citable) accession number: P68220
Secondary accession number(s): O02815, O18989, Q9TT18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: December 20, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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