Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Strictosidine synthase

Gene

STR1

Organism
Rauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis.

Catalytic activityi

3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

Pathwayi: 3alpha(S)-strictosidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 3alpha(S)-strictosidine from secologanin and tryptamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Strictosidine synthase (STR1)
This subpathway is part of the pathway 3alpha(S)-strictosidine biosynthesis, which is itself part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3alpha(S)-strictosidine from secologanin and tryptamine, the pathway 3alpha(S)-strictosidine biosynthesis and in Alkaloid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAlkaloid metabolism

Enzyme and pathway databases

BRENDAi4.3.3.2 5309
UniPathwayi
UPA00311;UER00447

Names & Taxonomyi

Protein namesi
Recommended name:
Strictosidine synthase (EC:4.3.3.2)
Gene namesi
Name:STR1
OrganismiRauvolfia serpentina (Serpentine wood) (Ophioxylon serpentinum)
Taxonomic identifieri4060 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesApocynaceaeRauvolfioideaeVinceaeRauvolfiinaeRauvolfia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000003333423 – 344Strictosidine synthaseAdd BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi91N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed predominantly, but not exclusively, in the root.1 Publication

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1344
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP68175
SMRiP68175
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68175

Family & Domainsi

Sequence similaritiesi

Belongs to the strictosidine synthase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK01757

Family and domain databases

Gene3Di2.120.10.30, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR018119 Strictosidine_synth_cons-reg
IPR004141 Strictosidine_synthase
PANTHERiPTHR10426 PTHR10426, 1 hit
PfamiView protein in Pfam
PF03088 Str_synth, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68175-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKLSDSQTM ALFTVFLLFL SSSLALSSPI LKEILIEAPS YAPNSFTFDS
60 70 80 90 100
TNKGFYTSVQ DGRVIKYEGP NSGFVDFAYA SPYWNKAFCE NSTDAEKRPL
110 120 130 140 150
CGRTYDISYN LQNNQLYIVD CYYHLSVVGS EGGHATQLAT SVDGVPFKWL
160 170 180 190 200
YAVTVDQRTG IVYFTDVSTL YDDRGVQQIM DTSDKTGRLI KYDPSTKETT
210 220 230 240 250
LLLKELHVPG GAEVSADSSF VLVAEFLSHQ IVKYWLEGPK KGTAEVLVKI
260 270 280 290 300
PNPGNIKRNA DGHFWVSSSE ELDGNMHGRV DPKGIKFDEF GNILEVIPLP
310 320 330 340
PPFAGEHFEQ IQEHDGLLYI GTLFHGSVGI LVYDKKGNSF VSSH
Length:344
Mass (Da):38,162
Last modified:October 25, 2004 - v1
Checksum:i7CD38882621F768A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00756 mRNA Translation: CAA68725.1
X62334 Genomic DNA Translation: CAA44208.1
PIRiS01325

Genome annotation databases

KEGGiag:CAA44208

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00756 mRNA Translation: CAA68725.1
X62334 Genomic DNA Translation: CAA44208.1
PIRiS01325

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FP8X-ray2.30A/B23-344[»]
2FP9X-ray2.96A/B23-344[»]
2FPBX-ray2.80A/B23-344[»]
2FPCX-ray3.00A/B23-344[»]
2V91X-ray3.01A/B32-333[»]
2VAQX-ray3.01A/B23-344[»]
3V1SX-ray2.33A/B23-344[»]
4IMBX-ray2.70A/B32-332[»]
4IYGX-ray2.70A/B32-333[»]
ProteinModelPortaliP68175
SMRiP68175
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA44208

Phylogenomic databases

KOiK01757

Enzyme and pathway databases

UniPathwayi
UPA00311;UER00447

BRENDAi4.3.3.2 5309

Miscellaneous databases

EvolutionaryTraceiP68175

Family and domain databases

Gene3Di2.120.10.30, 1 hit
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR018119 Strictosidine_synth_cons-reg
IPR004141 Strictosidine_synthase
PANTHERiPTHR10426 PTHR10426, 1 hit
PfamiView protein in Pfam
PF03088 Str_synth, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTSY_RAUSE
AccessioniPrimary (citable) accession number: P68175
Secondary accession number(s): P15324
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: October 25, 2004
Last modified: February 28, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again