UniProtKB - P68104 (EF1A1_HUMAN)
Elongation factor 1-alpha 1
EEF1A1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 14 – 21 | GTPBy similarity | 8 | |
Nucleotide bindingi | 91 – 95 | GTPBy similarity | 5 | |
Nucleotide bindingi | 153 – 156 | GTPBy similarity | 4 |
GO - Molecular functioni
- calmodulin binding Source: Ensembl
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB
- kinase binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
- RNA binding Source: UniProtKB
- translation elongation factor activity Source: GO_Central
- tRNA binding Source: UniProtKB
GO - Biological processi
- cellular response to epidermal growth factor stimulus Source: UniProtKB
- neutrophil degranulation Source: Reactome
- regulation of chaperone-mediated autophagy Source: ParkinsonsUK-UCL
- regulation of D-erythro-sphingosine kinase activity Source: UniProtKB
- translation Source: GO_Central
- translational elongation Source: GO_Central
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis, Transcription, Transcription regulation |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P68104 |
Reactomei | R-HSA-156842, Eukaryotic Translation Elongation R-HSA-156902, Peptide chain elongation R-HSA-3371511, HSF1 activation R-HSA-6798695, Neutrophil degranulation R-HSA-8876725, Protein methylation R-HSA-9613829, Chaperone Mediated Autophagy |
SignaLinki | P68104 |
SIGNORi | P68104 |
Protein family/group databases
MoonProti | P68104 |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor 1-alpha 1Short name: EF-1-alpha-1 Alternative name(s): Elongation factor Tu Short name: EF-Tu Eukaryotic elongation factor 1 A-1 Short name: eEF1A-1 Leukocyte receptor cluster member 7 |
Gene namesi | Name:EEF1A1 Synonyms:EEF1A, EF1A, LENG7 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3189, EEF1A1 |
MIMi | 130590, gene |
neXtProti | NX_P68104 |
VEuPathDBi | HostDB:ENSG00000156508.17 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Nucleus
Other locations
- Cytoplasm 2 Publications
Note: Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934).3 Publications
Cytoskeleton
- cortical actin cytoskeleton Source: UniProtKB
Cytosol
- cytosol Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
Lysosome
- cytoplasmic side of lysosomal membrane Source: ParkinsonsUK-UCL
Nucleus
Plasma Membrane
- plasma membrane Source: UniProtKB
- ruffle membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- eukaryotic translation elongation factor 1 complex Source: UniProtKB
- ficolin-1-rich granule lumen Source: Reactome
- membrane Source: UniProtKB
- secretory granule lumen Source: Reactome
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 36 | K → R: No effect on methylation by EEF1AKMT2. Abolishes EEF1AKMT4-mediated methylation. 2 Publications | 1 | |
Mutagenesisi | 55 | K → R: No effect on methylation by EEF1AKMT2. Abolishes methylation by EEF1AKNMT. 3 Publications | 1 | |
Mutagenesisi | 79 | K → R: No effect on methylation by EEF1AKMT2. 1 Publication | 1 | |
Mutagenesisi | 165 | K → A: Abolishes methylation by EEF1AKMT3. 1 Publication | 1 | |
Mutagenesisi | 165 | K → R: No effect on methylation by EEF1AKMT2. 1 Publication | 1 | |
Mutagenesisi | 318 | K → R: Abolishes methylation by EEF1AKMT2. 1 Publication | 1 | |
Mutagenesisi | 432 | T → A: Abolishes phosphorylation by PASK. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 1915 |
OpenTargetsi | ENSG00000156508 |
PharmGKBi | PA27625 |
Miscellaneous databases
Pharosi | P68104, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1795120 |
DrugBanki | DB11638, Artenimol DB09130, Copper DB04315, Guanosine-5'-Diphosphate DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
Genetic variation databases
BioMutai | EEF1A1 |
DMDMi | 55584035 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000090885 | 2 – 462 | Elongation factor 1-alpha 1Add BLAST | 461 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N,N,N-trimethylglycine2 Publications | 1 | |
Modified residuei | 36 | N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT41 Publication | 1 | |
Modified residuei | 36 | N6,N6-dimethyllysine; alternate; by EEF1AKMT41 Publication | 1 | |
Modified residuei | 36 | N6-methyllysine; alternate; by EEF1AKMT41 Publication | 1 | |
Modified residuei | 55 | N6,N6-dimethyllysine3 Publications | 1 | |
Modified residuei | 79 | N6,N6,N6-trimethyllysine; by EEF1AKMT11 Publication | 1 | |
Modified residuei | 165 | N6,N6,N6-trimethyllysine; alternate; by EEF1AKMT31 Publication | 1 | |
Modified residuei | 165 | N6,N6-dimethyllysine; alternate; by EEF1AKMT32 Publications | 1 | |
Modified residuei | 165 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 165 | N6-methyllysine; alternate; by EEF1AKMT31 Publication | 1 | |
Modified residuei | 172 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 273 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 300 | Phosphoserine; by TGFBR11 Publication | 1 | |
Modified residuei | 301 | 5-glutamyl glycerylphosphorylethanolamine1 Publication | 1 | |
Modified residuei | 318 | N6,N6,N6-trimethyllysine; by EEF1AKMT21 Publication | 1 | |
Modified residuei | 374 | 5-glutamyl glycerylphosphorylethanolamine1 Publication | 1 | |
Modified residuei | 392 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 392 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 432 | Phosphothreonine; by PASK1 Publication | 1 | |
Modified residuei | 439 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P68104 |
jPOSTi | P68104 |
MassIVEi | P68104 |
PaxDbi | P68104 |
PeptideAtlasi | P68104 |
PRIDEi | P68104 |
ProteomicsDBi | 66470 |
2D gel databases
OGPi | P68104 |
SWISS-2DPAGEi | P68104 |
UCD-2DPAGEi | P68104 |
PTM databases
GlyGeni | P68104, 1 site |
iPTMneti | P68104 |
MetOSitei | P68104 |
PhosphoSitePlusi | P68104 |
SwissPalmi | P68104 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000156508, Expressed in subventricular zone (outer) (primate) and 228 other tissues |
ExpressionAtlasi | P68104, baseline and differential |
Genevisiblei | P68104, HS |
Organism-specific databases
HPAi | ENSG00000156508, Low tissue specificity |
Interactioni
Subunit structurei
Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA (PubMed:12426392, PubMed:12426393).
Interacts with PARP1 and TXK (PubMed:17177976).
Interacts with KARS1 (PubMed:18029264). May interact with ERGIC2.
Interacts with IFIT1 (via TPR repeats 4-7) (By similarity).
Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation.
Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner.
Interacts with PPP1R16B (PubMed:26497934).
Interacts with SPHK1 and SPHK2; both interactions increase SPHK1 and SPHK2 kinase activity (PubMed:18263879).
By similarity9 PublicationsBinary interactionsi
Hide detailsP68104
GO - Molecular functioni
- calmodulin binding Source: Ensembl
- kinase binding Source: UniProtKB
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 108237, 497 interactors |
CORUMi | P68104 |
DIPi | DIP-31277N |
IntActi | P68104, 236 interactors |
MINTi | P68104 |
STRINGi | 9606.ENSP00000339063 |
Chemistry databases
BindingDBi | P68104 |
Miscellaneous databases
RNActi | P68104, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 242 | tr-type GAdd BLAST | 238 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 14 – 21 | G1By similarity | 8 | |
Regioni | 70 – 74 | G2By similarity | 5 | |
Regioni | 91 – 94 | G3By similarity | 4 | |
Regioni | 153 – 156 | G4By similarity | 4 | |
Regioni | 194 – 196 | G5By similarity | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0052, Eukaryota |
GeneTreei | ENSGT00950000183029 |
InParanoidi | P68104 |
OMAi | ICAINKM |
PhylomeDBi | P68104 |
TreeFami | TF300304 |
Family and domain databases
HAMAPi | MF_00118_A, EF_Tu_A, 1 hit |
InterProi | View protein in InterPro IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004539, Transl_elong_EF1A_euk/arc IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00483, EF-1_alpha, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGKEKTHINI VVIGHVDSGK STTTGHLIYK CGGIDKRTIE KFEKEAAEMG
60 70 80 90 100
KGSFKYAWVL DKLKAERERG ITIDISLWKF ETSKYYVTII DAPGHRDFIK
110 120 130 140 150
NMITGTSQAD CAVLIVAAGV GEFEAGISKN GQTREHALLA YTLGVKQLIV
160 170 180 190 200
GVNKMDSTEP PYSQKRYEEI VKEVSTYIKK IGYNPDTVAF VPISGWNGDN
210 220 230 240 250
MLEPSANMPW FKGWKVTRKD GNASGTTLLE ALDCILPPTR PTDKPLRLPL
260 270 280 290 300
QDVYKIGGIG TVPVGRVETG VLKPGMVVTF APVNVTTEVK SVEMHHEALS
310 320 330 340 350
EALPGDNVGF NVKNVSVKDV RRGNVAGDSK NDPPMEAAGF TAQVIILNHP
360 370 380 390 400
GQISAGYAPV LDCHTAHIAC KFAELKEKID RRSGKKLEDG PKFLKSGDAA
410 420 430 440 450
IVDMVPGKPM CVESFSDYPP LGRFAVRDMR QTVAVGVIKA VDKKAAGAGK
460
VTKSAQKAQK AK
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WV01 | A0A087WV01_HUMAN | Elongation factor 1-alpha | EEF1A1 | 426 | Annotation score: | ||
A0A087WVQ9 | A0A087WVQ9_HUMAN | Elongation factor 1-alpha 1 | EEF1A1 | 441 | Annotation score: | ||
A6PW80 | A6PW80_HUMAN | Elongation factor 1-alpha 1 | EEF1A1 | 106 | Annotation score: | ||
Q5JR01 | Q5JR01_HUMAN | Elongation factor 1-alpha 1 | EEF1A1 | 145 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 83 | S → A in CAA27325 (PubMed:3960725).Curated | 1 | |
Sequence conflicti | 232 | L → V in CAA34756 (PubMed:2183196).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03558 mRNA Translation: CAA27245.1 J04617 Genomic DNA Translation: AAA52343.1 X16869 mRNA Translation: CAA34756.1 AY043301 mRNA Translation: AAK95378.1 BC008587 mRNA Translation: AAH08587.1 BC009733 mRNA Translation: AAH09733.1 BC009875 mRNA Translation: AAH09875.1 BC010735 mRNA Translation: AAH10735.1 BC012891 mRNA Translation: AAH12891.1 BC014224 mRNA Translation: AAH14224.1 BC018150 mRNA Translation: AAH18150.1 BC018641 mRNA Translation: AAH18641.1 BC021686 mRNA Translation: AAH21686.1 BC028674 mRNA Translation: AAH28674.1 BC038339 mRNA Translation: AAH38339.1 BC057391 mRNA Translation: AAH57391.1 BC066893 mRNA Translation: AAH66893.1 BC071619 mRNA Translation: AAH71619.1 Sequence problems. BC072385 mRNA Translation: AAH72385.1 BC082268 mRNA Translation: AAH82268.1 X03689 mRNA Translation: CAA27325.1 M29548 mRNA Translation: AAA52367.1 Different initiation. |
CCDSi | CCDS4980.1 |
PIRi | B24977, EFHU1 |
RefSeqi | NP_001393.1, NM_001402.5 XP_011533816.1, XM_011535514.2 |
Genome annotation databases
Ensembli | ENST00000309268; ENSP00000339053; ENSG00000156508 ENST00000316292; ENSP00000339063; ENSG00000156508 ENST00000331523; ENSP00000330054; ENSG00000156508 |
GeneIDi | 1915 |
Similar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X03558 mRNA Translation: CAA27245.1 J04617 Genomic DNA Translation: AAA52343.1 X16869 mRNA Translation: CAA34756.1 AY043301 mRNA Translation: AAK95378.1 BC008587 mRNA Translation: AAH08587.1 BC009733 mRNA Translation: AAH09733.1 BC009875 mRNA Translation: AAH09875.1 BC010735 mRNA Translation: AAH10735.1 BC012891 mRNA Translation: AAH12891.1 BC014224 mRNA Translation: AAH14224.1 BC018150 mRNA Translation: AAH18150.1 BC018641 mRNA Translation: AAH18641.1 BC021686 mRNA Translation: AAH21686.1 BC028674 mRNA Translation: AAH28674.1 BC038339 mRNA Translation: AAH38339.1 BC057391 mRNA Translation: AAH57391.1 BC066893 mRNA Translation: AAH66893.1 BC071619 mRNA Translation: AAH71619.1 Sequence problems. BC072385 mRNA Translation: AAH72385.1 BC082268 mRNA Translation: AAH82268.1 X03689 mRNA Translation: CAA27325.1 M29548 mRNA Translation: AAA52367.1 Different initiation. |
CCDSi | CCDS4980.1 |
PIRi | B24977, EFHU1 |
RefSeqi | NP_001393.1, NM_001402.5 XP_011533816.1, XM_011535514.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1SYW | model | - | A | 2-443 | [»] | |
3C5J | X-ray | 1.80 | C | 343-355 | [»] | |
6ZMO | electron microscopy | 3.10 | CD | 1-462 | [»] | |
SMRi | P68104 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 108237, 497 interactors |
CORUMi | P68104 |
DIPi | DIP-31277N |
IntActi | P68104, 236 interactors |
MINTi | P68104 |
STRINGi | 9606.ENSP00000339063 |
Chemistry databases
BindingDBi | P68104 |
ChEMBLi | CHEMBL1795120 |
DrugBanki | DB11638, Artenimol DB09130, Copper DB04315, Guanosine-5'-Diphosphate DB01593, Zinc DB14487, Zinc acetate DB14533, Zinc chloride DB14548, Zinc sulfate, unspecified form |
Protein family/group databases
MoonProti | P68104 |
PTM databases
GlyGeni | P68104, 1 site |
iPTMneti | P68104 |
MetOSitei | P68104 |
PhosphoSitePlusi | P68104 |
SwissPalmi | P68104 |
Genetic variation databases
BioMutai | EEF1A1 |
DMDMi | 55584035 |
2D gel databases
OGPi | P68104 |
SWISS-2DPAGEi | P68104 |
UCD-2DPAGEi | P68104 |
Proteomic databases
EPDi | P68104 |
jPOSTi | P68104 |
MassIVEi | P68104 |
PaxDbi | P68104 |
PeptideAtlasi | P68104 |
PRIDEi | P68104 |
ProteomicsDBi | 66470 |
Protocols and materials databases
Antibodypediai | 31353, 317 antibodies |
DNASUi | 1915 |
Genome annotation databases
Ensembli | ENST00000309268; ENSP00000339053; ENSG00000156508 ENST00000316292; ENSP00000339063; ENSG00000156508 ENST00000331523; ENSP00000330054; ENSG00000156508 |
GeneIDi | 1915 |
Organism-specific databases
CTDi | 1915 |
DisGeNETi | 1915 |
GeneCardsi | EEF1A1 |
HGNCi | HGNC:3189, EEF1A1 |
HPAi | ENSG00000156508, Low tissue specificity |
MIMi | 130590, gene |
neXtProti | NX_P68104 |
OpenTargetsi | ENSG00000156508 |
PharmGKBi | PA27625 |
VEuPathDBi | HostDB:ENSG00000156508.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0052, Eukaryota |
GeneTreei | ENSGT00950000183029 |
InParanoidi | P68104 |
OMAi | ICAINKM |
PhylomeDBi | P68104 |
TreeFami | TF300304 |
Enzyme and pathway databases
PathwayCommonsi | P68104 |
Reactomei | R-HSA-156842, Eukaryotic Translation Elongation R-HSA-156902, Peptide chain elongation R-HSA-3371511, HSF1 activation R-HSA-6798695, Neutrophil degranulation R-HSA-8876725, Protein methylation R-HSA-9613829, Chaperone Mediated Autophagy |
SignaLinki | P68104 |
SIGNORi | P68104 |
Miscellaneous databases
BioGRID-ORCSi | 1915, 695 hits in 865 CRISPR screens |
ChiTaRSi | EEF1A1, human |
GeneWikii | Eukaryotic_translation_elongation_factor_1_alpha_1 |
GenomeRNAii | 1915 |
Pharosi | P68104, Tchem |
PROi | PR:P68104 |
RNActi | P68104, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000156508, Expressed in subventricular zone (outer) (primate) and 228 other tissues |
ExpressionAtlasi | P68104, baseline and differential |
Genevisiblei | P68104, HS |
Family and domain databases
HAMAPi | MF_00118_A, EF_Tu_A, 1 hit |
InterProi | View protein in InterPro IPR004161, EFTu-like_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004539, Transl_elong_EF1A_euk/arc IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00483, EF-1_alpha, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EF1A1_HUMAN | |
Accessioni | P68104Primary (citable) accession number: P68104 Secondary accession number(s): P04719, P04720, Q6IQ15 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 1987 |
Last sequence update: | August 13, 1987 | |
Last modified: | February 10, 2021 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families