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UniProtKB - P68066 (GRCA_ECOLI)
Protein
Autonomous glycyl radical cofactor
Gene
grcA
Organism
Escherichia coli (strain K12)
Status
Functioni
Acts as a radical domain for damaged PFL and possibly other radical proteins.
1 PublicationGO - Molecular functioni
- formate C-acetyltransferase activity Source: GO_Central
GO - Biological processi
- threonine catabolic process Source: GO_Central
Keywordsi
Biological process | Stress response |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11784-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Autonomous glycyl radical cofactor |
Gene namesi | Name:grcA Synonyms:yfiD Ordered Locus Names:b2579, JW2563 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Disruption phenotypei
Cells undergo an apoptotic-like death upon DNA damage characterized by membrane depolarization; further disruption of recA prevents membrane depolarization.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000166697 | 1 – 127 | Autonomous glycyl radical cofactorAdd BLAST | 127 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 88 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 92 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 102 | Glycine radicalBy similarity | 1 |
Keywords - PTMi
Acetylation, Organic radicalProteomic databases
jPOSTi | P68066 |
PaxDbi | P68066 |
PRIDEi | P68066 |
2D gel databases
SWISS-2DPAGEi | P68066 |
PTM databases
iPTMneti | P68066 |
Expressioni
Inductioni
By acid stress.1 Publication
Interactioni
Binary interactionsi
P68066
With | #Exp. | IntAct |
---|---|---|
slyD [P0A9K9] | 3 | EBI-561424,EBI-369251 |
Protein-protein interaction databases
BioGRIDi | 4263475, 8 interactors |
DIPi | DIP-47913N |
IntActi | P68066, 18 interactors |
STRINGi | 511145.b2579 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P68066 |
SMRi | P68066 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 127 | Glycine radicalAdd BLAST | 123 |
Phylogenomic databases
eggNOGi | COG3445, Bacteria |
HOGENOMi | CLU_133780_0_0_6 |
OMAi | LGQFEYR |
PhylomeDBi | P68066 |
Family and domain databases
HAMAPi | MF_00806, GrcA, 1 hit |
InterProi | View protein in InterPro IPR019777, Form_AcTrfase_GR_CS IPR001150, Gly_radical IPR011140, Glycyl_radical_cofactor_GrcA |
Pfami | View protein in Pfam PF01228, Gly_radical, 1 hit |
PIRSFi | PIRSF000378, Gly_radicl_yfiD, 1 hit |
TIGRFAMsi | TIGR04365, spare_glycyl, 1 hit |
PROSITEi | View protein in PROSITE PS00850, GLY_RADICAL_1, 1 hit PS51149, GLY_RADICAL_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P68066-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MITGIQITKA ANDDLLNSFW LLDSEKGEAR CIVAKAGYAE DEVVAVSKLG
60 70 80 90 100
DIEYREVPVE VKPEVRVEGG QHLNVNVLRR ETLEDAVKHP EKYPQLTIRV
110 120
SGYAVRFNSL TPEQQRDVIA RTFTESL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13169 Genomic DNA No translation available. D64044 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC75632.1 AP009048 Genomic DNA Translation: BAA16465.1 J03725 Genomic DNA No translation available. |
PIRi | B65036 |
RefSeqi | NP_417074.1, NC_000913.3 WP_000627807.1, NZ_STEB01000011.1 |
Genome annotation databases
EnsemblBacteriai | AAC75632; AAC75632; b2579 BAA16465; BAA16465; BAA16465 |
GeneIDi | 66673531 947068 |
KEGGi | ecj:JW2563 eco:b2579 |
PATRICi | fig|1411691.4.peg.4155 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13169 Genomic DNA No translation available. D64044 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAC75632.1 AP009048 Genomic DNA Translation: BAA16465.1 J03725 Genomic DNA No translation available. |
PIRi | B65036 |
RefSeqi | NP_417074.1, NC_000913.3 WP_000627807.1, NZ_STEB01000011.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6OWR | NMR | - | A | 1-127 | [»] | |
AlphaFoldDBi | P68066 | |||||
SMRi | P68066 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263475, 8 interactors |
DIPi | DIP-47913N |
IntActi | P68066, 18 interactors |
STRINGi | 511145.b2579 |
PTM databases
iPTMneti | P68066 |
2D gel databases
SWISS-2DPAGEi | P68066 |
Proteomic databases
jPOSTi | P68066 |
PaxDbi | P68066 |
PRIDEi | P68066 |
Genome annotation databases
EnsemblBacteriai | AAC75632; AAC75632; b2579 BAA16465; BAA16465; BAA16465 |
GeneIDi | 66673531 947068 |
KEGGi | ecj:JW2563 eco:b2579 |
PATRICi | fig|1411691.4.peg.4155 |
Organism-specific databases
EchoBASEi | EB1732 |
Phylogenomic databases
eggNOGi | COG3445, Bacteria |
HOGENOMi | CLU_133780_0_0_6 |
OMAi | LGQFEYR |
PhylomeDBi | P68066 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11784-MONOMER |
Miscellaneous databases
PROi | PR:P68066 |
Family and domain databases
HAMAPi | MF_00806, GrcA, 1 hit |
InterProi | View protein in InterPro IPR019777, Form_AcTrfase_GR_CS IPR001150, Gly_radical IPR011140, Glycyl_radical_cofactor_GrcA |
Pfami | View protein in Pfam PF01228, Gly_radical, 1 hit |
PIRSFi | PIRSF000378, Gly_radicl_yfiD, 1 hit |
TIGRFAMsi | TIGR04365, spare_glycyl, 1 hit |
PROSITEi | View protein in PROSITE PS00850, GLY_RADICAL_1, 1 hit PS51149, GLY_RADICAL_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GRCA_ECOLI | |
Accessioni | P68066Primary (citable) accession number: P68066 Secondary accession number(s): P33633 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 21, 2004 |
Last sequence update: | December 21, 2004 | |
Last modified: | May 25, 2022 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references