UniProtKB - P68037 (UB2L3_MOUSE)
Ubiquitin-conjugating enzyme E2 L3
Ube2l3
Functioni
Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
By similarityCatalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 86 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- transcription coactivator activity Source: UniProtKB
- ubiquitin conjugating enzyme activity Source: MGI
- ubiquitin protein ligase binding Source: MGI
- ubiquitin-protein transferase activator activity Source: MGI
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- brain development Source: Ensembl
- cell cycle phase transition Source: UniProtKB
- cell population proliferation Source: UniProtKB
- cellular response to glucocorticoid stimulus Source: UniProtKB
- cellular response to steroid hormone stimulus Source: UniProtKB
- positive regulation of protein ubiquitination Source: MGI
- positive regulation of ubiquitin-protein transferase activity Source: MGI
- protein K11-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of transcription, DNA-templated Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: MGI
Keywordsi
Molecular function | Transferase |
Biological process | Transcription, Transcription regulation, Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-MMU-8866654, E3 ubiquitin ligases ubiquitinate target proteins R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 L3 (EC:2.3.2.23)Alternative name(s): E2 ubiquitin-conjugating enzyme L3 UbcM4 Ubiquitin carrier protein L3 Ubiquitin-protein ligase L3 |
Gene namesi | Name:Ube2l3 Synonyms:Ubce7 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:109240, Ube2l3 |
VEuPathDBi | HostDB:ENSMUSG00000038965 |
Subcellular locationi
Cytosol
- cytosol Source: Ensembl
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- presynapse Source: Ensembl
- ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082477 | 1 – 154 | Ubiquitin-conjugating enzyme E2 L3Add BLAST | 154 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 131 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
CPTACi | non-CPTAC-3679 |
EPDi | P68037 |
jPOSTi | P68037 |
PaxDbi | P68037 |
PeptideAtlasi | P68037 |
PRIDEi | P68037 |
ProteomicsDBi | 298349 |
PTM databases
iPTMneti | P68037 |
PhosphoSitePlusi | P68037 |
SwissPalmi | P68037 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000038965, Expressed in cerebellum and 312 other tissues |
ExpressionAtlasi | P68037, baseline and differential |
Genevisiblei | P68037, MM |
Interactioni
Subunit structurei
Interacts with PRKN; involved in ubiquitination and degradation of misfolded proteins.
Interacts with UBE3A.
Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B.
Interacts with ARIH1.
Interacts with ARIH2 (via RING-type 1).
Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity.
Interacts with NDFIP1 (via N-terminus); the interaction mediates recruitment of UBE2L3 to ITCH and causes MAP3K7 ubiquitination (PubMed:25632008).
By similarity1 PublicationGO - Molecular functioni
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 204407, 20 interactors |
IntActi | P68037, 1 interactor |
MINTi | P68037 |
STRINGi | 10090.ENSMUSP00000111363 |
Miscellaneous databases
RNActi | P68037, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 149 | UBC corePROSITE-ProRule annotationAdd BLAST | 148 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0422, Eukaryota |
GeneTreei | ENSGT00940000153654 |
HOGENOMi | CLU_030988_13_3_1 |
InParanoidi | P68037 |
OMAi | HPNITND |
OrthoDBi | 1420213at2759 |
PhylomeDBi | P68037 |
TreeFami | TF313043 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA
60 70 80 90 100
FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK
110 120 130 140 150
TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK
RPVD
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A338P7E5 | A0A338P7E5_MOUSE | Ubiquitin-conjugating enzyme E2 L3 | Ube2l3 | 122 | Annotation score: | ||
A0A338P702 | A0A338P702_MOUSE | Ubiquitin-conjugating enzyme E2 L3 | Ube2l3 | 114 | Annotation score: | ||
A0A338P786 | A0A338P786_MOUSE | Ubiquitin-conjugating enzyme E2 L3 | Ube2l3 | 146 | Annotation score: | ||
A0A338P6L1 | A0A338P6L1_MOUSE | Ubiquitin-conjugating enzyme E2 L3 | Ube2l3 | 32 | Annotation score: | ||
A0A338P724 | A0A338P724_MOUSE | Ubiquitin-conjugating enzyme E2 L3 | Ube2l3 | 26 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 | D → V in AAB36018 (PubMed:8901595).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S81004 Genomic DNA Translation: AAB36018.1 X97042 mRNA Translation: CAA65755.1 AJ130961 mRNA Translation: CAA10265.1 AK007265 mRNA Translation: BAB24925.1 |
CCDSi | CCDS27997.1 |
PIRi | JC6163 |
RefSeqi | NP_033482.1, NM_009456.2 |
Genome annotation databases
Ensembli | ENSMUST00000090192; ENSMUSP00000087658; ENSMUSG00000038965 ENSMUST00000232139; ENSMUSP00000156121; ENSMUSG00000038965 |
GeneIDi | 22195 |
KEGGi | mmu:22195 |
UCSCi | uc007ykl.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S81004 Genomic DNA Translation: AAB36018.1 X97042 mRNA Translation: CAA65755.1 AJ130961 mRNA Translation: CAA10265.1 AK007265 mRNA Translation: BAB24925.1 |
CCDSi | CCDS27997.1 |
PIRi | JC6163 |
RefSeqi | NP_033482.1, NM_009456.2 |
3D structure databases
AlphaFoldDBi | P68037 |
BMRBi | P68037 |
SMRi | P68037 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204407, 20 interactors |
IntActi | P68037, 1 interactor |
MINTi | P68037 |
STRINGi | 10090.ENSMUSP00000111363 |
PTM databases
iPTMneti | P68037 |
PhosphoSitePlusi | P68037 |
SwissPalmi | P68037 |
Proteomic databases
CPTACi | non-CPTAC-3679 |
EPDi | P68037 |
jPOSTi | P68037 |
PaxDbi | P68037 |
PeptideAtlasi | P68037 |
PRIDEi | P68037 |
ProteomicsDBi | 298349 |
Protocols and materials databases
DNASUi | 22195 |
Genome annotation databases
Ensembli | ENSMUST00000090192; ENSMUSP00000087658; ENSMUSG00000038965 ENSMUST00000232139; ENSMUSP00000156121; ENSMUSG00000038965 |
GeneIDi | 22195 |
KEGGi | mmu:22195 |
UCSCi | uc007ykl.1, mouse |
Organism-specific databases
CTDi | 7332 |
MGIi | MGI:109240, Ube2l3 |
VEuPathDBi | HostDB:ENSMUSG00000038965 |
Phylogenomic databases
eggNOGi | KOG0422, Eukaryota |
GeneTreei | ENSGT00940000153654 |
HOGENOMi | CLU_030988_13_3_1 |
InParanoidi | P68037 |
OMAi | HPNITND |
OrthoDBi | 1420213at2759 |
PhylomeDBi | P68037 |
TreeFami | TF313043 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-MMU-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-MMU-8866654, E3 ubiquitin ligases ubiquitinate target proteins R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 22195, 13 hits in 37 CRISPR screens |
ChiTaRSi | Ube2l3, mouse |
PROi | PR:P68037 |
RNActi | P68037, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000038965, Expressed in cerebellum and 312 other tissues |
ExpressionAtlasi | P68037, baseline and differential |
Genevisiblei | P68037, MM |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UB2L3_MOUSE | |
Accessioni | P68037Primary (citable) accession number: P68037 Secondary accession number(s): P51966, P70653, Q9HAV1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | May 25, 2022 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families