UniProtKB - P68036 (UB2L3_HUMAN)
Ubiquitin-conjugating enzyme E2 L3
UBE2L3
Functioni
Caution
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 86 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- enzyme binding Source: UniProtKB
- RNA binding Source: UniProtKB
- transcription coactivator activity Source: UniProtKB
- ubiquitin conjugating enzyme activity Source: MGI
- ubiquitin protein ligase binding Source: UniProtKB
- ubiquitin-protein transferase activator activity Source: ParkinsonsUK-UCL
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- cell cycle phase transition Source: UniProtKB
- cell population proliferation Source: UniProtKB
- cellular protein modification process Source: UniProtKB
- cellular response to glucocorticoid stimulus Source: UniProtKB
- cellular response to steroid hormone stimulus Source: UniProtKB
- positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
- positive regulation of protein ubiquitination Source: ParkinsonsUK-UCL
- positive regulation of ubiquitin-protein transferase activity Source: ParkinsonsUK-UCL
- protein K11-linked ubiquitination Source: UniProtKB
- protein polyubiquitination Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of transcription, DNA-templated Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Transcription, Transcription regulation, Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.3.2.B6, 2681 |
PathwayCommonsi | P68036 |
Reactomei | R-HSA-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-HSA-8866654, E3 ubiquitin ligases ubiquitinate target proteins R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | P68036 |
UniPathwayi | UPA00143 |
Protein family/group databases
MoonDBi | P68036, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 L3 (EC:2.3.2.231 Publication)Alternative name(s): E2 ubiquitin-conjugating enzyme L3 L-UBC UbcH7 Ubiquitin carrier protein L3 Ubiquitin-conjugating enzyme E2-F1 Ubiquitin-protein ligase L3 |
Gene namesi | Name:UBE2L3 Synonyms:UBCE7, UBCH7 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000185651.14 |
HGNCi | HGNC:12488, UBE2L3 |
MIMi | 603721, gene |
neXtProti | NX_P68036 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 9 | K → E: Marked decrease in autoubiquitination. 1 Publication | 1 | |
Mutagenesisi | 63 | F → A: Decrease in autoubiquitination. 1 Publication | 1 | |
Mutagenesisi | 86 | C → S: Loss of catalytic activity. Prevents ubiquitin-dependent proteasomal degradation of UBE2L3. 2 Publications | 1 | |
Mutagenesisi | 88 | P → D: Does not convert into a lysine reactive E2; when associated with D-119. 1 Publication | 1 | |
Mutagenesisi | 93 | E → R: Decrease in autoubiquitination. 1 Publication | 1 | |
Mutagenesisi | 96 | K → E: Decrease in autoubiquitination. 1 Publication | 1 | |
Mutagenesisi | 100 | K → E: Decrease in autoubiquitination. 1 Publication | 1 | |
Mutagenesisi | 119 | H → D: Does not convert into a lysine reactive E2; when associated with D-88. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 7332 |
MalaCardsi | UBE2L3 |
OpenTargetsi | ENSG00000185651 |
Orphaneti | 536, Systemic lupus erythematosus |
PharmGKBi | PA37137 |
Miscellaneous databases
Pharosi | P68036, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105871 |
Polymorphism and mutation databases
BioMutai | UBE2L3 |
DMDMi | 54039805 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082476 | 1 – 154 | Ubiquitin-conjugating enzyme E2 L3Add BLAST | 154 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 131 | N6-acetyllysineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
EPDi | P68036 |
jPOSTi | P68036 |
MassIVEi | P68036 |
MaxQBi | P68036 |
PaxDbi | P68036 |
PeptideAtlasi | P68036 |
PRIDEi | P68036 |
ProteomicsDBi | 18904 3856 57528 [P68036-1] |
TopDownProteomicsi | P68036-1 [P68036-1] P68036-2 [P68036-2] |
2D gel databases
OGPi | P51966 |
UCD-2DPAGEi | P68036 |
PTM databases
iPTMneti | P68036 |
PhosphoSitePlusi | P68036 |
SwissPalmi | P68036 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000185651, Expressed in C1 segment of cervical spinal cord and 239 other tissues |
ExpressionAtlasi | P68036, baseline and differential |
Genevisiblei | P68036, HS |
Organism-specific databases
HPAi | ENSG00000185651, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with PRKN; involved in ubiquitination and degradation of misfolded proteins.
Interacts with UBE3A; used by the papilloma virus HPV-16 E6 protein to ubiquitinate p53/TP53.
Interacts with CCNB1IP1, CBL, ZAP70, RNF19A, RNF19B and RNF144B.
Interacts with ARIH1.
Interacts with ARIH2 (via RING-type 1).
Interacts with NCOA1; they functionally interact to regulate progesterone receptor transcriptional activity. May interact with NR3C1.
Interacts with NDFIP1 (via N-terminus); the interaction mediates recruitment of UBE2L3 to ITCH and causes MAP3K7 ubiquitination (PubMed:25632008).
13 PublicationsBinary interactionsi
Hide detailsP68036
Isoform 1 [P68036-1]
GO - Molecular functioni
- enzyme binding Source: UniProtKB
- ubiquitin protein ligase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 113180, 193 interactors |
CORUMi | P68036 |
DIPi | DIP-6124N |
IntActi | P68036, 35 interactors |
MINTi | P68036 |
STRINGi | 9606.ENSP00000485133 |
Miscellaneous databases
RNActi | P68036, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | P68036 |
SMRi | P68036 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P68036 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 149 | UBC corePROSITE-ProRule annotationAdd BLAST | 148 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0422, Eukaryota |
GeneTreei | ENSGT00940000153654 |
HOGENOMi | CLU_030988_13_3_1 |
InParanoidi | P68036 |
OMAi | YRRAHGP |
OrthoDBi | 1420213at2759 |
PhylomeDBi | P68036 |
TreeFami | TF313043 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
IDEALi | IID00592 |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAASRRLMKE LEEIRKCGMK NFRNIQVDEA NLLTWQGLIV PDNPPYDKGA
60 70 80 90 100
FRIEINFPAE YPFKPPKITF KTKIYHPNID EKGQVCLPVI SAENWKPATK
110 120 130 140 150
TDQVIQSLIA LVNDPQPEHP LRADLAEEYS KDRKKFCKNA EEFTKKYGEK
RPVD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 15 | R → S in BAG61806 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 23 | R → C in AAG17922 (Ref. 4) Curated | 1 | |
Sequence conflicti | 118 | E → K in AAG17922 (Ref. 4) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047342 | 1 – 9 | MAASRRLMK → MQVAAGTRGDTRLQEVALLP QLFDLLVLGQRRARLLRQVP SALAGKDLAQLQAGATLAGY RRAHGPE in isoform 3. 1 Publication | 9 | |
Alternative sequenceiVSP_045152 | 10 – 41 | Missing in isoform 2. 1 PublicationAdd BLAST | 32 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S81003 mRNA Translation: AAB36017.1 X92962 mRNA Translation: CAA63538.1 AJ000519 mRNA Translation: CAA04156.1 AF300336 Genomic DNA Translation: AAG17922.1 CR456606 mRNA Translation: CAG30492.1 AK293179 mRNA Translation: BAG56722.1 AK299985 mRNA Translation: BAG61806.1 AK311761 mRNA Translation: BAG34704.1 AP000553 Genomic DNA No translation available. AP000557 Genomic DNA No translation available. AP000558 Genomic DNA No translation available. CH471095 Genomic DNA Translation: EAW59459.1 BC053368 mRNA Translation: AAH53368.1 |
CCDSi | CCDS13790.1 [P68036-1] CCDS58795.1 [P68036-3] CCDS58796.1 [P68036-2] |
RefSeqi | NP_001243284.1, NM_001256355.1 [P68036-3] NP_001243285.1, NM_001256356.1 [P68036-2] NP_003338.1, NM_003347.3 [P68036-1] |
Genome annotation databases
Ensembli | ENST00000342192; ENSP00000344259; ENSG00000185651 [P68036-1] ENST00000458578; ENSP00000400906; ENSG00000185651 [P68036-3] ENST00000545681; ENSP00000445931; ENSG00000185651 [P68036-2] |
GeneIDi | 7332 |
KEGGi | hsa:7332 |
UCSCi | uc002zva.2, human [P68036-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S81003 mRNA Translation: AAB36017.1 X92962 mRNA Translation: CAA63538.1 AJ000519 mRNA Translation: CAA04156.1 AF300336 Genomic DNA Translation: AAG17922.1 CR456606 mRNA Translation: CAG30492.1 AK293179 mRNA Translation: BAG56722.1 AK299985 mRNA Translation: BAG61806.1 AK311761 mRNA Translation: BAG34704.1 AP000553 Genomic DNA No translation available. AP000557 Genomic DNA No translation available. AP000558 Genomic DNA No translation available. CH471095 Genomic DNA Translation: EAW59459.1 BC053368 mRNA Translation: AAH53368.1 |
CCDSi | CCDS13790.1 [P68036-1] CCDS58795.1 [P68036-3] CCDS58796.1 [P68036-2] |
RefSeqi | NP_001243284.1, NM_001256355.1 [P68036-3] NP_001243285.1, NM_001256356.1 [P68036-2] NP_003338.1, NM_003347.3 [P68036-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1C4Z | X-ray | 2.60 | D | 1-154 | [»] | |
1FBV | X-ray | 2.90 | C | 1-154 | [»] | |
3SQV | X-ray | 3.30 | C/D | 1-154 | [»] | |
3SY2 | X-ray | 3.27 | C/D | 1-154 | [»] | |
4Q5E | X-ray | 1.87 | C | 1-154 | [»] | |
4Q5H | X-ray | 2.00 | C | 1-154 | [»] | |
5HPT | X-ray | 2.84 | C/F | 2-154 | [»] | |
5TTE | X-ray | 3.50 | E | 1-154 | [»] | |
5UDH | X-ray | 3.24 | C/D | 1-154 | [»] | |
6CP2 | X-ray | 2.90 | B | 1-154 | [»] | |
6DJW | X-ray | 3.80 | C | 1-154 | [»] | |
6DJX | X-ray | 4.80 | C | 1-154 | [»] | |
6N13 | NMR | - | C | 1-154 | [»] | |
6XXU | NMR | - | A | 1-154 | [»] | |
BMRBi | P68036 | |||||
SMRi | P68036 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 113180, 193 interactors |
CORUMi | P68036 |
DIPi | DIP-6124N |
IntActi | P68036, 35 interactors |
MINTi | P68036 |
STRINGi | 9606.ENSP00000485133 |
Chemistry databases
ChEMBLi | CHEMBL4105871 |
Protein family/group databases
MoonDBi | P68036, Predicted |
PTM databases
iPTMneti | P68036 |
PhosphoSitePlusi | P68036 |
SwissPalmi | P68036 |
Polymorphism and mutation databases
BioMutai | UBE2L3 |
DMDMi | 54039805 |
2D gel databases
OGPi | P51966 |
UCD-2DPAGEi | P68036 |
Proteomic databases
EPDi | P68036 |
jPOSTi | P68036 |
MassIVEi | P68036 |
MaxQBi | P68036 |
PaxDbi | P68036 |
PeptideAtlasi | P68036 |
PRIDEi | P68036 |
ProteomicsDBi | 18904 3856 57528 [P68036-1] |
TopDownProteomicsi | P68036-1 [P68036-1] P68036-2 [P68036-2] |
Protocols and materials databases
Antibodypediai | 8540, 443 antibodies |
DNASUi | 7332 |
Genome annotation databases
Ensembli | ENST00000342192; ENSP00000344259; ENSG00000185651 [P68036-1] ENST00000458578; ENSP00000400906; ENSG00000185651 [P68036-3] ENST00000545681; ENSP00000445931; ENSG00000185651 [P68036-2] |
GeneIDi | 7332 |
KEGGi | hsa:7332 |
UCSCi | uc002zva.2, human [P68036-1] |
Organism-specific databases
CTDi | 7332 |
DisGeNETi | 7332 |
EuPathDBi | HostDB:ENSG00000185651.14 |
GeneCardsi | UBE2L3 |
HGNCi | HGNC:12488, UBE2L3 |
HPAi | ENSG00000185651, Low tissue specificity |
MalaCardsi | UBE2L3 |
MIMi | 603721, gene |
neXtProti | NX_P68036 |
OpenTargetsi | ENSG00000185651 |
Orphaneti | 536, Systemic lupus erythematosus |
PharmGKBi | PA37137 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0422, Eukaryota |
GeneTreei | ENSGT00940000153654 |
HOGENOMi | CLU_030988_13_3_1 |
InParanoidi | P68036 |
OMAi | YRRAHGP |
OrthoDBi | 1420213at2759 |
PhylomeDBi | P68036 |
TreeFami | TF313043 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 2.3.2.B6, 2681 |
PathwayCommonsi | P68036 |
Reactomei | R-HSA-8866652, Synthesis of active ubiquitin: roles of E1 and E2 enzymes R-HSA-8866654, E3 ubiquitin ligases ubiquitinate target proteins R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | P68036 |
Miscellaneous databases
BioGRID-ORCSi | 7332, 624 hits in 821 CRISPR screens |
ChiTaRSi | UBE2L3, human |
EvolutionaryTracei | P68036 |
GeneWikii | UBE2L3 |
GenomeRNAii | 7332 |
Pharosi | P68036, Tbio |
PROi | PR:P68036 |
RNActi | P68036, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000185651, Expressed in C1 segment of cervical spinal cord and 239 other tissues |
ExpressionAtlasi | P68036, baseline and differential |
Genevisiblei | P68036, HS |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
IDEALi | IID00592 |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UB2L3_HUMAN | |
Accessioni | P68036Primary (citable) accession number: P68036 Secondary accession number(s): B2R4A7 Q9HAV1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM