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Protein

Tropomyosin alpha-4 chain

Gene

TPM4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments (By similarity). Binds calcium (PubMed:1836432).By similarity1 Publication

GO - Molecular functioni

  • actin filament binding Source: UniProtKB
  • calcium ion binding Source: UniProtKB
  • identical protein binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • structural constituent of muscle Source: ProtInc

GO - Biological processi

  • actin filament organization Source: GO_Central
  • muscle contraction Source: Reactome
  • muscle filament sliding Source: Reactome
  • osteoblast differentiation Source: UniProtKB

Keywordsi

Molecular functionActin-binding, Muscle protein
LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-390522 Striated Muscle Contraction
R-HSA-445355 Smooth Muscle Contraction

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin alpha-4 chain
Alternative name(s):
TM30p1
Tropomyosin-4
Gene namesi
Name:TPM4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000167460.14
HGNCiHGNC:12013 TPM4
MIMi600317 gene
neXtProtiNX_P67936

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

DisGeNETi7171
MalaCardsiTPM4
OpenTargetsiENSG00000167460
Orphaneti140957 Autosomal dominant macrothrombocytopenia
178342 Inflammatory myofibroblastic tumor
PharmGKBiPA36693

Polymorphism and mutation databases

BioMutaiTPM4
DMDMi54039751

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources2 Publications
ChainiPRO_00002056352 – 248Tropomyosin alpha-4 chainAdd BLAST247

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei6PhosphoserineBy similarity1
Modified residuei177N6-acetyllysineCombined sources1
Modified residuei215N6-acetyllysineCombined sources1
Modified residuei216PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP67936
PeptideAtlasiP67936
PRIDEiP67936
ProteomicsDBi57525
57526 [P67936-2]

2D gel databases

DOSAC-COBS-2DPAGEiP67936
OGPiP07226

PTM databases

iPTMnetiP67936
PhosphoSitePlusiP67936
SwissPalmiP67936

Expressioni

Tissue specificityi

Detected in cardiac tissue and platelets, the form found in cardiac tissue is a higher molecular weight than the form found in platelets. Expressed at higher levels in the platelets of hypertensive patients with cardiac hypertrophy than in the platelets of hypertensive patients without cardiac hypertrophy (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000167460 Expressed in 190 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_TPM4
ExpressionAtlasiP67936 baseline and differential
GenevisibleiP67936 HS

Organism-specific databases

HPAiCAB004685
HPA047089
HPA053624

Interactioni

Subunit structurei

Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PTP4A3O75365-22EBI-1642100,EBI-14013960

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113024, 95 interactors
IntActiP67936, 61 interactors

Structurei

3D structure databases

ProteinModelPortaliP67936
SMRiP67936
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili2 – 248By similarityAdd BLAST247

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074494
HOVERGENiHBG107404
InParanoidiP67936
KOiK10375
OMAiDKYKAIC
OrthoDBiEOG091G0UO7
PhylomeDBiP67936
TreeFamiTF351519

Family and domain databases

InterProiView protein in InterPro
IPR000533 Tropomyosin
PfamiView protein in Pfam
PF00261 Tropomyosin, 1 hit
PRINTSiPR00194 TROPOMYOSIN
PROSITEiView protein in PROSITE
PS00326 TROPOMYOSIN, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 14 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P67936-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAGLNSLEAV KRKIQALQQQ ADEAEDRAQG LQRELDGERE RREKAEGDVA
60 70 80 90 100
ALNRRIQLVE EELDRAQERL ATALQKLEEA EKAADESERG MKVIENRAMK
110 120 130 140 150
DEEKMEIQEM QLKEAKHIAE EADRKYEEVA RKLVILEGEL ERAEERAEVS
160 170 180 190 200
ELKCGDLEEE LKNVTNNLKS LEAASEKYSE KEDKYEEEIK LLSDKLKEAE
210 220 230 240
TRAEFAERTV AKLEKTIDDL EEKLAQAKEE NVGLHQTLDQ TLNELNCI
Length:248
Mass (Da):28,522
Last modified:January 23, 2007 - v3
Checksum:i5D5CDF6BF76552A8
GO
Isoform 2 (identifier: P67936-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: MAGLNSLEAV...LDGERERREK → MEAIKKKMQM...LELTEKKASD

Show »
Length:284
Mass (Da):32,723
Checksum:i29C0F6ADF4C87F0C
GO

Computationally mapped potential isoform sequencesi

There are 14 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
K7ENT6K7ENT6_HUMAN
Tropomyosin alpha-4 chain
TPM4
247Annotation score:
K7EPB9K7EPB9_HUMAN
Tropomyosin alpha-4 chain
TPM4
110Annotation score:
A0A2R8Y5V9A0A2R8Y5V9_HUMAN
Tropomyosin alpha-4 chain
TPM4
248Annotation score:
A0A2R8YE05A0A2R8YE05_HUMAN
Tropomyosin alpha-4 chain
TPM4
226Annotation score:
A0A2R8YH90A0A2R8YH90_HUMAN
Tropomyosin alpha-4 chain
TPM4
220Annotation score:
K7ERG3K7ERG3_HUMAN
Tropomyosin alpha-4 chain
TPM4
170Annotation score:
K7EPV9K7EPV9_HUMAN
Tropomyosin alpha-4 chain
TPM4
96Annotation score:
K7EMU5K7EMU5_HUMAN
Tropomyosin alpha-4 chain
TPM4
102Annotation score:
K7EP68K7EP68_HUMAN
Tropomyosin alpha-4 chain
TPM4
138Annotation score:
K7ELP0K7ELP0_HUMAN
Tropomyosin alpha-4 chain
TPM4
69Annotation score:
There are more potential isoformsShow all

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59V → F in AAH02827 (PubMed:15489334).Curated1
Sequence conflicti152L → S in AAA36774 (PubMed:3865200).Curated1
Sequence conflicti162 – 163KN → RT in AAA36774 (PubMed:3865200).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036535204E → Q in a breast cancer sample; somatic mutation. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0066111 – 44MAGLN…ERREK → MEAIKKKMQMLKLDKENAID RAEQAEADKKAAEDKCKQVE EELTHLQKKLKGTEDELDKY SEDLKDAQEKLELTEKKASD in isoform 2. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05276 mRNA Translation: CAA28888.1
AK023385 mRNA Translation: BAB14554.1
AK315076 mRNA Translation: BAG37545.1
BT019634 mRNA Translation: AAV38440.1
CH471106 Genomic DNA Translation: EAW84521.1
BC002827 mRNA Translation: AAH02827.1
BC037576 mRNA Translation: AAH37576.1
BC067225 mRNA Translation: AAH67225.1
M12127 mRNA Translation: AAA36774.1
CCDSiCCDS12338.1
CCDS46007.1 [P67936-2]
PIRiS07282
RefSeqiNP_001138632.1, NM_001145160.1 [P67936-2]
NP_003281.1, NM_003290.2 [P67936-1]
UniGeneiHs.631618
Hs.665133

Genome annotation databases

EnsembliENST00000300933; ENSP00000300933; ENSG00000167460 [P67936-1]
ENST00000344824; ENSP00000345230; ENSG00000167460 [P67936-2]
ENST00000643579; ENSP00000495347; ENSG00000167460 [P67936-1]
ENST00000646974; ENSP00000494125; ENSG00000167460 [P67936-2]
GeneIDi7171
KEGGihsa:7171
UCSCiuc002ndi.3 human

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05276 mRNA Translation: CAA28888.1
AK023385 mRNA Translation: BAB14554.1
AK315076 mRNA Translation: BAG37545.1
BT019634 mRNA Translation: AAV38440.1
CH471106 Genomic DNA Translation: EAW84521.1
BC002827 mRNA Translation: AAH02827.1
BC037576 mRNA Translation: AAH37576.1
BC067225 mRNA Translation: AAH67225.1
M12127 mRNA Translation: AAA36774.1
CCDSiCCDS12338.1
CCDS46007.1 [P67936-2]
PIRiS07282
RefSeqiNP_001138632.1, NM_001145160.1 [P67936-2]
NP_003281.1, NM_003290.2 [P67936-1]
UniGeneiHs.631618
Hs.665133

3D structure databases

ProteinModelPortaliP67936
SMRiP67936
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113024, 95 interactors
IntActiP67936, 61 interactors

PTM databases

iPTMnetiP67936
PhosphoSitePlusiP67936
SwissPalmiP67936

Polymorphism and mutation databases

BioMutaiTPM4
DMDMi54039751

2D gel databases

DOSAC-COBS-2DPAGEiP67936
OGPiP07226

Proteomic databases

EPDiP67936
PeptideAtlasiP67936
PRIDEiP67936
ProteomicsDBi57525
57526 [P67936-2]

Protocols and materials databases

DNASUi7171
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300933; ENSP00000300933; ENSG00000167460 [P67936-1]
ENST00000344824; ENSP00000345230; ENSG00000167460 [P67936-2]
ENST00000643579; ENSP00000495347; ENSG00000167460 [P67936-1]
ENST00000646974; ENSP00000494125; ENSG00000167460 [P67936-2]
GeneIDi7171
KEGGihsa:7171
UCSCiuc002ndi.3 human

Organism-specific databases

CTDi7171
DisGeNETi7171
EuPathDBiHostDB:ENSG00000167460.14
GeneCardsiTPM4
H-InvDBiHIX0022609
HGNCiHGNC:12013 TPM4
HPAiCAB004685
HPA047089
HPA053624
MalaCardsiTPM4
MIMi600317 gene
neXtProtiNX_P67936
OpenTargetsiENSG00000167460
Orphaneti140957 Autosomal dominant macrothrombocytopenia
178342 Inflammatory myofibroblastic tumor
PharmGKBiPA36693
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00550000074494
HOVERGENiHBG107404
InParanoidiP67936
KOiK10375
OMAiDKYKAIC
OrthoDBiEOG091G0UO7
PhylomeDBiP67936
TreeFamiTF351519

Enzyme and pathway databases

ReactomeiR-HSA-390522 Striated Muscle Contraction
R-HSA-445355 Smooth Muscle Contraction

Miscellaneous databases

ChiTaRSiTPM4 human
GeneWikiiTPM4
GenomeRNAii7171
PROiPR:P67936
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167460 Expressed in 190 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_TPM4
ExpressionAtlasiP67936 baseline and differential
GenevisibleiP67936 HS

Family and domain databases

InterProiView protein in InterPro
IPR000533 Tropomyosin
PfamiView protein in Pfam
PF00261 Tropomyosin, 1 hit
PRINTSiPR00194 TROPOMYOSIN
PROSITEiView protein in PROSITE
PS00326 TROPOMYOSIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPM4_HUMAN
AccessioniPrimary (citable) accession number: P67936
Secondary accession number(s): P07226
, Q15659, Q5U0D9, Q9BU85, Q9H8Q3, Q9UCS1, Q9UCS2, Q9UCS3, Q9UCS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
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