UniProtKB - P67827 (KC1A_BOVIN)
Protein
Casein kinase I isoform alpha
Gene
CSNK1A1
Organism
Bos taurus (Bovine)
Status
Functioni
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.By similarity
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 46 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 136 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 23 – 31 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: UniProtKB
- protein serine/threonine kinase activity Source: AgBase
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- intermediate filament cytoskeleton organization Source: UniProtKB
- negative regulation of canonical Wnt signaling pathway Source: GO_Central
- peptidyl-serine phosphorylation Source: AgBase
- protein phosphorylation Source: UniProtKB
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Mitosis, Wnt signaling pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 908 |
Names & Taxonomyi
Protein namesi | Recommended name: Casein kinase I isoform alpha (EC:2.7.11.1)Short name: CKI-alpha Alternative name(s): CK1 |
Gene namesi | Name:CSNK1A1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:55205, CSNK1A1 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
- cilium basal body By similarity
Nucleus
- Nucleus speckle By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- kinetochore By similarity
Note: Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis. Also recruited to the keratin cytoskeleton.By similarity
Cytoskeleton
- ciliary basal body Source: UniProtKB
Golgi apparatus
- Golgi membrane Source: AgBase
Nucleus
- nuclear speck Source: UniProtKB
- nucleus Source: GO_Central
Other locations
- condensed chromosome kinetochore Source: UniProtKB-SubCell
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cell projection, Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000192821 | 2 – 325 | Casein kinase I isoform alphaAdd BLAST | 324 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 4 | PhosphoserineBy similarity | 1 | |
Modified residuei | 8 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | P67827 |
PRIDEi | P67827 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000005326, Expressed in longissimus thoracis muscle and 18 other tissues |
ExpressionAtlasi | P67827, baseline |
Interactioni
Subunit structurei
Monomer.
Interacts with the Axin complex.
Interacts with TUT1, leading to TUT1 phosphorylation.
Interacts with FAM83H; recruits CSNK1A1 to keratin filaments.
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000035028 |
Chemistry databases
BindingDBi | P67827 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 285 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 269 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1163, Eukaryota |
GeneTreei | ENSGT00940000153700 |
HOGENOMi | CLU_019279_2_7_1 |
InParanoidi | P67827 |
OrthoDBi | 1097975at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P67827-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASSSGSKAE FIVGGKYKLV RKIGSGSFGD IYLAINITNG EEVAVKLESQ
60 70 80 90 100
KARHPQLLYE SKLYKILQGG VGIPHIRWYG QEKDYNVLVM DLLGPSLEDL
110 120 130 140 150
FNFCSRRFTM KTVLMLADQM ISRIEYVHTK NFIHRDIKPD NFLMGIGRHC
160 170 180 190 200
NKLFLIDFGL AKKYRDNRTR QHIPYREDKN LTGTARYASI NAHLGIEQSR
210 220 230 240 250
RDDMESLGYV LMYFNRTSLP WQGLKAATKK QKYEKISEKK MSTPVEVLCK
260 270 280 290 300
GFPAEFAMYL NYCRGLRFEE APDYMYLRQL FRILFRTLNH QYDYTFDWTM
310 320
LKQKAAQQAA SSSGQGQQAQ TPTGF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ32LI4 | Q32LI4_BOVIN | CSNK1A1 protein | CSNK1A1 | 334 | Annotation score: | ||
A0A3Q1LK11 | A0A3Q1LK11_BOVIN | Casein kinase I isoform alpha | CSNK1A1 | 380 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | A → G in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 2 | A → G in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 2 | A → G in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 24 | G → R in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 24 | G → R in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 24 | G → R in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 31 | I → M in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 31 | I → M in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 31 | I → M in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 64 | Y → C in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 64 | Y → C in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 64 | Y → C in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 70 | G → E in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 70 | G → E in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 70 | G → E in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 82 | E → Q in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 82 | E → Q in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 82 | E → Q in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 121 – 122 | IS → MG in BAB17767 (Ref. 2) Curated | 2 | |
Sequence conflicti | 121 – 122 | IS → MG in BAB17768 (Ref. 2) Curated | 2 | |
Sequence conflicti | 191 | N → S in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 191 | N → S in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 191 | N → S in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 279 | Q → H in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 279 | Q → H in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 279 | Q → H in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 282 | R → H in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 282 | R → H in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 282 | R → H in BAB17806 (Ref. 2) Curated | 1 | |
Sequence conflicti | 296 | F → S in BAB17767 (Ref. 2) Curated | 1 | |
Sequence conflicti | 296 | F → S in BAB17768 (Ref. 2) Curated | 1 | |
Sequence conflicti | 296 | F → S in BAB17806 (Ref. 2) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 60 | E → Q in strain: Holstein. | 1 | |
Natural varianti | 67 | L → P in strain: Jersey. | 1 | |
Natural varianti | 96 | S → T in strain: Hereford. | 1 | |
Natural varianti | 216 | R → T in strain: Jersey. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76543 mRNA Translation: AAA30451.1 AB050943 Genomic DNA Translation: BAB17767.1 AB050944 Genomic DNA Translation: BAB17768.1 AB050946 Genomic DNA Translation: BAB17806.1 |
PIRi | A56406 |
RefSeqi | NP_777136.1, NM_174711.2 |
Genome annotation databases
Ensembli | ENSBTAT00000007007; ENSBTAP00000007007; ENSBTAG00000005326 |
GeneIDi | 282684 |
KEGGi | bta:282684 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76543 mRNA Translation: AAA30451.1 AB050943 Genomic DNA Translation: BAB17767.1 AB050944 Genomic DNA Translation: BAB17768.1 AB050946 Genomic DNA Translation: BAB17806.1 |
PIRi | A56406 |
RefSeqi | NP_777136.1, NM_174711.2 |
3D structure databases
SMRi | P67827 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000035028 |
Chemistry databases
BindingDBi | P67827 |
ChEMBLi | CHEMBL3337329 |
Proteomic databases
PaxDbi | P67827 |
PRIDEi | P67827 |
Genome annotation databases
Ensembli | ENSBTAT00000007007; ENSBTAP00000007007; ENSBTAG00000005326 |
GeneIDi | 282684 |
KEGGi | bta:282684 |
Organism-specific databases
CTDi | 1452 |
VGNCi | VGNC:55205, CSNK1A1 |
Phylogenomic databases
eggNOGi | KOG1163, Eukaryota |
GeneTreei | ENSGT00940000153700 |
HOGENOMi | CLU_019279_2_7_1 |
InParanoidi | P67827 |
OrthoDBi | 1097975at2759 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 908 |
Gene expression databases
Bgeei | ENSBTAG00000005326, Expressed in longissimus thoracis muscle and 18 other tissues |
ExpressionAtlasi | P67827, baseline |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KC1A_BOVIN | |
Accessioni | P67827Primary (citable) accession number: P67827 Secondary accession number(s): P35506 Q9GLY3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families