UniProtKB - P67779 (PHB1_RAT)
Prohibitin 1
Phb1
Functioni
Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus. Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner (By similarity) (PubMed:1996099).
Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells (PubMed:32173312).
By similarity2 PublicationsIn the mitochondria, together with PHB2, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan. The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner. Regulates mitochondrial respiration activity playing a role in cellular aging. The prohibitin complex plays a role of mitophagy receptor involved in targeting mitochondria for autophagic degradation (By similarity).
Involved in mitochondrial-mediated antiviral innate immunity, activates DDX58/RIG-I-mediated signal transduction and production of IFNB1 and proinflammatory cytokine IL6 (By similarity).
By similarityIn the nucleus, acts as a transcription coregulator, enhances promoter binding by TP53, a transcription factor it activates, but reduces the promoter binding by E2F1, a transcription factor it represses. Interacts with STAT3 to affect IL17 secretion in T-helper Th17 cells.
By similarityIn the plasma membrane, cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates. Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.
By similarityGO - Molecular functioni
- complement component C3a binding Source: RGD
- complement component C3b binding Source: RGD
- enzyme binding Source: RGD
- histone deacetylase binding Source: RGD
- proteinase activated receptor binding Source: RGD
- protein C-terminus binding Source: RGD
- protein heterodimerization activity Source: RGD
- transcription corepressor activity Source: RGD
GO - Biological processi
- activation of phospholipase C activity Source: UniProtKB
- activation of protein kinase C activity Source: UniProtKB
- animal organ regeneration Source: RGD
- antiviral innate immune response Source: RGD
- B cell activation Source: UniProtKB
- CD40 signaling pathway Source: UniProtKB
- cellular response to interleukin-6 Source: RGD
- DNA biosynthetic process Source: RGD
- histone deacetylation Source: RGD
- mitochondrion organization Source: RGD
- modulation by host of viral RNA genome replication Source: RGD
- negative regulation of androgen receptor signaling pathway Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of cell growth Source: RGD
- negative regulation of cell population proliferation Source: RGD
- negative regulation of ERK1 and ERK2 cascade Source: RGD
- negative regulation of glucocorticoid receptor signaling pathway Source: RGD
- negative regulation of protein catabolic process Source: RGD
- negative regulation of transcription, DNA-templated Source: RGD
- negative regulation of transcription by competitive promoter binding Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- ovarian follicle atresia Source: RGD
- ovarian follicle development Source: RGD
- positive regulation of cell death Source: RGD
- positive regulation of complement activation Source: RGD
- positive regulation of ERK1 and ERK2 cascade Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of G protein-coupled receptor signaling pathway Source: RGD
- positive regulation of immunoglobulin production Source: UniProtKB
- positive regulation of interleukin-17 production Source: UniProtKB
- positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
- positive regulation of protein kinase B signaling Source: UniProtKB
- positive regulation of smooth muscle cell proliferation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: RGD
- progesterone receptor signaling pathway Source: RGD
- protein stabilization Source: RGD
- regulation of transcription, DNA-templated Source: RGD
- response to cytokine Source: RGD
- response to ethanol Source: RGD
- response to immobilization stress Source: RGD
- response to peptide hormone Source: RGD
- response to xenobiotic stimulus Source: RGD
- RIG-I signaling pathway Source: RGD
- T-helper 17 type immune response Source: UniProtKB
- viral entry into host cell Source: RGD
Keywordsi
Biological process | DNA synthesis |
Enzyme and pathway databases
Reactomei | R-RNO-5673000, RAF activation R-RNO-8949664, Processing of SMDT1 |
Names & Taxonomyi
Protein namesi | Recommended name: Prohibitin 1 |
Gene namesi | Name:Phb1Imported Synonyms:Phb |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3322, Phb |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Nucleus
- Nucleus By similarity
Mitochondrion
- Mitochondrion inner membrane 1 Publication
Endosome
- early endosome Source: RGD
Mitochondrion
- extrinsic component of mitochondrial outer membrane Source: RGD
- mitochondrial crista Source: RGD
- mitochondrial inner membrane Source: RGD
- mitochondrial prohibitin complex Source: UniProtKB
- mitochondrion Source: RGD
Nucleus
- nucleoplasm Source: RGD
- nucleus Source: RGD
Plasma Membrane
- extrinsic component of presynaptic active zone membrane Source: SynGO
- integral component of plasma membrane Source: RGD
- plasma membrane Source: UniProtKB
Other locations
- cell surface Source: UniProtKB
- cytoplasm Source: RGD
- GABA-ergic synapse Source: SynGO
- glutamatergic synapse Source: SynGO
- postsynaptic density Source: SynGO
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000213880 | 2 – 272 | Prohibitin 1Add BLAST | 271 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 91 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 128 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 186 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 202 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 202 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 249 | PhosphotyrosineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
jPOSTi | P67779 |
PaxDbi | P67779 |
PRIDEi | P67779 |
2D gel databases
World-2DPAGEi | 0004:P67779 |
PTM databases
iPTMneti | P67779 |
PhosphoSitePlusi | P67779 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000046799, Expressed in heart and 22 other tissues |
Genevisiblei | P67779, RN |
Interactioni
Subunit structurei
The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2), assembled into a membrane-associated ring-shaped supercomplex of approximately 1 mDa.
Interacts with STOML2.
Interacts with MAP1LC3B (membrane-bound form LC3-II); the interaction requires PHB2 and takes place upon Parkin-mediated mitochondrial damage.
Interacts with STAT3 (unphosphorylated or phosphorylated at 'Ser-727').
Interacts with CLPB (By similarity).
Interacts with CD86 (via cytoplasmic domain); the interactions increases after priming with CD40 (By similarity).
By similarityGO - Molecular functioni
- complement component C3a binding Source: RGD
- complement component C3b binding Source: RGD
- enzyme binding Source: RGD
- histone deacetylase binding Source: RGD
- proteinase activated receptor binding Source: RGD
- protein C-terminus binding Source: RGD
- protein heterodimerization activity Source: RGD
Protein-protein interaction databases
BioGRIDi | 247383, 5 interactors |
IntActi | P67779, 4 interactors |
MINTi | P67779 |
STRINGi | 10116.ENSRNOP00000066502 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 177 – 211 | Sequence analysisAdd BLAST | 35 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG3083, Eukaryota |
GeneTreei | ENSGT00950000183070 |
HOGENOMi | CLU_047969_0_0_1 |
InParanoidi | P67779 |
OMAi | RTRIRDN |
OrthoDBi | 1089994at2759 |
PhylomeDBi | P67779 |
Family and domain databases
CDDi | cd03401, SPFH_prohibitin, 1 hit |
Gene3Di | 3.30.479.30, 1 hit |
InterProi | View protein in InterPro IPR001107, Band_7 IPR036013, Band_7/SPFH_dom_sf IPR000163, Prohibitin |
PANTHERi | PTHR23222, PTHR23222, 1 hit |
Pfami | View protein in Pfam PF01145, Band_7, 1 hit |
PRINTSi | PR00679, PROHIBITIN |
SMARTi | View protein in SMART SM00244, PHB, 1 hit |
SUPFAMi | SSF117892, SSF117892, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV
60 70 80 90 100
GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA
110 120 130 140 150
SQLPRIYTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV
160 170 180 190 200
SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV
210 220 230 240 250
EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ
260 270
LSRSRNITYL PAGQSVLLQL PQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M61219 mRNA Translation: AAA63500.1 BC072518 mRNA Translation: AAH72518.1 BC097304 mRNA Translation: AAH97304.1 |
PIRi | A39682 |
RefSeqi | NP_114039.1, NM_031851.2 |
Genome annotation databases
Ensembli | ENSRNOT00000075733; ENSRNOP00000066502; ENSRNOG00000046799 |
GeneIDi | 25344 |
KEGGi | rno:25344 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M61219 mRNA Translation: AAA63500.1 BC072518 mRNA Translation: AAH72518.1 BC097304 mRNA Translation: AAH97304.1 |
PIRi | A39682 |
RefSeqi | NP_114039.1, NM_031851.2 |
3D structure databases
SMRi | P67779 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247383, 5 interactors |
IntActi | P67779, 4 interactors |
MINTi | P67779 |
STRINGi | 10116.ENSRNOP00000066502 |
PTM databases
iPTMneti | P67779 |
PhosphoSitePlusi | P67779 |
2D gel databases
World-2DPAGEi | 0004:P67779 |
Proteomic databases
jPOSTi | P67779 |
PaxDbi | P67779 |
PRIDEi | P67779 |
Protocols and materials databases
DNASUi | 25344 |
Genome annotation databases
Ensembli | ENSRNOT00000075733; ENSRNOP00000066502; ENSRNOG00000046799 |
GeneIDi | 25344 |
KEGGi | rno:25344 |
Organism-specific databases
CTDi | 5245 |
RGDi | 3322, Phb |
Phylogenomic databases
eggNOGi | KOG3083, Eukaryota |
GeneTreei | ENSGT00950000183070 |
HOGENOMi | CLU_047969_0_0_1 |
InParanoidi | P67779 |
OMAi | RTRIRDN |
OrthoDBi | 1089994at2759 |
PhylomeDBi | P67779 |
Enzyme and pathway databases
Reactomei | R-RNO-5673000, RAF activation R-RNO-8949664, Processing of SMDT1 |
Miscellaneous databases
PROi | PR:P67779 |
Gene expression databases
Bgeei | ENSRNOG00000046799, Expressed in heart and 22 other tissues |
Genevisiblei | P67779, RN |
Family and domain databases
CDDi | cd03401, SPFH_prohibitin, 1 hit |
Gene3Di | 3.30.479.30, 1 hit |
InterProi | View protein in InterPro IPR001107, Band_7 IPR036013, Band_7/SPFH_dom_sf IPR000163, Prohibitin |
PANTHERi | PTHR23222, PTHR23222, 1 hit |
Pfami | View protein in Pfam PF01145, Band_7, 1 hit |
PRINTSi | PR00679, PROHIBITIN |
SMARTi | View protein in SMART SM00244, PHB, 1 hit |
SUPFAMi | SSF117892, SSF117892, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHB1_RAT | |
Accessioni | P67779Primary (citable) accession number: P67779 Secondary accession number(s): P24142, Q4V8M6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 23, 2022 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families