UniProtKB - P67701 (HIGA_ECOLI)
Protein
Antitoxin HigA
Gene
higA
Organism
Escherichia coli (strain K12)
Status
Functioni
Antitoxin component of a type II toxin-antitoxin (TA) system. Functions as an mRNA interferase antitoxin; overexpression prevents HigB-mediated cessation of cell growth and inhibition of cell proliferation.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 95 – 114 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
GO - Biological processi
- regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Toxin-antitoxin system, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:G7601-MONOMER MetaCyc:G7601-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Antitoxin HigA |
Gene namesi | Name:higA Synonyms:ygjM Ordered Locus Names:b3082, JW3053 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149763 | 1 – 138 | Antitoxin HigAAdd BLAST | 138 |
Proteomic databases
PaxDbi | P67701 |
PRIDEi | P67701 |
Expressioni
Inductioni
Induced by amino acid starvation and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HigA antitoxin. Transcription is negatively regulated by the cognate locus, probably by this protein. A member of the higB-higA operon.1 Publication
Interactioni
Subunit structurei
Probably forms a complex with the mRNA interferase HigB which inhibits the mRNA interferase activity.
CuratedBinary interactionsi
P67701
With | #Exp. | IntAct |
---|---|---|
higB [P64578] | 4 | EBI-1131548,EBI-1135071 |
Protein-protein interaction databases
BioGRIDi | 4262401, 7 interactors 851909, 3 interactors |
ComplexPortali | CPX-4121, HigAB toxin-antitoxin complex |
IntActi | P67701, 13 interactors |
STRINGi | 511145.b3082 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P67701 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 84 – 136 | HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST | 53 |
Sequence similaritiesi
Belongs to the HigA antitoxin family.Curated
Phylogenomic databases
eggNOGi | COG5499, Bacteria |
HOGENOMi | CLU_125852_0_0_6 |
Family and domain databases
CDDi | cd00093, HTH_XRE, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR039060, Antitox_HigA IPR001387, Cro/C1-type_HTH IPR010982, Lambda_DNA-bd_dom_sf |
PANTHERi | PTHR40455, PTHR40455, 1 hit |
Pfami | View protein in Pfam PF01381, HTH_3, 1 hit |
SMARTi | View protein in SMART SM00530, HTH_XRE, 1 hit |
SUPFAMi | SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS50943, HTH_CROC1, 1 hit |
i Sequence
Sequence statusi: Complete.
P67701-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIAIADILQA GEKLTAVAPF LAGIQNEEQY TQALELVDHL LLNDPENPLL
60 70 80 90 100
DLVCAKITAW EESAPEFAEF NAMAQAMPGG IAVIRTLMDQ YGLTLSDLPE
110 120 130
IGSKSMVSRV LSGKRKLTLE HAKKLATRFG ISPALFID
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57883.1 U00096 Genomic DNA Translation: AAC76117.1 AP009048 Genomic DNA Translation: BAE77132.1 |
PIRi | G65096 |
RefSeqi | NP_417553.1, NC_000913.3 WP_000560266.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76117; AAC76117; b3082 BAE77132; BAE77132; BAE77132 |
GeneIDi | 58462654 947593 |
KEGGi | ecj:JW3053 eco:b3082 |
PATRICi | fig|1411691.4.peg.3647 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57883.1 U00096 Genomic DNA Translation: AAC76117.1 AP009048 Genomic DNA Translation: BAE77132.1 |
PIRi | G65096 |
RefSeqi | NP_417553.1, NC_000913.3 WP_000560266.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IFG | X-ray | 2.70 | B/D | 1-138 | [»] | |
6JQ4 | X-ray | 2.00 | A | 1-138 | [»] | |
6KML | X-ray | 2.10 | B/D | 1-138 | [»] | |
6KMQ | X-ray | 2.35 | B | 1-138 | [»] | |
SMRi | P67701 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262401, 7 interactors 851909, 3 interactors |
ComplexPortali | CPX-4121, HigAB toxin-antitoxin complex |
IntActi | P67701, 13 interactors |
STRINGi | 511145.b3082 |
Proteomic databases
PaxDbi | P67701 |
PRIDEi | P67701 |
Genome annotation databases
EnsemblBacteriai | AAC76117; AAC76117; b3082 BAE77132; BAE77132; BAE77132 |
GeneIDi | 58462654 947593 |
KEGGi | ecj:JW3053 eco:b3082 |
PATRICi | fig|1411691.4.peg.3647 |
Organism-specific databases
EchoBASEi | EB2583 |
Phylogenomic databases
eggNOGi | COG5499, Bacteria |
HOGENOMi | CLU_125852_0_0_6 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7601-MONOMER MetaCyc:G7601-MONOMER |
Miscellaneous databases
PROi | PR:P67701 |
Family and domain databases
CDDi | cd00093, HTH_XRE, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR039060, Antitox_HigA IPR001387, Cro/C1-type_HTH IPR010982, Lambda_DNA-bd_dom_sf |
PANTHERi | PTHR40455, PTHR40455, 1 hit |
Pfami | View protein in Pfam PF01381, HTH_3, 1 hit |
SMARTi | View protein in SMART SM00530, HTH_XRE, 1 hit |
SUPFAMi | SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS50943, HTH_CROC1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIGA_ECOLI | |
Accessioni | P67701Primary (citable) accession number: P67701 Secondary accession number(s): P42594, Q2M9C4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families