UniProtKB - P67653 (KDSC_ECOL6)
Protein
3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC
Gene
kdsC
Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Functioni
Catalyzes the hydrolysis of 3-deoxy-D-manno-octulosonate 8-phosphate (KDO 8-P) to 3-deoxy-D-manno-octulosonate (KDO) and inorganic phosphate.1 Publication
Catalytic activityi
- 3-deoxy-α-D-manno-2-octulosonate-8-phosphate + H2O = 3-deoxy-α-D-manno-oct-2-ulosonate + phosphate1 PublicationEC:3.1.3.451 Publication
Cofactori
Mg2+1 Publication
: 3-deoxy-D-manno-octulosonate biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate.By similarityProteins known to be involved in the 3 steps of the subpathway in this organism are:
- Arabinose 5-phosphate isomerase KdsD (kdsD)
- 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA)
- 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC (kdsC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate, the pathway 3-deoxy-D-manno-octulosonate biosynthesis and in Carbohydrate biosynthesis.
Pathwayi: lipopolysaccharide biosynthesis
This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.By similarityView all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 32 | Magnesium1 Publication | 1 | |
Metal bindingi | 34 | Magnesium; via carbonyl oxygen1 Publication | 1 | |
Binding sitei | 34 | Substrate1 Publication | 1 | |
Binding sitei | 63 | Substrate1 Publication | 1 | |
Binding sitei | 78 | Substrate1 Publication | 1 | |
Binding sitei | 86 | Substrate1 Publication | 1 | |
Binding sitei | 102 | Substrate1 Publication | 1 | |
Metal bindingi | 125 | Magnesium1 Publication | 1 |
GO - Molecular functioni
- 3-deoxy-manno-octulosonate-8-phosphatase activity Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- lipopolysaccharide biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipopolysaccharide biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | ECOL199310:C3958-MONOMER |
UniPathwayi | UPA00030 UPA00357;UER00475 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsC (EC:3.1.3.451 Publication)Alternative name(s): KDO 8-P phosphatase |
Gene namesi | Name:kdsC Ordered Locus Names:c3958 |
Organismi | Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) |
Taxonomic identifieri | 199310 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201698 | 1 – 188 | 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase KdsCAdd BLAST | 188 |
Interactioni
Subunit structurei
Homotetramer.
1 PublicationProtein-protein interaction databases
STRINGi | 199310.c3958 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P67653 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P67653 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 55 – 59 | Substrate binding1 Publication | 5 |
Sequence similaritiesi
Belongs to the KdsC family.Curated
Phylogenomic databases
eggNOGi | COG1778, Bacteria |
HOGENOMi | CLU_106694_0_1_6 |
OMAi | FDENFHE |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR023214, HAD_sf IPR010023, KdsC_fam |
PIRSFi | PIRSF006118, KDO8-P_Ptase, 1 hit |
SFLDi | SFLDG01138, C1.6.2:_Deoxy-d-mannose-octulo, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01670, KdsC-phosphatas, 1 hit |
i Sequence
Sequence statusi: Complete.
P67653-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKAGASLAT CYGPVSADVM AKAENIRLLI LDVDGVLSDG LIYMGNNGEE
60 70 80 90 100
LKAFNVRDGY GIRCALTSDI EVAIITGRKA KLVEDRCATL GITHLYQGQS
110 120 130 140 150
NKLIAFSDLL EKLAIAPENV AYVGDDLIDW PVMEKVGLSV AVADAHPLLI
160 170 180
PRADYVTRIA GGRGAVREVC DLLLLAQGKL DEAKGQSI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014075 Genomic DNA Translation: AAN82398.1 |
RefSeqi | WP_000030016.1, NC_004431.1 |
Genome annotation databases
EnsemblBacteriai | AAN82398; AAN82398; c3958 |
KEGGi | ecc:c3958 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014075 Genomic DNA Translation: AAN82398.1 |
RefSeqi | WP_000030016.1, NC_004431.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2R8E | X-ray | 1.40 | A/B/C/D/E/F/G/H | 1-188 | [»] | |
2R8X | X-ray | 2.60 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P | 1-188 | [»] | |
2R8Y | X-ray | 1.85 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P | 1-188 | [»] | |
2R8Z | X-ray | 2.10 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P | 1-188 | [»] | |
3HYC | X-ray | 3.06 | A/B/C/D/E/F/G/H | 1-188 | [»] | |
3I6B | X-ray | 2.49 | A/B/C/D | 1-180 | [»] | |
SMRi | P67653 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 199310.c3958 |
Genome annotation databases
EnsemblBacteriai | AAN82398; AAN82398; c3958 |
KEGGi | ecc:c3958 |
Phylogenomic databases
eggNOGi | COG1778, Bacteria |
HOGENOMi | CLU_106694_0_1_6 |
OMAi | FDENFHE |
Enzyme and pathway databases
UniPathwayi | UPA00030 UPA00357;UER00475 |
BioCyci | ECOL199310:C3958-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P67653 |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR023214, HAD_sf IPR010023, KdsC_fam |
PIRSFi | PIRSF006118, KDO8-P_Ptase, 1 hit |
SFLDi | SFLDG01138, C1.6.2:_Deoxy-d-mannose-octulo, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01670, KdsC-phosphatas, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDSC_ECOL6 | |
Accessioni | P67653Primary (citable) accession number: P67653 Secondary accession number(s): Q83JF3, Q8FD72 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 95 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families