UniProtKB - P67612 (SYY_MYCBO)
Protein
Tyrosine--tRNA ligase
Gene
tyrS
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Functioni
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).UniRule annotation
Catalytic activityi
- EC:6.1.1.1UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 36 | TyrosineUniRule annotation | 1 | |
Binding sitei | 171 | TyrosineUniRule annotation | 1 | |
Binding sitei | 175 | TyrosineUniRule annotation | 1 | |
Binding sitei | 234 | ATPUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- RNA binding Source: UniProtKB-KW
- tyrosine-tRNA ligase activity Source: UniProtKB-UniRule
GO - Biological processi
- tyrosyl-tRNA aminoacylation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, RNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MBOV233413:G1GT4-1774-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine--tRNA ligaseUniRule annotation (EC:6.1.1.1UniRule annotation)Alternative name(s): Tyrosyl-tRNA synthetaseUniRule annotation Short name: TyrRSUniRule annotation |
Gene namesi | Name:tyrSUniRule annotation Ordered Locus Names:BQ2027_MB1715 |
Organismi | Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
Taxonomic identifieri | 233413 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055661 | 1 – 424 | Tyrosine--tRNA ligaseAdd BLAST | 424 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 356 – 413 | S4 RNA-bindingUniRule annotationAdd BLAST | 58 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 41 – 50 | 'HIGH' region | 10 | |
Motifi | 231 – 235 | 'KMSKS' region | 5 |
Sequence similaritiesi
Phylogenomic databases
OMAi | VNYMMAK |
Family and domain databases
CDDi | cd00165, S4, 1 hit cd00805, TyrRS_core, 1 hit |
Gene3Di | 3.10.290.10, 1 hit 3.40.50.620, 1 hit |
HAMAPi | MF_02006, Tyr_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002305, aa-tRNA-synth_Ic IPR014729, Rossmann-like_a/b/a_fold IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf IPR002307, Tyr-tRNA-ligase IPR024088, Tyr-tRNA-ligase_bac-type IPR024107, Tyr-tRNA-ligase_bac_1 |
PANTHERi | PTHR11766, PTHR11766, 1 hit |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit |
PRINTSi | PR01040, TRNASYNTHTYR |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
TIGRFAMsi | TIGR00234, tyrS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS50889, S4, 1 hit |
i Sequence
Sequence statusi: Complete.
P67612-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSGMILDELS WRGLIAQSTD LDTLAAEAQR GPMTVYAGFD PTAPSLHAGH
60 70 80 90 100
LVPLLTLRRF QRAGHRPIVL AGGATGMIGD PRDVGERSLN EADTVAEWTE
110 120 130 140 150
RIRGQLERFV DFDDSPMGAI VENNLEWTGS LSAIEFLRDI GKHFSVNVML
160 170 180 190 200
ARDTIRRRLA GEGISYTEFS YLLLQANDYV ELHRRHGCTL QIGGADQWGN
210 220 230 240 250
IIAGVRLVRQ KLGATVHALT VPLVTAADGT KFGKSTGGGS LWLDPQMTSP
260 270 280 290 300
YAWYQYFVNT ADADVIRYLR WFTFLSADEL AELEQATAQR PQQRAAQRRL
310 320 330 340 350
ASELTVLVHG EAATAAVEHA SRALFGRGEL ARLDEATLAA ALRETTVAEL
360 370 380 390 400
KPGSPDGIVD LLVASGLSAS KGAARRTIHE GGVSVNNIRV DNEEWVPQSS
410 420
DFLHGRWLVL RRGKRSIAGV ERIG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIU00319.1 |
RefSeqi | NP_855368.1, NC_002945.3 WP_003408375.1, NC_002945.4 |
Genome annotation databases
EnsemblBacteriai | SIU00319; SIU00319; BQ2027_MB1715 |
GeneIDi | 45425658 |
PATRICi | fig|233413.5.peg.1870 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIU00319.1 |
RefSeqi | NP_855368.1, NC_002945.3 WP_003408375.1, NC_002945.4 |
3D structure databases
SMRi | P67612 |
ModBasei | Search... |
Genome annotation databases
EnsemblBacteriai | SIU00319; SIU00319; BQ2027_MB1715 |
GeneIDi | 45425658 |
PATRICi | fig|233413.5.peg.1870 |
Phylogenomic databases
OMAi | VNYMMAK |
Enzyme and pathway databases
BioCyci | MBOV233413:G1GT4-1774-MONOMER |
Family and domain databases
CDDi | cd00165, S4, 1 hit cd00805, TyrRS_core, 1 hit |
Gene3Di | 3.10.290.10, 1 hit 3.40.50.620, 1 hit |
HAMAPi | MF_02006, Tyr_tRNA_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001412, aa-tRNA-synth_I_CS IPR002305, aa-tRNA-synth_Ic IPR014729, Rossmann-like_a/b/a_fold IPR002942, S4_RNA-bd IPR036986, S4_RNA-bd_sf IPR002307, Tyr-tRNA-ligase IPR024088, Tyr-tRNA-ligase_bac-type IPR024107, Tyr-tRNA-ligase_bac_1 |
PANTHERi | PTHR11766, PTHR11766, 1 hit |
Pfami | View protein in Pfam PF00579, tRNA-synt_1b, 1 hit |
PRINTSi | PR01040, TRNASYNTHTYR |
SMARTi | View protein in SMART SM00363, S4, 1 hit |
TIGRFAMsi | TIGR00234, tyrS, 1 hit |
PROSITEi | View protein in PROSITE PS00178, AA_TRNA_LIGASE_I, 1 hit PS50889, S4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SYY_MYCBO | |
Accessioni | P67612Primary (citable) accession number: P67612 Secondary accession number(s): A0A1R3XZ17, O33191, X2BII5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | April 7, 2021 | |
This is version 93 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - SIMILARITY comments
Index of protein domains and families