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UniProtKB - P67603 (AC4CH_ECOLI)

Entry version 113 (07 Apr 2021)
Sequence version 1 (11 Oct 2004)
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Protein

N(4)-acetylcytidine amidohydrolase

Gene

yqfB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the hydrolysis of N4-acetylcytidine (ac4C). Can also hydrolyze N4-acetyl-2'-deoxycytidine and N4-acetylcytosine with lower efficiency. Has weaker activity towards a wide range of structurally different N4-acylated cytosines and cytidines.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Unaffected by known inhibitors of amidohydrolases: PMSF, p-hydroxymercuribenzoate, p-chloromercuribenzoate and EDTA.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 157 sec(-1) with N4-acetylcytidine as substrate. kcat is 101 sec(-1) with N4-acetyl-2'deoxycytidine as substrate. kcat is 70 sec(-1) with N4-acetylcytosine as substrate. kcat is 24 sec(-1) with N4-hexanoyl-2'-deoxycytidine as substrate. kcat is 90 sec(-1) with N4-acetyl-5-fluorocytosine as substrate. kcat is 75 sec(-1) with N4-isobutyryl-2'-deoxycytidine as substrate. kcat is 1.8 sec(-1) with N4-benzoylcytidine as substrate. kcat is 0.07 sec(-1) with N4-benzoyl-2'-deoxycytidine as substrate.1 Publication
  1. KM=62 µM for N4-acetylcytidine1 Publication
  2. KM=400 µM for N4-acetyl-2'deoxycytidine1 Publication
  3. KM=70 µM for N4-acetylcytosine1 Publication
  4. KM=140 µM for N4-hexanoyl-2'-deoxycytidine1 Publication
  5. KM=2100 µM for N4-acetyl-5-fluorocytosine1 Publication
  6. KM=1900 µM for N4-isobutyryl-2'-deoxycytidine1 Publication
  7. KM=5100 µM for N4-benzoylcytidine1 Publication
  8. KM=300 µM for N4-benzoyl-2'-deoxycytidine1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    Temperature dependencei

    Optimum temperature is below 20 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei21Proton acceptorUniRule annotation1 Publication1
    Active sitei24NucleophileUniRule annotation1 Publication1
    Active sitei74Proton donorUniRule annotation1 Publication1

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    Complete GO annotation on QuickGO ...

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...    BioCyci    		EcoCyc:G7513-MONOMER
    MetaCyc:G7513-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    N(4)-acetylcytidine amidohydrolaseUniRule annotationCurated (EC:3.5.1.135UniRule annotation1 Publication)
    Short name:
    ac4C amidohydrolaseUniRule annotationCurated
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:yqfB
    Ordered Locus Names:b2900, JW2868
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    The mutant lacking the yqfB gene retains the ability to grow, albeit poorly, on N4-acetylcytosine as a source of uracil.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi21K → A: Loss of activity. 1 Publication1
    Mutagenesisi24T → A: Loss of activity. 1 Publication1
    Mutagenesisi26R → A: Loss of activity. 1 Publication1
    Mutagenesisi27D → A: 2-fold decrease in catalytic efficiency toward N(4)-acetylcytidine and 3-fold decrease in catalytic efficiency toward N(4)-acetylcytosine. 1 Publication1
    Mutagenesisi70H → A: 5-fold decrease in catalytic efficiency toward N(4)-acetylcytidine and 14-fold decrease in catalytic efficiency toward N(4)-acetylcytosine. 1 Publication1
    Mutagenesisi74E → A: 73-fold decrease in catalytic efficiency toward N(4)-acetylcytidine and 143-fold decrease in catalytic efficiency toward N(4)-acetylcytosine. 1 Publication1
    Mutagenesisi89Y → A: 115-fold decrease in catalytic efficiency toward N(4)-acetylcytidine and 312-fold decrease in catalytic efficiency toward N(4)-acetylcytosine. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002146001 – 103N(4)-acetylcytidine amidohydrolaseAdd BLAST103

    Proteomic databases

    jPOST - Japan Proteome Standard Repository/Database

    More...    jPOSTi    		P67603

    PaxDb, a database of protein abundance averages across all three domains of life

    More...    PaxDbi    		P67603

    PRoteomics IDEntifications database

    More...    PRIDEi    		P67603

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Monomer.

    1 Publication

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGRID)

    More...    BioGRIDi    		4263159, 125 interactors

    Protein interaction database and analysis system

    More...    IntActi    		P67603, 1 interactor

    STRING: functional protein association networks

    More...    STRINGi    		511145.b2900

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1103
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...    SMRi    		P67603

    Database of comparative protein structure models

    More...    ModBasei    		Search...

    Protein Data Bank in Europe - Knowledge Base

    More...    PDBe-KBi    		Search...

    Miscellaneous databases

    Relative evolutionary importance of amino acids within a protein sequence

    More...    EvolutionaryTracei    		P67603

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini6 – 101ASCHSequence analysisAdd BLAST96

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the N(4)-acetylcytidine amidohydrolase family.UniRule annotationCurated

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...    eggNOGi    		COG3097, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...    HOGENOMi    		CLU_152586_0_0_6

    InParanoid: Eukaryotic Ortholog Groups

    More...    InParanoidi    		P67603

    Family and domain databases

    HAMAP database of protein families

    More...    HAMAPi    		MF_00684, ac4C_amidohydr, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...    InterProi    		View protein in InterPro
    IPR008314, AC4CH
    IPR007374, ASCH_domain
    IPR015947, PUA-like_sf

    The PANTHER Classification System

    More...    PANTHERi    		PTHR38088, PTHR38088, 1 hit

    Pfam protein domain database

    More...    Pfami    		View protein in Pfam
    PF04266, ASCH, 1 hit

    PIRSF; a whole-protein classification database

    More...    PIRSFi    		PIRSF029143, UCP029143, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...    SMARTi    		View protein in SMART
    SM01022, ASCH, 1 hit

    Superfamily database of structural and functional annotation

    More...    SUPFAMi    		SSF88697, SSF88697, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P67603-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MQPNDITFFQ RFQDDILAGR KTITIRDESE SHFKTGDVLR VGRFEDDGYF 
            60         70         80         90        100
    CTIEVTATST VTLDTLTEKH AEQENMTLTE LKKVIADIYP GQTQFYVIEF 

    KCL
    Length:103
    Mass (Da):11,905
    Last modified:October 11, 2004 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBBC4E2AA87D6F2CE
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...    EMBLi

    GenBank nucleotide sequence database

    More...    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...    DDBJi
    Links Updated
    		U28375 Genomic DNA Translation: AAA83081.1
    U00096 Genomic DNA Translation: AAC75938.1
    AP009048 Genomic DNA Translation: BAE76965.1

    Protein sequence database of the Protein Information Resource

    More...    PIRi    		D65074

    NCBI Reference Sequences

    More...    RefSeqi    		NP_417376.1, NC_000913.3
    WP_001182957.1, NZ_SSUV01000019.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...    EnsemblBacteriai    		AAC75938; AAC75938; b2900
    BAE76965; BAE76965; BAE76965

    Database of genes from NCBI RefSeq genomes

    More...    GeneIDi    		56815490
    947380

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...    KEGGi    		ecj:JW2868
    eco:b2900

    Pathosystems Resource Integration Center (PATRIC)

    More...    PATRICi    		fig|511145.12.peg.2995

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		U28375 Genomic DNA Translation: AAA83081.1
    U00096 Genomic DNA Translation: AAC75938.1
    AP009048 Genomic DNA Translation: BAE76965.1
    PIRiD65074
    RefSeqiNP_417376.1, NC_000913.3
    WP_001182957.1, NZ_SSUV01000019.1

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...    PDBei

    Protein Data Bank RCSB

    More...    RCSB PDBi

    Protein Data Bank Japan

    More...    PDBji
    Links Updated
    		PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1TE7NMR-A1-103[»]
    SMRiP67603
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein-protein interaction databases

    BioGRIDi4263159, 125 interactors
    IntActiP67603, 1 interactor
    STRINGi511145.b2900

    Proteomic databases

    jPOSTiP67603
    PaxDbiP67603
    PRIDEiP67603

    Protocols and materials databases

    The DNASU plasmid repository

    More...    DNASUi    		947380

    Genome annotation databases

    EnsemblBacteriaiAAC75938; AAC75938; b2900
    BAE76965; BAE76965; BAE76965
    GeneIDi56815490
    947380
    KEGGiecj:JW2868
    eco:b2900
    PATRICifig|511145.12.peg.2995

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...    EchoBASEi    		EB2888

    Phylogenomic databases

    eggNOGiCOG3097, Bacteria
    HOGENOMiCLU_152586_0_0_6
    InParanoidiP67603

    Enzyme and pathway databases

    BioCyciEcoCyc:G7513-MONOMER
    MetaCyc:G7513-MONOMER

    Miscellaneous databases

    EvolutionaryTraceiP67603

    Protein Ontology

    More...    PROi    		PR:P67603

    Family and domain databases

    HAMAPiMF_00684, ac4C_amidohydr, 1 hit
    InterProiView protein in InterPro
    IPR008314, AC4CH
    IPR007374, ASCH_domain
    IPR015947, PUA-like_sf
    PANTHERiPTHR38088, PTHR38088, 1 hit
    PfamiView protein in Pfam
    PF04266, ASCH, 1 hit
    PIRSFiPIRSF029143, UCP029143, 1 hit
    SMARTiView protein in SMART
    SM01022, ASCH, 1 hit
    SUPFAMiSSF88697, SSF88697, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...    ProtoNeti    		Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...    MobiDBi    		Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAC4CH_ECOLI
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P67603
    Secondary accession number(s): Q2M9U1, Q46828
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: April 7, 2021
    This is version 113 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
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