UniProtKB - P67430 (NEMR_ECOLI)
Protein
HTH-type transcriptional repressor NemR
Gene
nemR
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in response to both electrophiles and reactive chlorine species (RCS) (PubMed:23506073, PubMed:23536188). Represses the transcription of the nemRA-gloA operon by binding to the NemR box (PubMed:18567656, PubMed:23506073, PubMed:23536188). May sense electrophiles, primarily quinones and glyoxals, as redox signals and regulate the redox state by modulating the expression of nemA and gloA (PubMed:23506073). Also uses the oxidation status of HOCl-sensitive cysteine residues to respond to bleach and related RCS (PubMed:23536188). Involved in response to methylglyoxal (PubMed:23646895).4 Publications
Activity regulationi
The redox state of the cysteines plays a crucial role in the regulation of NemR activity (PubMed:23506073, PubMed:23536188). Inactivated by reversible oxidation of cysteine residues in the presence of inducers such as quinones, glyoxals and reactive chlorine species (PubMed:23506073, PubMed:23536188). Also irreversibly inactivated by alkylation in the presence of N-ethylmaleimide (NEM) and other Cys modification reagents (PubMed:18567656). Inactivation by inducers decreases DNA-binding affinity and leads to the derepression of the nemRA-gloA operon (PubMed:18567656, PubMed:23506073, PubMed:23536188).3 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 106 | Plays a central role in response to RCS2 Publications | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 30 – 49 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-binding transcription factor activity Source: EcoCyc
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: EcoCyc
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Stress response, Transcription, Transcription regulation |
Enzyme and pathway databases
BioCyci | EcoCyc:G6889-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional repressor NemRCuratedAlternative name(s): Bleach-sensing transcription factor1 Publication Redox-regulated transcription factor NemRCurated |
Gene namesi | Name:nemR1 Publication Synonyms:ydhM Ordered Locus Names:b1649, JW5874 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Deletion mutant is more resistant to methylglyoxal than wild-type strain.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070639 | 1 – 199 | HTH-type transcriptional repressor NemRAdd BLAST | 199 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 21 | Interchain; in the presence of RES; transient1 Publication | ||
Disulfide bondi | 116 | Interchain; in the presence of RES; transient1 Publication |
Post-translational modificationi
Cys-21 and Cys-116 form reversible intermolecular disulfide bonds in the presence of reactive electrophilic species (RES). Formation of disulfide bonds leads to dimerization of NemR, which alters DNA binding affinity and NemR activity.1 Publication
Oxidized on Cys-106. Reversible oxidative modification of Cys-106 plays the central role in the sensing of and response to bleach and other RCS (PubMed:23536188). It leads to the formation of a reversible sulfenamide bond between Cys-106 and Lys-175 (PubMed:25867078). Oxidation alters DNA binding affinity and NemR activity (PubMed:23536188).2 Publications
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P67430 |
PaxDbi | P67430 |
PRIDEi | P67430 |
Expressioni
Inductioni
Interactioni
Binary interactionsi
P67430
With | #Exp. | IntAct |
---|---|---|
kdsB [P04951] | 3 | EBI-544803,EBI-544810 |
Protein-protein interaction databases
BioGRIDi | 4260266, 2 interactors 850526, 1 interactor |
DIPi | DIP-48165N |
IntActi | P67430, 1 interactor |
STRINGi | 511145.b1649 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P67430 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 67 | HTH tetR-typePROSITE-ProRule annotationAdd BLAST | 61 |
Phylogenomic databases
eggNOGi | COG1309, Bacteria |
HOGENOMi | CLU_069356_28_1_6 |
InParanoidi | P67430 |
PhylomeDBi | P67430 |
Family and domain databases
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001647, HTH_TetR IPR036271, Tet_transcr_reg_TetR-rel_C_sf IPR011075, TetR_C |
Pfami | View protein in Pfam PF16925, TetR_C_13, 1 hit PF00440, TetR_N, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF48498, SSF48498, 1 hit |
PROSITEi | View protein in PROSITE PS50977, HTH_TETR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P67430-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKHTEHDTR EHLLATGEQL CLQRGFTGMG LSELLKTAEV PKGSFYHYFR
60 70 80 90 100
SKEAFGVAML ERHYAAYHQR LTELLQSGEG NYRDRILAYY QQTLNQFCQH
110 120 130 140 150
GTISGCLTVK LSAEVCDLSE DMRSAMDKGA RGVIALLSQA LENGRENHCL
160 170 180 190
TFCGEPLQQA QVLYALWLGA NLQAKISRSF EPLENALAHV KNIIATPAV
Sequence cautioni
The sequence BAE76492 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74721.3 AP009048 Genomic DNA Translation: BAE76492.1 Different initiation. |
PIRi | C64922 |
RefSeqi | NP_416166.3, NC_000913.3 WP_001032936.1, NZ_STEB01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC74721; AAC74721; b1649 BAE76492; BAE76492; BAE76492 |
GeneIDi | 49584596 946166 |
KEGGi | ecj:JW5874 eco:b1649 |
PATRICi | fig|1411691.4.peg.610 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74721.3 AP009048 Genomic DNA Translation: BAE76492.1 Different initiation. |
PIRi | C64922 |
RefSeqi | NP_416166.3, NC_000913.3 WP_001032936.1, NZ_STEB01000003.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4YZE | X-ray | 2.20 | A/B/C/D | 1-199 | [»] | |
SMRi | P67430 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260266, 2 interactors 850526, 1 interactor |
DIPi | DIP-48165N |
IntActi | P67430, 1 interactor |
STRINGi | 511145.b1649 |
Proteomic databases
jPOSTi | P67430 |
PaxDbi | P67430 |
PRIDEi | P67430 |
Protocols and materials databases
DNASUi | 946166 |
Genome annotation databases
EnsemblBacteriai | AAC74721; AAC74721; b1649 BAE76492; BAE76492; BAE76492 |
GeneIDi | 49584596 946166 |
KEGGi | ecj:JW5874 eco:b1649 |
PATRICi | fig|1411691.4.peg.610 |
Organism-specific databases
EchoBASEi | EB3705 |
Phylogenomic databases
eggNOGi | COG1309, Bacteria |
HOGENOMi | CLU_069356_28_1_6 |
InParanoidi | P67430 |
PhylomeDBi | P67430 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6889-MONOMER |
Miscellaneous databases
PROi | PR:P67430 |
Family and domain databases
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR001647, HTH_TetR IPR036271, Tet_transcr_reg_TetR-rel_C_sf IPR011075, TetR_C |
Pfami | View protein in Pfam PF16925, TetR_C_13, 1 hit PF00440, TetR_N, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF48498, SSF48498, 1 hit |
PROSITEi | View protein in PROSITE PS50977, HTH_TETR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NEMR_ECOLI | |
Accessioni | P67430Primary (citable) accession number: P67430 Secondary accession number(s): P76189, Q2MB64 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references