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UniProtKB - P67087 (RSMI_ECOLI)
Protein
Ribosomal RNA small subunit methyltransferase I
Gene
rsmI
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the 2'-O-methylation of the ribose of cytidine 1402 (C1402) in 16S rRNA. In vitro, active on the assembled 30S subunit, but not naked 16S rRNA or 70S ribosomes.
1 PublicationCatalytic activityi
- cytidine1402 in 16S rRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine1402 in 16S rRNA + H+ + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.1981 Publication
GO - Molecular functioni
- rRNA (cytosine-2'-O-)-methyltransferase activity Source: EcoCyc
GO - Biological processi
- enzyme-directed rRNA 2'-O-methylation Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:G7641-MONOMER |
BRENDAi | 2.1.1.198, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase I (EC:2.1.1.198)Alternative name(s): 16S rRNA 2'-O-ribose C1402 methyltransferase rRNA (cytidine-2'-O-)-methyltransferase RsmI |
Gene namesi | Name:rsmI Synonyms:yraL Ordered Locus Names:b3146, JW3115 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000211938 | 1 – 286 | Ribosomal RNA small subunit methyltransferase IAdd BLAST | 286 |
Proteomic databases
jPOSTi | P67087 |
PaxDbi | P67087 |
PRIDEi | P67087 |
Interactioni
Binary interactionsi
P67087
With | #Exp. | IntAct |
---|---|---|
aceF [P06959] | 2 | EBI-561394,EBI-542707 |
Protein-protein interaction databases
BioGRIDi | 4261593, 91 interactors |
DIPi | DIP-12896N |
IntActi | P67087, 3 interactors |
STRINGi | 511145.b3146 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P67087 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0313, Bacteria |
HOGENOMi | CLU_044779_4_0_6 |
InParanoidi | P67087 |
PhylomeDBi | P67087 |
Family and domain databases
CDDi | cd11648, RsmI, 1 hit |
Gene3Di | 3.30.950.10, 1 hit 3.40.1010.10, 1 hit |
HAMAPi | MF_01877, 16SrRNA_methyltr_I, 1 hit |
InterProi | View protein in InterPro IPR000878, 4pyrrol_Mease IPR035996, 4pyrrol_Methylase_sf IPR014777, 4pyrrole_Mease_sub1 IPR014776, 4pyrrole_Mease_sub2 IPR008189, rRNA_ssu_MeTfrase_I IPR018063, SAM_MeTrfase_RsmI_CS |
PANTHERi | PTHR46111, PTHR46111, 1 hit |
Pfami | View protein in Pfam PF00590, TP_methylase, 1 hit |
PIRSFi | PIRSF005917, MTase_YraL, 1 hit |
SUPFAMi | SSF53790, SSF53790, 1 hit |
TIGRFAMsi | TIGR00096, TIGR00096, 1 hit |
PROSITEi | View protein in PROSITE PS01296, RSMI, 1 hit |
i Sequence
Sequence statusi: Complete.
P67087-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKQHQSADNS QGQLYIVPTP IGNLADITQR ALEVLQAVDL IAAEDTRHTG
60 70 80 90 100
LLLQHFGINA RLFALHDHNE QQKAETLLAK LQEGQNIALV SDAGTPLIND
110 120 130 140 150
PGYHLVRTCR EAGIRVVPLP GPCAAITALS AAGLPSDRFC YEGFLPAKSK
160 170 180 190 200
GRRDALKAIE AEPRTLIFYE STHRLLDSLE DIVAVLGESR YVVLARELTK
210 220 230 240 250
TWETIHGAPV GELLAWVKED ENRRKGEMVL IVEGHKAQEE DLPADALRTL
260 270 280
ALLQAELPLK KAAALAAEIH GVKKNALYKY ALEQQG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57949.1 U00096 Genomic DNA Translation: AAC76180.1 AP009048 Genomic DNA Translation: BAE77192.1 |
PIRi | F65104 |
RefSeqi | NP_417615.1, NC_000913.3 WP_000809262.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76180; AAC76180; b3146 BAE77192; BAE77192; BAE77192 |
GeneIDi | 58462813 947664 |
KEGGi | ecj:JW3115 eco:b3146 |
PATRICi | fig|1411691.4.peg.3584 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57949.1 U00096 Genomic DNA Translation: AAC76180.1 AP009048 Genomic DNA Translation: BAE77192.1 |
PIRi | F65104 |
RefSeqi | NP_417615.1, NC_000913.3 WP_000809262.1, NZ_STEB01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5HW4 | X-ray | 2.21 | A/B/C | 12-258 | [»] | |
SMRi | P67087 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261593, 91 interactors |
DIPi | DIP-12896N |
IntActi | P67087, 3 interactors |
STRINGi | 511145.b3146 |
Proteomic databases
jPOSTi | P67087 |
PaxDbi | P67087 |
PRIDEi | P67087 |
Genome annotation databases
EnsemblBacteriai | AAC76180; AAC76180; b3146 BAE77192; BAE77192; BAE77192 |
GeneIDi | 58462813 947664 |
KEGGi | ecj:JW3115 eco:b3146 |
PATRICi | fig|1411691.4.peg.3584 |
Organism-specific databases
EchoBASEi | EB2630 |
Phylogenomic databases
eggNOGi | COG0313, Bacteria |
HOGENOMi | CLU_044779_4_0_6 |
InParanoidi | P67087 |
PhylomeDBi | P67087 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7641-MONOMER |
BRENDAi | 2.1.1.198, 2026 |
Miscellaneous databases
PROi | PR:P67087 |
Family and domain databases
CDDi | cd11648, RsmI, 1 hit |
Gene3Di | 3.30.950.10, 1 hit 3.40.1010.10, 1 hit |
HAMAPi | MF_01877, 16SrRNA_methyltr_I, 1 hit |
InterProi | View protein in InterPro IPR000878, 4pyrrol_Mease IPR035996, 4pyrrol_Methylase_sf IPR014777, 4pyrrole_Mease_sub1 IPR014776, 4pyrrole_Mease_sub2 IPR008189, rRNA_ssu_MeTfrase_I IPR018063, SAM_MeTrfase_RsmI_CS |
PANTHERi | PTHR46111, PTHR46111, 1 hit |
Pfami | View protein in Pfam PF00590, TP_methylase, 1 hit |
PIRSFi | PIRSF005917, MTase_YraL, 1 hit |
SUPFAMi | SSF53790, SSF53790, 1 hit |
TIGRFAMsi | TIGR00096, TIGR00096, 1 hit |
PROSITEi | View protein in PROSITE PS01296, RSMI, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RSMI_ECOLI | |
Accessioni | P67087Primary (citable) accession number: P67087 Secondary accession number(s): P45528, Q2M964 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families