UniProtKB - P65689 (AHPE_MYCBO)
Protein
Alkyl hydroperoxide reductase E
Gene
ahpE
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. May represent an important antioxidant defense against cytotoxic peroxides, especially peroxynitrite, which can be formed by activated macrophages during infection.By similarity
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule. C(P) can be reactivated through a mixed disulfide with the N-terminal cysteine of mycoredoxin-1 (Mrx1), resolved by its C-terminal cysteine, or by a mixed disulfide with mycothiol, resolved by a second molecule of mycothiol or by mycoredoxin-1.By similarity
Catalytic activityi
- [mycoredoxin]-L-dithiol + a hydroperoxide = [mycoredoxin]-L-disulfide + an alcohol + H2OBy similarityEC:1.11.1.29By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 45 | By similarity | 1 |
GO - Molecular functioni
- peroxidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BioCyci | MBOV233413:G1GT4-2332-MONOMER |
Protein family/group databases
PeroxiBasei | 6017, MboAhpE |
Names & Taxonomyi
Protein namesi | Recommended name: Alkyl hydroperoxide reductase E (EC:1.11.1.29By similarity)Alternative name(s): Mycoredoxin-dependent peroxiredoxinCurated Peroxiredoxin AhpE Short name: Prx Thioredoxin peroxidase Short name: TPx |
Gene namesi | Name:ahpE Ordered Locus Names:BQ2027_MB2262C |
Organismi | Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
Taxonomic identifieri | 233413 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000135149 | 1 – 153 | Alkyl hydroperoxide reductase EAdd BLAST | 153 |
Interactioni
Subunit structurei
Homodimer. Forms both dimers and octamers; a tightly-associated dimer and a ring-like octamer.
By similarityFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 153 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 152 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
OMAi | FAFTGVC |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P65689-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNVGATAPD FTLRDQNQQL VTLRGYRGAK NVLLVFFPLA FTGICQGELD
60 70 80 90 100
QLRDHLPEFE NDDSAALAIS VGPPPTHKIW ATQSGFTFPL LSDFWPHGAV
110 120 130 140 150
SQAYGVFNEQ AGIANRGTFV VDRSGIIRFA EMKQPGEVRD QRLWTDALAA
LTA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIU00873.1 |
RefSeqi | NP_855911.1, NC_002945.3 WP_003411527.1, NC_002945.4 |
Genome annotation databases
EnsemblBacteriai | CDO43519; CDO43519; Mb2262c |
GeneIDi | 23491561 |
PATRICi | fig|233413.5.peg.2483 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIU00873.1 |
RefSeqi | NP_855911.1, NC_002945.3 WP_003411527.1, NC_002945.4 |
3D structure databases
SMRi | P65689 |
ModBasei | Search... |
Protein family/group databases
PeroxiBasei | 6017, MboAhpE |
Genome annotation databases
EnsemblBacteriai | CDO43519; CDO43519; Mb2262c |
GeneIDi | 23491561 |
PATRICi | fig|233413.5.peg.2483 |
Phylogenomic databases
OMAi | FAFTGVC |
Enzyme and pathway databases
BioCyci | MBOV233413:G1GT4-2332-MONOMER |
Family and domain databases
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000866, AhpC/TSA IPR024706, Peroxiredoxin_AhpC-typ IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF00578, AhpC-TSA, 1 hit |
PIRSFi | PIRSF000239, AHPC, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AHPE_MYCBO | |
Accessioni | P65689Primary (citable) accession number: P65689 Secondary accession number(s): A0A1R3Y0L1, Q10520, X2BK87 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | August 12, 2020 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |