UniProtKB - P64636 (YRFG_ECOLI)
Protein
GMP/IMP nucleotidase YrfG
Gene
yrfG
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the dephosphorylation of different purine nucleotides (GMP and IMP). Also hydrolyzes flavin mononucleotide (FMN).1 Publication
Catalytic activityi
- EC:3.1.3.5
Cofactori
Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Magnesium. Can also use other divalent metal cations as manganese, cobalt or zinc.1 Publication
Kineticsi
- KM=0.010 mM for IMP (with manganese ions as cofactor and at pH 9)1 Publication
- KM=0.012 mM for GMP (with manganese ions as cofactor and at pH 9)1 Publication
- KM=0.062 mM for GMP (with magnesium ions as cofactor and at pH 9)1 Publication
- KM=0.22 mM for IMP (with magnesium ions as cofactor and at pH 9)1 Publication
pH dependencei
Optimum pH is between 6 and 7.5.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 9 | NucleophileBy similarity | 1 | |
Metal bindingi | 9 | MagnesiumBy similarity | 1 | |
Metal bindingi | 11 | MagnesiumBy similarity | 1 | |
Binding sitei | 149 | SubstrateBy similarity | 1 | |
Metal bindingi | 174 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- GMP 5'-nucleotidase activity Source: EcoCyc
- IMP 5'-nucleotidase activity Source: EcoCyc
- magnesium ion binding Source: UniProtKB
- manganese ion binding Source: UniProtKB
- phosphoglycolate phosphatase activity Source: GO_Central
- purine nucleosidase activity Source: EcoliWiki
GO - Biological processi
- dephosphorylation Source: EcoliWiki
- DNA repair Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7742-MONOMER MetaCyc:G7742-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yrfG Ordered Locus Names:b3399, JW5865 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169542 | 1 – 222 | GMP/IMP nucleotidase YrfGAdd BLAST | 222 |
Proteomic databases
PaxDbi | P64636 |
PRIDEi | P64636 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260978, 19 interactors |
STRINGi | 511145.b3399 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 9 – 11 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the HAD-like hydrolase superfamily.Curated
Phylogenomic databases
eggNOGi | COG1011, Bacteria |
HOGENOMi | CLU_106706_0_0_6 |
InParanoidi | P64636 |
PhylomeDBi | P64636 |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006439, HAD-SF_hydro_IA IPR041492, HAD_2 IPR023214, HAD_sf |
Pfami | View protein in Pfam PF13419, HAD_2, 1 hit |
PRINTSi | PR00413, HADHALOGNASE |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01509, HAD-SF-IA-v3, 1 hit |
i Sequence
Sequence statusi: Complete.
P64636-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHINIAWQDV DTVLLDMDGT LLDLAFDNYF WQKLVPETWG AKNGVTPQEA
60 70 80 90 100
MEYMRQQYHD VQHTLNWYCL DYWSEQLGLD ICAMTTEMGP RAVLREDTIP
110 120 130 140 150
FLEALKASGK QRILLTNAHP HNLAVKLEHT GLDAHLDLLL STHTFGYPKE
160 170 180 190 200
DQRLWHAVAE ATGLKAERTL FIDDSEAILD AAAQFGIRYC LGVTNPDSGI
210 220
AEKQYQRHPS LNDYRRLIPS LM
Sequence cautioni
The sequence AAA58196 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58196.1 Different initiation. U00096 Genomic DNA Translation: AAC76424.2 AP009048 Genomic DNA Translation: BAE77892.1 |
PIRi | B65135 |
RefSeqi | NP_417858.4, NC_000913.3 WP_001295168.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76424; AAC76424; b3399 BAE77892; BAE77892; BAE77892 |
GeneIDi | 58388120 947904 |
KEGGi | ecj:JW5865 eco:b3399 |
PATRICi | fig|1411691.4.peg.3331 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58196.1 Different initiation. U00096 Genomic DNA Translation: AAC76424.2 AP009048 Genomic DNA Translation: BAE77892.1 |
PIRi | B65135 |
RefSeqi | NP_417858.4, NC_000913.3 WP_001295168.1, NZ_STEB01000004.1 |
3D structure databases
SMRi | P64636 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260978, 19 interactors |
STRINGi | 511145.b3399 |
Proteomic databases
PaxDbi | P64636 |
PRIDEi | P64636 |
Genome annotation databases
EnsemblBacteriai | AAC76424; AAC76424; b3399 BAE77892; BAE77892; BAE77892 |
GeneIDi | 58388120 947904 |
KEGGi | ecj:JW5865 eco:b3399 |
PATRICi | fig|1411691.4.peg.3331 |
Organism-specific databases
EchoBASEi | EB2764 |
Phylogenomic databases
eggNOGi | COG1011, Bacteria |
HOGENOMi | CLU_106706_0_0_6 |
InParanoidi | P64636 |
PhylomeDBi | P64636 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7742-MONOMER MetaCyc:G7742-MONOMER |
Miscellaneous databases
PROi | PR:P64636 |
Family and domain databases
Gene3Di | 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR036412, HAD-like_sf IPR006439, HAD-SF_hydro_IA IPR041492, HAD_2 IPR023214, HAD_sf |
Pfami | View protein in Pfam PF13419, HAD_2, 1 hit |
PRINTSi | PR00413, HADHALOGNASE |
SUPFAMi | SSF56784, SSF56784, 1 hit |
TIGRFAMsi | TIGR01509, HAD-SF-IA-v3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YRFG_ECOLI | |
Accessioni | P64636Primary (citable) accession number: P64636 Secondary accession number(s): P45801, Q2M764 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 113 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families