UniProtKB - P64612 (ZAPE_ECOLI)
Protein
Cell division protein ZapE
Gene
zapE
Organism
Escherichia coli (strain K12)
Status
Functioni
Reduces the stability of FtsZ polymers in the presence of ATP. Required for cell division under low-oxygen conditions. Hydrolyzes ATP but not GTP.UniRule annotation1 Publication
Miscellaneous
ZapE abundance has to be tightly regulated to allow cell division. Loss or overexpression of ZapE alters Z-ring stability and leads to bacterial filamentation (PubMed:24595368).1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 78 – 85 | ATPCurated | 8 |
GO - Molecular functioni
- ATPase activity Source: EcoCyc
- ATP binding Source: UniProtKB-UniRule
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Biological process | Cell cycle, Cell division |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G7680-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Cell division protein ZapEUniRule annotationAlternative name(s): Z ring-associated protein ZapEUniRule annotation |
Gene namesi | Name:zapEUniRule annotation Synonyms:yhcM Ordered Locus Names:b3232, JW3201 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation1 Publication
Note: Localizes to the constricting Z-ring during late stages of the cell division process.
Other locations
- cell division site Source: EcoCyc
- cytoplasm Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Mutants have no growth defect in aerobic conditions. However, inactivation leads to a stress-dependent elongated phenotype (in anaerobiosis or at temperatures over 37 degrees Celsius).1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 84 | K → A: Abolishes ATPase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169487 | 1 – 375 | Cell division protein ZapEAdd BLAST | 375 |
Proteomic databases
jPOSTi | P64612 |
PaxDbi | P64612 |
PRIDEi | P64612 |
Interactioni
Subunit structurei
Interacts with FtsZ.
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4261913, 23 interactors |
DIPi | DIP-48198N |
IntActi | P64612, 1 interactor |
STRINGi | 511145.b3232 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1485, Bacteria |
HOGENOMi | CLU_008681_0_4_6 |
InParanoidi | P64612 |
PhylomeDBi | P64612 |
Family and domain databases
HAMAPi | MF_01919, ZapE, 1 hit |
InterProi | View protein in InterPro IPR005654, ATPase_AFG1-like IPR027417, P-loop_NTPase IPR030870, ZapE |
PANTHERi | PTHR12169, PTHR12169, 1 hit |
Pfami | View protein in Pfam PF03969, AFG1_ATPase, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
i Sequence
Sequence statusi: Complete.
P64612-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQSVTPTSQY LKALNEGSHQ PDDVQKEAVS RLEIIYQELI NSTPPAPRTS
60 70 80 90 100
GLMARVGKLW GKREDTKHTP VRGLYMWGGV GRGKTWLMDL FYQSLPGERK
110 120 130 140 150
QRLHFHRFML RVHEELTALQ GQTDPLEIIA DRFKAETDVL CFDEFFVSDI
160 170 180 190 200
TDAMLLGGLM KALFARGITL VATSNIPPDE LYRNGLQRAR FLPAIDAIKQ
210 220 230 240 250
HCDVMNVDAG VDYRLRTLTQ AHLWLSPLHD ETRAQMDKLW LALAGGKREN
260 270 280 290 300
SPTLEINHRP LATMGVENQT LAVSFTTLCV DARSQHDYIA LSRLFHTVML
310 320 330 340 350
FDVPVMTRLM ESEARRFIAL VDEFYERHVK LVVSAEVPLY EIYQGDRLKF
360 370
EFQRCLSRLQ EMQSEEYLKR EHLAG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58034.1 U00096 Genomic DNA Translation: AAC76264.1 AP009048 Genomic DNA Translation: BAE77275.1 |
PIRi | B65115 |
RefSeqi | NP_417699.1, NC_000913.3 WP_001192332.1, NZ_SSZK01000034.1 |
Genome annotation databases
EnsemblBacteriai | AAC76264; AAC76264; b3232 BAE77275; BAE77275; BAE77275 |
GeneIDi | 57728497 947821 |
KEGGi | ecj:JW3201 eco:b3232 |
PATRICi | fig|511145.12.peg.3329 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA58034.1 U00096 Genomic DNA Translation: AAC76264.1 AP009048 Genomic DNA Translation: BAE77275.1 |
PIRi | B65115 |
RefSeqi | NP_417699.1, NC_000913.3 WP_001192332.1, NZ_SSZK01000034.1 |
3D structure databases
SMRi | P64612 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261913, 23 interactors |
DIPi | DIP-48198N |
IntActi | P64612, 1 interactor |
STRINGi | 511145.b3232 |
Proteomic databases
jPOSTi | P64612 |
PaxDbi | P64612 |
PRIDEi | P64612 |
Genome annotation databases
EnsemblBacteriai | AAC76264; AAC76264; b3232 BAE77275; BAE77275; BAE77275 |
GeneIDi | 57728497 947821 |
KEGGi | ecj:JW3201 eco:b3232 |
PATRICi | fig|511145.12.peg.3329 |
Organism-specific databases
EchoBASEi | EB2670 |
Phylogenomic databases
eggNOGi | COG1485, Bacteria |
HOGENOMi | CLU_008681_0_4_6 |
InParanoidi | P64612 |
PhylomeDBi | P64612 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7680-MONOMER |
Miscellaneous databases
PROi | PR:P64612 |
Family and domain databases
HAMAPi | MF_01919, ZapE, 1 hit |
InterProi | View protein in InterPro IPR005654, ATPase_AFG1-like IPR027417, P-loop_NTPase IPR030870, ZapE |
PANTHERi | PTHR12169, PTHR12169, 1 hit |
Pfami | View protein in Pfam PF03969, AFG1_ATPase, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ZAPE_ECOLI | |
Accessioni | P64612Primary (citable) accession number: P64612 Secondary accession number(s): P46442, Q2M8Y1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families