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Protein

Intermembrane phospholipid transport system binding protein MlaB

Gene

mlaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MlaFEDB, which is involved in a phospholipid transport pathway that maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane (PubMed:19383799, PubMed:27529189). MlaB plays critical roles in both the assembly and activity of the complex. May act by modulating MlaF structure and stability (PubMed:27529189).2 Publications

GO - Molecular functioni

  • ATPase activity Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoCyc
  • response to antibiotic Source: EcoCyc

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:G7658-MONOMER

Protein family/group databases

TCDBi3.A.1.27.3 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Intermembrane phospholipid transport system binding protein MlaBCurated
Gene namesi
Name:mlaB1 Publication
Synonyms:yrbB
Ordered Locus Names:b3191, JW5535
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12797 mlaB

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutation leads to accumulation of phospholipid in the outer leaflet of the outer membrane and increased outer membrane permeability. It confers sensitivity to SDS-EDTA.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52T → A: Does not affect the assembly of the MlaFEB complex, but abolishes ATPase activity. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL3309025

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001694621 – 97Intermembrane phospholipid transport system binding protein MlaBAdd BLAST97

Proteomic databases

PaxDbiP64602
PRIDEiP64602

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MlaF), two transmembrane proteins (MlaE), two cytoplasmic solute-binding proteins (MlaB) and six periplamic solute-binding proteins (MlaD).1 Publication2 Publications

Protein-protein interaction databases

BioGridi4260758, 427 interactors
DIPiDIP-47898N
IntActiP64602, 3 interactors
STRINGi316385.ECDH10B_3365

Structurei

3D structure databases

ProteinModelPortaliP64602
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 97STASPROSITE-ProRule annotationAdd BLAST97

Phylogenomic databases

eggNOGiENOG4105UE2 Bacteria
COG3113 LUCA
HOGENOMiHOG000287451
KOiK07122
OMAiNPLWDAR

Family and domain databases

Gene3Di3.30.750.24, 1 hit
InterProiView protein in InterPro
IPR002645 STAS_dom
IPR036513 STAS_dom_sf
PfamiView protein in Pfam
PF13466 STAS_2, 1 hit
SUPFAMiSSF52091 SSF52091, 1 hit
PROSITEiView protein in PROSITE
PS50801 STAS, 1 hit

Sequencei

Sequence statusi: Complete.

P64602-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESLSWMQT GDTLALSGEL DQDVLLPLWE MREEAVKGIT CIDLSRVSRV
60 70 80 90
DTGGLALLLH LIDLAKKQGN NVTLQGVNDK VYTLAKLYNL PADVLPR
Length:97
Mass (Da):10,680
Last modified:October 11, 2004 - v1
Checksum:iBB71D8F0A23D0225
GO

Sequence cautioni

The sequence AAA57992 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57992.1 Different initiation.
U00096 Genomic DNA Translation: AAC76223.2
AP009048 Genomic DNA Translation: BAE77235.1
RefSeqiNP_417658.4, NC_000913.3
WP_000004488.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76223; AAC76223; b3191
BAE77235; BAE77235; BAE77235
GeneIDi947954
KEGGiecj:JW5535
eco:b3191
PATRICifig|1411691.4.peg.3540

Similar proteinsi

Entry informationi

Entry nameiMLAB_ECOLI
AccessioniPrimary (citable) accession number: P64602
Secondary accession number(s): P45389, Q2M921
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 18, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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