UniProtKB - P64554 (QUEE_ECOLI)
Protein
7-carboxy-7-deazaguanine synthase
Gene
queE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.UniRule annotation
Catalytic activityi
- EC:4.3.99.3UniRule annotation
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
- S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation
- Mg2+UniRule annotation
: 7-cyano-7-deazaguanine biosynthesis Pathwayi
This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 27 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 31 | Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 35 | Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 38 | Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation | 1 | |
Metal bindingi | 40 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 92 | SubstrateUniRule annotation | 1 | |
Binding sitei | 94 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-UniRule
- carbon-nitrogen lyase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- S-adenosyl-L-methionine binding Source: UniProtKB-UniRule
GO - Biological processi
- queuosine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Queuosine biosynthesis |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:G7443-MONOMER ECOL316407:JW2748-MONOMER |
UniPathwayi | UPA00391 |
Names & Taxonomyi
Protein namesi | Recommended name: 7-carboxy-7-deazaguanine synthaseUniRule annotation (EC:4.3.99.3UniRule annotation)Short name: CDG synthaseUniRule annotation Alternative name(s): Queuosine biosynthesis protein QueEUniRule annotation |
Gene namesi | Name:queEUniRule annotation Synonyms:ygcF Ordered Locus Names:b2777, JW2748 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169316 | 1 – 223 | 7-carboxy-7-deazaguanine synthaseAdd BLAST | 223 |
Proteomic databases
jPOSTi | P64554 |
PaxDbi | P64554 |
PRIDEi | P64554 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
BioGridi | 4262295, 24 interactors |
DIPi | DIP-48192N |
IntActi | P64554, 3 interactors |
STRINGi | 511145.b2777 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 12 – 14 | Substrate bindingUniRule annotation | 3 | |
Regioni | 136 – 138 | S-adenosyl-L-methionine bindingUniRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105EIZ Bacteria COG0602 LUCA |
HOGENOMi | HOG000266143 |
InParanoidi | P64554 |
KOi | K10026 |
PhylomeDBi | P64554 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00917 QueE, 1 hit |
InterProi | View protein in InterPro IPR024924 7-CO-7-deazaguanine_synth-like IPR013785 Aldolase_TIM IPR007197 rSAM IPR027609 rSAM_QueE_Proteobac |
Pfami | View protein in Pfam PF04055 Radical_SAM, 1 hit |
PIRSFi | PIRSF000370 QueE, 1 hit |
SFLDi | SFLDS00029 Radical_SAM, 1 hit |
TIGRFAMsi | TIGR04322 rSAM_QueE_Ecoli, 1 hit |
i Sequence
Sequence statusi: Complete.
P64554-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQYPINEMFQ TLQGEGYFTG VPAIFIRLQG CPVGCAWCDT KHTWEKLEDR
60 70 80 90 100
EVSLFSILAK TKESDKWGAA SSEDLLAVIG RQGYTARHVV ITGGEPCIHD
110 120 130 140 150
LLPLTDLLEK NGFSCQIETS GTHEVRCTPN TWVTVSPKLN MRGGYEVLSQ
160 170 180 190 200
ALERANEIKH PVGRVRDIEA LDELLATLTD DKPRVIALQP ISQKDDATRL
210 220
CIETCIARNW RLSMQTHKYL NIA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29580 Genomic DNA Translation: AAA69287.1 U00096 Genomic DNA Translation: AAC75819.1 AP009048 Genomic DNA Translation: BAE76851.1 |
PIRi | E65059 |
RefSeqi | NP_417257.1, NC_000913.3 WP_001199973.1, NZ_SSZK01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC75819; AAC75819; b2777 BAE76851; BAE76851; BAE76851 |
GeneIDi | 947527 |
KEGGi | ecj:JW2748 eco:b2777 |
PATRICi | fig|1411691.4.peg.3959 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U29580 Genomic DNA Translation: AAA69287.1 U00096 Genomic DNA Translation: AAC75819.1 AP009048 Genomic DNA Translation: BAE76851.1 |
PIRi | E65059 |
RefSeqi | NP_417257.1, NC_000913.3 WP_001199973.1, NZ_SSZK01000003.1 |
3D structure databases
SMRi | P64554 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 4262295, 24 interactors |
DIPi | DIP-48192N |
IntActi | P64554, 3 interactors |
STRINGi | 511145.b2777 |
Proteomic databases
jPOSTi | P64554 |
PaxDbi | P64554 |
PRIDEi | P64554 |
Genome annotation databases
EnsemblBacteriai | AAC75819; AAC75819; b2777 BAE76851; BAE76851; BAE76851 |
GeneIDi | 947527 |
KEGGi | ecj:JW2748 eco:b2777 |
PATRICi | fig|1411691.4.peg.3959 |
Organism-specific databases
EchoBASEi | EB2849 |
Phylogenomic databases
eggNOGi | ENOG4105EIZ Bacteria COG0602 LUCA |
HOGENOMi | HOG000266143 |
InParanoidi | P64554 |
KOi | K10026 |
PhylomeDBi | P64554 |
Enzyme and pathway databases
UniPathwayi | UPA00391 |
BioCyci | EcoCyc:G7443-MONOMER ECOL316407:JW2748-MONOMER |
Miscellaneous databases
PROi | PR:P64554 |
Family and domain databases
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00917 QueE, 1 hit |
InterProi | View protein in InterPro IPR024924 7-CO-7-deazaguanine_synth-like IPR013785 Aldolase_TIM IPR007197 rSAM IPR027609 rSAM_QueE_Proteobac |
Pfami | View protein in Pfam PF04055 Radical_SAM, 1 hit |
PIRSFi | PIRSF000370 QueE, 1 hit |
SFLDi | SFLDS00029 Radical_SAM, 1 hit |
TIGRFAMsi | TIGR04322 rSAM_QueE_Ecoli, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | QUEE_ECOLI | |
Accessioni | P64554Primary (citable) accession number: P64554 Secondary accession number(s): P55139, Q2MA55 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | November 13, 2019 | |
This is version 104 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene - PATHWAY comments
Index of metabolic and biosynthesis pathways