UniProtKB - P64248 (ASNH_MYCBO)
Protein
Putative asparagine synthetase [glutamine-hydrolyzing]
Gene
asnB
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Functioni
Catalytic activityi
- EC:6.3.5.4
: L-asparagine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).Proteins known to be involved in this subpathway in this organism are:
- Putative asparagine synthetase [glutamine-hydrolyzing] (asnB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | For GATase activityBy similarity | 1 | |
Binding sitei | 115 | GlutamineBy similarity | 1 | |
Sitei | 384 | Important for beta-aspartyl-AMP intermediate formationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 382 – 383 | ATPBy similarity | 2 |
GO - Molecular functioni
- asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
GO - Biological processi
- glutamine metabolic process Source: UniProtKB-KW
- L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Ligase |
Biological process | Amino-acid biosynthesis, Asparagine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MBOV233413:G1GT4-2292-MONOMER |
UniPathwayi | UPA00134;UER00195 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:asnB Ordered Locus Names:BQ2027_MB2224 |
Organismi | Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) |
Taxonomic identifieri | 233413 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000056936 | 2 – 652 | Putative asparagine synthetase [glutamine-hydrolyzing]Add BLAST | 651 |
Expressioni
Inductioni
Induced in response to the thiol oxidant diamide.1 Publication
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 231 | Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST | 230 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 60 – 64 | Glutamine bindingBy similarity | 5 | |
Regioni | 89 – 91 | Glutamine bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the asparagine synthetase family.Curated
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
OMAi | IEHSHQP |
Family and domain databases
CDDi | cd01991, Asn_Synthase_B_C, 1 hit cd00712, AsnB, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR006426, Asn_synth_AEB IPR001962, Asn_synthase IPR033738, AsnB_N IPR017932, GATase_2_dom IPR029055, Ntn_hydrolases_N |
Pfami | View protein in Pfam PF00733, Asn_synthase, 1 hit PF13537, GATase_7, 1 hit |
PIRSFi | PIRSF001589, Asn_synthetase_glu-h, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01536, asn_synth_AEB, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P64248-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MCGLLAFVAA PAGAAGPEGA DAASAIARAS HLMRHRGPDE SGTWHAVDGA
60 70 80 90 100
SGGVVFGFNR LSIIDIAHSH QPLRWGPPEA PDRYVLVFNG EIYNYLELRD
110 120 130 140 150
ELRTQHGAVF ATDGDGEAIL AGYHHWGTEV LQRLRGMFAF ALWDTVTREL
160 170 180 190 200
FCARDPFGIK PLFIATGAGG TAVASEKKCL LDLVELVGFD TEIDHRALQH
210 220 230 240 250
YTVLQYVPEP ETLHRGVRRL ESGCFARIRA DQLAPVITRY FVPRFAASPI
260 270 280 290 300
TNDNDQARYD EITAVLEDSV AKHMRADVTV GAFLSGGIDS TAIAALAIRH
310 320 330 340 350
NPRLITFTTG FEREGFSEID VAVASAEAIG ARHIAKVVSA DEFVAALPEI
360 370 380 390 400
VWYLDEPVAD PALVPLFFVA REARKHVKVV LSGEGADELF GGYTIYREPL
410 420 430 440 450
SLRPFDYLPK PLRRSMGKVS KPLPEGMRGK SLLHRGSLTL EERYYGNARS
460 470 480 490 500
FSGAQLREVL PGFRPDWTHT DVTAPVYAES AGWDPVARMQ HIDLFTWLRG
510 520 530 540 550
DILVKADKIT MANSLELRVP FLDPEVFAVA SRLPAGAKIT RTTTKYALRR
560 570 580 590 600
ALEPIVPAHV LHRPKLGFPV PIRHWLRAGE LLEWAYATVG SSQAGHLVDI
610 620 630 640 650
AAVYRMLDEH RCGSSDHSRR LWTMLIFMLW HAIFVEHSVV PQISEPQYPV
QL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIU00832.1 |
RefSeqi | NP_855873.1, NC_002945.3 WP_003411413.1, NC_002945.4 |
Genome annotation databases
EnsemblBacteriai | SIU00832; SIU00832; BQ2027_MB2224 |
PATRICi | fig|233413.5.peg.2440 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | LT708304 Genomic DNA Translation: SIU00832.1 |
RefSeqi | NP_855873.1, NC_002945.3 WP_003411413.1, NC_002945.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
EnsemblBacteriai | SIU00832; SIU00832; BQ2027_MB2224 |
PATRICi | fig|233413.5.peg.2440 |
Phylogenomic databases
OMAi | IEHSHQP |
Enzyme and pathway databases
UniPathwayi | UPA00134;UER00195 |
BioCyci | MBOV233413:G1GT4-2292-MONOMER |
Family and domain databases
CDDi | cd01991, Asn_Synthase_B_C, 1 hit cd00712, AsnB, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR006426, Asn_synth_AEB IPR001962, Asn_synthase IPR033738, AsnB_N IPR017932, GATase_2_dom IPR029055, Ntn_hydrolases_N |
Pfami | View protein in Pfam PF00733, Asn_synthase, 1 hit PF13537, GATase_7, 1 hit |
PIRSFi | PIRSF001589, Asn_synthetase_glu-h, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR01536, asn_synth_AEB, 1 hit |
PROSITEi | View protein in PROSITE PS51278, GATASE_TYPE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASNH_MYCBO | |
Accessioni | P64248Primary (citable) accession number: P64248 Secondary accession number(s): A0A1R3Y0J6, Q10374, X2BKF1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | April 7, 2021 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families