UniProtKB - P63488 (GATA_STAAM)
Protein
Glutamyl-tRNA(Gln) amidotransferase subunit A
Gene
gatA
Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Functioni
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation
Catalytic activityi
- ATP + H2O + L-glutamine + L-glutamyl-tRNAGln = ADP + H+ + L-glutamate + L-glutaminyl-tRNAGln + phosphateUniRule annotationEC:6.3.5.7UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 79 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 154 | Charge relay systemUniRule annotation | 1 | |
Active sitei | 178 | Acyl-ester intermediateUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: UniProtKB-UniRule
- hydrolase activity Source: InterPro
GO - Biological processi
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | SAUR158878:SAV_RS10410-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)Short name: Glu-ADT subunit AUniRule annotation |
Gene namesi | Name:gatAUniRule annotation Ordered Locus Names:SAV1900 |
Organismi | Staphylococcus aureus (strain Mu50 / ATCC 700699) |
Taxonomic identifieri | 158878 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- glutamyl-tRNA(Gln) amidotransferase complex Source: InterPro
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105199 | 1 – 485 | Glutamyl-tRNA(Gln) amidotransferase subunit AAdd BLAST | 485 |
Proteomic databases
PaxDbi | P63488 |
2D gel databases
World-2DPAGEi | 0002:P63488 |
Interactioni
Subunit structurei
Heterotrimer of A, B and C subunits.
UniRule annotationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P63488 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P63488 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
HOGENOMi | CLU_009600_0_3_9 |
OMAi | MYLSDIF |
PhylomeDBi | P63488 |
Family and domain databases
Gene3Di | 3.90.1300.10, 1 hit |
HAMAPi | MF_00120, GatA, 1 hit |
InterProi | View protein in InterPro IPR000120, Amidase IPR020556, Amidase_CS IPR023631, Amidase_dom IPR036928, AS_sf IPR004412, GatA |
PANTHERi | PTHR11895, PTHR11895, 1 hit |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit |
SUPFAMi | SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR00132, gatA, 1 hit |
PROSITEi | View protein in PROSITE PS00571, AMIDASES, 1 hit |
i Sequence
Sequence statusi: Complete.
P63488-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSIRYESVEN LLTLIKDKKI KPSDVVKDIY DAIEETDPTI KSFLALDKEN
60 70 80 90 100
AIKKAQELDE LQAKDQMDGK LFGIPMGIKD NIITNGLETT CASKMLEGFV
110 120 130 140 150
PIYESTVMEK LHKENAVLIG KLNMDEFAMG GSTETSYFKK TVNPFDHKAV
160 170 180 190 200
PGGSSGGSAA AVAAGLVPLS LGSDTGGSIR QPAAYCGVVG MKPTYGRVSR
210 220 230 240 250
FGLVAFASSL DQIGPLTRNV KDNAIVLEAI SGADVNDSTS APVDDVDFTS
260 270 280 290 300
EIGKDIKGLK VALPKEYLGE GVADDVKEAV QNAVETLKSL GAVVEEVSLP
310 320 330 340 350
NTKFGIPSYY VIASSEASSN LSRFDGIRYG YHSKEAHSLE ELYKMSRSEG
360 370 380 390 400
FGKEVKRRIF LGTFALSSGY YDAYYKKSQK VRTLIKNDFD KVFENYDVVV
410 420 430 440 450
GPTAPTTAFN LGEEIDDPLT MYANDLLTTP VNLAGLPGIS VPCGQSNGRP
460 470 480
IGLQFIGKPF DEKTLYRVAY QYETQYNLHD VYEKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000017 Genomic DNA Translation: BAB58062.1 |
RefSeqi | WP_000027919.1, NC_002758.2 |
Genome annotation databases
EnsemblBacteriai | BAB58062; BAB58062; SAV1900 |
KEGGi | sav:SAV1900 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000017 Genomic DNA Translation: BAB58062.1 |
RefSeqi | WP_000027919.1, NC_002758.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DF4 | X-ray | 3.20 | A | 1-485 | [»] | |
2DQN | X-ray | 2.55 | A | 1-485 | [»] | |
2F2A | X-ray | 2.30 | A | 1-485 | [»] | |
2G5H | X-ray | 2.50 | A | 1-485 | [»] | |
2G5I | X-ray | 3.35 | A | 1-485 | [»] | |
3IP4 | X-ray | 1.90 | A | 1-485 | [»] | |
SMRi | P63488 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
2D gel databases
World-2DPAGEi | 0002:P63488 |
Proteomic databases
PaxDbi | P63488 |
Genome annotation databases
EnsemblBacteriai | BAB58062; BAB58062; SAV1900 |
KEGGi | sav:SAV1900 |
Phylogenomic databases
HOGENOMi | CLU_009600_0_3_9 |
OMAi | MYLSDIF |
PhylomeDBi | P63488 |
Enzyme and pathway databases
BioCyci | SAUR158878:SAV_RS10410-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P63488 |
Family and domain databases
Gene3Di | 3.90.1300.10, 1 hit |
HAMAPi | MF_00120, GatA, 1 hit |
InterProi | View protein in InterPro IPR000120, Amidase IPR020556, Amidase_CS IPR023631, Amidase_dom IPR036928, AS_sf IPR004412, GatA |
PANTHERi | PTHR11895, PTHR11895, 1 hit |
Pfami | View protein in Pfam PF01425, Amidase, 1 hit |
SUPFAMi | SSF75304, SSF75304, 1 hit |
TIGRFAMsi | TIGR00132, gatA, 1 hit |
PROSITEi | View protein in PROSITE PS00571, AMIDASES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GATA_STAAM | |
Accessioni | P63488Primary (citable) accession number: P63488 Secondary accession number(s): Q99SY6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | December 2, 2020 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structureDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families