UniProtKB - P63386 (MLAF_ECOLI)
Protein
Intermembrane phospholipid transport system ATP-binding protein MlaF
Gene
mlaF
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex MlaFEDB, which is involved in a phospholipid transport pathway that maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane. Responsible for energy coupling to the transport system.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 41 – 48 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: EcoCyc
- ATP binding Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:YRBF-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.27.3, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Intermembrane phospholipid transport system ATP-binding protein MlaFCurated (EC:7.6.2.-1 Publication) |
Gene namesi | Name:mlaF1 Publication Synonyms:yrbF Ordered Locus Names:b3195, JW3162 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- protein-containing complex Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutation leads to accumulation of phospholipid in the outer leaflet of the outer membrane and increased outer membrane permeability. It confers sensitivity to SDS-EDTA.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093171 | 1 – 269 | Intermembrane phospholipid transport system ATP-binding protein MlaFAdd BLAST | 269 |
Proteomic databases
jPOSTi | P63386 |
PaxDbi | P63386 |
PRIDEi | P63386 |
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (MlaF), two transmembrane proteins (MlaE), two cytoplasmic solute-binding proteins (MlaB) and six periplasmic solute-binding proteins (MlaD).
1 Publication2 PublicationsBinary interactionsi
P63386
With | #Exp. | IntAct |
---|---|---|
groL [P0A6F5] | 4 | EBI-561408,EBI-543750 |
Protein-protein interaction databases
BioGRIDi | 4263401, 121 interactors 852042, 1 interactor |
ComplexPortali | CPX-3464, MlaFEDB lipid transport complex |
DIPi | DIP-35865N |
IntActi | P63386, 9 interactors |
STRINGi | 511145.b3195 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 245 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 237 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1127, Bacteria |
HOGENOMi | CLU_000604_1_22_6 |
InParanoidi | P63386 |
PhylomeDBi | P63386 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR030296, MlaF/Mkl IPR027417, P-loop_NTPase |
PANTHERi | PTHR43023:SF6, PTHR43023:SF6, 1 hit |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P63386-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEQSVANLVD MRDVSFTRGN RCIFDNISLT VPRGKITAIM GPSGIGKTTL
60 70 80 90 100
LRLIGGQIAP DHGEILFDGE NIPAMSRSRL YTVRKRMSML FQSGALFTDM
110 120 130 140 150
NVFDNVAYPL REHTQLPAPL LHSTVMMKLE AVGLRGAAKL MPSELSGGMA
160 170 180 190 200
RRAALARAIA LEPDLIMFDE PFVGQDPITM GVLVKLISEL NSALGVTCVV
210 220 230 240 250
VSHDVPEVLS IADHAWILAD KKIVAHGSAQ ALQANPDPRV RQFLDGIADG
260
PVPFRYPAGD YHADLLPGS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57996.1 U00096 Genomic DNA Translation: AAC76227.1 AP009048 Genomic DNA Translation: BAE77239.1 |
PIRi | E65110 |
RefSeqi | NP_417662.1, NC_000913.3 WP_000438245.1, NZ_STEB01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC76227; AAC76227; b3195 BAE77239; BAE77239; BAE77239 |
GeneIDi | 58460030 947729 |
KEGGi | ecj:JW3162 eco:b3195 |
PATRICi | fig|1411691.4.peg.3536 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18997 Genomic DNA Translation: AAA57996.1 U00096 Genomic DNA Translation: AAC76227.1 AP009048 Genomic DNA Translation: BAE77239.1 |
PIRi | E65110 |
RefSeqi | NP_417662.1, NC_000913.3 WP_000438245.1, NZ_STEB01000012.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7CGE | electron microscopy | 2.90 | B/E | 1-269 | [»] | |
7CGN | electron microscopy | 4.30 | B/E | 1-269 | [»] | |
7CH0 | electron microscopy | 3.70 | B/E | 1-269 | [»] | |
SMRi | P63386 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263401, 121 interactors 852042, 1 interactor |
ComplexPortali | CPX-3464, MlaFEDB lipid transport complex |
DIPi | DIP-35865N |
IntActi | P63386, 9 interactors |
STRINGi | 511145.b3195 |
Protein family/group databases
TCDBi | 3.A.1.27.3, the atp-binding cassette (abc) superfamily |
Proteomic databases
jPOSTi | P63386 |
PaxDbi | P63386 |
PRIDEi | P63386 |
Genome annotation databases
EnsemblBacteriai | AAC76227; AAC76227; b3195 BAE77239; BAE77239; BAE77239 |
GeneIDi | 58460030 947729 |
KEGGi | ecj:JW3162 eco:b3195 |
PATRICi | fig|1411691.4.peg.3536 |
Organism-specific databases
EchoBASEi | EB2653 |
Phylogenomic databases
eggNOGi | COG1127, Bacteria |
HOGENOMi | CLU_000604_1_22_6 |
InParanoidi | P63386 |
PhylomeDBi | P63386 |
Enzyme and pathway databases
BioCyci | EcoCyc:YRBF-MONOMER |
Miscellaneous databases
PROi | PR:P63386 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR030296, MlaF/Mkl IPR027417, P-loop_NTPase |
PANTHERi | PTHR43023:SF6, PTHR43023:SF6, 1 hit |
Pfami | View protein in Pfam PF00005, ABC_tran, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MLAF_ECOLI | |
Accessioni | P63386Primary (citable) accession number: P63386 Secondary accession number(s): P45393, Q2M917 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families