UniProtKB - P63330 (PP2AA_MOUSE)
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Ppp2ca
Functioni
Miscellaneous
Catalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 57 | Manganese 1By similarity | 1 | |
Metal bindingi | 59 | Manganese 1By similarity | 1 | |
Metal bindingi | 85 | Manganese 1By similarity | 1 | |
Metal bindingi | 85 | Manganese 2By similarity | 1 | |
Metal bindingi | 117 | Manganese 2By similarity | 1 | |
Active sitei | 118 | Proton donorBy similarity | 1 | |
Metal bindingi | 167 | Manganese 2By similarity | 1 | |
Metal bindingi | 241 | Manganese 2By similarity | 1 |
GO - Molecular functioni
- beta-2 adrenergic receptor binding Source: MGI
- enzyme binding Source: MGI
- GABA receptor binding Source: MGI
- identical protein binding Source: MGI
- ion channel binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- protein antigen binding Source: MGI
- protein-containing complex binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein kinase B binding Source: MGI
- protein kinase binding Source: MGI
- protein phosphatase 2A binding Source: MGI
- protein phosphatase binding Source: MGI
- protein serine/threonine phosphatase activity Source: UniProtKB
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
- tau protein binding Source: MGI
GO - Biological processi
- cellular response to glucose stimulus Source: MGI
- meiotic cell cycle Source: UniProtKB-KW
- mesoderm development Source: MGI
- negative regulation of calcium ion transmembrane transporter activity Source: MGI
- negative regulation of epithelial to mesenchymal transition Source: MGI
- negative regulation of protein import into nucleus Source: MGI
- negative regulation of protein phosphorylation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- peptidyl-threonine dephosphorylation Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- positive regulation of microtubule binding Source: MGI
- positive regulation of phosphoprotein phosphatase activity Source: MGI
- positive regulation of protein dephosphorylation Source: MGI
- positive regulation of protein serine/threonine kinase activity Source: MGI
- protein dephosphorylation Source: MGI
- regulation of cell cycle Source: MGI
- regulation of protein autophosphorylation Source: MGI
- regulation of protein catabolic process Source: MGI
- regulation of protein phosphorylation Source: MGI
- regulation of signaling receptor activity Source: MGI
- response to lead ion Source: MGI
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Meiosis |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-113501, Inhibition of replication initiation of damaged DNA by RB1/E2F1 R-MMU-1295596, Spry regulation of FGF signaling R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-180024, DARPP-32 events R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-196299, Beta-catenin phosphorylation cascade R-MMU-198753, ERK/MAPK targets R-MMU-202670, ERKs are inactivated R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2995383, Initiation of Nuclear Envelope (NE) Reformation R-MMU-389513, CTLA4 inhibitory signaling R-MMU-432142, Platelet sensitization by LDL R-MMU-4641262, Disassembly of the destruction complex and recruitment of AXIN to the membrane R-MMU-5663220, RHO GTPases Activate Formins R-MMU-5673000, RAF activation R-MMU-5675221, Negative regulation of MAPK pathway R-MMU-6804757, Regulation of TP53 Degradation R-MMU-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-MMU-68877, Mitotic Prometaphase R-MMU-69231, Cyclin D associated events in G1 R-MMU-69273, Cyclin A/B1/B2 associated events during G2/M transition R-MMU-9648025, EML4 and NUDC in mitotic spindle formation R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (EC:3.1.3.16By similarity)Short name: PP2A-alpha |
Gene namesi | Name:Ppp2ca |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1321159, Ppp2ca |
Subcellular locationi
Cytoskeleton
- spindle pole By similarity
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm 1 Publication
- centromere 1 Publication
Note: In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles (By similarity). Centromeric localization requires the presence of SGO2.By similarity
Cytoskeleton
- spindle pole Source: UniProtKB-SubCell
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- chromosome, centromeric region Source: UniProtKB
- cytoplasm Source: MGI
- membrane raft Source: UniProtKB
- neuron projection Source: MGI
- postsynaptic density Source: MGI
- protein phosphatase type 2A complex Source: MGI
- synapse Source: SynGO
- terminal bouton Source: MGI
Keywords - Cellular componenti
Centromere, Chromosome, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 307 | Y → Q: Loss of trimeric subunit ABC assembly. 1 Publication | 1 | |
Mutagenesisi | 309 | L → A: Loss of binding to PP2A B-alpha regulatory subunit. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058840 | 1 – 309 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoformAdd BLAST | 309 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 307 | Phosphotyrosine1 Publication | 1 | |
Modified residuei | 309 | Leucine methyl ester1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P63330 |
jPOSTi | P63330 |
MaxQBi | P63330 |
PaxDbi | P63330 |
PeptideAtlasi | P63330 |
PRIDEi | P63330 |
PTM databases
iPTMneti | P63330 |
PhosphoSitePlusi | P63330 |
SwissPalmi | P63330 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020349, Expressed in cerebellum and 320 other tissues |
Genevisiblei | P63330, MM |
Interactioni
Subunit structurei
PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
Interacts with NXN; the interaction is direct.
Interacts with TP53, SGO1 and SGO2.
Interacts with AXIN1; the interaction dephosphorylates AXIN1 (By similarity).
Interacts with PIM3; this interaction promotes dephosphorylation, ubiquitination and proteasomal degradation of PIM3 (By similarity).
Interacts with RAF1.
Interacts with GSK3B (via C2 domain) (By similarity). Interaction with IGBP1 protects unassembled PPP2CA from degradative ubiquitination (By similarity).
Interacts with KCTD20 (PubMed:24156551).
Interacts with BTBD10 (PubMed:18160256).
Interacts with MFHAS1; retains PPP2CA into the cytoplasm and excludes it from the nucleus (By similarity).
Interacts with PABIR1/FAM122A (By similarity).
Interacts with ADCY8; interaction is phosphatase activity-dependent; antagonizes interaction between ADCY8 and calmodulin (PubMed:16258073).
Interacts with CRTC3 (when phosphorylated at 'Ser-391') (PubMed:30611118).
Interacts with SPRY2; the interaction is inhibited by TESK1 interaction with SPRY2, possibly by vesicular sequestration of SPRY2 (By similarity).
Interacts with TRAF3IP3 (By similarity).
By similarity4 PublicationsBinary interactionsi
Hide detailsGO - Molecular functioni
- beta-2 adrenergic receptor binding Source: MGI
- enzyme binding Source: MGI
- GABA receptor binding Source: MGI
- identical protein binding Source: MGI
- ion channel binding Source: MGI
- protein antigen binding Source: MGI
- protein C-terminus binding Source: MGI
- protein domain specific binding Source: MGI
- protein heterodimerization activity Source: UniProtKB
- protein kinase B binding Source: MGI
- protein kinase binding Source: MGI
- protein phosphatase 2A binding Source: MGI
- protein phosphatase binding Source: MGI
- tau protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 202341, 104 interactors |
CORUMi | P63330 |
IntActi | P63330, 67 interactors |
MINTi | P63330 |
STRINGi | 10090.ENSMUSP00000020608 |
Miscellaneous databases
RNActi | P63330, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0371, Eukaryota |
GeneTreei | ENSGT00550000074618 |
HOGENOMi | CLU_004962_0_5_1 |
InParanoidi | P63330 |
OMAi | MKVRYPN |
OrthoDBi | 808922at2759 |
PhylomeDBi | P63330 |
TreeFami | TF105559 |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MDEKLFTKEL DQWIEQLNEC KQLSESQVKS LCEKAKEILT KESNVQEVRC
60 70 80 90 100
PVTVCGDVHG QFHDLMELFR IGGKSPDTNY LFMGDYVDRG YYSVETVTLL
110 120 130 140 150
VALKVRYRER ITILRGNHES RQITQVYGFY DECLRKYGNA NVWKYFTDLF
160 170 180 190 200
DYLPLTALVD GQIFCLHGGL SPSIDTLDHI RALDRLQEVP HEGPMCDLLW
210 220 230 240 250
SDPDDRGGWG ISPRGAGYTF GQDISETFNH ANGLTLVSRA HQLVMEGYNW
260 270 280 290 300
CHDRNVVTIF SAPNYCYRCG NQAAIMELDD TLKYSFLQFD PAPRRGEPHV
TRRTPDYFL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | L → P in AAD12587 (PubMed:9920888).Curated | 1 | |
Sequence conflicti | 73 | G → V in AAD12587 (PubMed:9920888).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF076192 mRNA Translation: AAD12587.1 Z67745 mRNA Translation: CAA91558.1 AK076110 mRNA Translation: BAC36190.1 AK172644 mRNA Translation: BAE43111.1 AL935177 Genomic DNA No translation available. BC003856 mRNA Translation: AAH03856.1 BC054458 mRNA Translation: AAH54458.1 |
CCDSi | CCDS24666.1 |
RefSeqi | NP_062284.1, NM_019411.4 |
Genome annotation databases
Ensembli | ENSMUST00000020608; ENSMUSP00000020608; ENSMUSG00000020349 |
GeneIDi | 19052 |
KEGGi | mmu:19052 |
UCSCi | uc007ivb.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF076192 mRNA Translation: AAD12587.1 Z67745 mRNA Translation: CAA91558.1 AK076110 mRNA Translation: BAC36190.1 AK172644 mRNA Translation: BAE43111.1 AL935177 Genomic DNA No translation available. BC003856 mRNA Translation: AAH03856.1 BC054458 mRNA Translation: AAH54458.1 |
CCDSi | CCDS24666.1 |
RefSeqi | NP_062284.1, NM_019411.4 |
3D structure databases
SMRi | P63330 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202341, 104 interactors |
CORUMi | P63330 |
IntActi | P63330, 67 interactors |
MINTi | P63330 |
STRINGi | 10090.ENSMUSP00000020608 |
PTM databases
iPTMneti | P63330 |
PhosphoSitePlusi | P63330 |
SwissPalmi | P63330 |
Proteomic databases
EPDi | P63330 |
jPOSTi | P63330 |
MaxQBi | P63330 |
PaxDbi | P63330 |
PeptideAtlasi | P63330 |
PRIDEi | P63330 |
Protocols and materials databases
Antibodypediai | 3550, 634 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000020608; ENSMUSP00000020608; ENSMUSG00000020349 |
GeneIDi | 19052 |
KEGGi | mmu:19052 |
UCSCi | uc007ivb.1, mouse |
Organism-specific databases
CTDi | 5515 |
MGIi | MGI:1321159, Ppp2ca |
Phylogenomic databases
eggNOGi | KOG0371, Eukaryota |
GeneTreei | ENSGT00550000074618 |
HOGENOMi | CLU_004962_0_5_1 |
InParanoidi | P63330 |
OMAi | MKVRYPN |
OrthoDBi | 808922at2759 |
PhylomeDBi | P63330 |
TreeFami | TF105559 |
Enzyme and pathway databases
Reactomei | R-MMU-113501, Inhibition of replication initiation of damaged DNA by RB1/E2F1 R-MMU-1295596, Spry regulation of FGF signaling R-MMU-141444, Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-MMU-180024, DARPP-32 events R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-196299, Beta-catenin phosphorylation cascade R-MMU-198753, ERK/MAPK targets R-MMU-202670, ERKs are inactivated R-MMU-2467813, Separation of Sister Chromatids R-MMU-2500257, Resolution of Sister Chromatid Cohesion R-MMU-2995383, Initiation of Nuclear Envelope (NE) Reformation R-MMU-389513, CTLA4 inhibitory signaling R-MMU-432142, Platelet sensitization by LDL R-MMU-4641262, Disassembly of the destruction complex and recruitment of AXIN to the membrane R-MMU-5663220, RHO GTPases Activate Formins R-MMU-5673000, RAF activation R-MMU-5675221, Negative regulation of MAPK pathway R-MMU-6804757, Regulation of TP53 Degradation R-MMU-6811558, PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling R-MMU-68877, Mitotic Prometaphase R-MMU-69231, Cyclin D associated events in G1 R-MMU-69273, Cyclin A/B1/B2 associated events during G2/M transition R-MMU-9648025, EML4 and NUDC in mitotic spindle formation R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 19052, 7 hits in 16 CRISPR screens |
ChiTaRSi | Ppp2ca, mouse |
PROi | PR:P63330 |
RNActi | P63330, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020349, Expressed in cerebellum and 320 other tissues |
Genevisiblei | P63330, MM |
Family and domain databases
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR006186, Ser/Thr-sp_prot-phosphatase |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP2AA_MOUSE | |
Accessioni | P63330Primary (citable) accession number: P63330 Secondary accession number(s): O88591, P13353, Q5SNY5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families