UniProtKB - P63329 (PP2BA_RAT)
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Ppp3ca
Functioni
Catalytic activityi
Cofactori
Protein has several cofactor binding sites:- Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
- Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 90 | IronBy similarity | 1 | |
Metal bindingi | 92 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 118 | IronBy similarity | 1 | |
Metal bindingi | 118 | ZincBy similarity | 1 | |
Metal bindingi | 150 | ZincBy similarity | 1 | |
Active sitei | 151 | Proton donorBy similarity | 1 | |
Metal bindingi | 199 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 281 | Zinc; via pros nitrogenBy similarity | 1 |
GO - Molecular functioni
- ATPase binding Source: RGD
- calcium-dependent protein serine/threonine phosphatase activity Source: RGD
- calmodulin binding Source: RGD
- calmodulin-dependent protein phosphatase activity Source: UniProtKB
- cyclosporin A binding Source: RGD
- enzyme binding Source: RGD
- metal ion binding Source: UniProtKB-KW
- phosphoprotein phosphatase activity Source: RGD
- protein-containing complex binding Source: RGD
- protein dimerization activity Source: RGD
- protein serine/threonine phosphatase activity Source: RGD
- protein serine phosphatase activity Source: UniProtKB-EC
- protein threonine phosphatase activity Source: UniProtKB-EC
GO - Biological processi
- aging Source: RGD
- brain development Source: RGD
- calcineurin-mediated signaling Source: RGD
- calcineurin-NFAT signaling cascade Source: RGD
- calcium ion transport Source: RGD
- calcium-mediated signaling Source: RGD
- cardiac muscle hypertrophy in response to stress Source: RGD
- cellular response to glucose stimulus Source: BHF-UCL
- dephosphorylation Source: RGD
- excitatory postsynaptic potential Source: RGD
- G1/S transition of mitotic cell cycle Source: RGD
- modulation of chemical synaptic transmission Source: RGD
- multicellular organismal response to stress Source: RGD
- negative regulation of calcium ion-dependent exocytosis Source: BHF-UCL
- negative regulation of chromatin binding Source: RGD
- negative regulation of dendrite morphogenesis Source: RGD
- negative regulation of gene expression Source: RGD
- negative regulation of insulin secretion Source: BHF-UCL
- negative regulation of production of miRNAs involved in gene silencing by miRNA Source: RGD
- peptidyl-serine dephosphorylation Source: RGD
- positive regulation of cardiac muscle hypertrophy Source: RGD
- positive regulation of cardiac muscle hypertrophy in response to stress Source: RGD
- positive regulation of cell adhesion Source: RGD
- positive regulation of cell migration Source: RGD
- positive regulation of connective tissue replacement Source: RGD
- positive regulation of DNA-binding transcription factor activity Source: RGD
- positive regulation of endocytosis Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- postsynaptic modulation of chemical synaptic transmission Source: RGD
- protein dephosphorylation Source: UniProtKB
- protein import into nucleus Source: RGD
- response to amphetamine Source: RGD
- response to calcium ion Source: UniProtKB
- skeletal muscle fiber development Source: RGD
- transition between fast and slow fiber Source: RGD
Keywordsi
Molecular function | Calmodulin-binding, Hydrolase, Protein phosphatase |
Ligand | Iron, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.1.3.16, 5301 |
Reactomei | R-RNO-2025928, Calcineurin activates NFAT R-RNO-2871809, FCERI mediated Ca+2 mobilization R-RNO-4086398, Ca2+ pathway R-RNO-5607763, CLEC7A (Dectin-1) induces NFAT activation |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform (EC:3.1.3.162 Publications)Alternative name(s): CAM-PRP catalytic subunit Calmodulin-dependent calcineurin A subunit alpha isoform |
Gene namesi | Name:Ppp3ca Synonyms:Calna |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3382, Ppp3ca |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
- sarcolemma 1 Publication
Other locations
- Z line 1 Publication
- dendritic spine By similarity
Note: Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle (PubMed:11114196). Recruited to the cell membrane by scaffold protein AKAP5 following L-type Ca2+-channel activation (By similarity).By similarity1 Publication
Cytosol
- cytosol Source: RGD
Mitochondrion
- mitochondrion Source: RGD
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: RGD
Plasma Membrane
- cytoplasmic side of plasma membrane Source: Ensembl
- plasma membrane Source: RGD
- sarcolemma Source: UniProtKB-SubCell
Other locations
- calcineurin complex Source: RGD
- cytoplasm Source: RGD
- dendritic spine Source: RGD
- glutamatergic synapse Source: RGD
- Schaffer collateral - CA1 synapse Source: RGD
- synapse Source: RGD
- Z disc Source: RGD
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000058824 | 2 – 521 | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoformAdd BLAST | 520 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 224 | 3'-nitrotyrosineBy similarity | 1 | |
Modified residuei | 469 | PhosphoserineCombined sources | 1 | |
Modified residuei | 492 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Nitration, PhosphoproteinProteomic databases
jPOSTi | P63329 |
PaxDbi | P63329 |
PRIDEi | P63329 |
PTM databases
iPTMneti | P63329 |
PhosphoSitePlusi | P63329 |
SwissPalmi | P63329 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000009882, Expressed in Ammon's horn and 20 other tissues |
Genevisiblei | P63329, RN |
Interactioni
Subunit structurei
Forms a complex composed of a calmodulin-dependent catalytic subunit (also known as calcineurin A) and a regulatory Ca2+-binding subunit (also known as calcineurin B) (By similarity). There are three catalytic subunits, each encoded by a separate gene (PPP3CA, PPP3CB, and PPP3CC) and two regulatory subunits which are also encoded by separate genes (PPP3R1 and PPP3R2). In response to an increase in Ca2+ intracellular levels, forms a complex composed of PPP3CA/calcineurin A, calcineurin B and calmodulin (By similarity).
Interacts (via calcineurin B binding domain) with regulatory subunit PPP3R1/calcineurin B (By similarity).
Interacts (via calmodulin-binding domain) with calmodulin; the interaction depends on calmodulin binding to Ca2+ (By similarity).
Forms a complex composed of MYOZ2 and ACTN1 (By similarity). Within the complex interacts with MYOZ2 (By similarity).
Interacts with MYOZ1 (By similarity).
Interacts with MYOZ3 (By similarity).
Interacts with CIB1; the interaction increases upon cardiomyocyte hypertrophy (By similarity).
Interacts with CHP1 and CHP2 (PubMed:10593895, PubMed:18815128).
Interacts with CRTC1.
Interacts with CRTC2 (By similarity).
Interacts with DNM1L; the interaction dephosphorylates DNM1L and promotes its translocation to mitochondria (By similarity).
Interacts with CMYA5; this interaction represses calcineurin activity in muscle (By similarity).
Interacts (constitutively active form) with SYNPO2 (By similarity).
Interacts with scaffold protein AKAP5 (via IAIIIT motif); the interaction recruits PPP3CA to the plasma membrane following L-type Ca2+-channel activation (By similarity).
Interacts with NFATC2 (By similarity).
Interacts with RCAN3 (By similarity).
Interacts with PPIA.
Interacts with RCAN1 (By similarity).
Interacts with UNC119 (By similarity).
By similarity2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 352 | Interaction with PxVP motif in substrateBy similarity | 1 |
Binary interactionsi
Hide detailsP63329
With | #Exp. | IntAct |
---|---|---|
Cacna1c [P22002] | 5 | EBI-7022944,EBI-1185084 |
Cacng8 [Q8VHW5] | 6 | EBI-7022944,EBI-9086576 |
GO - Molecular functioni
- ATPase binding Source: RGD
- calmodulin binding Source: RGD
- enzyme binding Source: RGD
- protein dimerization activity Source: RGD
Protein-protein interaction databases
BioGRIDi | 246806, 5 interactors |
CORUMi | P63329 |
IntActi | P63329, 6 interactors |
MINTi | P63329 |
STRINGi | 10116.ENSRNOP00000049674 |
Structurei
Secondary structure
3D structure databases
BMRBi | P63329 |
SMRi | P63329 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 56 – 340 | CatalyticCuratedAdd BLAST | 285 | |
Regioni | 327 – 336 | Interaction with PxIxIF motif in substrateBy similarity | 10 | |
Regioni | 341 – 369 | Calcineurin B bindingBy similarityAdd BLAST | 29 | |
Regioni | 392 – 406 | Calmodulin-bindingBy similarityAdd BLAST | 15 | |
Regioni | 407 – 414 | Autoinhibitory segmentBy similarity | 8 | |
Regioni | 465 – 487 | Autoinhibitory domain1 PublicationAdd BLAST | 23 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 307 – 311 | SAPNY motifBy similarity | 5 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0375, Eukaryota |
GeneTreei | ENSGT00940000156306 |
HOGENOMi | CLU_004962_6_0_1 |
InParanoidi | P63329 |
OMAi | WSLKIWY |
OrthoDBi | 463522at2759 |
PhylomeDBi | P63329 |
Family and domain databases
CDDi | cd07416, MPP_PP2B, 1 hit |
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR041751, MPP_PP2B IPR043360, PP2B IPR006186, Ser/Thr-sp_prot-phosphatase |
PANTHERi | PTHR45673, PTHR45673, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSEPKAIDPK LSTTDRVVKA VPFPPSHRLT AKEVFDNDGK PRVDILKAHL
60 70 80 90 100
MKEGRLEESV ALRIITEGAS ILRQEKNLLD IDAPVTVCGD IHGQFFDLMK
110 120 130 140 150
LFEVGGSPAN TRYLFLGDYV DRGYFSIECV LYLWALKILY PKTLFLLRGN
160 170 180 190 200
HECRHLTEYF TFKQECKIKY SERVYDACMD AFDCLPLAAL MNQQFLCVHG
210 220 230 240 250
GLSPEINTLD DIRKLDRFKE PPAYGPMCDI LWSDPLEDFG NEKTQEHFTH
260 270 280 290 300
NTVRGCSYFY SYPAVCDFLQ HNNLLSILRA HEAQDAGYRM YRKSQTTGFP
310 320 330 340 350
SLITIFSAPN YLDVYNNKAA VLKYENNVMN IRQFNCSPHP YWLPNFMDVF
360 370 380 390 400
TWSLPFVGEK VTEMLVNVLN ICSDDELGSE EDGFDGATAA ARKEVIRNKI
410 420 430 440 450
RAIGKMARVF SVLREESESV LTLKGLTPTG MLPSGVLSGG KQTLQSATVE
460 470 480 490 500
AIEADEAIKG FSPQHKITSF EEAKGLDRIN ERMPPRRDAM PSDANLNSIN
510 520
KALASETNGT DSNGSNSSNI Q
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018564 | 448 – 457 | Missing in isoform 2. 1 Publication | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90035 mRNA Translation: BAA14083.1 M29275 mRNA Translation: AAA40940.1 X57115 mRNA Translation: CAA40398.2 D10480 Genomic DNA Translation: BAA01283.1 M58440 mRNA Translation: AAA41914.1 |
PIRi | A33264 |
RefSeqi | NP_058737.1, NM_017041.1 [P63329-1] XP_006233425.1, XM_006233363.3 [P63329-2] |
Genome annotation databases
Ensembli | ENSRNOT00000047975; ENSRNOP00000049674; ENSRNOG00000009882 [P63329-1] |
GeneIDi | 24674 |
KEGGi | rno:24674 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90035 mRNA Translation: BAA14083.1 M29275 mRNA Translation: AAA40940.1 X57115 mRNA Translation: CAA40398.2 D10480 Genomic DNA Translation: BAA01283.1 M58440 mRNA Translation: AAA41914.1 |
PIRi | A33264 |
RefSeqi | NP_058737.1, NM_017041.1 [P63329-1] XP_006233425.1, XM_006233363.3 [P63329-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4IL1 | X-ray | 3.00 | A/B/C/D | 1-492 | [»] | |
BMRBi | P63329 | |||||
SMRi | P63329 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 246806, 5 interactors |
CORUMi | P63329 |
IntActi | P63329, 6 interactors |
MINTi | P63329 |
STRINGi | 10116.ENSRNOP00000049674 |
PTM databases
iPTMneti | P63329 |
PhosphoSitePlusi | P63329 |
SwissPalmi | P63329 |
Proteomic databases
jPOSTi | P63329 |
PaxDbi | P63329 |
PRIDEi | P63329 |
Genome annotation databases
Ensembli | ENSRNOT00000047975; ENSRNOP00000049674; ENSRNOG00000009882 [P63329-1] |
GeneIDi | 24674 |
KEGGi | rno:24674 |
Organism-specific databases
CTDi | 5530 |
RGDi | 3382, Ppp3ca |
Phylogenomic databases
eggNOGi | KOG0375, Eukaryota |
GeneTreei | ENSGT00940000156306 |
HOGENOMi | CLU_004962_6_0_1 |
InParanoidi | P63329 |
OMAi | WSLKIWY |
OrthoDBi | 463522at2759 |
PhylomeDBi | P63329 |
Enzyme and pathway databases
BRENDAi | 3.1.3.16, 5301 |
Reactomei | R-RNO-2025928, Calcineurin activates NFAT R-RNO-2871809, FCERI mediated Ca+2 mobilization R-RNO-4086398, Ca2+ pathway R-RNO-5607763, CLEC7A (Dectin-1) induces NFAT activation |
Miscellaneous databases
PROi | PR:P63329 |
Gene expression databases
Bgeei | ENSRNOG00000009882, Expressed in Ammon's horn and 20 other tissues |
Genevisiblei | P63329, RN |
Family and domain databases
CDDi | cd07416, MPP_PP2B, 1 hit |
Gene3Di | 3.60.21.10, 1 hit |
InterProi | View protein in InterPro IPR004843, Calcineurin-like_PHP_ApaH IPR029052, Metallo-depent_PP-like IPR041751, MPP_PP2B IPR043360, PP2B IPR006186, Ser/Thr-sp_prot-phosphatase |
PANTHERi | PTHR45673, PTHR45673, 1 hit |
Pfami | View protein in Pfam PF00149, Metallophos, 1 hit |
PRINTSi | PR00114, STPHPHTASE |
SMARTi | View protein in SMART SM00156, PP2Ac, 1 hit |
PROSITEi | View protein in PROSITE PS00125, SER_THR_PHOSPHATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PP2BA_RAT | |
Accessioni | P63329Primary (citable) accession number: P63329 Secondary accession number(s): P12816 Q9Z1I5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | April 7, 2021 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families