UniProtKB - P63321 (RALA_MOUSE)
Ras-related protein Ral-A
Rala
Functioni
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity).
The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108).
During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (By similarity).
During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B-CDK1 (By similarity).
By similarity1 PublicationCatalytic activityi
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 21 – 28 | GTPBy similarity | 8 | |
Nucleotide bindingi | 68 – 72 | GTPBy similarity | 5 | |
Nucleotide bindingi | 127 – 130 | GTPBy similarity | 4 |
GO - Molecular functioni
- ATPase binding Source: MGI
- Edg-2 lysophosphatidic acid receptor binding Source: UniProtKB
- GDP binding Source: MGI
- G protein activity Source: UniProtKB-EC
- GTPase activity Source: Reactome
- GTP binding Source: MGI
- myosin binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
GO - Biological processi
- actin cytoskeleton reorganization Source: MGI
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- establishment of protein localization to mitochondrion Source: UniProtKB
- exocytosis Source: UniProtKB-KW
- membrane raft localization Source: UniProtKB
- neural tube closure Source: CACAO
- positive regulation of filopodium assembly Source: MGI
- positive regulation of mitochondrial fission Source: UniProtKB
- Ras protein signal transduction Source: GO_Central
- regulation of exocytosis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase |
Biological process | Cell cycle, Cell division, Exocytosis |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Rala Synonyms:Ral, Ral-a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1927243, Rala |
VEuPathDBi | HostDB:ENSMUSG00000008859 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Other locations
- Cleavage furrow By similarity
- Midbody ring By similarity
Note: Predominantly at the cell surface in the absence of LPA. In the presence of LPA, colocalizes with LPAR1 and LPAR2 in endocytic vesicles. May colocalize with CNTRL/centriolin at the midbody ring. However, localization at the midbody at late cytokinesis was not confirmed. Relocalizes to the mitochondrion during mitosis where it regulates mitochondrial fission.By similarity
Mitochondrion
- mitochondrion Source: UniProtKB
Plasma Membrane
- cleavage furrow Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- cell surface Source: MGI
- cytoplasmic vesicle membrane Source: Reactome
- endocytic vesicle Source: Ensembl
- Flemming body Source: UniProtKB-SubCell
- focal adhesion Source: MGI
- myelin sheath Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082694 | 1 – 203 | Ras-related protein Ral-AAdd BLAST | 203 | |
PropeptideiPRO_0000281345 | 204 – 206 | Removed in mature formBy similarity | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 194 | PhosphoserineBy similarity | 1 | |
Modified residuei | 203 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 203 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | P63321 |
jPOSTi | P63321 |
MaxQBi | P63321 |
PaxDbi | P63321 |
PeptideAtlasi | P63321 |
PRIDEi | P63321 |
ProteomicsDBi | 253169 |
PTM databases
iPTMneti | P63321 |
PhosphoSitePlusi | P63321 |
SwissPalmi | P63321 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000008859, Expressed in vestibular organ and 330 other tissues |
Genevisiblei | P63321, MM |
Interactioni
Subunit structurei
Interacts (via effector domain) with RALBP1; during mitosis, recruits RALBP1 to the mitochondrion where it promotes DNM1L phosphorylation and mitochondrial fission (By similarity).
Interacts with EXOC2/Sec5 and EXOC8/Exo84; binding to EXOC2 and EXOC8 is mutually exclusive.
Interacts with Clostridium exoenzyme C3.
Interacts with RALGPS1.
Interacts with LPAR1 and LPAR2.
Interacts with GRK2 in response to LPAR1 activation. RALA and GRK2 binding to LPAR1 is mutually exclusive (By similarity).
Interacts with CDC42 (By similarity).
By similarityGO - Molecular functioni
- ATPase binding Source: MGI
- Edg-2 lysophosphatidic acid receptor binding Source: UniProtKB
- myosin binding Source: MGI
- ubiquitin protein ligase binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 207790, 7 interactors |
IntActi | P63321, 1 interactor |
STRINGi | 10090.ENSMUSP00000009003 |
Miscellaneous databases
RNActi | P63321, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 43 – 51 | Effector region | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000155142 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P63321 |
OMAi | QSRAQQW |
OrthoDBi | 1259506at2759 |
PhylomeDBi | P63321 |
TreeFami | TF312796 |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR028412, Ral IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit PTHR24070:SF174, PTHR24070:SF174, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS
60 70 80 90 100
YRKKVVLDGE EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES
110 120 130 140 150
FAATADFREQ ILRVKEDENV PFLLVGNKSD LEDKRQVSVE EAKNRADQWN
160 170 180 190 200
VNYVETSAKT RANVDKVFFD LMREIRARKM EDSKEKNGKK KRKSLAKRIR
ERCCIL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1Y7VL93 | A0A1Y7VL93_MOUSE | Small monomeric GTPase | Rala | 137 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z48587 mRNA Translation: CAA88488.1 AF244951 mRNA Translation: AAG23136.1 BC031741 mRNA Translation: AAH31741.1 |
CCDSi | CCDS26255.1 |
RefSeqi | NP_062364.3, NM_019491.5 |
Genome annotation databases
Ensembli | ENSMUST00000009003; ENSMUSP00000009003; ENSMUSG00000008859 |
GeneIDi | 56044 |
KEGGi | mmu:56044 |
UCSCi | uc007pof.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z48587 mRNA Translation: CAA88488.1 AF244951 mRNA Translation: AAG23136.1 BC031741 mRNA Translation: AAH31741.1 |
CCDSi | CCDS26255.1 |
RefSeqi | NP_062364.3, NM_019491.5 |
3D structure databases
AlphaFoldDBi | P63321 |
SMRi | P63321 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 207790, 7 interactors |
IntActi | P63321, 1 interactor |
STRINGi | 10090.ENSMUSP00000009003 |
PTM databases
iPTMneti | P63321 |
PhosphoSitePlusi | P63321 |
SwissPalmi | P63321 |
Proteomic databases
EPDi | P63321 |
jPOSTi | P63321 |
MaxQBi | P63321 |
PaxDbi | P63321 |
PeptideAtlasi | P63321 |
PRIDEi | P63321 |
ProteomicsDBi | 253169 |
Protocols and materials databases
Antibodypediai | 26723, 371 antibodies from 35 providers |
DNASUi | 56044 |
Genome annotation databases
Ensembli | ENSMUST00000009003; ENSMUSP00000009003; ENSMUSG00000008859 |
GeneIDi | 56044 |
KEGGi | mmu:56044 |
UCSCi | uc007pof.1, mouse |
Organism-specific databases
CTDi | 5898 |
MGIi | MGI:1927243, Rala |
VEuPathDBi | HostDB:ENSMUSG00000008859 |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
GeneTreei | ENSGT00940000155142 |
HOGENOMi | CLU_041217_9_8_1 |
InParanoidi | P63321 |
OMAi | QSRAQQW |
OrthoDBi | 1259506at2759 |
PhylomeDBi | P63321 |
TreeFami | TF312796 |
Miscellaneous databases
BioGRID-ORCSi | 56044, 2 hits in 74 CRISPR screens |
ChiTaRSi | Rala, mouse |
PROi | PR:P63321 |
RNActi | P63321, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000008859, Expressed in vestibular organ and 330 other tissues |
Genevisiblei | P63321, MM |
Family and domain databases
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR028412, Ral IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR24070, PTHR24070, 1 hit PTHR24070:SF174, PTHR24070:SF174, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RALA_MOUSE | |
Accessioni | P63321Primary (citable) accession number: P63321 Secondary accession number(s): P05810 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | May 25, 2022 | |
This is version 152 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families