UniProtKB - P63281 (UBC9_RAT)
SUMO-conjugating enzyme UBC9
Ube2i
Functioni
Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451 (By similarity).
Can catalyze the formation of poly-SUMO chains (By similarity).
Essential for nuclear architecture and chromosome segregation (By similarity).
Necessary for sumoylation of FOXL2 and KAT5 (By similarity).
Sumoylates p53/TP53 at 'Lys-386' (By similarity).
Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity (By similarity).
By similarity: protein sumoylation Pathwayi
This protein is involved in the pathway protein sumoylation, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 93 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 | |
Sitei | 100 – 101 | Substrate bindingBy similarity | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- bHLH transcription factor binding Source: RGD
- enzyme binding Source: RGD
- HLH domain binding Source: RGD
- protein C-terminus binding Source: RGD
- RING-like zinc finger domain binding Source: RGD
- small protein activating enzyme binding Source: RGD
- SUMO conjugating enzyme activity Source: RGD
- SUMO transferase activity Source: RGD
- transcription coregulator binding Source: RGD
- ubiquitin-protein transferase activity Source: RGD
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB-KW
- negative regulation of transcription, DNA-templated Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- positive regulation of DNA-binding transcription factor activity Source: RGD
- positive regulation of I-kappaB kinase/NF-kappaB signaling Source: RGD
- positive regulation of intracellular steroid hormone receptor signaling pathway Source: RGD
- positive regulation of SUMO transferase activity Source: RGD
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: RGD
- protein sumoylation Source: RGD
- regulation of signaling receptor activity Source: RGD
- ubiquitin-dependent protein catabolic process Source: RGD
Keywordsi
Molecular function | Transferase |
Biological process | Cell cycle, Cell division, Chromosome partition, Mitosis, Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-196791, Vitamin D (calciferol) metabolism R-RNO-3065678, SUMO is transferred from E1 to E2 (UBE2I, UBC9) R-RNO-3108214, SUMOylation of DNA damage response and repair proteins R-RNO-3108232, SUMO E3 ligases SUMOylate target proteins R-RNO-3232118, SUMOylation of transcription factors R-RNO-3232142, SUMOylation of ubiquitinylation proteins R-RNO-3899300, SUMOylation of transcription cofactors R-RNO-4085377, SUMOylation of SUMOylation proteins R-RNO-4090294, SUMOylation of intracellular receptors R-RNO-4551638, SUMOylation of chromatin organization proteins R-RNO-4570464, SUMOylation of RNA binding proteins R-RNO-4615885, SUMOylation of DNA replication proteins R-RNO-4655427, SUMOylation of DNA methylation proteins R-RNO-4755510, SUMOylation of immune response proteins R-RNO-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-RNO-5693607, Processing of DNA double-strand break ends R-RNO-5696395, Formation of Incision Complex in GG-NER R-RNO-8866904, Negative regulation of activity of TFAP2 (AP-2) family transcription factors R-RNO-9615933, Postmitotic nuclear pore complex (NPC) reformation |
UniPathwayi | UPA00886 |
Names & Taxonomyi
Protein namesi | Recommended name: SUMO-conjugating enzyme UBC9 (EC:2.3.2.-)Alternative name(s): RING-type E3 SUMO transferase UBC9 SUMO-protein ligase Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-conjugating enzyme UbcE2A1 Publication Ubiquitin-protein ligase I |
Gene namesi | Name:Ube2i Synonyms:Ubce9 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3926, Ube2i |
Subcellular locationi
Nucleus
- fibrillar center Source: RGD
- nuclear body Source: RGD
- nuclear envelope Source: RGD
- nucleus Source: RGD
- PML body Source: RGD
Other locations
- cytoplasm Source: RGD
- dendrite Source: RGD
- SUMO ligase complex Source: RGD
- sumoylated E2 ligase complex Source: RGD
- synapse Source: RGD
- transferase complex Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000082457 | 2 – 158 | SUMO-conjugating enzyme UBC9Add BLAST | 157 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Cross-linki | 18 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 18 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Cross-linki | 48 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 49 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 49 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 65 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 71 | Phosphoserine; by CDK1By similarity | 1 | |
Cross-linki | 101 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P63281 |
PaxDbi | P63281 |
PTM databases
iPTMneti | P63281 |
PhosphoSitePlusi | P63281 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000017907, Expressed in spleen and 20 other tissues |
Genevisiblei | P63281, RN |
Interactioni
Subunit structurei
Forms a complex with SENP6 and UBE2I in response to UV irradiation (By similarity).
Forms a tight complex with RANGAP1 and RANBP2 (By similarity).
Identified in a complex with SUMO2 and UBE2I, where one ZNF451 interacts with one UBE2I and two SUMO2 chains, one bound to the UBE2I active site and the other to another region of the same UBE2I molecule (By similarity).
Interacts with SETX (By similarity).
Interacts with HIPK1 and HIPK2 (By similarity).
Interacts with PPM1J (By similarity).
Interacts with RASD2 (PubMed:19498170).
Interacts with TCF3 (PubMed:9013644).
Interacts with NR2C1; the interaction promotes its sumoylation (By similarity).
Interacts with SIAH1 (By similarity).
Interacts with PARP (By similarity).
Interacts with various transcription factors such as TFAP2A, TFAP2B, and TFAP2C (By similarity).
Interacts with AR (By similarity).
Interacts with ETS1 (By similarity).
Interacts with SOX4 (By similarity).
Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B (By similarity).
Interacts with FOXL2 (By similarity).
Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain (By similarity).
Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains (By similarity).
Interacts with FHIT (By similarity).
Interacts with PRKRA and p53/TP53 (By similarity).
Interacts with UHRF2 (By similarity).
Interacts with NR3C1 and this interaction is enhanced in the presence of RWDD3 (By similarity).
Interacts with MTA1 (By similarity).
Interacts with ZNF451 (By similarity).
Interacts with CPEB3 (By similarity).
Interacts with SUMO1, SUMO2, and SUMO3 (By similarity).
Interacts with IPO13 (By similarity).
Interacts with DNMT1 (By similarity).
By similarity2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 4 | Interaction with RANBP2By similarity | 1 | |
Sitei | 25 | Interaction with RANBP2By similarity | 1 | |
Sitei | 57 | Interaction with RANBP2By similarity | 1 |
Binary interactionsi
P63281
With | #Exp. | IntAct |
---|---|---|
Grik2 [P42260] | 3 | EBI-7974723,EBI-7809795 |
GO - Molecular functioni
- bHLH transcription factor binding Source: RGD
- enzyme binding Source: RGD
- HLH domain binding Source: RGD
- protein C-terminus binding Source: RGD
- RING-like zinc finger domain binding Source: RGD
- small protein activating enzyme binding Source: RGD
- transcription coregulator binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247604, 13 interactors |
DIPi | DIP-47657N |
IntActi | P63281, 2 interactors |
MINTi | P63281 |
STRINGi | 10116.ENSRNOP00000024406 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 157 | UBC corePROSITE-ProRule annotationAdd BLAST | 154 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 13 – 18 | Interaction with SUMO1By similarity | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0424, Eukaryota |
GeneTreei | ENSGT00550000075088 |
HOGENOMi | CLU_030988_12_0_1 |
InParanoidi | P63281 |
OMAi | QEPAWRA |
OrthoDBi | 1522509at2759 |
PhylomeDBi | P63281 |
TreeFami | TF101122 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG
60 70 80 90 100
TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED
110 120 130 140 150
KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA
QAKKFAPS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2KB55 | A0A0G2KB55_RAT | SUMO-conjugating enzyme UBC9 | LOC102553386 Ube2i | 229 | Annotation score: | ||
A0A8I6AG56 | A0A8I6AG56_RAT | SUMO-conjugating enzyme UBC9 | Ube2i | 101 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54632 mRNA Translation: AAC98704.1 BC086324 mRNA Translation: AAH86324.1 BC086592 mRNA Translation: AAH86592.1 |
RefSeqi | NP_037182.1, NM_013050.1 XP_006245980.2, XM_006245918.3 XP_006245981.1, XM_006245919.3 XP_006245982.1, XM_006245920.3 XP_008765771.2, XM_008767549.2 XP_017452533.1, XM_017597044.1 |
Genome annotation databases
Ensembli | ENSRNOT00000104064; ENSRNOP00000085922; ENSRNOG00000066579 |
GeneIDi | 25573 |
KEGGi | rno:25573 |
UCSCi | RGD:3926, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54632 mRNA Translation: AAC98704.1 BC086324 mRNA Translation: AAH86324.1 BC086592 mRNA Translation: AAH86592.1 |
RefSeqi | NP_037182.1, NM_013050.1 XP_006245980.2, XM_006245918.3 XP_006245981.1, XM_006245919.3 XP_006245982.1, XM_006245920.3 XP_008765771.2, XM_008767549.2 XP_017452533.1, XM_017597044.1 |
3D structure databases
AlphaFoldDBi | P63281 |
BMRBi | P63281 |
SMRi | P63281 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247604, 13 interactors |
DIPi | DIP-47657N |
IntActi | P63281, 2 interactors |
MINTi | P63281 |
STRINGi | 10116.ENSRNOP00000024406 |
PTM databases
iPTMneti | P63281 |
PhosphoSitePlusi | P63281 |
Proteomic databases
jPOSTi | P63281 |
PaxDbi | P63281 |
Genome annotation databases
Ensembli | ENSRNOT00000104064; ENSRNOP00000085922; ENSRNOG00000066579 |
GeneIDi | 25573 |
KEGGi | rno:25573 |
UCSCi | RGD:3926, rat |
Organism-specific databases
CTDi | 7329 |
RGDi | 3926, Ube2i |
Phylogenomic databases
eggNOGi | KOG0424, Eukaryota |
GeneTreei | ENSGT00550000075088 |
HOGENOMi | CLU_030988_12_0_1 |
InParanoidi | P63281 |
OMAi | QEPAWRA |
OrthoDBi | 1522509at2759 |
PhylomeDBi | P63281 |
TreeFami | TF101122 |
Enzyme and pathway databases
UniPathwayi | UPA00886 |
Reactomei | R-RNO-196791, Vitamin D (calciferol) metabolism R-RNO-3065678, SUMO is transferred from E1 to E2 (UBE2I, UBC9) R-RNO-3108214, SUMOylation of DNA damage response and repair proteins R-RNO-3108232, SUMO E3 ligases SUMOylate target proteins R-RNO-3232118, SUMOylation of transcription factors R-RNO-3232142, SUMOylation of ubiquitinylation proteins R-RNO-3899300, SUMOylation of transcription cofactors R-RNO-4085377, SUMOylation of SUMOylation proteins R-RNO-4090294, SUMOylation of intracellular receptors R-RNO-4551638, SUMOylation of chromatin organization proteins R-RNO-4570464, SUMOylation of RNA binding proteins R-RNO-4615885, SUMOylation of DNA replication proteins R-RNO-4655427, SUMOylation of DNA methylation proteins R-RNO-4755510, SUMOylation of immune response proteins R-RNO-5693565, Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks R-RNO-5693607, Processing of DNA double-strand break ends R-RNO-5696395, Formation of Incision Complex in GG-NER R-RNO-8866904, Negative regulation of activity of TFAP2 (AP-2) family transcription factors R-RNO-9615933, Postmitotic nuclear pore complex (NPC) reformation |
Miscellaneous databases
PROi | PR:P63281 |
Gene expression databases
Bgeei | ENSRNOG00000017907, Expressed in spleen and 20 other tissues |
Genevisiblei | P63281, RN |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | UBC9_RAT | |
Accessioni | P63281Primary (citable) accession number: P63281 Secondary accession number(s): P50550, Q15698, Q86VB3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 11, 2004 |
Last sequence update: | October 11, 2004 | |
Last modified: | May 25, 2022 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families